| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75477SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80807-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47861-0.1mg | |||
| IHC-Prediluted | NBP2-48319 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56649SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84827-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33066 | |||
| IHC-Prediluted | NBP2-44365 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-1920SS | |||
![Western Blot: AGO1/EIF2C1 Antibody [NB100-2817] - HEK293 overexpressing AGO1/EIF2C1 and probed with NB100-2817 (non-transfected HEK293 in lane B).](http://images.novusbio.com/fullsize/AGO1-EIF2C1-Antibody-Western-Blot-NB100-2817-img0002.jpg "Western Blot: AGO1/EIF2C1 Antibody [NB100-2817] - HEK293 overexpressing AGO1/EIF2C1 and probed with NB100-2817 (non-transfected HEK293 in lane B).")
![Immunohistochemistry-Paraffin: AGO1/EIF2C1 Antibody [NB100-2817] - Staining of paraffin embedded Human Placenta. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/AGO1-EIF2C1-Antibody-Immunohistochemistry-Paraffin-NB100-2817-img0004.jpg)
![Immunohistochemistry-Paraffin: AGO1/EIF2C1 Antibody [NB100-2817] - Staining of paraffin embedded Human Small Intestine. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/AGO1-EIF2C1-Antibody-Immunohistochemistry-Paraffin-NB100-2817-img0003.jpg)
![Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Immunoprecipitation of RbAp48 protein from HeLa whole cell extract using 5 ug of RbAp48 antibody [13D10]. Western blot analysis was performed using RbAp48 antibody [13D10]. EasyBlot HRP-conjugated anti mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-13D10-Immunoprecipitation-NBP1-41201-img0017.jpg "Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Immunoprecipitation of RbAp48 protein from HeLa whole cell extract using 5 ug of RbAp48 antibody [13D10]. Western blot analysis was performed using RbAp48 antibody [13D10]. EasyBlot HRP-conjugated anti mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] -SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [13D10] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-41201_mouse-monoclonal-rbbp4-rbap48-antibody-13d10-western-blot-196202494114..jpg)
![RbAp48 antibody [13D10] detects RbAp48 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RbAp48 protein stained by RbAp48 antibody [13D10] (NBP1-41201) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-41201_mouse-monoclonal-rbbp4-rbap48-antibody-13d10-immunocytochemistry-immunofluorescence-2611202518283514.jpg)
![Immunocytochemistry/Immunofluorescence: beta Amyloid [Pyro Glu3] Antibody [NBP1-44048] - Laser scanning confocal microscopy was carried out using human tissue sections from frontal cortex. Figure A shows an example section that was immunostained for the presence of pyrE3Abeta (green). Figure B shows that the immunogen peptide completely blocked the NBP1-44048 antibody.](http://images.novusbio.com/fullsize/beta-Amyloid-[Pyro-Glu3]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-44048-img0005.jpg "Immunocytochemistry/Immunofluorescence: beta Amyloid [Pyro Glu3] Antibody [NBP1-44048] - Laser scanning confocal microscopy was carried out using human tissue sections from frontal cortex. Figure A shows an example section that was immunostained for the presence of pyrE3Abeta (green). Figure B shows that the immunogen peptide completely blocked the NBP1-44048 antibody.")
![Western Blot: beta Amyloid [Pyro Glu3] Antibody [NBP1-44048] - Lane 1-10: beta Amyloid pyro E3 peptide. Load: 1 ug per lane 1-2, 0.2 ug per lane 3-4, 0.05 ug per lane 5-6, 0.01 ug per lane 7-8, 0.005 ug per lane 9-10. Primary antibody: Beta Amyloid pyro E3 antibody at 0.5 ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 86.9 kDa for beta Amyloid pyro E3 peptide. Cross reactivity with Beta Amyloid 3 peptide is >1000 fold below pyrE3 reactivity and contains no reactivity towards the Beta Amyloid 42 A beta peptide (data not shown).](http://images.novusbio.com/fullsize/beta-Amyloid-[Pyro-Glu3]-Antibody-Western-Blot-NBP1-44048-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: beta Amyloid [Pyro Glu3] Antibody [NBP1-44048] - Tissue: human brain section. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: beta Amyloid pyro E3 antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: beta Amyloid pyro E3 is nuclear and cytoplasmic. Staining: Top: Beta Amyloid pyro E3 as as green fluorescent signal, Beta Amyloid 3 as yellow signal; and Bottom: Beta Amyloid 3 as red signal with co-incubation of Beta Amyloid pyro E3 peptide.](http://images.novusbio.com/fullsize/beta-Amyloid-[Pyro-Glu3]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-44048-img0007.jpg)
![Western Blot: beta Amyloid [Pyro Glu3] Antibody [NBP1-44048] - Lane 1: beta Amyloid 3 peptide Lane 2: beta Amyloid pyro E3 peptide. Load: 1 ug per lane. Primary antibody: Beta Amyloid pyro E3 antibody at 2ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 86.9 kDa for beta Amyloid pyro E3 peptide.](http://images.novusbio.com/fullsize/beta-Amyloid-[Pyro-Glu3]-Antibody-Western-Blot-NBP1-44048-img0010.jpg)
![Western Blot: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP1-75477-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP1-75477] - Analysis of Jurkat cell lysate (30ug) using anti-Hsp27 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP1-75477-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP1-75477] - Hsp27 Antibody [NBP1-75477] - HSP27 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-75477-img0006.jpg)
![Immunohistochemistry: Hsp27 Antibody [NBP1-75477] - Staining of HSP27 in mouse heart using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunohistochemistry-NBP1-75477-img0007.jpg)
![Western Blot: Hsp27 Antibody [NBP1-75477] - Analysis of HSP27 in human skeletal muscle lysate.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP1-75477-img0008.jpg)
![Western Blot: Hornerin Antibody [NBP1-80807] - Hornerin expression in HCC cell lines. Western blot analysis of Hornerin expression levels in a panel of HCC cell lines. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4719-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hornerin-Antibody-Western-Blot-NBP1-80807-img0013.jpg "Western Blot: Hornerin Antibody [NBP1-80807] - Hornerin expression in HCC cell lines. Western blot analysis of Hornerin expression levels in a panel of HCC cell lines. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4719-5), licensed under a CC-BY license.")
![Immunohistochemistry: Hornerin Antibody [NBP1-80807] - Hornerin overpression in human HCC tumor tissues. Immunohistochemistry of Hornerin expression in hepatocellular carcinoma (HCC) tissues. Hornerin expression in the cytoplasm and membrane is scored as negative (a, e), weak (b, f), moderate (c, g), and strong (d, h). Original magnification, x 100 (a-d); x 400 (e-h) Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4719-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hornerin-Antibody-Immunohistochemistry-NBP1-80807-img0011.jpg)
![Immunohistochemistry-Paraffin: Hornerin Antibody [NBP1-80807] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Hornerin-Antibody-Immunohistochemistry-Paraffin-NBP1-80807-img0007.jpg)
![Immunohistochemistry-Paraffin: Hornerin Antibody [NBP1-80807] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Hornerin-Antibody-Immunohistochemistry-Paraffin-NBP1-80807-img0008.jpg)
![Immunohistochemistry-Paraffin: Hornerin Antibody [NBP1-80807] - Staining of human skin shows moderate to strong cytoplasmic granular positivity in the cornified layer.](http://images.novusbio.com/fullsize/Hornerin-Antibody-Immunohistochemistry-Paraffin-NBP1-80807-img0009.jpg)
![Immunohistochemistry-Paraffin: Hornerin Antibody [NBP1-80807] - Staining of human testis shows weak positivity in peritubular myoid cells.](http://images.novusbio.com/fullsize/Hornerin-Antibody-Immunohistochemistry-Paraffin-NBP1-80807-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Hornerin Antibody [NBP1-80807] - Hornerin expression in HCC cell lines. RNR shRNAs inhibited the expression of Hornerin in PLC/PRF/5 and QGY-7703 cells Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4719-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hornerin-Antibody-Knockdown-Validated-NBP1-80807-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis HeLa cells labeling Nucleolin with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is DAPI (Blue).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunocytochemistry-Immunofluorescence-NBP2-44612-img0008.jpg "Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis HeLa cells labeling Nucleolin with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is DAPI (Blue).")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) [NBP2-44612] - Human Tonsil stained with Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunohistochemistry-Paraffin-NBP2-44612-img0003.jpg)
![Flow Cytometry: Nucleolin Antibody (364-5) [NBP2-44612] - Flow Cytometry of Human Nucleolin Ag on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Nucleolin Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Flow-Cytometry-NBP2-44612-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunocytochemistry-Immunofluorescence-NBP2-44612-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunocytochemistry-Immunofluorescence-NBP2-44612-img0007.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) [NBP2-44612] - Human Skin stained with Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunohistochemistry-Paraffin-NBP2-44612-img0002.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) [NBP2-44612] - Formalin-fixed paraffin-embedded human Colon Carcinoma stained with AF488 conjugate of Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunohistochemistry-Paraffin-NBP2-44612-img0004.jpg)
![Flow Cytometry: Nucleolin Antibody (364-5) [NBP2-44612] - Human Nucleolin Ag on 293T Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Flow-Cytometry-NBP2-44612-img0001.jpg)
![Western Blot: Lamin A + C Antibody [NB100-56649] - Analysis of human Lamin A + C in 15 ug of HeLa cell lysate using NB100-56649 at 1:1000.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NB100-56649-img0003.jpg "Western Blot: Lamin A + C Antibody [NB100-56649] - Analysis of human Lamin A + C in 15 ug of HeLa cell lysate using NB100-56649 at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NB100-56649] - Lamin A/C antibody was tested in A431 cells at 1:100 against DyLight 488 (Green). Alpha tubulin and nuclei were counterstained against DyLight 568 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56649-img0005.jpg)
![Flow (Intracellular): Lamin A + C Antibody [NB100-56649] - Intracellular staining of HeLa cells (1 x 10^6 cells/mL) with Lamin A+C antibody (orange) stained at 1:1000. Detected with a GtxRb DyLight 488 secondary. Shown with the secondary only control (blue).](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Flow-Intracellular-NB100-56649-img0004.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human cerebral cortex, cervix, uterine, endometrium and gastrointestinal using Anti-ER alpha/NR3A1 antibody NBP1-84827 (A) shows similar protein distribution across tissues to independent antibody NBP1-84826 (B).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human cell line MCF7 shows localization to nucleus & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84827-img0006.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human uterine cervix shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0024.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0019.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0022.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human small intestine shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0023.jpg)
![Western Blot: ANP32B Antibody [NB100-1199] - 0.1 ug/ml) staining of Human Tonsil (A) and Rat (B) and Pig (C) Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-1199-img0002.jpg "Western Blot: ANP32B Antibody [NB100-1199] - 0.1 ug/ml) staining of Human Tonsil (A) and Rat (B) and Pig (C) Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: ANP32B Antibody [NB100-1199] - LPS/IFN gamma-stimulated neuron-glial co-cultures (Glia/PAL31+ neuron) showing expression of both iNOS and PAL31. Image collected and cropped by CiteAb from the following publication (jbiomedsci.com/content/21/1/60), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1199-img0006.jpg)
![Immunohistochemistry: ANP32B Antibody [NB100-1199] - (C) Representative micrograph showing colocalization of upregulated PAL31 (green) with microglia/macrophage (ED1 positive cells) (D) Representative micrograph showing colocalization of upregulated PAL31 with astroglia (GFAP positive cells). (E) High power image showing the colocalization of upregulated PAL31 with ED1 positive macrophage/microglia (arrowhead) (F) High power image demonstrating the colocalization of upregulated PAL31 with GFAP positive astroglia (arrow). Magnification: 200X (A-D) and 1000X (E&F). Image collected and cropped by CiteAb from the following publication (jbiomedsci.com/content/21/1/60), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Immunohistochemistry-NB100-1199-img0005.jpg)
![Western Blot: ANP32B Antibody [NB100-1199] - (0.1 ug/ml) staining of NIH3T3 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-1199-img0001.jpg)
![Immunohistochemistry-Paraffin: ANP32B Antibody [NB100-1199] - Staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Immunohistochemistry-Paraffin-NB100-1199-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ANP32B Antibody [NB100-1199] - Western blot analysis of pal31 siRNA-treated C6 showing knockdown of PAL31 expression by PAL31 silencer using 41.5 (lane1), 83 (lane2), and 166 (lane 3) picomole of pal31 siRNA or 332 picomole negative control. Actin works as a loading control. Image collected and cropped by CiteAb from the following publication (jbiomedsci.com/content/21/1/60), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Knockdown-Validated-NB100-1199-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: ANP32B Antibody [NB100-1199] - Upregulated PAL31 expression was observed not only in microglia/ macrophage but also in glial cells near the injury site. (A) Normal thoracic spinal cord section showing few ED-1 positive cells (red). (B) Normal thoracic spinal cord section showing GFAP positive cells (red). (C) Representative micrograph showing colocalization of upregulated PAL31 (green) with microglia/macrophage (ED1 positive cells) (D) Representative micrograph showing colocalization of upregulated PAL31 with astroglia (GFAP positive cells). (E) High power image showing the colocalization of upregulated PAL31 with ED1 positive macrophage/microglia (arrowhead) (F) High power image demonstrating the colocalization of upregulated PAL31 with GFAP positive astroglia (arrow). Magnification: 200X (A-D) & 1000X (E&F). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25034417), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1199_goat-polyclonal-anp32b-antibody-3102024168397.jpg)
![Immunocytochemistry/ Immunofluorescence: ANP32B Antibody [NB100-1199] - Overexpression of PAL31 in C6 did not affect the cell proliferative activity. (A) GFP/bright field-superimposed micrograph in GFP overexpressing cells showing > 50% transfective efficiency (B) GFP-PAL31/bright field-micrograph in PAL31 overexpressing C6 showing correct nuclear localization of GFP-tagged PAL31 & transfection effeciency. (C) Western blot analysis showing the expression of GFP (~27 kDa), GFP-tagged PAL31 (~58 kDa), PCNA (~34 kDa), endogenous PAL31 (~31 kDa) & Actin (~43 kDa) in GFP- or PAL31-C6 cultures. The level of PCNA, a protein expressing in nuclear during DNA synthesis, in cells did not alter after overexpression of GFP or GFP-tagged PAL31. Actin works as a loading control. (D) MTT assay in the transfected cells at 4 different time intervals (from Ctrl to 72 hours). Ctrl represents the cells after subculture & overnight incubation. The data in each time points were analyzed by two-way ANOVA & Bonferroni post hoc test. No significance, compared GFP & GFP tagged PAL31 groups. n = 3. Magnification 100X (A-B). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25034417), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1199_goat-polyclonal-anp32b-antibody-310202415525215.jpg)
![Western Blot: ANP32B Antibody [NB100-1199] - Effect of overexpression or knockdown of PAL31 in C6 on H2O2-induced toxicity. (A) Representative micrographs showing C6 overexpressing GFP or GFP-tagged PAL31 after being treated with H2O2 (0 ~ 1 mM) for 4 hours. (B) MTT assay in GFP- or GFP-PAL31-overexpressed C6 after H2O2 treatment showing significant difference (cytoprotective effect of PAL31) at 0.5 mM & 1 mM H2O2 treatment between C6/GFP & C6/PAL31 groups. The data in each dosage were analyzed by two-way ANOVA & Bonferroni post hoc test. *P 50% transfective efficiency (B) GFP-PAL31/bright field-micrograph in PAL31 overexpressing C6 showing correct nuclear localization of GFP-tagged PAL31 & transfection effeciency. (C) Western blot analysis showing the expression of GFP (~27 kDa), GFP-tagged PAL31 (~58 kDa), PCNA (~34 kDa), endogenous PAL31 (~31 kDa) & Actin (~43 kDa) in GFP- or PAL31-C6 cultures. The level of PCNA, a protein expressing in nuclear during DNA synthesis, in cells did not alter after overexpression of GFP or GFP-tagged PAL31. Actin works as a loading control. (D) MTT assay in the transfected cells at 4 different time intervals (from Ctrl to 72 hours). Ctrl represents the cells after subculture & overnight incubation. The data in each time points were analyzed by two-way ANOVA & Bonferroni post hoc test. No significance, compared GFP & GFP tagged PAL31 groups. n = 3. Magnification 100X (A-B). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25034417), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1199_goat-polyclonal-anp32b-antibody-31020241682198.jpg)
![Western Blot: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Western Blot Analysis of human MCF-7 cell lysate using ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Western-Blot-NBP2-29439-img0011.jpg "Western Blot: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Western Blot Analysis of human MCF-7 cell lysate using ER beta/NR3A2 Antibody (ERb455).")
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0010.jpg)
![Flow Cytometry: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Flow Cytometry of human ER beta on BT474 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled ER beta/NR3A2 Mouse Monoclonal Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Flow-Cytometry-NBP2-29439-img0012.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin paraffin breast cancer stained with Estrogen receptor beta Ab (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0003.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-paraffin ovarian cancer stained with Estrogen receptor beta Ab (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0004.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0008.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0009.jpg)
![Flow Cytometry: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Flow Cytometry for human ER-beta on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Flow-Cytometry-NBP2-29439-img0007.jpg)
![Western Blot: Nucleolin Antibody [NB100-1920] - Validation of putative ORF1p-FLAG interacting proteins: Western blot images of the pJM101/L1.3FLAG and pJM101/L1.3 immunoprecipitation (IP) reactions. The pCEP4 lanes denote whole cell lysates derived from HeLa cells transfected with an empty pCEP4 vector (~ 1.0% input). Nucleolin antibody NB100-1920 (aNCL) used to probe western blot is indicated to the left of the image. Immunoprecipitation reactions were conducted in either the absence (left) or presence (right) of RNaseA (10 ug/mL). The putative cellular functions of the ORF1p-FLAG interacting proteins are indicated on the right hand side of the blots. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1005121#sec002) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Western-Blot-NB100-1920-img0010.jpg "Western Blot: Nucleolin Antibody [NB100-1920] - Validation of putative ORF1p-FLAG interacting proteins: Western blot images of the pJM101/L1.3FLAG and pJM101/L1.3 immunoprecipitation (IP) reactions. The pCEP4 lanes denote whole cell lysates derived from HeLa cells transfected with an empty pCEP4 vector (~ 1.0% input). Nucleolin antibody NB100-1920 (aNCL) used to probe western blot is indicated to the left of the image. Immunoprecipitation reactions were conducted in either the absence (left) or presence (right) of RNaseA (10 ug/mL). The putative cellular functions of the ORF1p-FLAG interacting proteins are indicated on the right hand side of the blots. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1005121#sec002) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [NB100-1920] - Detection of Nucleolin (green) in Hela cells detected with a Dylight-488 conjugated secondary antibody. Nuclei are counter stained with Hoechst 33258.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1920-img0007.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody [NB100-1920] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human colon cancer using 1:750 conc. of Nucleolin antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining was observed in normal cells and nuclear staining was observed in cancer cells. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Immunohistochemistry-Paraffin-NB100-1920-img0009.jpg)
![Western Blot: Nucleolin Antibody [NB100-1920] - Analysis of Nucleolin in HeLa nuclear extracts.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Western-Blot-NB100-1920-img0006.jpg)
![Western Blot: IL-6 Antibody [NBP2-16957] - Human breast cancer cell line MDA-MB-231 was treated by Stat3 inhibitor and expression of IL-6 was detected by Western blot. Image from verified customer review.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP2-16957-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: IL-6 Antibody [NBP2-16957] - Human breast cancer cell line MDA-MB-231 was treated by Stat3 inhibitor and expression of IL-6 was detected by Western blot. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Western Blot: IL-6 Antibody [NBP2-16957] - Recombinant IL6 were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP2-16957-img0010.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody [NBP2-16957] - IL-6 antibody detects IL-6 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.IL-6 stained by IL-6 antibody (NBP2-16957) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16957_rabbit-polyclonal-il-6-antibody-91020242011469.jpg)
