| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88861-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-2860-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80611-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19182-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74167 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61780-0.025ml | |||
![Western Blot: PARD3/Par3 Antibody [NBP1-88861] - Analysis in human cell lines A-549 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Western-Blot-NBP1-88861-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: PARD3/Par3 Antibody [NBP1-88861] - Analysis in human cell lines A-549 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![<b>Orthogonal Strategies Validation. </b>Simple Western: PARD3/Par3 Antibody [NBP1-88861] - Simple Western lane view shows a specific band for PARD3/Par3 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Simple-Western-NBP1-88861-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cell line U-2 OS shows localization to cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88861-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - Staining PARD3 in mouse trophoblast stem cells. Verified customer review from 1DegreeBio.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88861-img0012.jpg)
![Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human colon shows distinct luminal membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - MCAM is required to establish cell autonomous polarity. In wild-type myotubes PAR3 remains cytoplasmic. MCAM contributes to the establishment of cell autonomous polarity in myogenic and chondrogenic differentiation. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28923978/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88861-img0023.jpg)
![Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human skin shows weak to moderate cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cell line 8505C cells (anaplastic thyroid cancer cell line). Image from verified customer review.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88861-img0013.jpg)
![Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human kidney shows weak to moderate membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0019.jpg)
![Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cerebellum shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0020.jpg)
![Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human fallopian tube shows weak to moderate positivity in luminal membrane in glandular cells.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0021.jpg)
![Simple Western: PARD3/Par3 Antibody [NBP1-88861] - Electropherogram image(s) of corresponding Simple Western lane view. PARD3/Par3 antibody was used at 1:100 dilution on RT-4 lysate(s).](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Simple-Western-NBP1-88861-img0015.jpg)
![Immunocytochemistry/ Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - MCAM is required to establish cell autonomous polarity. (A) In elongating myotubes (10T1/2 cells treated with testosterone for 7 days) VANGL2 is localized asymmetrically at the tip of the cell. (B) The VANGL2 enriched tip of the cell is marked by MSN. (C) In MCAM knockout C164 cells myotube elongation fails, MSN labels the whole plasma membrane & VANGL2 is spread across the cytoplasm. (D) Highly polarized localization of MCAM & SCRIB at the distal end of growing wild-type myotube. Separate channels of the boxed area are shown on the right. (E) In MCAM knockout cells SCRIB levels remain low & it is spread evenly in the cell. (F) In wild-type myotubes PAR3 remains cytoplasmic, whereas (G) in MCAM knockout C164 cells it can be detected at the cell cortex. (H) RT-qPCR demonstrates reduced expression of Scrib in MCAM mutant cell lines. Cells were treated for 7 days with BMP2 or testosterone (n=3; **P<0.01; ***P<0.001; two-tailed t-test; mean±s.e.m.). (I) Deletion of MCAM endocytosis motif leads to similar polarity defects as complete MCAM elimination. VANGL2 is evenly spread in U125 cells & PAR3 accumulates in cell cortex. (J) In chondrogenic differentiation VANGL2 was observed asymmetrically in limited number of cells. In MCAM mutant cell lines (C149, C164, U125) VANGL2 accumulated around the nucleus. (K) Initiation of myogenic (4-day culture with testosterone) & chondrogenic differentiation (4-day culture with BMP2) led to downregulation of ERK1/2 phosphorylation (p-ERK1/2). Instead in MCAM mutant cell lines ERK1/2 phosphorylation increased. Scale bars: 25 µm. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/doi/10.1242/bio.027771/256759/MCAM-contributes-to-the-establishment-of-cell), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88861_rabbit-polyclonal-pard3-par3-antibody-310202416113535.jpg)
![Immunocytochemistry/ Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - MCAM is required to establish cell autonomous polarity. (A) In elongating myotubes (10T1/2 cells treated with testosterone for 7 days) VANGL2 is localized asymmetrically at the tip of the cell. (B) The VANGL2 enriched tip of the cell is marked by MSN. (C) In MCAM knockout C164 cells myotube elongation fails, MSN labels the whole plasma membrane & VANGL2 is spread across the cytoplasm. (D) Highly polarized localization of MCAM & SCRIB at the distal end of growing wild-type myotube. Separate channels of the boxed area are shown on the right. (E) In MCAM knockout cells SCRIB levels remain low & it is spread evenly in the cell. (F) In wild-type myotubes PAR3 remains cytoplasmic, whereas (G) in MCAM knockout C164 cells it can be detected at the cell cortex. (H) RT-qPCR demonstrates reduced expression of Scrib in MCAM mutant cell lines. Cells were treated for 7 days with BMP2 or testosterone (n=3; **P<0.01; ***P<0.001; two-tailed t-test; mean±s.e.m.). (I) Deletion of MCAM endocytosis motif leads to similar polarity defects as complete MCAM elimination. VANGL2 is evenly spread in U125 cells & PAR3 accumulates in cell cortex. (J) In chondrogenic differentiation VANGL2 was observed asymmetrically in limited number of cells. In MCAM mutant cell lines (C149, C164, U125) VANGL2 accumulated around the nucleus. (K) Initiation of myogenic (4-day culture with testosterone) & chondrogenic differentiation (4-day culture with BMP2) led to downregulation of ERK1/2 phosphorylation (p-ERK1/2). Instead in MCAM mutant cell lines ERK1/2 phosphorylation increased. Scale bars: 25 µm. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/doi/10.1242/bio.027771/256759/MCAM-contributes-to-the-establishment-of-cell), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88861_rabbit-polyclonal-pard3-par3-antibody-310202416113539.jpg)
![Western Blot: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01] - Analysis of PTN expression in transfected 293T cell line by PTN monoclonal antibody (M01), clone 5C3.Lane 1: PTN transfected lysate(18.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Pleiotrophin-PTN-Antibody-5C3-Western-Blot-H00005764-M01-img0008.jpg "Western Blot: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01] - Analysis of PTN expression in transfected 293T cell line by PTN monoclonal antibody (M01), clone 5C3.Lane 1: PTN transfected lysate(18.9 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01] - Analysis of monoclonal antibody to PTN on formalin-fixed paraffin-embedded human cerebral cortex. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Pleiotrophin-PTN-Antibody-5C3-Immunohistochemistry-Paraffin-H00005764-M01-img0006.jpg)
![Western Blot: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01] - PTN monoclonal antibody (M01), clone 5C3 Analysis of PTN expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Pleiotrophin-PTN-Antibody-5C3-Western-Blot-H00005764-M01-img0007.jpg)
![Western Blot: Calmodulin Antibody (2D1) [NB120-2860] - Purified Calmodulin using NB120-2860 and NB120-5494.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Western-Blot-NB120-2860-img0028.jpg "Western Blot: Calmodulin Antibody (2D1) [NB120-2860] - Purified Calmodulin using NB120-2860 and NB120-5494.")
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Staining of rat brain.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NB120-2860-img0008.jpg)
![Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) [NB120-2860] - Normal biopsies of deparaffinized Rat testis tissue.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunohistochemistry-Paraffin-NB120-2860-img0013.jpg)
![Flow Cytometry: Calmodulin Antibody (2D1) [NB120-2860] - Analysis of Calmodulin in MCF-7 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Flow-Cytometry-NB120-2860-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.A2058 melanoma cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NB120-2860-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. C6 glioma cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NB120-2860-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. HeLa Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NB120-2860-img0017.jpg)
![Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) [NB120-2860] - Normal biopsies of deparaffinized Rat cerebellum tissue.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunohistochemistry-Paraffin-NB120-2860-img0012.jpg)
![Flow Cytometry: Calmodulin Antibody (2D1) [NB120-2860] - Analysis of Calmodulin in C6 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Flow-Cytometry-NB120-2860-img0018.jpg)
![Flow Cytometry: Calmodulin Antibody (2D1) [NB120-2860] - Analysis of Calmodulin in PC12 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Flow-Cytometry-NB120-2860-img0019.jpg)
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Knockdown efficiencies of target proteins in HEK293 cells measured by Reverse Phase Protein Array (RPPA) and Western blotting (WB). Level of expression by WB and RPPA of targeted proteins in HEK293 cells transfected with siRNA targeting proteins of interest or non-silencing siRNA control (siCTR). Specific bands for WB and spots corresponding to the dilution of the dynamic range in RPPA are presented. Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Knockdown-Validated-H00005997-M01-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Knockdown efficiencies of target proteins in HEK293 cells measured by Reverse Phase Protein Array (RPPA) and Western blotting (WB). Level of expression by WB and RPPA of targeted proteins in HEK293 cells transfected with siRNA targeting proteins of interest or non-silencing siRNA control (siCTR). Specific bands for WB and spots corresponding to the dilution of the dynamic range in RPPA are presented. Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Specificity of antibodies. HEK293 cells lysates were analysed by Western blotting to test the specificity of antibodies. Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Western-Blot-H00005997-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: RGS2 Antibody (4C4) [H00005997-M01] - Analysis of monoclonal antibody to RGS2 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Immunohistochemistry-Paraffin-H00005997-M01-img0005.jpg)
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - RGS2 monoclonal antibody (M01), clone 4C4 Analysis of RGS2 expression in MCF-7.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Western-Blot-H00005997-M01-img0007.jpg)
![ELISA: RGS2 Antibody (4C4) [H00005997-M01] - Detection limit for recombinant GST tagged RGS2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-ELISA-H00005997-M01-img0008.jpg)
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Specificity of antibodies. HEK293 cells lysates were analysed by Western blotting to test the specificity of antibodies. Image collected & cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005997-m01_mouse-monoclonal-rgs2-antibody-4c4-310202415394536.jpg)
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Knockdown efficiencies of target proteins in HEK293 cells measured by Reverse Phase Protein Array (RPPA) & Western blotting (WB). A. Detection by RPPA of VCL & ERK2 in serial dilution of HEK293 cells transfected with siRNA targeting VCL (siVCL) or non-silencing siRNA control (siCTR). B. Linear regression was performed for ERK2 & VCL protein & Pearson correlation between dilution & the signal intensity was calculated for each condition of Figure 3A, with all p-values lower than 0.001. Signal intensities are expressed as mean of the replicates. C. Level of expression by WB & RPPA of targeted proteins in HEK293 cells transfected with siRNA targeting proteins of interest or non-silencing siRNA control (siCTR). Specific bands for WB & spots corresponding to the dilution of the dynamic range in RPPA are presented. Image collected & cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005997-m01_mouse-monoclonal-rgs2-antibody-4c4-310202415482256.jpg)
![Western Blot: BRMS1 Antibody (2D4-2G11) [H00025855-M01] - BRMS1 monoclonal antibody (M01), clone 2D4-2G11. Analysis of BRMS1 expression in human liver.](http://images.novusbio.com/fullsize/BRMS1-Antibody-2D4-2G11-Western-Blot-H00025855-M01-img0005.jpg "Western Blot: BRMS1 Antibody (2D4-2G11) [H00025855-M01] - BRMS1 monoclonal antibody (M01), clone 2D4-2G11. Analysis of BRMS1 expression in human liver.")
![Western Blot: BRMS1 Antibody (2D4-2G11) [H00025855-M01] - Analysis of BRMS1 expression in transfected 293T cell line by BRMS1 monoclonal antibody (M01), clone 2D4-2G11.Lane 1: BRMS1 transfected lysate (Predicted MW: 28.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/BRMS1-Antibody-2D4-2G11-Western-Blot-H00025855-M01-img0006.jpg)
![Immunoprecipitation: BRMS1 Antibody (2D4-2G11) [H00025855-M01] - Analysis of BRMS1 transfected lysate using anti-BRMS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BRMS1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/BRMS1-Antibody-2D4-2G11-Immunoprecipitation-H00025855-M01-img0003.jpg)
![Sandwich ELISA: BRMS1 Antibody (2D4-2G11) [H00025855-M01] - Detection limit for recombinant GST tagged BRMS1 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/BRMS1-Antibody-2D4-2G11-Sandwich-ELISA-H00025855-M01-img0004.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Analysis in human cerebral cortex and pancreas tissues. Corresponding YWHAB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Analysis in human cerebral cortex and pancreas tissues. Corresponding YWHAB RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: 14-3-3 beta/alpha Antibody [NBP1-80611] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NBP1-80611-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80611-img0003.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human pancreas shows very weak positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0010.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody [NBP1-80611] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NBP1-80611-img0004.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0007.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human skin shows moderate to strong cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0009.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Analysis in human testis and liver tissues using NBP1-84416 antibody. Corresponding RBM14 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Analysis in human testis and liver tissues using NBP1-84416 antibody. Corresponding RBM14 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RBM14 Antibody [NBP1-84416] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84416-img0009.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0013.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0011.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0012.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0014.jpg)
![Western Blot: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Analysis of Calmodulin on rat brain lysates using anti-Calmodulin antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-SJ16-09-Western-Blot-NBP2-67413-img0001.jpg "Western Blot: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Analysis of Calmodulin on rat brain lysates using anti-Calmodulin antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413] - NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-SJ16-09-Immunocytochemistry-Immunofluorescence-NBP2-67413-img0005.jpg)
![Immunohistochemistry-Paraffin: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-Calmodulin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-SJ16-09-Immunohistochemistry-Paraffin-NBP2-67413-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-SJ16-09-Immunocytochemistry-Immunofluorescence-NBP2-67413-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413] - MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-SJ16-09-Immunocytochemistry-Immunofluorescence-NBP2-67413-img0004.jpg)
![Immunohistochemistry-Paraffin: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Analysis of paraffin-embedded mouse brain tissue using anti-Calmodulin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-SJ16-09-Immunohistochemistry-Paraffin-NBP2-67413-img0006.jpg)
![Western Blot: HGF Antibody (7-2) [NBP1-19182] - Lane 1 hHGF 1ug, Lane 2 hHGF 0.5ug and Lane 3 hHGF 0.25ug.](http://images.novusbio.com/fullsize/HGF-Antibody-7-2-Western-Blot-NBP1-19182-img0008.jpg "Western Blot: HGF Antibody (7-2) [NBP1-19182] - Lane 1 hHGF 1ug, Lane 2 hHGF 0.5ug and Lane 3 hHGF 0.25ug.")
![Immunohistochemistry-Paraffin: HGF Antibody (7-2) [NBP1-19182] - Classification of liposarcomas generated in the Ptenflox/flox; p53flox/flox mouse model. Different subtypes of liposarcoma are characterized by a unique lipocytic phenotype. Lipoprotein lipase (LPL) is an adipocytic marker and stains more mature adipocytic sarcomas such as WDLPS and MLPS. Hepatocyte growth factor (HGF) is a mesenchymal stem cell marker and stains immature liposarcomas such as DDLPS and PLPS. Scale bar corresponds to 50 um Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/cdd.2015.27) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HGF-Antibody-7-2-Immunohistochemistry-Paraffin-NBP1-19182-img0009.jpg)
![Immunohistochemistry-Paraffin: HGF Antibody (7-2) [NBP1-19182] - Human breast tissue stained with HGF/SF, mAb (7-2) at 10 ug/ml.](http://images.novusbio.com/fullsize/HGF-Antibody-7-2-Immunohistochemistry-Paraffin-NBP1-19182-img0005.jpg)
![Immunohistochemistry-Paraffin: HGF Antibody (7-2) [NBP1-19182] - Human breast tissue stained with HGF/SF, mAb (7-2) at 10ug/ml.](http://images.novusbio.com/fullsize/HGF-Antibody-7-2-Immunohistochemistry-Paraffin-NBP1-19182-img0007.jpg)
![Western Blot: SHC1 Antibody (OTI3A1) [NBP2-02434] - Figure from citation:(p66shc) protein level by using anti-SHC1 antibody in human HBL-100/MCF-7 cell lysates.](http://images.novusbio.com/fullsize/SHC1-Antibody-OTI3A1-Western-Blot-NBP2-02434-img0011.jpg "Western Blot: SHC1 Antibody (OTI3A1) [NBP2-02434] - Figure from citation:(p66shc) protein level by using anti-SHC1 antibody in human HBL-100/MCF-7 cell lysates.")
![Immunocytochemistry/Immunofluorescence: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SHC1 .](http://images.novusbio.com/fullsize/SHC1-Antibody-OTI3A1-Immunocytochemistry-Immunofluorescence-NBP2-02434-img0010.jpg)
![Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded prostate tissue using anti-SHC1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SHC1-Antibody-OTI3A1-Immunohistochemistry-Paraffin-NBP2-02434-img0009.jpg)
![Western Blot: SHC1 Antibody (OTI3A1) [NBP2-02434] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHC1.](http://images.novusbio.com/fullsize/SHC1-Antibody-OTI3A1-Western-Blot-NBP2-02434-img0001.jpg)
![Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded bladder tissue using anti-SHC1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SHC1-Antibody-OTI3A1-Immunohistochemistry-Paraffin-NBP2-02434-img0002.jpg)
![Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded breast tissue using anti-SHC1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SHC1-Antibody-OTI3A1-Immunohistochemistry-Paraffin-NBP2-02434-img0003.jpg)
![Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-SHC1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SHC1-Antibody-OTI3A1-Immunohistochemistry-Paraffin-NBP2-02434-img0004.jpg)
![Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-SHC1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SHC1-Antibody-OTI3A1-Immunohistochemistry-Paraffin-NBP2-02434-img0005.jpg)
![Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded colon tissue using anti-SHC1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SHC1-Antibody-OTI3A1-Immunohistochemistry-Paraffin-NBP2-02434-img0006.jpg)
![Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded Kidney tissue using anti-SHC1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SHC1-Antibody-OTI3A1-Immunohistochemistry-Paraffin-NBP2-02434-img0007.jpg)
![Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded lung tissue using anti-SHC1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SHC1-Antibody-OTI3A1-Immunohistochemistry-Paraffin-NBP2-02434-img0008.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mAb against HEK293 (1) and YWHAB (AA: 1-246)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Western-Blot-NBP2-61780-img0009.jpg "Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mAb against HEK293 (1) and YWHAB (AA: 1-246)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of HepG2 cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunocytochemistry-Immunofluorescence-NBP2-61780-img0005.jpg)
![Immunohistochemistry: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of paraffin-embedded endometrial cancer tissues using YWHAB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunohistochemistry-NBP2-61780-img0007.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mAb against human YWHAB (AA: 1-246) recombinant protein. (Expected MW is 54 kDa)](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Western-Blot-NBP2-61780-img0008.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mouse mAb against Hela (1), NIH/3T3 (2), C6 (3), A431 (4), K562 (5), PC-12 (6), and U937 (7) cell lysate.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Western-Blot-NBP2-61780-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of GC-7901 cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunocytochemistry-Immunofluorescence-NBP2-61780-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of Hela cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunocytochemistry-Immunofluorescence-NBP2-61780-img0004.jpg)
![Immunohistochemistry: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of paraffin-embedded rectum cancer tissues using YWHAB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunohistochemistry-NBP2-61780-img0006.jpg)
![ELISA: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-ELISA-NBP2-61780-img0001.jpg)
![ELISA: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of Hela cells using YWHAB mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-ELISA-NBP2-61780-img0002.jpg)
![Western Blot: SHC1 Antibody (3F4) [H00006464-M01] - SHC1 monoclonal antibody (M01), clone 3F4 Analysis of SHC1 expression in A-431.](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-Western-Blot-H00006464-M01-img0015.jpg "Western Blot: SHC1 Antibody (3F4) [H00006464-M01] - SHC1 monoclonal antibody (M01), clone 3F4 Analysis of SHC1 expression in A-431.")
![Immunocytochemistry/Immunofluorescence: SHC1 Antibody (3F4) [H00006464-M01] - Analysis of monoclonal antibody to SHC1 on A-431 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-Immunocytochemistry-Immunofluorescence-H00006464-M01-img0011.jpg)
![Immunohistochemistry-Paraffin: SHC1 Antibody (3F4) [H00006464-M01] - Analysis of monoclonal antibody to SHC1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-Immunohistochemistry-Paraffin-H00006464-M01-img0013.jpg)
![Proximity Ligation Assay: SHC1 Antibody (3F4) [H00006464-M01] - Protein-protein interactions between STAT5A and SHC1 Mahlavu cells were stained with anti-STAT5A rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-Proximity-Ligation-Assay-H00006464-M01-img0007.jpg)
![Proximity Ligation Assay: SHC1 Antibody (3F4) [H00006464-M01] - Analysis of protein-protein interactions between PIK3R1 and SHC1. HeLa cells were stained with anti-PIK3R1 rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-Proximity-Ligation-Assay-H00006464-M01-img0008.jpg)
![Proximity Ligation Assay: SHC1 Antibody (3F4) [H00006464-M01] - Analysis of protein-protein interactions between PIK3R1 and SHC1. Huh7 cells were stained with anti-PIK3R1 rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-Proximity-Ligation-Assay-H00006464-M01-img0009.jpg)
![ELISA: SHC1 Antibody (3F4) [H00006464-M01] - Detection limit for recombinant GST tagged SHC1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-ELISA-H00006464-M01-img0016.jpg)