| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84342-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61780-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38231-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58867SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82785-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58043-25ul | |||
![Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human colon, kidney, liver and testis using Anti-PBK antibody NBP1-84342 (A) shows similar protein distribution across tissues to independent antibody NBP2-58043 (B).](http://images.novusbio.com/fullsize/PBK-Antibody-Immunohistochemistry-Paraffin-NBP1-84342-img0013.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human colon, kidney, liver and testis using Anti-PBK antibody NBP1-84342 (A) shows similar protein distribution across tissues to independent antibody NBP2-58043 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PBK Antibody [NBP1-84342] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84342-img0012.jpg)
![Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human testis.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunohistochemistry-Paraffin-NBP1-84342-img0017.jpg)
![Immunohistochemistry: PBK Antibody [NBP1-84342] - Staining of mouse brain shows positivity in rostral migratory stream.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84342-img0006.jpg)
![Immunohistochemistry: PBK Antibody [NBP1-84342] - Staining of mouse medulla shows positivity in a subset of cells.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84342-img0007.jpg)
![Immunohistochemistry: PBK Antibody [NBP1-84342] - Staining of mouse subventricular zone shows immunoreactivity in some cells in the rostral migratory stream.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84342-img0009.jpg)
![Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human colon.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunohistochemistry-Paraffin-NBP1-84342-img0014.jpg)
![Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human kidney.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunohistochemistry-Paraffin-NBP1-84342-img0015.jpg)
![Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human liver.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunohistochemistry-Paraffin-NBP1-84342-img0016.jpg)
![Western Blot: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Western-Blot-NBP3-13648-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Rat brain. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - AIF-1/Iba1 antibody [HL22] detects AIF-1/Iba1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse spinal cord. Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:200. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Frozen-NBP3-13648-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Mouse hippocampus. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Rat cerebellum. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0003.jpg)
![Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - AIF-1/Iba1 antibody [HL22] detects AIF-1/Iba1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse brain. Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:100. Red: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22-RT] diluted at 1:100. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Frozen-NBP3-13648-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody [HL22] diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-immunocytochemistry-immunofluorescence-244202411033..jpg)
![Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Iba1 stained by Iba1 antibody [HL22] (NBP3-13648) conjugated with DyLight488 diluted at 1:200.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-immunohistochemistry-paraffin-261120251850420.jpg)
![Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-western-blot-2611202518454020.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mAb against HEK293 (1) and YWHAB (AA: 1-246)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Western-Blot-NBP2-61780-img0009.jpg "Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mAb against HEK293 (1) and YWHAB (AA: 1-246)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of HepG2 cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunocytochemistry-Immunofluorescence-NBP2-61780-img0005.jpg)
![Immunohistochemistry: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of paraffin-embedded endometrial cancer tissues using YWHAB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunohistochemistry-NBP2-61780-img0007.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mAb against human YWHAB (AA: 1-246) recombinant protein. (Expected MW is 54 kDa)](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Western-Blot-NBP2-61780-img0008.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mouse mAb against Hela (1), NIH/3T3 (2), C6 (3), A431 (4), K562 (5), PC-12 (6), and U937 (7) cell lysate.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Western-Blot-NBP2-61780-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of GC-7901 cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunocytochemistry-Immunofluorescence-NBP2-61780-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of Hela cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunocytochemistry-Immunofluorescence-NBP2-61780-img0004.jpg)
![Immunohistochemistry: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of paraffin-embedded rectum cancer tissues using YWHAB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunohistochemistry-NBP2-61780-img0006.jpg)
![ELISA: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-ELISA-NBP2-61780-img0001.jpg)
![ELISA: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of Hela cells using YWHAB mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-ELISA-NBP2-61780-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Fumarase Antibody [NBP1-89814] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89814-img0015.jpg "Immunocytochemistry/Immunofluorescence: Fumarase Antibody [NBP1-89814] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.")
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse hippocampus shows neuronal immunoreactivity in the CA3 layer.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342022.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse globus pallidus shows immunoreactivity in neurons.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-1752023133407.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse medulla shows positivity in the neurons of the facial nucleus.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342036.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse olfactory bulb shows neuronal positivity in the anterior olfactory nucleus.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-17520231334012.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342060.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-17520231334201.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-1752023133402.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342070.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Fumarase Antibody [NBP1-89814] - Analysis using Anti-FH antibody NBP1-89814 (A) shows similar pattern to independent antibody NBP1-89813 (B).](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-89814-img0018.jpg)
![Western Blot: Coronin-1a Antibody (4G10) [H00011151-M01] - CORO1A monoclonal antibody (M01), clone 4G10 Analysis of CORO1A expression in K-562.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-4G10-Western-Blot-H00011151-M01-img0007.jpg "Western Blot: Coronin-1a Antibody (4G10) [H00011151-M01] - CORO1A monoclonal antibody (M01), clone 4G10 Analysis of CORO1A expression in K-562.")
![Immunohistochemistry-Paraffin: Coronin-1a Antibody (4G10) [H00011151-M01] - Analysis of monoclonal antibody to CORO1A on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-4G10-Immunohistochemistry-Paraffin-H00011151-M01-img0005.jpg)
![Sandwich ELISA: Coronin-1a Antibody (4G10) [H00011151-M01] - Detection limit for recombinant GST tagged CORO1A is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-4G10-Sandwich-ELISA-H00011151-M01-img0006.jpg)
![Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Western-Blot-NBP2-22527-img0006.jpg "Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.")
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunocytochemistry-Immunofluorescence-NBP2-22527-img0003.jpg)
![Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human colon carcinoma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunohistochemistry-Paraffin-NBP2-22527-img0005.jpg)
![Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human kidney tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunohistochemistry-Paraffin-NBP2-22527-img0004.jpg)
![Immunoprecipitation: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 whole cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample BUffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunoprecipitation-NBP2-22527-img0002.jpg)
![Western Blot: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Whole cell lysate (10 µg) from HEK293T, HDLM-2, Jurkat,MJ, and HeLa cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD86 recombinant monoclonalantibody [BLR030F] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with anexposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-western-blot-1311202411142..png "Western Blot: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Whole cell lysate (10 µg) from HEK293T, HDLM-2, Jurkat,MJ, and HeLa cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD86 recombinant monoclonalantibody [BLR030F] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with anexposure time of 10 seconds.")
![Flow Cytometry: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Antibody: Rabbit anti-CD86 recombinantmonoclonal [BLR030F] or isotype control(unshaded). Secondary: DyLight® 488-conjugated goatanti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-flow-cytometry-1311202411333..png)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] -FFPE section of tonsil. Antibody: Rabbit anti-CD86recombinant monoclonal antibody [BLR030F]. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunohistochemistry-paraffin-131120241151..png)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] -Section of HDLM-2 cells. Antibody: Rabbit anti-CD86recombinant monoclonal antibody [BLR030F]. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunocytochemistry-immunofluorescence-1311202411712..png)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: NBP2-76417 Lot 4 used for immunoprecipitation at 6 ul per reaction. B7-2/CD86 was also immunoprecipitated by a previous lot of this antibody (NBP2-76417 Lot 1) and a second antibody, NBP2-76416, against a different epitope of B7-2/CD86 (BLR029F). For blotting immunoprecipitated CD86, NBP2-76417 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunoprecipitation-1312025145029..jpg)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Detection of human CD86 by WB of immunoprecipitates from HDML-2 lysate. Antibodies: Rabbit anti-CD86 recombinant monoclonal [BLR030F] (NBP2-76417) and rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416).](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunoprecipitation-131202515750..jpg)
![Western Blot: Flotillin-1 Antibody [NBP3-38231] - Western blot analysis of various lysates, using [KO Validated] Flotillin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-101220241252844.jpg "Western Blot: Flotillin-1 Antibody [NBP3-38231] - Western blot analysis of various lysates, using [KO Validated] Flotillin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunoprecipitation: Flotillin-1 Antibody [NBP3-38231] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug Flotillin-1 antibody. Western blot was performed from the immunoprecipitate using Flotillin-1 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-1012202411545540.jpg)
![Immunohistochemistry: Flotillin-1 Antibody [NBP3-38231] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using [KO Validated] Flotillin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-10122024121097.jpg)
![Immunohistochemistry: Flotillin-1 Antibody [NBP3-38231] - Immunohistochemistry analysis of paraffin-embedded Rat heart using [KO Validated] Flotillin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-101220241252843.jpg)
![Immunocytochemistry/ Immunofluorescence: Flotillin-1 Antibody [NBP3-38231] - Immunofluorescence analysis of NIH/3T3 cells using Flotillin-1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-10122024121062.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Flotillin-1 Antibody [NBP3-38231] - Western Blot analysis of lysates from wild type (WT) and Flotillin-1 knockout (KO) 293T cells, using [KO Validated] Flotillin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-311202516593618.jpg)
![Western Blot: Coronin-1a Antibody [NB110-58867] - Analysis of tissue lysates using Coronin-1a Antibody, dilution 1:5,000: [1] protein standard, [2] mouse brain, [3] rat brain, [4] cow cerebellum, [5] cow cortex, and [6] pig spinal cord. The strong single band about 55kDa corresponds to the Coronin-1a protein.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Western-Blot-NB110-58867-img0003.jpg "Western Blot: Coronin-1a Antibody [NB110-58867] - Analysis of tissue lysates using Coronin-1a Antibody, dilution 1:5,000: [1] protein standard, [2] mouse brain, [3] rat brain, [4] cow cerebellum, [5] cow cortex, and [6] pig spinal cord. The strong single band about 55kDa corresponds to the Coronin-1a protein.")
![Immunocytochemistry/Immunofluorescence: Coronin-1a Antibody [NB110-58867] - Analysis of cortical neuron-glial cell culture from E20 rat stained with Coronin-1a Antibody, dilution 1:1,000 (Red), and costained with mouse mAb to GFAP, dilution 1:1,000 (Green). Hoechst staining of nuclear DNA (Blue). The Coronin-1a Antibody labels protein expressed in the cytoplasm of microglia cells, while GFAP antibody stains intermediate filaments in astrocytic cells.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58867-img0004.jpg)
![Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - NEK9 monoclonal antibody (M01), clone 1F6. Analysis of NEK9 expression in NIH/3T3.](http://images.novusbio.com/fullsize/NEK9-Antibody-1F6-Western-Blot-H00091754-M01-img0014.jpg "Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - NEK9 monoclonal antibody (M01), clone 1F6. Analysis of NEK9 expression in NIH/3T3.")
![Immunocytochemistry/Immunofluorescence: NEK9 Antibody (1F6) [H00091754-M01] - Analysis of monoclonal antibody to NEK9 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NEK9-Antibody-1F6-Immunocytochemistry-Immunofluorescence-H00091754-M01-img0009.jpg)
![Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - NEK9 monoclonal antibody (M01), clone 1F6 Analysis of NEK9 expression in HeLa.](http://images.novusbio.com/fullsize/NEK9-Antibody-1F6-Western-Blot-H00091754-M01-img0011.jpg)
![Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - NEK9 monoclonal antibody (M01), clone 1F6. Analysis of NEK9 expression in PC-12.](http://images.novusbio.com/fullsize/NEK9-Antibody-1F6-Western-Blot-H00091754-M01-img0012.jpg)
![Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - NEK9 monoclonal antibody (M01), clone 1F6. Analysis of NEK9 expression in Raw 264.7.](http://images.novusbio.com/fullsize/NEK9-Antibody-1F6-Western-Blot-H00091754-M01-img0013.jpg)
![Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - Analysis of NEK9 expression in transfected 293T cell line by NEK9 monoclonal antibody (M01), clone 1F6.Lane 1: NEK9 transfected lysate(107.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NEK9-Antibody-1F6-Western-Blot-H00091754-M01-img0015.jpg)
![Sandwich ELISA: NEK9 Antibody (1F6) [H00091754-M01] - Detection limit for recombinant GST tagged NEK9 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NEK9-Antibody-1F6-Sandwich-ELISA-H00091754-M01-img0010.jpg)
![Western Blot: Use1/UBE2Z Antibody [NBP1-82785] - Analysis in human cell line RPMI-8226.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Western-Blot-NBP1-82785-img0007.jpg "Western Blot: Use1/UBE2Z Antibody [NBP1-82785] - Analysis in human cell line RPMI-8226.")
![Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP1-82785] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunohistochemistry-Paraffin-NBP1-82785-img0010.jpg)
![Western Blot: Use1/UBE2Z Antibody [NBP1-82785] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Western-Blot-NBP1-82785-img0005.jpg)
![Western Blot: Use1/UBE2Z Antibody [NBP1-82785] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Western-Blot-NBP1-82785-img0006.jpg)
![Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP1-82785] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunohistochemistry-Paraffin-NBP1-82785-img0002.jpg)
![Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP1-82785] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunohistochemistry-Paraffin-NBP1-82785-img0008.jpg)
![Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP1-82785] - Staining of human lymphoid node shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunohistochemistry-Paraffin-NBP1-82785-img0009.jpg)
![Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP1-82785] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunohistochemistry-Paraffin-NBP1-82785-img0011.jpg)
![Western Blot: PBK Antibody [NBP2-58043] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/PBK-Antibody-Western-Blot-NBP2-58043-img0002.jpg "Western Blot: PBK Antibody [NBP2-58043] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunohistochemistry-Paraffin: PBK Antibody [NBP2-58043] - Staining of human testis using Anti-PBK antibody NBP2-58043.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunohistochemistry-Paraffin-NBP2-58043-img0010.jpg)
![Immunohistochemistry-Paraffin: PBK Antibody [NBP2-58043] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunohistochemistry-Paraffin-NBP2-58043-img0003.jpg)
![Immunohistochemistry-Paraffin: PBK Antibody [NBP2-58043] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunohistochemistry-Paraffin-NBP2-58043-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PBK Antibody [NBP2-58043] - Staining in human testis and endometrium tissues using anti-PBK antibody. Corresponding PBK RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunohistochemistry-Paraffin-NBP2-58043-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PBK Antibody [NBP2-58043] - Staining of human colon, kidney, liver and testis using Anti-PBK antibody NBP2-58043 (A) shows similar protein distribution across tissues to independent antibody NBP1-84342 (B).](http://images.novusbio.com/fullsize/PBK-Antibody-Immunohistochemistry-Paraffin-NBP2-58043-img0006.jpg)
![Immunohistochemistry-Paraffin: PBK Antibody [NBP2-58043] - Staining of human kidney.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunohistochemistry-Paraffin-NBP2-58043-img0007.jpg)
![Immunohistochemistry-Paraffin: PBK Antibody [NBP2-58043] - Staining of human colon.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunohistochemistry-Paraffin-NBP2-58043-img0008.jpg)
![Immunohistochemistry-Paraffin: PBK Antibody [NBP2-58043] - Staining of human liver.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunohistochemistry-Paraffin-NBP2-58043-img0009.jpg)