| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89917-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71629 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40823SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54546-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56341SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47769-0.1mg | |||
| IHC-Prediluted | NBP2-48399 | |||
![Immunocytochemistry/Immunofluorescence: p57 Kip2 Antibody [NBP1-89917] - Staining of human cell line U-2 OS shows localization to nucleoplasm, nuclear bodies & cytosol.](http://images.novusbio.com/fullsize/p57-Kip2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89917-img0011.jpg "Immunocytochemistry/Immunofluorescence: p57 Kip2 Antibody [NBP1-89917] - Staining of human cell line U-2 OS shows localization to nucleoplasm, nuclear bodies & cytosol.")
![Western Blot: ALDH1L1 Antibody (3D2) [NBP2-03143] Analysis of extracts (35ug) from 9 different cell lines by using anti-ALDH1L1 monoclonal antibody.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-3D2-Western-Blot-NBP2-03143-img0008.jpg "Western Blot: ALDH1L1 Antibody (3D2) [NBP2-03143] Analysis of extracts (35ug) from 9 different cell lines by using anti-ALDH1L1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: ALDH1L1 Antibody (3D2) [NBP2-03143] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH1L1.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-3D2-Immunocytochemistry-Immunofluorescence-NBP2-03143-img0007.jpg)
![Immunohistochemistry-Paraffin: ALDH1L1 Antibody (3D2) [NBP2-03143] - Staining of paraffin-embedded Human prostate tissue using anti-ALDH1L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-3D2-Immunohistochemistry-Paraffin-NBP2-03143-img0006.jpg)
![Western Blot: ALDH1L1 Antibody (3D2) [NBP2-03143] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH1L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH1L1.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-3D2-Western-Blot-NBP2-03143-img0001.jpg)
![Immunohistochemistry-Paraffin: ALDH1L1 Antibody (3D2) [NBP2-03143] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ALDH1L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-3D2-Immunohistochemistry-Paraffin-NBP2-03143-img0002.jpg)
![Immunohistochemistry-Paraffin: ALDH1L1 Antibody (3D2) [NBP2-03143] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ALDH1L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-3D2-Immunohistochemistry-Paraffin-NBP2-03143-img0003.jpg)
![Immunohistochemistry-Paraffin: ALDH1L1 Antibody (3D2) [NBP2-03143] - Staining of paraffin-embedded Human Kidney tissue using anti-ALDH1L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-3D2-Immunohistochemistry-Paraffin-NBP2-03143-img0004.jpg)
![Immunohistochemistry-Paraffin: ALDH1L1 Antibody (3D2) [NBP2-03143] - Staining of paraffin-embedded Human liver tissue using anti-ALDH1L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-3D2-Immunohistochemistry-Paraffin-NBP2-03143-img0005.jpg)
![Western Blot: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of 25 ug of various brain, SHSY5Y, and HeLa cell lysates and 10ul of PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Western-Blot-NBP2-37816-img0014.jpg "Western Blot: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of 25 ug of various brain, SHSY5Y, and HeLa cell lysates and 10ul of PageRuler Prestained Protein Ladder.")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of beta-3 Tubulin in SHSY5Y cells (human neuroblast) either left untreated (left panel) or treated with 10uM retinoic acid (right panel) for 72 hours. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a beta-3 Tubulin monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Immunocytochemistry-Immunofluorescence-NBP2-37816-img0011.jpg)
![Immunohistochemistry: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Normal biopsies of deparaffinized human brain tissues.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Immunohistochemistry-NBP2-37816-img0012.jpg)
![Flow Cytometry: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of beta-3 Tubulin (blue histogram) on human SHSY5Y neuroblast cells. Cells were harvested, fixed with 4% formaldehyde, washed with PBS, and incubated with a beta-3 Tubulin monoclonal antibody at a 1:50 dilution or PBS alone (red histogram) for 1 hour at room temperature. For flow cytometry analysis, a 30-minute incubation with DyLight 488 goat anti-mouse IgG secondary antibody was performed and 10,000 cells were acquired for each sample.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Flow-Cytometry-NBP2-37816-img0010.jpg)
![Immunoprecipitation: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of beta-3 Tubulin was performed using SHSY5Y whole cell lysate. Antigen-antibody complexes were formed by incubating 300ug of lysate with 5ug of a beta-3 Tubulin monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. SHSY5Y cell lysate (30ug) was loaded as a positive control (left lane). The sample was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a beta-3 Tubulin monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Immunoprecipitation-NBP2-37816-img0013.jpg)
![Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Western-Blot-NBP2-67378-img0008.jpg "Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Staining ERK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67378-img0004.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67378-img0007.jpg)
![Flow Cytometry: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of Jurkat cells with ERK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Flow-Cytometry-NBP2-67378-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Staining ERK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67378-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Staining ERK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67378-img0003.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of paraffin-embedded human breast tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67378-img0005.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of paraffin-embedded human colon cancer tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67378-img0006.jpg)
![Western Blot: BMI-1 Antibody [NBP2-24693] - Analysis of BMI1 in skeletal muscle lysate. Lane 1 shows pre-immune sera. Lanes 2, 3 and 4 show this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP2-24693-img0006.jpg "Western Blot: BMI-1 Antibody [NBP2-24693] - Analysis of BMI1 in skeletal muscle lysate. Lane 1 shows pre-immune sera. Lanes 2, 3 and 4 show this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody [NBP2-24693] - Immunofluorescence of BMI1 in human oral squamous cell carcinoma (UPCI:SCC029B) cells. These cells are part of a large collection of HNSCC (head and neck SCC) lines established by the Gollin lab (White et al., 2007). The BMI1 antibody was used at 5 ug/ml. Staining is primarily nuclear, some cytoplasmic staining is also observed. Data courtesy of Hatem Kaseb, MD and Susanne M. Gollin, PhD.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24693-img0004.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP2-24693] - Analysis of BMI1 in formalin-fixed, paraffin-embedded human cervix tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP2-24693-img0005.jpg)
![Western Blot: Bmi1 Antibody [NB110-40823] - Detection of Bmi1 protein in skeletal muscle](http://images.novusbio.com/fullsize/Bmi1-Antibody-Western-Blot-NB110-40823-img0002.jpg "Western Blot: Bmi1 Antibody [NB110-40823] - Detection of Bmi1 protein in skeletal muscle")
![Immunocytochemistry/Immunofluorescence: Bmi1 Antibody [NB110-40823] - BMI1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Bmi1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40823-img0004.jpg)
![Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw 264.7 whole cell lysate/extract. G. 30 ug C2Cl2 whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000](http://images.novusbio.com/fullsize/SET-Antibody-Western-Blot-NBP1-33713-img0008.jpg "Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw 264.7 whole cell lysate/extract. G. 30 ug C2Cl2 whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000")
![Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by Western blot analysis. A. 30 ug A431 whole cell lysate/extract whole cell lysate/extract. B. 30 ug H1299 whole cell lysate/extract. C. 30 ug HeLa whole cell lysate/extract. D. 30 ug HepG2 whole cell lysate/extract. E. 30 ug Molt-4 whole cell lysate/extract. F. 30 ug Raji whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000 SET antibody detects SET protein by Western blot analysis.Observed SET protein is larger than the predicted M.W., possibly due to post-translational](http://images.novusbio.com/fullsize/SET-Antibody-Western-Blot-NBP1-33713-img0004.jpg)
![Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/SET-Antibody-Western-Blot-NBP1-33713-img0005.jpg)
![Immunoprecipitation: SET Antibody [NBP1-33713] - SET antibody immunoprecipitates SET protein in IP experiments. IP samples: HeLa whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of SET protein by 4 ug SET antibody. 10 % SDS-PAGE. The immunoprecipitated SET protein was detected by SET antibody diluted at 1:500.](http://images.novusbio.com/fullsize/SET-Antibody-Immunoprecipitation-NBP1-33713-img0006.jpg)
![Western Blot: SET Antibody [NBP1-33713] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody (NBP1-33713) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33713_rabbit-polyclonal-set-antibody-910202420115093.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SET Antibody [NBP1-33713] - Non-transfected (–) and transfected (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp1-33713_rabbit-polyclonal-set-antibody-1610202419414526.jpg)
![SET antibody detects SET protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SET protein stained by SET antibody (NBP1-33713) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-33713_rabbit-polyclonal-set-antibody-immunocytochemistry-immunofluorescence-2611202518431924.jpg)
![Western Blot: TOP2A Antibody (TOP2A/1361) [NBP2-53281] - Western Blot Analysis of human HeLa cell lysate using TOP2A Antibody (TOP2A/1361).](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1361-Western-Blot-NBP2-53281-img0006.jpg "Western Blot: TOP2A Antibody (TOP2A/1361) [NBP2-53281] - Western Blot Analysis of human HeLa cell lysate using TOP2A Antibody (TOP2A/1361).")
![Immunocytochemistry/Immunofluorescence: TOP2A Antibody (TOP2A/1361) [NBP2-53281] - Confocal Immunofluorescence image of HeLa cells using TOP2A Antibody (TOP2A/1361) Green (CF488) and Phalloidin (Red) is used to label the nuclei.](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1361-Immunocytochemistry-Immunofluorescence-NBP2-53281-img0004.jpg)
![Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1361) [NBP2-53281] - Formalin-fixed, paraffin-embedded Human Tonsil stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1361).](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1361-Immunohistochemistry-Paraffin-NBP2-53281-img0002.jpg)
![Western Blot: TOP2A Antibody (TOP2A/1361) [NBP2-53281] - Western Blot of HepG2, HeLa and 3T3 Cell Lysate using Topo II alpha, Monoclonal Antibody (TOP2A/1361).](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1361-Western-Blot-NBP2-53281-img0003.jpg)
![Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1361) [NBP2-53281] - Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1361).](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1361-Immunohistochemistry-Paraffin-NBP2-53281-img0001.jpg)
![Protein Array: TOP2A Antibody (TOP2A/1361) [NBP2-53281] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TOP2A Antibody (TOP2A/1361). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1361-Protein-Array-NBP2-53281-img0005.jpg)
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NB100-56341] - Analysis of HDAC-4 in Jurkat cell lysate with this antibody.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-Western-Blot-NB100-56341-img0003.jpg "Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NB100-56341] - Analysis of HDAC-4 in Jurkat cell lysate with this antibody.")
![Western Blot: SASH1 Antibody [NBP1-26650] - Whole cell lysate (50 ug) from HeLa, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SASH1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SASH1-Antibody-Western-Blot-NBP1-26650-img0004.jpg "Western Blot: SASH1 Antibody [NBP1-26650] - Whole cell lysate (50 ug) from HeLa, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SASH1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Western Blot: SASH1 Antibody [NBP1-26650] - Whole cell lysate from HeLa cells. SASH1 was also immunoprecipitated by rabbit anti-SASH1 antibody NBP1-26651.](http://images.novusbio.com/fullsize/SASH1-Antibody-Western-Blot-NBP1-26650-img0003.jpg)
![Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: LNCaP prostate cancer cells. Load: 50 ug per lane.Primary antibody: HDAC1 antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: 55kDa for HDAC1.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-78101-img0012.jpg "Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: LNCaP prostate cancer cells. Load: 50 ug per lane.Primary antibody: HDAC1 antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: 55kDa for HDAC1.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-78101] - Immunofluorescence Microscopy of HDAC1 antibody. Tissue: A431 cells. Fixation: methanol. Antigen retrieval: blocked with normal goat serum.Primary antibody: HDAC1 antibody at 4 ug/mL for 1 h at RT.Secondary antibody: rabbit secondary antibody at 0.2 ug/mL for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 as green fluorescent signal. A-tubulin monoclonal antibody detected with ATTO 425 (colored RED). 2-color STED image, Leica Microsystems.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78101-img0010.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-78101] - Immunohistochemistry of HDAC1 antibody. Tissue: human lung tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: HDAC1 antibody at 10 ug/mL for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 as brown color indicates presence of protein, blue color shows cell nuclei.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-NBP1-78101-img0014.jpg)
![Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: 293 whole cell lysate. Load: 35 ug per lane.Primary antibody: HDAC1 antibody at 1:3,500 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: ~65 kDa corresponding to human HDAC1. Other band(s): none.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-78101-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-78101] - Rabbit IgG 611-151-122 (shown in red) for to detect HDAC-1 by immunofluorescence. Anti Keratin monoclonal antibody was used with Dylight 488 goat anti mouse (shown in green) to detect Keratin](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78101-img0004.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-78101] - Immunohistochemistry of HDAC1 antibody. Tissue: human prostate cancer tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: HDAC1 antibody at 1:500 for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 precipitated purple with blue counterstain.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-NBP1-78101-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (KIP1/769) [NBP2-44495] - Analysis of PFA-fixed MCF-7 cells labeling p27 with p27/Kip1 Antibody (KIP1/769) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-KIP1-769-Immunocytochemistry-Immunofluorescence-NBP2-44495-img0007.jpg "Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (KIP1/769) [NBP2-44495] - Analysis of PFA-fixed MCF-7 cells labeling p27 with p27/Kip1 Antibody (KIP1/769) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.")
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) [NBP2-44495] - Rat Colon stained with p27 Monoclonal Antibody (KIP1/769)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-KIP1-769-Immunohistochemistry-Paraffin-NBP2-44495-img0005.jpg)
![Flow Cytometry: p27/Kip1 Antibody (KIP1/769) [NBP2-44495] - Human p27 on Jurkat Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled p27 Monoclonal Antibody (KIP1/769).](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-KIP1-769-Flow-Cytometry-NBP2-44495-img0001.jpg)
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) [NBP2-44495] - Human Cervical cancer stained with p27 Monoclonal Antibody (KIP1/769)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-KIP1-769-Immunohistochemistry-Paraffin-NBP2-44495-img0002.jpg)
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) [NBP2-44495] - Human Colon Carcinoma stained with p27 Monoclonal Antibody (KIP1/769)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-KIP1-769-Immunohistochemistry-Paraffin-NBP2-44495-img0003.jpg)
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) [NBP2-44495] - Human Prostate cancer stained with p27 Monoclonal Antibody (KIP1/769)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-KIP1-769-Immunohistochemistry-Paraffin-NBP2-44495-img0004.jpg)