| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83350-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89524-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90925-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90013-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44025 | |||
| BSA Free | NBP2-52909-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90047-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-153SS | |||
| Azide and BSA Free | NBP2-80870 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56341SS | |||
![Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 in Western Blot and IP, using NB100-418. Samples: mock treated (control) or NF1-siRNA treated HeLa WCE. IPed NF was detected using an antibody from another vendor.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody NB100-418 used for IP at 6 ug per reaction. NF1 was also immunoprecipitated by another rabbit anti-NF1 antibody. For blotting immunoprecipitated NF1, NB100-418 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Immunoprecipitation-NB100-418-img0006.jpg)
![Neurofibromin loss sensitizes MEF cells to growth factor and matrix stimulation: (a) MEF cells isolated and immortalized from wild-type and knockout mice for Nf1 gene were analyzed by Western blotting with anti-neurofibromin (Nfn) antibodies; (b) focal adhesion kinase FAK binds to Nfn. FAK was immunoprecipitated (IP) from cell lysates of wild-type and knockout MEFs (Nf1+/+, Nf1−/−); following SDS-PAGE electrophoresis, co-immunoprecipitated Nfn were assayed by Western blotting analysis (IB) with anti-FAK and anti-Nfn antibodies. Total protein loaded on the IP is also shown as INPUT ((b), lower panel) and examined by anti-Nfn and anti-actin antibodies; (c) transient phosphorylation kinetics of MEFs stimulated with PDGF-BB (10 ng/mL), [38,39] for increasing time periods. Following SDS-PAGE, cell lysates were incubated with anti-phospho-Erk (1/2) (T202/Y204, T185/Y187) MAP kinase, phospho-FAK (Y397) and phospho-Src (Y416) antibodies and anti-GAPDH; (d,e) colony formation assay of MEFs fully embedded by low growth factor containing Matrigel in absence (upper panel) or presence (lower panel) of insoluble matrix proteins Collagen I/Fibronectin. Cells were seeded in the Matrigel and 24 h later treated or not with PDGF-BB ligand for 8 days (representative microscope pictures (10×)). Where indicated, MEF cells were additionally treated with FAK and MEK inhibitors named Y10 (0.62 μM) and Selumetinib (6.62 μM), respectively; the area of colonies was calculated in five fields for each well, and mean value and SD calculated and plotted in the right histograms. n = 8; * p < 0.05 **; p < 0.001 by Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34066061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-418_rabbit-polyclonal-neurofibromin-1-antibody-western-blot-132202618215117.jpg)
![Neurofibromin loss sensitizes MEF cells to growth factor and matrix stimulation: (a) MEF cells isolated and immortalized from wild-type and knockout mice for Nf1 gene were analyzed by Western blotting with anti-neurofibromin (Nfn) antibodies; (b) focal adhesion kinase FAK binds to Nfn. FAK was immunoprecipitated (IP) from cell lysates of wild-type and knockout MEFs (Nf1+/+, Nf1−/−); following SDS-PAGE electrophoresis, co-immunoprecipitated Nfn were assayed by Western blotting analysis (IB) with anti-FAK and anti-Nfn antibodies. Total protein loaded on the IP is also shown as INPUT ((b), lower panel) and examined by anti-Nfn and anti-actin antibodies; (c) transient phosphorylation kinetics of MEFs stimulated with PDGF-BB (10 ng/mL), [38,39] for increasing time periods. Following SDS-PAGE, cell lysates were incubated with anti-phospho-Erk (1/2) (T202/Y204, T185/Y187) MAP kinase, phospho-FAK (Y397) and phospho-Src (Y416) antibodies and anti-GAPDH; (d,e) colony formation assay of MEFs fully embedded by low growth factor containing Matrigel in absence (upper panel) or presence (lower panel) of insoluble matrix proteins Collagen I/Fibronectin. Cells were seeded in the Matrigel and 24 h later treated or not with PDGF-BB ligand for 8 days (representative microscope pictures (10×)). Where indicated, MEF cells were additionally treated with FAK and MEK inhibitors named Y10 (0.62 μM) and Selumetinib (6.62 μM), respectively; the area of colonies was calculated in five fields for each well, and mean value and SD calculated and plotted in the right histograms. n = 8; * p < 0.05 **; p < 0.001 by Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34066061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-92100_rabbit-polyclonal-mat2a-antibody-western-blot-13220261739820.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-83350] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83350-img0008.jpg "Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-83350] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody [NBP1-89524] - Staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89524-img0007.jpg "Immunocytochemistry/Immunofluorescence: RPA2 Antibody [NBP1-89524] - Staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Testis shows moderate nuclear positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0014.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Fallopian tube shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0011.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Liver shows very weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0012.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Testis cancer, carcinoma shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0013.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Tonsil shows moderate nuclear positivity in non-germinal and germinal center cells.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0015.jpg)
![Western Blot: DNA polymerase delta p50 Antibody [NBP1-90925] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-POLD2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/DNA-polymerase-delta-p50-Antibody-Western-Blot-NBP1-90925-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: DNA polymerase delta p50 Antibody [NBP1-90925] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-POLD2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Western Blot: DNA polymerase delta p50 Antibody [NBP1-90925] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/DNA-polymerase-delta-p50-Antibody-Western-Blot-NBP1-90925-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: DNA polymerase delta p50 Antibody [NBP1-90925] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DNA-polymerase-delta-p50-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90925-img0010.jpg)
![Immunohistochemistry-Paraffin: DNA polymerase delta p50 Antibody [NBP1-90925] - Staining of human Fallopian tube shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/DNA-polymerase-delta-p50-Antibody-Immunohistochemistry-Paraffin-NBP1-90925-img0018.jpg)
![Western Blot: DNA polymerase delta p50 Antibody [NBP1-90925] - Analysis in human cell line SK-BR-3.](http://images.novusbio.com/fullsize/DNA-polymerase-delta-p50-Antibody-Western-Blot-NBP1-90925-img0011.jpg)
![Immunohistochemistry-Paraffin: DNA polymerase delta p50 Antibody [NBP1-90925] - Staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/DNA-polymerase-delta-p50-Antibody-Immunohistochemistry-Paraffin-NBP1-90925-img0013.jpg)
![Immunohistochemistry-Paraffin: DNA polymerase delta p50 Antibody [NBP1-90925] - Staining of human pancreas shows very weak positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/DNA-polymerase-delta-p50-Antibody-Immunohistochemistry-Paraffin-NBP1-90925-img0014.jpg)
![Immunohistochemistry-Paraffin: DNA polymerase delta p50 Antibody [NBP1-90925] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/DNA-polymerase-delta-p50-Antibody-Immunohistochemistry-Paraffin-NBP1-90925-img0016.jpg)
![Immunohistochemistry-Paraffin: SNF5 Antibody [NBP1-90013] - Staining of human liver shows very weak positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunohistochemistry-Paraffin-NBP1-90013-img0013.jpg "Immunohistochemistry-Paraffin: SNF5 Antibody [NBP1-90013] - Staining of human liver shows very weak positivity in hepatocytes as expected.")
![Immunohistochemistry-Paraffin: SNF5 Antibody [NBP1-90013] - Staining of human cerebellum shows strong nuclear positivity in Purkinje cells and in cells in granular and molecular layer.](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunohistochemistry-Paraffin-NBP1-90013-img0011.jpg)
![Immunohistochemistry-Paraffin: SNF5 Antibody [NBP1-90013] - Staining of human duodenum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunohistochemistry-Paraffin-NBP1-90013-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: SNF5 Antibody [NBP1-90013] - Analysis using Anti-SMARCB1 antibody NBP1-90013 (A) shows similar pattern to independent antibody NBP1-90014 (B).](http://images.novusbio.com/fullsize/SNF5-Antibody-Western-Blot-NBP1-90013-img0009.jpg)
![Western Blot: CTCF Antibody (CL0304) [NBP2-52909] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CTCF antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Western-Blot-NBP2-52909-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CTCF Antibody (CL0304) [NBP2-52909] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CTCF antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CTCF Antibody (CL0304) [NBP2-52909] - Staining of MCF7 cells using the Anti-CTCF monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Immunocytochemistry-Immunofluorescence-NBP2-52909-img0011.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909] - Staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Immunohistochemistry-Paraffin-NBP2-52909-img0009.jpg)
![Western Blot: CTCF Antibody (CL0304) [NBP2-52909] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Western-Blot-NBP2-52909-img0004.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular and stromal cells.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Immunohistochemistry-Paraffin-NBP2-52909-img0006.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909] - Staining of human kidney shows moderate to strong nuclear positivity in cells in tubules and glomeruli.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Immunohistochemistry-Paraffin-NBP2-52909-img0007.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Immunohistochemistry-Paraffin-NBP2-52909-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: NPM1 Antibody [NBP1-90047] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NPM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90047-img0007.jpg "Immunocytochemistry/Immunofluorescence: NPM1 Antibody [NBP1-90047] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: NPM1 Antibody [NBP1-90047] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NPM1-Antibody-Immunohistochemistry-Paraffin-NBP1-90047-img0010.jpg)
![Immunohistochemistry-Paraffin: NPM1 Antibody [NBP1-90047] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/NPM1-Antibody-Immunohistochemistry-Paraffin-NBP1-90047-img0008.jpg)
![Immunohistochemistry-Paraffin: NPM1 Antibody [NBP1-90047] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/NPM1-Antibody-Immunohistochemistry-Paraffin-NBP1-90047-img0009.jpg)
![Immunohistochemistry-Paraffin: NPM1 Antibody [NBP1-90047] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/NPM1-Antibody-Immunohistochemistry-Paraffin-NBP1-90047-img0011.jpg)
![Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Western-Blot-NB300-153-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human brain using Neurofibromin 1 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of Neurofibromin 1was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Immunohistochemistry-Paraffin-NB300-153-img0005.jpg)
![Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw 264.7 whole cell lysate/extract. G. 30 ug C2Cl2 whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000](http://images.novusbio.com/fullsize/SET-Antibody-Western-Blot-NBP1-33713-img0008.jpg "Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw 264.7 whole cell lysate/extract. G. 30 ug C2Cl2 whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000")
![Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by Western blot analysis. A. 30 ug A431 whole cell lysate/extract whole cell lysate/extract. B. 30 ug H1299 whole cell lysate/extract. C. 30 ug HeLa whole cell lysate/extract. D. 30 ug HepG2 whole cell lysate/extract. E. 30 ug Molt-4 whole cell lysate/extract. F. 30 ug Raji whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000 SET antibody detects SET protein by Western blot analysis.Observed SET protein is larger than the predicted M.W., possibly due to post-translational](http://images.novusbio.com/fullsize/SET-Antibody-Western-Blot-NBP1-33713-img0004.jpg)
![Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/SET-Antibody-Western-Blot-NBP1-33713-img0005.jpg)
![Immunoprecipitation: SET Antibody [NBP1-33713] - SET antibody immunoprecipitates SET protein in IP experiments. IP samples: HeLa whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of SET protein by 4 ug SET antibody. 10 % SDS-PAGE. The immunoprecipitated SET protein was detected by SET antibody diluted at 1:500.](http://images.novusbio.com/fullsize/SET-Antibody-Immunoprecipitation-NBP1-33713-img0006.jpg)
![Western Blot: SET Antibody [NBP1-33713] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody (NBP1-33713) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33713_rabbit-polyclonal-set-antibody-910202420115093.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SET Antibody [NBP1-33713] - Non-transfected (–) and transfected (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp1-33713_rabbit-polyclonal-set-antibody-1610202419414526.jpg)
![SET antibody detects SET protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SET protein stained by SET antibody (NBP1-33713) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-33713_rabbit-polyclonal-set-antibody-immunocytochemistry-immunofluorescence-2611202518431924.jpg)
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NB100-56341] - Analysis of HDAC-4 in Jurkat cell lysate with this antibody.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-Western-Blot-NB100-56341-img0003.jpg "Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NB100-56341] - Analysis of HDAC-4 in Jurkat cell lysate with this antibody.")
![Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: LNCaP prostate cancer cells. Load: 50 ug per lane.Primary antibody: HDAC1 antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: 55kDa for HDAC1.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-78101-img0012.jpg "Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: LNCaP prostate cancer cells. Load: 50 ug per lane.Primary antibody: HDAC1 antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: 55kDa for HDAC1.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-78101] - Immunofluorescence Microscopy of HDAC1 antibody. Tissue: A431 cells. Fixation: methanol. Antigen retrieval: blocked with normal goat serum.Primary antibody: HDAC1 antibody at 4 ug/mL for 1 h at RT.Secondary antibody: rabbit secondary antibody at 0.2 ug/mL for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 as green fluorescent signal. A-tubulin monoclonal antibody detected with ATTO 425 (colored RED). 2-color STED image, Leica Microsystems.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78101-img0010.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-78101] - Immunohistochemistry of HDAC1 antibody. Tissue: human lung tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: HDAC1 antibody at 10 ug/mL for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 as brown color indicates presence of protein, blue color shows cell nuclei.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-NBP1-78101-img0014.jpg)
![Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: 293 whole cell lysate. Load: 35 ug per lane.Primary antibody: HDAC1 antibody at 1:3,500 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: ~65 kDa corresponding to human HDAC1. Other band(s): none.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-78101-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-78101] - Rabbit IgG 611-151-122 (shown in red) for to detect HDAC-1 by immunofluorescence. Anti Keratin monoclonal antibody was used with Dylight 488 goat anti mouse (shown in green) to detect Keratin](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78101-img0004.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-78101] - Immunohistochemistry of HDAC1 antibody. Tissue: human prostate cancer tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: HDAC1 antibody at 1:500 for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 precipitated purple with blue counterstain.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-NBP1-78101-img0013.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - GSPT1 antibody [HL1346] detects GSPT1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. GSPT1 stained by GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25493_rabbit-gspt1-mab-hl1346-azide-and-bsa-free-2120249355541.jpg "Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - GSPT1 antibody [HL1346] detects GSPT1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. GSPT1 stained by GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - GSPT1 antibody [HL1346] detects GSPT1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. GSPT1 stained by GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25493_rabbit-gspt1-mab-hl1346-azide-and-bsa-free-212024941458.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - GSPT1 antibody [HL1346] detects GSPT1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. GSPT1 stained by GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25493_rabbit-gspt1-mab-hl1346-azide-and-bsa-free-2120249383130.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - GSPT1 antibody [HL1346] detects GSPT1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. GSPT1 stained by GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25493_rabbit-gspt1-mab-hl1346-azide-and-bsa-free-2120249355586.jpg)
![Immunocytochemistry/Immunofluorescence: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - GSPT1 antibody [HL1346] detects GSPT1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSPT1 stained by GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25493_rabbit-gspt1-mab-hl1346-azide-and-bsa-free-2120249351367.jpg)
![Western Blot: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25493_rabbit-gspt1-mab-hl1346-azide-and-bsa-free-2120249342767.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25493_rabbit-gspt1-mab-hl1346-azide-and-bsa-free-western-blot-141120251842300.jpg)