| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75477SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-61646SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27177SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86805-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90365-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27403 | |||
| BSA Free | NB100-56546SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88146-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82648-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71688 | |||
![Western Blot: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP1-75477-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP1-75477] - Analysis of Jurkat cell lysate (30ug) using anti-Hsp27 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP1-75477-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP1-75477] - Hsp27 Antibody [NBP1-75477] - HSP27 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-75477-img0006.jpg)
![Immunohistochemistry: Hsp27 Antibody [NBP1-75477] - Staining of HSP27 in mouse heart using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunohistochemistry-NBP1-75477-img0007.jpg)
![Western Blot: Hsp27 Antibody [NBP1-75477] - Analysis of HSP27 in human skeletal muscle lysate.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP1-75477-img0008.jpg)
![Western Blot: EGFR Antibody [NBP1-84814] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/EGFR-Antibody-Western-Blot-NBP1-84814-img0009.jpg "Western Blot: EGFR Antibody [NBP1-84814] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: EGFR Antibody [NBP1-84814] - Staining of human cell line A-431 shows positivity in plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84814-img0006.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84814] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84814-img0017.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84814] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84814-img0015.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84814] - Staining of human cerebellum shows no positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84814-img0016.jpg)
![Western Blot: Nucleophosmin Antibody [NB110-61646] - Detection of mutant Nucleophosmin in OCI-AML3 lysates.](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-Western-Blot-NB110-61646-img0012.jpg "Western Blot: Nucleophosmin Antibody [NB110-61646] - Detection of mutant Nucleophosmin in OCI-AML3 lysates.")
![Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in HL-60 cells (negative control).](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61646-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in OCI-AML3 cells.](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61646-img0010.jpg)
![Western Blot: AIF Antibody [NBP2-27177] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and AIF knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human AIF Polyclonal Antibody (Catalog # NBP2-27177) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 68 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/AIF-Antibody-Knockout-Validated-NBP2-27177-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: AIF Antibody [NBP2-27177] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and AIF knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human AIF Polyclonal Antibody (Catalog # NBP2-27177) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 68 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: AIF Antibody [NBP2-27177] - Analysis of AIF in human colon using this antibody. Lane 1, normal colon. Lanes 2-7, colon carcinoma tissue lysatesfrom 6 different patients. mAIF, thought to represent mitochondrial AIF.](http://images.novusbio.com/fullsize/AIF-Antibody-Western-Blot-NBP2-27177-img0001.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody [NBP2-27177] - Staining of AIF in formalin-fixed, paraffin-embedded normal human colon using this antibody at 1:2000. Hematoxylin-eosin counterstain. In this example, AIF expression is predominant in the upper part of the crypt.](http://images.novusbio.com/fullsize/AIF-Antibody-Immunohistochemistry-Paraffin-NBP2-27177-img0002.jpg)
![Simple Western: AIF Antibody [NBP2-27177] - Simple Western lane view shows a specific band for AIF in 0.2 mg/ml of Jurkat and MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/fullsize/AIF-Antibody-Simple-Western-NBP2-27177-img0003.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0026.jpg "Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human ovarian carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0023.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug K562 whole cell lysate/extract B. 30 ug THP-1 whole cell lysate/extract 10% SDS-PAGE Bmi1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0019.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug C8D30 whole cell lysate/extract. B. 30 ug BCL-1 whole cell lysate/extract. C. 30 ug Raw264.7 whole cell lysate/extract. 10 % SDS-PAGE. Dilution: 1:1000.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0020.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug THP-1 whole cell lysate/extract. B. 30 ug HL-60 whole cell lysate/extract.10 % SDS-PAGE. Dilution: 1:1000](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0021.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human breast carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0022.jpg)
![Immunoprecipitation: BMI-1 Antibody [NBP1-33748] - Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of BMI-1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of BMI-1 protein by 2.5 ug of BMI-1antibody 10% SDS-PAGE The immunoprecipitated BMI-1protein was detected by BMI-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunoprecipitation-NBP1-33748-img0018.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-9102024209145.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded dog lung.BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420115062.jpg)
![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at nucleus by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202419593519.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420131285.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-91020242021927.jpg)
![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at nucleus by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420134048.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-91020242063358.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BMI-1 Antibody [NBP1-33748] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-1610202419414590.jpg)
![BMI1 antibody [N1C3-2] detects BMI1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: BMI1 protein stained by BMI1 antibody (NBP1-33748) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-immunocytochemistry-immunofluorescence-261120251844101.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human cerebral cortex, gallbladder, kidney and lymph node using Anti-EML4 antibody NBP1-86805 (A) shows similar protein distribution across tissues to independent antibody NBP2-38319 (B).](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0007.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human lymph node.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0010.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0008.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human kidney.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0009.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human gallbladder.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0011.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-86805_rabbit-polyclonal-eml4-antibody-304202513101431.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Analysis in human placenta and skeletal muscle tissues. Corresponding TOP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0015.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Staining of human placenta shows very strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0018.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0019.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0016.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Staining of human lymphoid tissues shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0017.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Western-Blot-NBP2-27403-img0003.jpg "Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Chk2 (73C175.1.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the standard format of this product.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-27403-img0006.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Immunohistochemistry-Paraffin-NBP2-27403-img0002.jpg)
![Flow Cytometry: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - An intracellular stain was performed on Hek293 cells with Chk2 [73C175.1.1] Antibody NB100-56546 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550. Image using the standard format of this product.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Flow-Cytometry-NBP2-27403-img0005.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Western-Blot-NBP2-27403-img0001.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml. Image using the Azide Free form of this antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Western-Blot-NB100-56546-img0003.jpg "Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml. Image using the Azide Free form of this antibody.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (73C175.1.1) [NB100-56546] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Chk2 (73C175.1.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Immunocytochemistry-Immunofluorescence-NB100-56546-img0004.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (73C175.1.1) [NB100-56546] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Immunohistochemistry-Paraffin-NB100-56546-img0002.jpg)
![Flow Cytometry: Chk2 Antibody (73C175.1.1) [NB100-56546] - An intracellular stain was performed on Hek293 cells with Chk2 [73C175.1.1] Antibody NB100-56546 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Flow-Cytometry-NB100-56546-img0006.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Western-Blot-NB100-56546-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining of human cerebral cortex, skeletal muscle, testis and tonsil using Anti-CCT2 antibody NBP1-88146 (A) shows similar protein distribution across tissues to independent antibody NBP1-88147 (B).](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-88146-img0014.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining in human testis and skeletal muscle tissues using anti-CCT2 antibody. Corresponding CCT2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-88146-img0012.jpg)
![Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-88146-img0011.jpg)
![Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-88146-img0010.jpg)
![Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining of human tonsil.](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-88146-img0017.jpg)
![Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-88146-img0016.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: TCP1-beta Antibody [NBP1-88146] - Analysis using Anti-CCT2 antibody NBP1-88146 (A) shows similar pattern to independent antibody NBP1-88147 (B).](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Western-Blot-NBP1-88146-img0015.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/nbp1-88146_rabbit-polyclonal-tcp1-beta-antibody-6520251624419.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/nbp1-88146_rabbit-polyclonal-tcp1-beta-antibody-65202516252810.jpg)
![Western Blot: DHPS Antibody [NBP1-82648] - Analysis in control (vector only transfected HEK293T lysate) and DHPS over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/DHPS-Antibody-Western-Blot-NBP1-82648-img0008.jpg "Western Blot: DHPS Antibody [NBP1-82648] - Analysis in control (vector only transfected HEK293T lysate) and DHPS over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: DHPS Antibody [NBP1-82648] - Staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DHPS-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82648-img0007.jpg)
![Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82648] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/DHPS-Antibody-Immunohistochemistry-Paraffin-NBP1-82648-img0012.jpg)
![Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82648] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DHPS-Antibody-Immunohistochemistry-Paraffin-NBP1-82648-img0009.jpg)
![Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82648] - Staining of human fallopian tube shows moderate cytoplasmic/membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/DHPS-Antibody-Immunohistochemistry-Paraffin-NBP1-82648-img0010.jpg)
![Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82648] - Staining of human pancreas shows weak cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/DHPS-Antibody-Immunohistochemistry-Paraffin-NBP1-82648-img0011.jpg)
![Western Blot: Chk2 Antibody (OTI5C4) [NBP1-47695] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Chk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Chk2.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Western-Blot-NBP1-47695-img0020.jpg "Western Blot: Chk2 Antibody (OTI5C4) [NBP1-47695] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Chk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Chk2.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (OTI5C4) [NBP1-47695] - Chk2 Antibody (5C4) [NBP1-47695] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Chk2.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunocytochemistry-Immunofluorescence-NBP1-47695-img0013.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human pancreas tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0019.jpg)
![Flow Cytometry: Chk2 Antibody (OTI5C4) [NBP1-47695] - Analysis of Jurkat cells, using anti-Chk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Flow-Cytometry-NBP1-47695-img0012.jpg)
![Western Blot: Chk2 Antibody (OTI5C4) [NBP1-47695] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Chk2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Western-Blot-NBP1-47695-img0011.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human breast tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0014.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human colon tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0015.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human Kidney tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0016.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human liver tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0017.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human lung tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0018.jpg)