| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85804-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88648-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87926-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43948 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74052 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-154SS | |||
| Azide and BSA Free | NBP2-80871 | |||
![Immunocytochemistry/Immunofluorescence: Lck Antibody [NBP1-85804] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Lck-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85804-img0005.jpg "Immunocytochemistry/Immunofluorescence: Lck Antibody [NBP1-85804] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.")
![Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/nbp1-85804_rabbit-polyclonal-lck-antibody-2442025930833.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining in human cerebral cortex and prostate tissues using NBP1-88648 antibody. Corresponding FABP7 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-88648-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining in human cerebral cortex and prostate tissues using NBP1-88648 antibody. Corresponding FABP7 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: FABP7/B-FABP Antibody [NBP1-88648] - Analysis in human cell line U-251 MG.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Western-Blot-NBP1-88648-img0013.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining of human cerebellum shows strong cytoplasmic positivity in Bergmann glia.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-88648-img0014.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-88648-img0015.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining of human glioma shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-88648-img0016.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-88648-img0017.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-88648-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Flightless I Antibody [NBP1-87926] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & microtubule organizing center.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87926-img0010.jpg "Immunocytochemistry/Immunofluorescence: Flightless I Antibody [NBP1-87926] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & microtubule organizing center.")
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87926] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87926-img0014.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87926] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87926-img0011.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87926] - Staining of human gastrointestinal shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87926-img0012.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87926] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87926-img0013.jpg)
![Western Blot: Flightless I Antibody [NBP1-87926] - Analysis in human cell line A-549.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Western-Blot-NBP1-87926-img0006.jpg)
![Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/PI-3-Kinase-p110-beta-PIK3CB-Antibody-CL9518-Western-Blot-NBP2-88937-img0008.jpg "Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Analysis in human cell line HEL.")
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Staining of A-431 cells using the Anti-PIK3CB monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/PI-3-Kinase-p110-beta-PIK3CB-Antibody-CL9518-Immunocytochemistry-Immunofluorescence-NBP2-88937-img0001.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Staining of human thyroid gland shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PI-3-Kinase-p110-beta-PIK3CB-Antibody-CL9518-Immunohistochemistry-Paraffin-NBP2-88937-img0007.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Staining of human cervix shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/PI-3-Kinase-p110-beta-PIK3CB-Antibody-CL9518-Immunohistochemistry-Paraffin-NBP2-88937-img0002.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Staining of human colonshows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PI-3-Kinase-p110-beta-PIK3CB-Antibody-CL9518-Immunohistochemistry-Paraffin-NBP2-88937-img0003.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/PI-3-Kinase-p110-beta-PIK3CB-Antibody-CL9518-Immunohistochemistry-Paraffin-NBP2-88937-img0004.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Staining of human stomach cancer shows moderate to strong nuclear positivity.](http://images.novusbio.com/fullsize/PI-3-Kinase-p110-beta-PIK3CB-Antibody-CL9518-Immunohistochemistry-Paraffin-NBP2-88937-img0005.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PI-3-Kinase-p110-beta-PIK3CB-Antibody-CL9518-Immunohistochemistry-Paraffin-NBP2-88937-img0006.jpg)
![Western Blot: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Secernin 1.](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Western-Blot-NBP2-45727-img0010.jpg "Western Blot: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Secernin 1.")
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0009.jpg)
![Western Blot: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Western-Blot-NBP2-45727-img0011.jpg)
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0001.jpg)
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0002.jpg)
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0003.jpg)
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0004.jpg)
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0005.jpg)
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0006.jpg)
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0007.jpg)
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0008.jpg)
![Western Blot: MKP-1/DUSP1 Antibody [NB100-834] - Staining of HeLa cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-Western-Blot-NB100-834-img0004.jpg "Western Blot: MKP-1/DUSP1 Antibody [NB100-834] - Staining of HeLa cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody [NB100-834] - Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-Immunohistochemistry-Paraffin-NB100-834-img0006.jpg)
![Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody [NB100-834] - Staining of paraffin embedded Human Prostate. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-Immunohistochemistry-Paraffin-NB100-834-img0005.jpg)
![Western Blot: IFT88 Antibody [NB100-2475] - Staining of A549 (A) cell lysate and Mouse Testes (B) lysate (35 ug protein in RIPA buffer). Antibody at 3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/IFT88-Antibody-Western-Blot-NB100-2475-img0008.jpg "Western Blot: IFT88 Antibody [NB100-2475] - Staining of A549 (A) cell lysate and Mouse Testes (B) lysate (35 ug protein in RIPA buffer). Antibody at 3 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: IFT88 Antibody [NB100-2475] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/IFT88-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2475-img0009.jpg)
![Flow Cytometry: IFT88 Antibody [NB100-2475] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/IFT88-Antibody-Flow-Cytometry-NB100-2475-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: PDGF-B Antibody [NBP1-52533] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/PDGF-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-52533-img0005.jpg "Immunocytochemistry/Immunofluorescence: PDGF-B Antibody [NBP1-52533] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry-Paraffin: PDGF-B Antibody [NBP1-52533] - Analysis of anti-PDGFB antibody with human pancreas at concentration 3.75 ug/ml.](http://images.novusbio.com/fullsize/PDGF-B-Antibody-Immunohistochemistry-Paraffin-NBP1-52533-img0004.jpg)
![Flow Cytometry: PDGF-B Antibody [NBP1-52533] - Flow cytometric analysis of fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PDGF-B-Antibody-Flow-Cytometry-NBP1-52533-img0006.jpg)
![Immunohistochemistry-Paraffin: PDGF-B Antibody [NBP1-52533] - Analysis of anti-PDGFB antibody with human placenta at concentration 3.75 ug/ml.](http://images.novusbio.com/fullsize/PDGF-B-Antibody-Immunohistochemistry-Paraffin-NBP1-52533-img0003.jpg)
![Immunohistochemistry-Frozen: Uromodulin Antibody (10.32) [NBP1-50321] - Human kidney tissue section stained with Uromodulin antibody. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Uromodulin-Antibody-10-32-Immunohistochemistry-Frozen-NBP1-50321-img0003.jpg "Immunohistochemistry-Frozen: Uromodulin Antibody (10.32) [NBP1-50321] - Human kidney tissue section stained with Uromodulin antibody. IHC-Fr image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: Uromodulin Antibody (10.32) [NBP1-50321] - Analysis of anti-UMOD / Uromodulin antibody with human kidney at concentration 20 ug/mL.](http://images.novusbio.com/fullsize/Uromodulin-Antibody-10-32-Immunohistochemistry-Paraffin-NBP1-50321-img0001.jpg)
![Immunohistochemistry-Paraffin: Uromodulin Antibody (10.32) [NBP1-50321] - Human kidney tissue, staining in kidney tubules. Heat mediated antigen retrieval was performed by heating in citrate buffer (pH 6) at 95C for 20 minutes. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Uromodulin-Antibody-10-32-Immunohistochemistry-Paraffin-NBP1-50321-img0002.jpg)
![Western Blot: Calpain 1 Antibody [NBP2-15671] - Calpain 1 antibody detects Calpain 1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 1 antibody diluted at a dilution of 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Calpain-1-Antibody-Western-Blot-NBP2-15671-img0006.jpg "Western Blot: Calpain 1 Antibody [NBP2-15671] - Calpain 1 antibody detects Calpain 1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 1 antibody diluted at a dilution of 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Calpain 1 Antibody [NBP2-15671] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Calpain-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15671-img0004.jpg)
![Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15671] - Mouse lung. Calpain 1 antibody [N3C2], Internal diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Calpain-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15671-img0007.jpg)
![Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15671] - Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Calpain-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15671-img0003.jpg)
![Immunoprecipitation: Calpain 1 Antibody [NBP2-15671] - Immunoprecipitation of Calpain 1 protein from A431 whole cell extracts using 5 ug of Calpain 1 antibody. Western blot analysis was performed using Calpain 1 antibody.](http://images.novusbio.com/fullsize/Calpain-1-Antibody-Immunoprecipitation-NBP2-15671-img0005.jpg)
![Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27b) [NB300-154] - Staining of adult human dorsal root ganglia.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27b-Immunohistochemistry-Paraffin-NB300-154-img0002.jpg "Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27b) [NB300-154] - Staining of adult human dorsal root ganglia.")
![Western Blot: HSP27 [p Ser78] Antibody [NBP2-24458] - Analysis of HSP27 (Ser78) in recombinant fusion protein containing 1) a phosphorylated serine at position 78 and 2) an unphosphorylated serine at position 78, using HSP27 phospho antibody at 0.01 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Hsp27-[p-Ser78]-Antibody-Western-Blot-NBP2-24458-img0001.jpg "Western Blot: HSP27 [p Ser78] Antibody [NBP2-24458] - Analysis of HSP27 (Ser78) in recombinant fusion protein containing 1) a phosphorylated serine at position 78 and 2) an unphosphorylated serine at position 78, using HSP27 phospho antibody at 0.01 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")