| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11038 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75978SS | |||
| BSA Free | NBP2-75978 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70733 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48318 | |||
| Azide and BSA Free | NBP3-11498 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80036-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38323-25ul | |||
![Western Blot: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Western blot analysis of Jurkat cell lysate using Nucleolin antibody (NCL/7014R).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Western-Blot-NBP3-11037-img0003.jpg "Western Blot: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Western blot analysis of Jurkat cell lysate using Nucleolin antibody (NCL/7014R).")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded cow heart stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0010.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Human tonsil stained with Nucleolin Antibody. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0001.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Analysis of human cells administered in pig brain using Nucleolin antibody. Antibody did NOT stain porcine tissue allowing the presence of human cells to be determined. Primary antibody dilution 1:100. Image from verified customer review.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0002.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded human salivary gland stained with Nucleolin Antibody (NCL/7014R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0004.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Antibody (NCL/7014R).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0005.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Antibody (NCL/7014R).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0006.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded cat liver stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0007.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded horse lung stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0008.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded mouse stomach stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0009.jpg)
![Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Western-Blot-NBP2-80765-img0001.jpg "Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80765-img0002.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - HIF-1 alpha (clone 2443C) was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) at 1.0 ug/mL for 1 hour at room temperature followed by incubation with the](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80765-img0003.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Simple-Western-NBP2-80765-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Knockout-Validated-NBP2-80765-img0004.jpg)
![Western Blot: Fumarase Antibody (1F10) [NBP1-47754] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Fumarase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Western-Blot-NBP1-47754-img0007.jpg "Western Blot: Fumarase Antibody (1F10) [NBP1-47754] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Fumarase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: Fumarase Antibody (1F10) [NBP1-47754] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Fumarase.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Immunocytochemistry-Immunofluorescence-NBP1-47754-img0006.jpg)
![Immunohistochemistry: Fumarase Antibody (OTI1F10) [NBP1-47754] - Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human colon tissue using anti-Fumarase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-OTI1F10-Immunohistochemistry-NBP1-47754-img0008.jpg)
![Flow Cytometry: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Fumarase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Flow-Cytometry-NBP1-47754-img0001.jpg)
![Western Blot: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fumarase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Fumarase.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Western-Blot-NBP1-47754-img0002.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human endometrium tissue using anti-Fumarase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Immunohistochemistry-Paraffin-NBP1-47754-img0004.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human Kidney tissue using anti-Fumarase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Immunohistochemistry-Paraffin-NBP1-47754-img0005.jpg)
![Western Blot: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis using HK2 mouse mAb against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Western-Blot-NBP1-51643-img0010.jpg "Western Blot: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis using HK2 mouse mAb against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.")
![Immunohistochemistry: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Images of IHC analysis of B7-H3 and HK2 protein expression and hematoxylin and eosin (H&E) staining of CRC (n=126) tissue sections. One representative image is shown. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41419-019-1549-6), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Immunohistochemistry-NBP1-51643-img0012.jpg)
![Flow Cytometry: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis of K562 cells using HK2 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Flow-Cytometry-NBP1-51643-img0007.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis of esophagus cancer tissues (left) and human lung cancer (right) using HK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Immunohistochemistry-Paraffin-NBP1-51643-img0009.jpg)
![Western Blot: Hexokinase 2 Antibody (3D3) - BSA Free [NBP1-51643] - B7-H3 promoted the expression of HK2 in CRC cells.a, b The expression of glycolysis-related genes was detected by RT-qPCR in both B7-H3-overexpressing HCT116 (a) & RKO (b) cells. c, d The mRNA level of HK2 was detected by RT-qPCR in both HCT116 (c) & RKO (d) cells after transfection with siRNA NC, B7-H3 siRNA-1 or B7-H3 siRNA-2. e HK2 protein level & STAT3 activation (examined by the p-STAT3 expression level) were detected by western blot in both HCT116 & RKO cells after transfection with NC, B7-H3 siRNA-1 or B7-H3 siRNA-2. beta -actin served as a loading control. f HK2 protein level & STAT3 activation (examined by the p-STAT3 expression level) were detected by western blot in both B7-H3-overexpressing HCT116 & RKO cells. beta -actin served as a loading control. g Schematic representation of the proposed B7-H3/STAT3/HK2 axis. Values are expressed as means (SEMs). Five samples were analyzed per condition, & the experiments were performed in triplicate. *P < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30952834), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51643_mouse-monoclonal-hexokinase-2-antibody-3d3-310202416173538.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Hexokinase 2 Antibody (3D3) - BSA Free [NBP1-51643] - B7-H3 regulated glycolysis through HK2.a The protein level of HK2 in B7-H3-overexpressing HCT116 or RKO cells after transfection with siRNA negative control (NC) or HK2 siRNA transfection were analyzed by western blot. beta -actin served as a loading control. b, c Glucose consumption (b) & lactate production (c) were measured in B7-H3-overexpressing HCT116 or RKO cells transfected with NC or HK2 siRNA. d, e Glucose consumption (d) & lactate production (e) were measured in B7-H3-overexpressing HCT116 or RKO cells treated with PBS or 2-DG. Values are expressed as means (SEMs). Five samples were analyzed per condition, & the experiments were performed in triplicate. *P < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30952834), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51643_mouse-monoclonal-hexokinase-2-antibody-3d3-310202415175275.jpg)
![Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody [NBP2-16814] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hexokinase II stained by Hexokinase II antibody diluted at 1:2000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16814-img0017.jpg "Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody [NBP2-16814] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hexokinase II stained by Hexokinase II antibody diluted at 1:2000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse muscle. Hexokinase II stained by Hexokinase II antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0022.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - U87 xenograft. Hexokinase II antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Hexokinase-II-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0003.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse muscle. Hexokinase II antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0005.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat heart. Hexokinase II antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0007.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat stomach. Hexokinase II antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0008.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat colon. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0012.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat kidney. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0013.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse heart. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0014.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat muscle. Hexokinase II stained by Hexokinase II antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0015.jpg)
![Western Blot: Fumarase Antibody [NBP1-31336] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-31336-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Fumarase Antibody [NBP1-31336] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Fumarase Antibody [NBP1-31336] - Representative western blots showing protein expression of fumarase and key enzymes in OXPHOS. VDAC was used as a loading control (n = 3). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep22788) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-31336-img0018.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-31336] - Analysis of paraffin-embedded NCIN87 xenograft, using fumarate hydratase antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunohistochemistry-Paraffin-NBP1-31336-img0009.jpg)
![Western Blot: Fumarase Antibody [NBP1-31336] - A. 50 ug mouse liver lysate/extract 7.5% SDS-PAGE Fumarate hydratase antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-31336-img0010.jpg)
![Western Blot: Fumarase Antibody [NBP1-31336] - A.50 ug rat liver lysate/extract 10% SDS-PAGE Fumarate hydratase antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-31336-img0011.jpg)
![Immunocytochemistry/ Immunofluorescence: Fumarase Antibody [NBP1-31336] - Fumarase antibody detects Fumarase protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Fumarase stained by Fumarase antibody (NBP1-31336) diluted at 1:500.Blue: Fluoroshield with DAPI .Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-31336_rabbit-polyclonal-fumarase-antibody-910202420122069.jpg)
![Immunocytochemistry/Immunofluorescence: Fumarase Antibody [NBP1-89814] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89814-img0015.jpg "Immunocytochemistry/Immunofluorescence: Fumarase Antibody [NBP1-89814] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.")
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse hippocampus shows neuronal immunoreactivity in the CA3 layer.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342022.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse globus pallidus shows immunoreactivity in neurons.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-1752023133407.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse medulla shows positivity in the neurons of the facial nucleus.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342036.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse olfactory bulb shows neuronal positivity in the anterior olfactory nucleus.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-17520231334012.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342060.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-17520231334201.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-1752023133402.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342070.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Fumarase Antibody [NBP1-89814] - Analysis using Anti-FH antibody NBP1-89814 (A) shows similar pattern to independent antibody NBP1-89813 (B).](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-89814-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence analysis of HeLa cells stained with Nucleolin Mouse Monoclonal Antibody (SPM614) (green). Nuclei counterstained stained with DAPI (blue).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0006.jpg "Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence analysis of HeLa cells stained with Nucleolin Mouse Monoclonal Antibody (SPM614) (green). Nuclei counterstained stained with DAPI (blue).")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (SPM614) [NBP2-44611] - Human Tonsil stained with Nucleolin Monoclonal Antibody (SPM614).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunohistochemistry-Paraffin-NBP2-44611-img0001.jpg)
![Flow Cytometry: Nucleolin Antibody (SPM614) [NBP2-44611] - Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleolin Antibody (SPM614).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Flow-Cytometry-NBP2-44611-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence Analysis of HeLa cells stained with CF647R labeled Nucleolin Antibody (SPM614)(Green) Blue: DAPI.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (SPM614) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (SPM614) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0005.jpg)
![Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249374982.jpg "Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249383186.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog tissue. Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249355536.jpg)
![Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249403876.jpg)
![Immunocytochemistry/Immunofluorescence: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cytoplasm and nucleus and mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-212024937157.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat brain. Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249415929.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog brain. Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-212024941337.jpg)
![Western Blot: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1:2000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-OMNI379-Western-Blot-NBP2-80036-img0006.jpg "Western Blot: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1:2000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.")
![Immunocytochemistry/Immunofluorescence: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunofluorescent confocal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Nampt (Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-OMNI379-Immunocytochemistry-Immunofluorescence-NBP2-80036-img0004.jpg)
![Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunohistochemical staining of Nampt using anti-Nampt (OMNI379) in human kidney tissue (1:500 dilution). Immunoperoxidase staining of formalin-fixed and paraffin-embedded (FFPE) human kidney showing cytoplasmic staining (200x, brown color).](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-OMNI379-Immunohistochemistry-Paraffin-NBP2-80036-img0002.jpg)
![Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunohistochemical staining of Nampt using anti-Nampt (Visfatin/PBEF), mAb (OMNI379) in formalin-fixed and paraffin-embedded (FFPE) human colon tissue (15 ug/mL).](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-OMNI379-Immunohistochemistry-Paraffin-NBP2-80036-img0001.jpg)
![PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) at 1:2000 dilution. 1. Human Nampt (Visfatin/PBEF) (His-tagged). 2. Human Nampt (Visfatin/PBEF) (FLAG(R)-tagged). 3. Mouse Nampt (Visfatin/PBEF) (His-tagged). 4. Rat Nampt (Visfatin/PBEF) (His-tagged). 5. Human Nampt (Visfatin/PBEF) (His-tagged). 6. LPS-treated human peripheral blood leukocyte lysate.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-OMNI379-NBP2-80036-img0005.jpg)
![Immunoprecipitation: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted and visualized by western blot with anti-human Nampt (Visfatin/PBEF) pAb.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-OMNI379-Immunoprecipitation-NBP2-80036-img0007.jpg)
![Western Blot: Rpn2 Antibody [NBP2-38323] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/Rpn2-Antibody-Western-Blot-NBP2-38323-img0003.jpg "Western Blot: Rpn2 Antibody [NBP2-38323] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: Rpn2 Antibody [NBP2-38323] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & actin filaments.](http://images.novusbio.com/fullsize/Rpn2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38323-img0004.jpg)
![Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human liver.](http://images.novusbio.com/fullsize/Rpn2-Antibody-Immunohistochemistry-Paraffin-NBP2-38323-img0008.jpg)
![Immunohistochemistry: Rpn2 Antibody [NBP2-38323] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/Rpn2-Antibody-Immunohistochemistry-NBP2-38323-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human cerebral cortex, kidney, liver and testis using Anti-PSMD1 antibody NBP2-38323 (A) shows similar protein distribution across tissues to independent antibody NBP2-38324 (B).](http://images.novusbio.com/fullsize/Rpn2-Antibody-Immunohistochemistry-Paraffin-NBP2-38323-img0005.jpg)
![Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human testis.](http://images.novusbio.com/fullsize/Rpn2-Antibody-Immunohistochemistry-Paraffin-NBP2-38323-img0006.jpg)
![Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human kidney.](http://images.novusbio.com/fullsize/Rpn2-Antibody-Immunohistochemistry-Paraffin-NBP2-38323-img0007.jpg)
![Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Rpn2-Antibody-Immunohistochemistry-Paraffin-NBP2-38323-img0009.jpg)
![Western Blot: Aldolase A Antibody [NB600-915] - Analysis using Biotin conjugate of NB600-915. 300 ul aliquots of whole anti-aldolase antiserum used to precipitate varying amounts of purified aldolase & precipitates with controls; compared by SDS-PAGE & Western blot. 1. Purified aldolase 2. 300 ul antiserum with no antigen (negative control) 3. 300 ul antiserum with 100 ul aldolase (2.5 mg/ml) 4. 300 ul antiserum with 200 ul aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum & an equal volume of aldolase antigen in PBS incubated 24 hrs at 4C, centrifuged for 6 mins at 13K RPM, washed once with PBS, centrifuged & dissolved in 60 ul 0.1 N NaOH. 90 ul of PBS was added, sample was divided in 2 portions, & an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer added. The reduced samples were boiled for five mins, & all samples were run at 140 V for 45 mins on a 4-20% tris/glycine gradient gel. Gel was stained, destained & imaged.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Western-Blot-NB600-915-img0010.jpg "Western Blot: Aldolase A Antibody [NB600-915] - Analysis using Biotin conjugate of NB600-915. 300 ul aliquots of whole anti-aldolase antiserum used to precipitate varying amounts of purified aldolase & precipitates with controls; compared by SDS-PAGE & Western blot. 1. Purified aldolase 2. 300 ul antiserum with no antigen (negative control) 3. 300 ul antiserum with 100 ul aldolase (2.5 mg/ml) 4. 300 ul antiserum with 200 ul aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum & an equal volume of aldolase antigen in PBS incubated 24 hrs at 4C, centrifuged for 6 mins at 13K RPM, washed once with PBS, centrifuged & dissolved in 60 ul 0.1 N NaOH. 90 ul of PBS was added, sample was divided in 2 portions, & an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer added. The reduced samples were boiled for five mins, & all samples were run at 140 V for 45 mins on a 4-20% tris/glycine gradient gel. Gel was stained, destained & imaged.")
![Western Blot: Aldolase A Antibody [NB600-915] - Figure. Immunoblotting. IgG purified antibody to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4 degrees C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Western-Blot-NB600-915-img0003.jpg)
![Western Blot: Aldolase A Antibody [NB600-915] - IgG purified antibody to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4 C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE).](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Western-Blot-NB600-915-img0009.jpg)
![Immunoprecipitation: Aldolase A Antibody [NB600-915] - Analysis using the Biotin conjugate of NB600-915. Detection of 300 ul of anti aldolase antiserum and an equal volume of varied amounts (diluted from a stock solution of ~2.5 ug/ml) of purified aldolase in PBS. Precipitation of aldolase was confirmed.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Immunoprecipitation-NB600-915-img0005.jpg)
![Western Blot: Aldolase A Antibody [NB600-915] - Twenty one more markedly oxidized proteins identified in 6‐week‐old PLP‐tg66/66 mice in the spinal cord. All non‐mitochondrial oxidized proteins tested by Western blot in the spinal cord are normally expressed. Relative protein level expressed as a percentage of control and in reference to gamma ‐tubulin as a loading marker (n = 6/genotype). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29027761/) licensed under a CC-BY license](http://images.novusbio.com/fullsize/nb600-915_goat-polyclonal-aldolase-a-antibody-2552023153959.jpg)