| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-101SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-330SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-439SS | |||
| Azide and BSA Free | NBP2-80945 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90134-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92870-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92622-0.02ml | |||
![Immunocytochemistry/ Immunofluorescence: CENPF Antibody - BSA Free [NB500-101] - Kinetochore localization of RZZ and MAD1 are independent of CENP-E and CENP-F. CENP-F depletion does not interfere with the recruitment of Zwilch. The graphs show mean intensity of one (B, C, and E), two (D and F), or three (A, G, and H) experiments; the error bars indicate S.E., and the mean values for nondepleted cells are set to 1. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29748388/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CENPF-Antibody-Immunohistochemistry-Paraffin-NB500-101-img0009.jpg "<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: CENPF Antibody - BSA Free [NB500-101] - Kinetochore localization of RZZ and MAD1 are independent of CENP-E and CENP-F. CENP-F depletion does not interfere with the recruitment of Zwilch. The graphs show mean intensity of one (B, C, and E), two (D and F), or three (A, G, and H) experiments; the error bars indicate S.E., and the mean values for nondepleted cells are set to 1. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29748388/) licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: CENPF Antibody [NB500-101] - 25ug of asynchronous and mitotically blocked HeLa lysates were loaded in each respective lane.](http://images.novusbio.com/fullsize/CENPF-Antibody-Western-Blot-NB500-101-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CENPF Antibody [NB500-101] - Distribution of CENP-F (red), largely associated with the mitotic spindle and a clearly distinguishable frac.](http://images.novusbio.com/fullsize/CENPF-Antibody-Immunocytochemistry-Immunofluorescence-NB500-101-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CENPF Antibody [NB500-101] - CENPF antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/CENPF-Antibody-Immunocytochemistry-Immunofluorescence-NB500-101-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CENPF Antibody [NB500-101] - Confocal immunofluorescent analysis of HeLa cells using CENPF antibody (NB500-101, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).](http://images.novusbio.com/fullsize/CENPF-Antibody-Immunocytochemistry-Immunofluorescence-NB500-101-img0007.jpg)
![Western Blot: Separase Antibody (6H6) [H00009700-M01] - ESPL1 monoclonal antibody (M01), clone 6H6 Analysis of ESPL1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Separase-Antibody-6H6-Western-Blot-H00009700-M01-img0005.jpg "Western Blot: Separase Antibody (6H6) [H00009700-M01] - ESPL1 monoclonal antibody (M01), clone 6H6 Analysis of ESPL1 expression in Hela S3 NE.")
![Immunohistochemistry-Paraffin: Separase Antibody (6H6) [H00009700-M01] - Analysis of monoclonal antibody to ESPL1 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Separase-Antibody-6H6-Immunohistochemistry-Paraffin-H00009700-M01-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Separase Antibody (XJ11-4D7) [NB100-330] - Overlay [blue] of centrosomal staining in HeLa cells. Centrosomal staining of separase [yellow], using NB 100-330, and gamma tubulin [green] in mitotic-metaphase cells and nuclear staining of separase in pre-mitotic cells.](http://images.novusbio.com/fullsize/Separase-Antibody-XJ11-4D7-Immunocytochemistry-Immunofluorescence-NB100-330-img0003.jpg "Immunocytochemistry/Immunofluorescence: Separase Antibody (XJ11-4D7) [NB100-330] - Overlay [blue] of centrosomal staining in HeLa cells. Centrosomal staining of separase [yellow], using NB 100-330, and gamma tubulin [green] in mitotic-metaphase cells and nuclear staining of separase in pre-mitotic cells.")
![Western Blot: CDK5 Activator 1 Antibody [NBP3-13279] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with CDK5 Activator 1 antibody (NBP3-13279) diluted at 1:1000 and competitor's antibody diluted by 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CDK5-Activator-1-Antibody-Western-Blot-NBP3-13279-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CDK5 Activator 1 Antibody [NBP3-13279] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with CDK5 Activator 1 antibody (NBP3-13279) diluted at 1:1000 and competitor's antibody diluted by 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: CDK5 Activator 1 Antibody [NBP3-13279] - CDK5 Activator 1 antibody detects CDK5 Activator 1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDK5 Activator 1 stained by CDK5 Activator 1 antibody (NBP3-13279) diluted at 1:500. Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/CDK5-Activator-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13279-img0001.jpg)
![Immunohistochemistry-Paraffin: CDK5 Activator 1 Antibody [NBP3-13279] - CDK5 Activator 1 antibody detects CDK5 Activator 1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. CDK5 Activator 1 stained by CDK5 Activator 1 antibody (NBP3-13279) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/CDK5-Activator-1-Antibody-Immunohistochemistry-Paraffin-NBP3-13279-img0003.jpg)
![Immunohistochemistry-Paraffin: CDK5 Activator 1 Antibody [NBP3-13279] - CDK5 Activator 1 antibody detects CDK5 Activator 1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CDK5 Activator 1 stained by CDK5 Activator 1 antibody (NBP3-13279) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/CDK5-Activator-1-Antibody-Immunohistochemistry-Paraffin-NBP3-13279-img0002.jpg)
![Western Blot: Separase Antibody (XJ11-1B12) [NB100-439] - Separase detected in 5C6 cell lysates using NB 100-439. Lane 1: Non-induced lysate. Lane 2: Lysate induced with ponasterone.](http://images.novusbio.com/fullsize/Separase-Antibody-XJ11-1B12-Western-Blot-NB100-439-img0002.jpg "Western Blot: Separase Antibody (XJ11-1B12) [NB100-439] - Separase detected in 5C6 cell lysates using NB 100-439. Lane 1: Non-induced lysate. Lane 2: Lysate induced with ponasterone.")
![Immunohistochemistry-Paraffin: APEG1 Antibody [NBP1-90134] - Analysis in human skeletal muscle and liver tissues using NBP1-90134 antibody. Corresponding SPEG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/APEG1-Antibody-Immunohistochemistry-Paraffin-NBP1-90134-img0015.jpg "Immunohistochemistry-Paraffin: APEG1 Antibody [NBP1-90134] - Analysis in human skeletal muscle and liver tissues using NBP1-90134 antibody. Corresponding SPEG RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: APEG1 Antibody [NBP1-90134] - Staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/APEG1-Antibody-Immunohistochemistry-Paraffin-NBP1-90134-img0010.jpg)
![Immunohistochemistry-Paraffin: APEG1 Antibody [NBP1-90134] - Staining of human endometrium shows strong membranous positivity in stromal cells.](http://images.novusbio.com/fullsize/APEG1-Antibody-Immunohistochemistry-Paraffin-NBP1-90134-img0012.jpg)
![Immunohistochemistry-Paraffin: APEG1 Antibody [NBP1-90134] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/APEG1-Antibody-Immunohistochemistry-Paraffin-NBP1-90134-img0013.jpg)
![Immunohistochemistry-Paraffin: APEG1 Antibody [NBP1-90134] - Staining of human skeletal muscle shows moderate membranous positivity in striated muscle fibers.](http://images.novusbio.com/fullsize/APEG1-Antibody-Immunohistochemistry-Paraffin-NBP1-90134-img0014.jpg)
![Western Blot: CDK5RAP1 Antibody [NBP2-92870] - Analysis of extracts of various cell lines, using CDK5RAP1 .Exposure time: 30s.](http://images.novusbio.com/fullsize/CDK5RAP1-Antibody-Western-Blot-NBP2-92870-img0006.jpg "Western Blot: CDK5RAP1 Antibody [NBP2-92870] - Analysis of extracts of various cell lines, using CDK5RAP1 .Exposure time: 30s.")
![Immunohistochemistry-Paraffin: CDK5RAP1 Antibody [NBP2-92870] - Rat liver using CDK5RAP1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CDK5RAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-92870-img0005.jpg)
![Immunohistochemistry-Paraffin: CDK5RAP1 Antibody [NBP2-92870] - Human colon carcinoma using CDK5RAP1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CDK5RAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-92870-img0003.jpg)
![Immunohistochemistry-Paraffin: CDK5RAP1 Antibody [NBP2-92870] - Mouse kidney using CDK5RAP1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CDK5RAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-92870-img0004.jpg)
![Western Blot: CDK5RAP1 Antibody [NBP2-15844] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CDK5RAP1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/CDK5RAP1-Antibody-Western-Blot-NBP2-15844-img0005.jpg "Western Blot: CDK5RAP1 Antibody [NBP2-15844] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CDK5RAP1 antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: CDK5RAP1 Antibody [NBP2-15844] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CDK5RAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15844-img0003.jpg)
![Immunohistochemistry-Paraffin: CDK5RAP1 Antibody [NBP2-15844] - Paraffin-embedded Rat brain. CDK5RAP1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/CDK5RAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-15844-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CDK5 Activator 1 Antibody [NBP2-92622] - Analysis of SH-SY5Y cells using CDK5 Activator 1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CDK5-Activator-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92622-img0001.jpg "Immunocytochemistry/Immunofluorescence: CDK5 Activator 1 Antibody [NBP2-92622] - Analysis of SH-SY5Y cells using CDK5 Activator 1 at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Western Blot: CDK5 Activator 1 Antibody - Azide and BSA Free [NBP2-92622] - Western blot analysis of various lysates, using CDK5 Activator 1 antibody at 1:700 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-92622_rabbit-polyclonal-cdk5-activator-1-antibody-422025143186.jpg%3Ft=1690973940)
![Western Blot: CENPF Antibody (PSH0-79) [NBP3-32181] - Western blot analysis of CENPF on different lysates with Rabbit anti-CENPF antibody (NBP3-32181) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 358 kDa Observed band size: 358 kDa Exposure time: 3 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32181) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32181_rabbit-cenpf-mab-psh0-79-278202416331287.jpg "Western Blot: CENPF Antibody (PSH0-79) [NBP3-32181] - Western blot analysis of CENPF on different lysates with Rabbit anti-CENPF antibody (NBP3-32181) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 358 kDa Observed band size: 358 kDa Exposure time: 3 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32181) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CENPF Antibody (PSH0-79) [NBP3-32181] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CENPF antibody (NBP3-32181) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32181) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32181_rabbit-cenpf-mab-psh0-79-298202414262657.jpg)