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![Western Blot: CSN1 Antibody [NB100-362] - Detection of Human CSN1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CSN1 antibody NB100-362 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CSN1-Antibody-Western-Blot-NB100-362-img0017.jpg "<b>Biological Strategies Validation. </b>Western Blot: CSN1 Antibody [NB100-362] - Detection of Human CSN1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CSN1 antibody NB100-362 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: CSN1 Antibody [NB100-362] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CSN1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CSN1-Antibody-Immunohistochemistry-NB100-362-img0015.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CSN1 Antibody [NB100-362] - Whole cell lysate (50 ug - E; 10 ug - T) from mock transfected (E) or CSN1 transfected (T) HEK293T cells. Antibody used at the indicated concentration.](http://images.novusbio.com/fullsize/CSN1-Antibody-Western-Blot-NB100-362-img0010.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CSN1 Antibody [NB100-362] - Samples : Whole cell lysate (50 ug - E; 10 ug - T) from mock transfected (E) or CSN1 transfected (T) HEK293T cells. Antibody : Affinity purified rabbit anti-CSN1 used at the indicated concentration. Detection : Chemiluminescence with a 10 second exposure.](http://images.novusbio.com/fullsize/CSN1-Antibody-Western-Blot-NB100-362-img0014.jpg)
![Immunohistochemistry-Paraffin: CSN1 Antibody [NB100-362] - FFPE section of human prostate-nodular hypertrophy. Affinity purified rabbit anti-CSN1 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit](http://images.novusbio.com/fullsize/CSN1-Antibody-Immunohistochemistry-Paraffin-NB100-362-img0008.jpg)
![Western Blot: Centrin 1 Antibody [NBP2-14909] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin 1 antibody diluted at a dilution of 1:1000.](http://images.novusbio.com/fullsize/Centrin-1-Antibody-Western-Blot-NBP2-14909-img0003.jpg "Western Blot: Centrin 1 Antibody [NBP2-14909] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin 1 antibody diluted at a dilution of 1:1000.")
![Immunoprecipitation: Centrin 1 Antibody [NBP2-14909] - Immunoprecipitation of Centrin-1 protein from 293T whole cell extracts using 5 ug of Centrin-1 antibody [N1C3] . Western blot analysis was performed using Centrin-1 antibody [N1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Centrin-1-Antibody-Immunoprecipitation-NBP2-14909-img0006.jpg)
![Immunoprecipitation: Centrin 1 Antibody [NBP2-14909] - A431 cells were fixed in ice-cold MeOH for 5 min.Green: Centrin 1 stained by Centrin 1 antibody [N1C3] diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/antibody/nbp2-14909_rabbit-polyclonal-centrin-1-antibody-immunocytochemistry-immunofluorescence-20122023122211..jpg)
![Western Blot: Centrin 1 Antibody [NBP2-14909] - Sample (30 ug of whole cell lysate) A: JurKat 12% SDS PAGE Centrin-1 antibody NBP2-14909 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-14909_rabbit-polyclonal-centrin-1-antibody-91020242063340.jpg)
![Western Blot: Tubby Antibody [NB100-1922] - Detection of tub-1 (GFP complex) using NB 100-1922. Tub-1 (nr2004) was used as a negative control.](http://images.novusbio.com/fullsize/Tubby-Antibody-Western-Blot-NB100-1922-img0004.jpg "Western Blot: Tubby Antibody [NB100-1922] - Detection of tub-1 (GFP complex) using NB 100-1922. Tub-1 (nr2004) was used as a negative control.")
![Western Blot: CtIP Antibody (19E8) [NBP1-41205] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CtIP antibody [19E8] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-41205_mouse-monoclonal-ctip-antibody-19e8-1610202419414519.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: CtIP Antibody (19E8) [NBP1-41205] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CtIP antibody [19E8] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>CtIP antibody [19E8] detects CtIP protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CtIP protein stained by CtIP antibody [19E8] (NBP1-41205) diluted at 1:100.Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.](http://images.novusbio.com/fullsize/nbp1-41205_mouse-monoclonal-ctip-antibody-19e8-immunocytochemistry-immunofluorescence-261120251974522.jpg)
![Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19935] - Mouse testis. PRMT5 stained by PRMT5 antibody [N1C1] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunohistochemistry-Paraffin-NBP2-19935-img0009.jpg "Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19935] - Mouse testis. PRMT5 stained by PRMT5 antibody [N1C1] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19935] - MCF7 xenograft, using PRMT5 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunohistochemistry-Paraffin-NBP2-19935-img0002.jpg)
![Western Blot: PRMT5 Antibody [NBP2-19935] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT5 antibody [N1C1] (NBP2-19935) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19935_rabbit-polyclonal-prmt5-antibody-91020242091465.jpg)
![PRMT5 antibody [N1C1] immunoprecipitates PRMT5 protein in IP experiments.IP samples: Raji whole cell extractA. 35 ug Raji whole cell extractB. Control with 4 ug of preimmune Rabbit IgGC. Immunoprecipitation of PRMT5 protein by 4 ug PRMT5 antibody [N1C1] (NBP2-19935)7.5 % SDS-PAGEThe immunoprecipitated PRMT5 protein was detected by PRMT5 antibody [N1C1] (NBP2-19935) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-19935_rabbit-polyclonal-prmt5-antibody-immunoprecipitation-261120251820238.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: CHAF1B Antibody [NB100-57523] - Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-CAF-1 p60 used at a dilution of 1:200 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-Immunohistochemistry-NB100-57523-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP1-33083] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33083-img0004.jpg "Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP1-33083] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP1-33083] - RFC4 antibody detects RFC4 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded MDA-MB-231xenograft. RFC4 antibody diluted at 1:250.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP1-33083-img0008.jpg)
![Western Blot: RFC4 Antibody [NBP1-33083] - Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NBP1-33083-img0006.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP1-33083] - Paraffin-embedded MDAMB-468 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP1-33083-img0005.jpg)
![Western Blot: RFC4 Antibody [NBP1-33083] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RFC4 antibody [N1C3] (NBP1-33083) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33083_rabbit-polyclonal-rfc4-antibody-910202420134095.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (12F3.3) [NB100-158] - 2mM hydroxyurea treated (right) or untreated (left) HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RPA32 protein stained by RPA32 antibody [12F3.3] Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/RPA2-Antibody-12F3-3-Immunocytochemistry-Immunofluorescence-NB100-158-img0003.jpg "<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RPA2 Antibody (12F3.3) [NB100-158] - 2mM hydroxyurea treated (right) or untreated (left) HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RPA32 protein stained by RPA32 antibody [12F3.3] Blue: Hoechst 33342 staining.")
![<b>Biological Strategies Validation. </b>Western Blot: RPA2 Antibody (12F3.3) [NB100-158] - Various whole cell extracts (30 ug) were separated by 12 % SDS-PAGE, and blotted with RPA32 antibody diluted by 1:500](http://images.novusbio.com/fullsize/RPA2-Antibody-12F3-3-Western-Blot-NB100-158-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: BIN1 Antibody [NBP2-15587] - BIN1 antibody detects BIN1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BIN1 stained by BIN1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15587-img0008.jpg "Immunocytochemistry/Immunofluorescence: BIN1 Antibody [NBP2-15587] - BIN1 antibody detects BIN1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BIN1 stained by BIN1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP2-15587] - BIN1 antibody detects BIN1 protein at cytoplasm in human papillary adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human papillary adenocarcinoma. BIN1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP2-15587-img0005.jpg)
![Western Blot: BIN1 Antibody [NBP2-15587] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BIN1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP2-15587-img0006.jpg)
![Western Blot: BIN1 Antibody [NBP2-15587] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BIN1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP2-15587-img0007.jpg)
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP2-15587] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP2-15587-img0003.jpg)
![Western Blot: SA1 Antibody [NB100-298] - Nuclear extract from approx. 500,000 HeLa cells. Antibody used at 0.5 ug/ml.](http://images.novusbio.com/fullsize/SA1-Antibody-Western-Blot-NB100-298-img0003.jpg "Western Blot: SA1 Antibody [NB100-298] - Nuclear extract from approx. 500,000 HeLa cells. Antibody used at 0.5 ug/ml.")
![Immunocytochemistry/Immunofluorescence: SA1 Antibody [NB100-298] - HeLa cells that were extracted for 5 min at 4C in 0.5% Triton in CSK buffer. Antibody used at 1 ug/ml.](http://images.novusbio.com/fullsize/SA1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-298-img0002.jpg)
![Western Blot: RB1 Antibody [NB100-56888] - Analysis of Retinoblastoma 1 in 30 ugs of Jurkat cell lysate using NB100-56888 at 1:1000 dilution.](http://images.novusbio.com/fullsize/RB1-Antibody-Western-Blot-NB100-56888-img0003.jpg "Western Blot: RB1 Antibody [NB100-56888] - Analysis of Retinoblastoma 1 in 30 ugs of Jurkat cell lysate using NB100-56888 at 1:1000 dilution.")
![Western Blot: EGFR Antibody (H9B4) [NBP2-34243] - Western Blot Analysis of A431 cell lysate using EGFR Mouse Monoclonal Antibody (H9B4).](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Western-Blot-NBP2-34243-img0006.jpg "Western Blot: EGFR Antibody (H9B4) [NBP2-34243] - Western Blot Analysis of A431 cell lysate using EGFR Mouse Monoclonal Antibody (H9B4).")
![Simple Western: EGFR Antibody (H9B4) [NBP2-34243] - Simple Western lane view shows a specific band for EGF R/ErbB1 in 0.2 mg/ml of A431 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Simple-Western-NBP2-34243-img0001.jpg)
![Simple Western: EGFR Antibody (H9B4) [NBP2-34243] - Simple Western lane view shows a specific band for EGF R/ErbB1 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Simple-Western-NBP2-34243-img0002.jpg)
![Simple Western: EGFR Antibody (H9B4) [NBP2-34243] - Electropherogram image of the corresponding Simple Western lane. EGF R/ErbB1 antibody was used at 10 ug/ml dilution of A431 and HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Simple-Western-NBP2-34243-img0004.jpg)
![Protein Array: EGFR Antibody (H9B4) [NBP2-34243] - Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Antibody (H9B4) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Protein-Array-NBP2-34243-img0005.jpg)