| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-68716-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08966 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-30536-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76698-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92963-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61694-0.025ml | |||
![Immunohistochemistry-Paraffin: EEF1G Antibody [NBP2-68716] - Staining of human testis.](http://images.novusbio.com/fullsize/EEF1G-Antibody-Immunohistochemistry-Paraffin-NBP2-68716-img0005.jpg "Immunohistochemistry-Paraffin: EEF1G Antibody [NBP2-68716] - Staining of human testis.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: EEF1G Antibody [NBP2-68716] - Staining of human cerebellum, cerebral cortex, lymph node and testis using Anti-EEF1G antibody NBP2-68716 (A) shows similar protein distribution across tissues to independent antibody NBP1-91862 (B).](http://images.novusbio.com/fullsize/EEF1G-Antibody-Immunohistochemistry-Paraffin-NBP2-68716-img0002.jpg)
![Immunohistochemistry-Paraffin: EEF1G Antibody [NBP2-68716] - Staining of human cerebellum.](http://images.novusbio.com/fullsize/EEF1G-Antibody-Immunohistochemistry-Paraffin-NBP2-68716-img0003.jpg)
![Immunohistochemistry-Paraffin: EEF1G Antibody [NBP2-68716] - Staining of human lymph node.](http://images.novusbio.com/fullsize/EEF1G-Antibody-Immunohistochemistry-Paraffin-NBP2-68716-img0004.jpg)
![Immunohistochemistry-Paraffin: EEF1G Antibody [NBP2-68716] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/EEF1G-Antibody-Immunohistochemistry-Paraffin-NBP2-68716-img0006.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) [NBP3-07221] - FFPE human kidney. PBS instead of primary antibody. Secondary antibody only negative control. Hematoxylin counterstain.](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-3477R-Immunohistochemistry-Paraffin-NBP3-07221-img0003.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) [NBP3-07221] - FFPE human kidney. PBS instead of primary antibody. Secondary antibody only negative control. Hematoxylin counterstain.")
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) [NBP3-07221] - FFPE human kidney sections. Wilm's Tumor 1 labeled using WT1 (WT1/3477R) (1?g/ml). HIER: Tris/EDTA (pH9.0). HRP-Polymer. Hematoxylin counterstain.](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-3477R-Immunohistochemistry-Paraffin-NBP3-07221-img0001.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) [NBP3-07221] - FFPE human kidney. Secondary antibody only negative control. Hematoxylin counterstain.](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-3477R-Immunohistochemistry-Paraffin-NBP3-07221-img0002.jpg)
![Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP2-39012] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/Prostatic-Acid-Phosphatase-ACPP-Antibody-Western-Blot-NBP2-39012-img0002.jpg "Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP2-39012] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG")
![Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP2-39012] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize/Prostatic-Acid-Phosphatase-ACPP-Antibody-Immunohistochemistry-Paraffin-NBP2-39012-img0004.jpg)
![Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP2-39012] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Prostatic-Acid-Phosphatase-ACPP-Antibody-Immunohistochemistry-Paraffin-NBP2-39012-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP2-39012] - Staining in human prostate and endometrium tissues using anti-ACPP antibody. Corresponding ACPP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Prostatic-Acid-Phosphatase-ACPP-Antibody-Immunohistochemistry-Paraffin-NBP2-39012-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: B7-H4 Antibody [NBP2-30536] - Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane & cell junctions.](http://images.novusbio.com/fullsize/B7-H4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30536-img0002.jpg "Immunocytochemistry/Immunofluorescence: B7-H4 Antibody [NBP2-30536] - Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane & cell junctions.")
![Immunohistochemistry-Paraffin: B7-H4 Antibody [NBP2-30536] - Staining of human breast shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/B7-H4-Antibody-Immunohistochemistry-Paraffin-NBP2-30536-img0003.jpg)
![Western Blot: IL-27 Antibody [NBP1-76698] - Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17(IL-17A+IL-17B) protein per lane. Antibodies: IL-27 NBP1-76698, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL.](http://images.novusbio.com/fullsize/IL-27-Antibody-Western-Blot-NBP1-76698-img0005.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: IL-27 Antibody [NBP1-76698] - Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17(IL-17A+IL-17B) protein per lane. Antibodies: IL-27 NBP1-76698, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL.")
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: IL-27 Antibody [NBP1-76698] - Analysis of IL-27 in A-20 cell lysate with IL-27 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/IL-27-Antibody-Western-Blot-NBP1-76698-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: IL-27 Antibody [NBP1-76698] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: IL-27 NBP1-76698 (4 ug/mL), IL-27 NBP1-76412 (4 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-27-Antibody-Western-Blot-NBP1-76698-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-27 Antibody - BSA Free [NBP1-76698] - Validation of IL-27 in Mouse EL4 Cells. Immunocytochemical analysis of Mouse EL4 cells using anti-IL-27 antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76698_rabbit-polyclonal-il-27-antibody-2711202420212370.jpg)
![Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-51946] - Staining of Human Prostate lysate (35 ug protein in RIPA buffer) with antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Prostatic-Acid-Phosphatase-ACPP-Antibody-Western-Blot-NBP1-51946-img0006.jpg "Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-51946] - Staining of Human Prostate lysate (35 ug protein in RIPA buffer) with antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-51946] - Staining of paraffin embedded Human Prostate with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Prostatic-Acid-Phosphatase-ACPP-Antibody-Immunohistochemistry-Paraffin-NBP1-51946-img0005.jpg)
![Western Blot: ARHGEF1 Antibody [NBP2-92963] - Analysis of extracts of various cell lines, using ARHGEF1 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/ARHGEF1-Antibody-Western-Blot-NBP2-92963-img0003.jpg "Western Blot: ARHGEF1 Antibody [NBP2-92963] - Analysis of extracts of various cell lines, using ARHGEF1 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunohistochemistry-Paraffin: ARHGEF1 Antibody [NBP2-92963] - Human tonsil using ARHGEF1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/ARHGEF1-Antibody-Immunohistochemistry-Paraffin-NBP2-92963-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ARHGEF1 Antibody [NBP2-92963] - Analysis of extracts from normal (control) and ARHGEF1 knockout (KO) 293T cells, using ARHGEF1 at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in](http://images.novusbio.com/fullsize/ARHGEF1-Antibody-Knockout-Validated-NBP2-92963-img0002.jpg)
![Western Blot: Aurora A Antibody (4H5A8) [NBP2-61694] - Analysis using AURKA mAb against HEK293 (1) and AURKA (AA: 268-404)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-4H5A8-Western-Blot-NBP2-61694-img0003.jpg "Western Blot: Aurora A Antibody (4H5A8) [NBP2-61694] - Analysis using AURKA mAb against HEK293 (1) and AURKA (AA: 268-404)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: Aurora A Antibody (4H5A8) [NBP2-61694] - Analysis using AURKA mAb against human AURKA (AA: 268-404) recombinant protein. (Expected MW is 41.5 kDa)](http://images.novusbio.com/fullsize/Aurora-A-Antibody-4H5A8-Western-Blot-NBP2-61694-img0002.jpg)
![Western Blot: Aurora A Antibody (4H5A8) [NBP2-61694] - Analysis using AURKA mouse mAb against HEK293 (1), PC-12 (2), and HT-29 (3) cell lysate.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-4H5A8-Western-Blot-NBP2-61694-img0004.jpg)
![ELISA: Aurora A Antibody (4H5A8) [NBP2-61694] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Aurora-A-Antibody-4H5A8-ELISA-NBP2-61694-img0001.jpg)
![Western Blot: B7-H2/ICOSLG Antibody (OTI2C5) - Azide and BSA Free [NBP2-71735] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICOSLG.](http://images.novusbio.com/fullsize/B7-H2-ICOSLG-Antibody-OTI2C5-Azide-and-BSA-Free-Western-Blot-NBP2-71735-img0001.jpg "Western Blot: B7-H2/ICOSLG Antibody (OTI2C5) - Azide and BSA Free [NBP2-71735] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICOSLG.")
![Immunohistochemistry: B7-H2/ICOSLG Antibody (OTI2C5) - Azide and BSA Free [NBP2-71735] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/B7-H2-ICOSLG-Antibody-OTI2C5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71735-img0003.jpg)
![Immunohistochemistry: B7-H2/ICOSLG Antibody (OTI2C5) - Azide and BSA Free [NBP2-71735] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/B7-H2-ICOSLG-Antibody-OTI2C5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71735-img0002.jpg)
![Immunohistochemistry: B7-H2/ICOSLG Antibody (OTI2C5) - Azide and BSA Free [NBP2-71735] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/B7-H2-ICOSLG-Antibody-OTI2C5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71735-img0004.jpg)
![Western Blot: SRPK1 Antibody [NBP1-30108] - Detection of human and mouse SRPK1 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SRPK1 antibody NBP1-30108 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SRPK1 was also immunoprecipitated by another rabbit anti-SRPK1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/SRPK1-Antibody-Western-Blot-NBP1-30108-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: SRPK1 Antibody [NBP1-30108] - Detection of human and mouse SRPK1 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SRPK1 antibody NBP1-30108 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SRPK1 was also immunoprecipitated by another rabbit anti-SRPK1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SRPK1 Antibody [NBP1-30108] - Section of human testicular seminoma. Antibody: Affinity purified rabbit anti- SRPK1 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SRPK1-Antibody-Immunohistochemistry-NBP1-30108-img0002.jpg)
![Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-32185] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32185 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Western-Blot-NBP2-32185-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-32185] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32185 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Tryptophanyl tRNA synthetase Antibody [NBP2-32185] - Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- WARS used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Immunohistochemistry-NBP2-32185-img0003.jpg)
![Immunoprecipitation: Tryptophanyl tRNA synthetase Antibody [NBP2-32185] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32185 used for IP at 6 ug per reaction. For blotting immunoprecipitated Tryptophanyl tRNA synthetase, NBP2-32185 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Immunoprecipitation-NBP2-32185-img0002.jpg)