| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1768SS | |||
| Azide and BSA Free | NBP2-80668 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43954 | |||
| BSA Free | NBP2-34495-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87321-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40823SS | |||
![Western Blot: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis using Chk1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Chk1 cDNA (2).](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Western-Blot-NB100-1768-img0003.jpg "Western Blot: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis using Chk1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Chk1 cDNA (2).")
![Immunocytochemistry/Immunofluorescence: Chk1 Antibody (2G1D5) [NB100-1768] - Chk1 antibody was tested at 1:10 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X.](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Immunocytochemistry-Immunofluorescence-NB100-1768-img0005.jpg)
![Immunohistochemistry-Paraffin: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis of FFPE human lung using Chk1 antibody (clone 2G1D5) at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic and nuclear staining of Chk1 was observed as this is a ubiquitous cell checkpoint protein. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Immunohistochemistry-Paraffin-NB100-1768-img0006.jpg)
![Western Blot: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis using anti-Chk1 mouse mAb against A431 (1), Hela (2), NIH/3T3 (3) and K562 (4) cell lysates.](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Western-Blot-NB100-1768-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 stained by p21 Cip1 antibody [1032] diluted at 1:500.Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-1032-Immunocytochemistry-Immunofluorescence-NBP2-43697-img0010.jpg "Immunocytochemistry/Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 stained by p21 Cip1 antibody [1032] diluted at 1:500.Blue: Hoechst 33342 staining. Scale bar= 10 um.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Analysis of HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr A. 30 ug HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr B. Control with 4 ug of preimmune Mouse IgG C. Immunoprecipitation of CDKN1A protein by 4 ug p21 Cip1 antibody [1032] 15 % SDS-PAGE The immunoprecipitated CDKN1A protein was detected by p21 Cip1 antibody [1032] diluted at 1:1000. [EasyBlot anti-mouse IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-1032-Immunoprecipitation-NBP2-43697-img0002.jpg)
![p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis.Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody diluted at 1:1000.Red: p21 Cip1 protein stained by p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-immunocytochemistry-immunofluorescence-26112025183418.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-western-blot-26112025194811.jpg)
![Untreated (-) and treated (+) HCT-116 whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-western-blot-261120251846105.jpg)
![p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.p21 Cip1 stained by p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-immunohistochemistry-paraffin-2611202518315421.jpg)
![p21 Cip1 antibody [GT1032] (NBP2-43697) detects p21 Cip1 by flow cytometry analysis. Sample: HepG2 cell. Black: Unlabelled sample was used as a control. Red: p21 Cip1 antibody [GT1032] (NBP2-43697) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-flow-cytometry-2611202518321914.jpg)
![Untreated (-) and treated (+) HCT 116 whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-western-blot-261120251836336.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining in human skin and skeletal muscle tissues. Corresponding p53 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining in human skin and skeletal muscle tissues. Corresponding p53 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-P53 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Western-Blot-NBP2-34495-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in U2OS cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0014.jpg)
![Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Lane 1: Marker [kDa] Lane 2: Human cell line U-251](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Western-Blot-NBP2-34495-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining of human cell line A431 showing cell cycle dependent nuclear (without nucleoli)Staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in MCF7 cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in U251 cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human colorectal cancer shows strong nuclear positivity in tumor cells, but not in normal mucosa.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0017.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human lung cancer (squamous cell carcinoma) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0018.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human skin shows moderate to strong nuclear positivity in a subset of epidermal cells.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0019.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0020.jpg)
![Simple Western: p53 Antibody (CL2199) [NBP2-34495] - Simple Western lane view shows a specific band for P53 in 0.2 mg/ml of Hek293T lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Simple-Western-NBP2-34495-img0009.jpg)
![Simple Western: p53 Antibody (CL2199) [NBP2-34495] - Electropherogram image(s) of corresponding Simple Western lane view. p53 antibody was used at 1:50 dilution on Hek293T lysate(s).](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Simple-Western-NBP2-34495-img0011.jpg)
![Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against human Bmi1 (AA: 1-326) recombinant protein. (Expected MW is 74 kDa)](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Western-Blot-NBP2-22204-img0007.jpg "Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against human Bmi1 (AA: 1-326) recombinant protein. (Expected MW is 74 kDa)")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of Hela cells using Bmi1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Immunocytochemistry-Immunofluorescence-NBP2-22204-img0002.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of paraffin-embedded rectum cancer tissues using Bmi1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Immunohistochemistry-Paraffin-NBP2-22204-img0004.jpg)
![Flow Cytometry: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of NIH/3T3 cells using Bmi1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Flow-Cytometry-NBP2-22204-img0001.jpg)
![Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Western-Blot-NBP2-22204-img0006.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of paraffin-embedded cervical cancer tissues using Bmi1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Immunohistochemistry-Paraffin-NBP2-22204-img0003.jpg)
![ELISA: BMI-1 Antibody (3E3) [NBP2-22204] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-ELISA-NBP2-22204-img0005.jpg)
![Western Blot: Xrn1 Antibody [NB500-191] - Detection of human and mouse XRN1 by western blot. Samples:Whole cell lysate (15 ug) from GaMG, HEK293T, RKO, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN1 antibody NB500-191 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Xrn1-Antibody-Western-Blot-NB500-191-img0010.jpg "Western Blot: Xrn1 Antibody [NB500-191] - Detection of human and mouse XRN1 by western blot. Samples:Whole cell lysate (15 ug) from GaMG, HEK293T, RKO, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN1 antibody NB500-191 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation: Xrn1 Antibody [NB500-191] - Detection of human XRN1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XRN1 antibody NB500-191 (lot NB500-191-4) used for IP at 6 ug per reaction. XRN1 was also immunoprecipitated by a previous lot of this antibody (lot NB500-191-3). For blotting immunoprecipitated XRN1, NB500-191 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Xrn1-Antibody-Immunoprecipitation-NB500-191-img0011.jpg)
![Western Blot: Lamin A + C Antibody (4L8Q0) [NBP3-15754]- Western blot analysis of extracts from normal (control) and Lamin A + C knockout (KO) HeLa cells, using Lamin A + C antibody (NBP3-15754) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4L8Q0-Knockout-Validated-NBP3-15754-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Lamin A + C Antibody (4L8Q0) [NBP3-15754]- Western blot analysis of extracts from normal (control) and Lamin A + C knockout (KO) HeLa cells, using Lamin A + C antibody (NBP3-15754) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunofluorescence analysis of U2OS cells using Lamin A + C antibody (NBP3-15754) at dilution of 1:100 - 1:1600. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4L8Q0-Immunocytochemistry-Immunofluorescence-NBP3-15754-img0007.jpg)
![Immunoprecipitation: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunoprecipitation analysis of 200ug extracts of K-562 cells using 3ug Lamin A + C antibody (NBP3-15754). Western blot was performed from the immunoprecipitate using Lamin A + C antibody (NBP3-15754) at a dilition of 1:20000.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4L8Q0-Immunoprecipitation-NBP3-15754-img0011.jpg)
![Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517311038.jpg)
![Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517333869.jpg)
![Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517315547.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Confocal imaging of U-2 OS cells using [KO Validated] Lamin A + C Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517315568.jpg)
![Immunoprecipitation: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunoprecipitation analysis of 300 ug extracts from PC-12 cells using 3 ug [KO Validated] Lamin A + C Rabbit mAb . Western blot was performed from the immunoprecipitate using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:100000.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517352763.jpg)
![Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517311069.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - The STORM super-resolution (SR) imaging of U-2 OS cells using [KO Validated] Lamin A + C Rabbit mAb at dilution of 1:200 with 3% paraformaldehyde (PFA) +0.1% glutaraldehyde (GA) fixation. The immunostaining was performed by Full Automatic Immunofluorescence Workflow System (Workflow Ultra300, Nano-Micro imaging, China). Image was performed with Single-Molecule Localization Super-Resolution Microscopy (STORM Ultra300, Nano-Micro imaging, China). We acknowledge Nano-Micro imaging Biotechnology Co., Ltd. in SR image processing and kindly providing this image.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517344285.jpg)
![Western Blot: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Western blot analysis of lysates from A549 cells using [KO Validated] Lamin A + C Rabbit mAb at 1:240000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517315542.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunofluorescence analysis of L929 cells using [KO Validated] Lamin A + C Rabbit mAb at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-5220251733550.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunofluorescence analysis of H9C2 cells using [KO Validated] Lamin A + C Rabbit mAb at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-5220251734531.jpg)
![Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517352736.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Confocal immunofluorescence analysis of HeLa cells using [KO Validated] Lamin A + C Rabbit mAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-5220251733511.jpg)
![Western Blot: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Western blot analysis of various lysates using [KO Validated] Lamin A + C Rabbit mAb at 1:300000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517344232.jpg)
![Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517361239.jpg)
![Western Blot: HMGA1 Antibody (5J0E6) [NBP3-16379] - Western blot analysis of extracts of various cell lines, using HMGA1 Rabbit mAb (NBP3-16379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HMGA1-Antibody-5J0E6-Western-Blot-NBP3-16379-img0005.jpg "Western Blot: HMGA1 Antibody (5J0E6) [NBP3-16379] - Western blot analysis of extracts of various cell lines, using HMGA1 Rabbit mAb (NBP3-16379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511383742.jpg)
![Chromatin Immunoprecipitation: HMGA1 Antibody (5J0E6) [NBP3-16379] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of HMGA1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2. The ChIP sequencing results indicate the enrichment pattern of HMGA1 across chromosome 12(upper panel) and the genomic region encompassing HNF1A, a representative gene enriched in HMGA1 (lower panel).](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-5220251141141.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511374182.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-5220251255541.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511402740.jpg)
![Western Blot: HMGA1 Antibody (5J0E6) [NBP3-16379] - Western blot analysis of lysates from Mouse testis, using HMGA1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-5220251215443.jpg)
![Chromatin Immunoprecipitation: HMGA1 Antibody (5J0E6) [NBP3-16379] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of HMGA1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2. The ChIP sequencing results indicate the enrichment pattern of HMGA1 in the representative genomic region surrounding HNF1A gene.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511374168.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511374132.jpg)
![Chromatin Immunoprecipitation: HMGA1 Antibody (5J0E6) [NBP3-16379] - Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using HMGA1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-5220251215418.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511361253.jpg)
![Immunocytochemistry/ Immunofluorescence: HMGA1 Antibody (5J0E6) [NBP3-16379] - Confocal imaging of U-2 OS cells using HMGA1 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511411472.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-5220251215471.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511374188.jpg)
![Western Blot: p21/CIP1/CDKN1A Antibody (1L4J1) [NBP3-15661] - Analysis of extracts of various cell lines, using p21/CIP1/CDKN1A antibody (NBP3-15661) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-1L4J1-Western-Blot-NBP3-15661-img0010.jpg "Western Blot: p21/CIP1/CDKN1A Antibody (1L4J1) [NBP3-15661] - Analysis of extracts of various cell lines, using p21/CIP1/CDKN1A antibody (NBP3-15661) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![<b>Genetic Strategies Validation. </b>Western Blot: p21/CIP1/CDKN1A Antibody (1L4J1) [NBP3-15661] -analysis of lysates from wild type (WT) and CDKN1A/p21 knockout (KO) 293T cells, using [KO Validated] CDKN1A/p21 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-western-blot-305202492857..jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-52202517344271.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-5220251730319.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-5220251731552.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-52202517361213.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-52202517311090.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-52202517352775.jpg)
![Western Blot: Lamin A + C Antibody [NBP2-19323] - Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NBP2-19323-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Lamin A + C Antibody [NBP2-19323] - Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded human breast carcinoma. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0012.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Lamin A/C antibody detects LMNA protein at membrane on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Lamin A/C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0002.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded mouse prostate. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0007.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded mouse fore brain. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0008.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded mouse skin. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0009.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded human endometrial carcinoma. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0011.jpg)
![Western Blot: BMI-1 Antibody [NBP1-87321] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-87321-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: BMI-1 Antibody [NBP1-87321] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-87321] - Staining of human cerebral cortex shows moderate nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87321-img0012.jpg)
![Western Blot: BMI-1 Antibody [NBP1-87321] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-87321-img0007.jpg)
![Western Blot: BMI-1 Antibody [NBP1-87321] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-87321-img0008.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-87321] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87321-img0010.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-87321] - Staining of human prostate shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87321-img0011.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-87321] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87321-img0013.jpg)
![Western Blot: BMI-1 Antibody [NBP2-24693] - Analysis of BMI1 in skeletal muscle lysate. Lane 1 shows pre-immune sera. Lanes 2, 3 and 4 show this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP2-24693-img0006.jpg "Western Blot: BMI-1 Antibody [NBP2-24693] - Analysis of BMI1 in skeletal muscle lysate. Lane 1 shows pre-immune sera. Lanes 2, 3 and 4 show this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody [NBP2-24693] - Immunofluorescence of BMI1 in human oral squamous cell carcinoma (UPCI:SCC029B) cells. These cells are part of a large collection of HNSCC (head and neck SCC) lines established by the Gollin lab (White et al., 2007). The BMI1 antibody was used at 5 ug/ml. Staining is primarily nuclear, some cytoplasmic staining is also observed. Data courtesy of Hatem Kaseb, MD and Susanne M. Gollin, PhD.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24693-img0004.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP2-24693] - Analysis of BMI1 in formalin-fixed, paraffin-embedded human cervix tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP2-24693-img0005.jpg)
![Western Blot: Bmi1 Antibody [NB110-40823] - Detection of Bmi1 protein in skeletal muscle](http://images.novusbio.com/fullsize/Bmi1-Antibody-Western-Blot-NB110-40823-img0002.jpg "Western Blot: Bmi1 Antibody [NB110-40823] - Detection of Bmi1 protein in skeletal muscle")
![Immunocytochemistry/Immunofluorescence: Bmi1 Antibody [NB110-40823] - BMI1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Bmi1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40823-img0004.jpg)