| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88706-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2519SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56343SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76910-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56649SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24669SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50045SS | |||
![Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human prostate shows moderate to strong positivity in nuclear speckles in glandular cells.](http://images.novusbio.com/fullsize/SON-Antibody-Immunohistochemistry-Paraffin-NBP1-88706-img0010.jpg "Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human prostate shows moderate to strong positivity in nuclear speckles in glandular cells.")
![Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human cerebellum shows moderate to strong positivity in nuclear speckles in Purkinje cells.](http://images.novusbio.com/fullsize/SON-Antibody-Immunohistochemistry-Paraffin-NBP1-88706-img0008.jpg)
![Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human small intestine shows moderate to strong positivity in nuclear speckles in glandular cells.](http://images.novusbio.com/fullsize/SON-Antibody-Immunohistochemistry-Paraffin-NBP1-88706-img0009.jpg)
![Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human tonsil shows moderate to strong positivity in nuclear speckles in germinal center cells.](http://images.novusbio.com/fullsize/SON-Antibody-Immunohistochemistry-Paraffin-NBP1-88706-img0011.jpg)
![Western Blot: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Analysis was performed on whole cell extracts (30 ug lysate) of A431 (Lane 1), HeLa (Lane 2), Jurkat (Lane 3), HT29 (Lane 4) and COS-7 (Lane 5). The blots were probed with Anti-TIMP1 Mouse .](http://images.novusbio.com/fullsize/TIMP-1-Antibody-F31-P2-A5-Western-Blot-NB100-74551-img0009.jpg "Western Blot: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Analysis was performed on whole cell extracts (30 ug lysate) of A431 (Lane 1), HeLa (Lane 2), Jurkat (Lane 3), HT29 (Lane 4) and COS-7 (Lane 5). The blots were probed with Anti-TIMP1 Mouse .")
![Immunocytochemistry/Immunofluorescence: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TIMP1 Monclonal Antibody (F31 P2 A5) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.](http://images.novusbio.com/fullsize/TIMP-1-Antibody-F31-P2-A5-Immunocytochemistry-Immunofluorescence-NB100-74551-img0005.jpg)
![Immunohistochemistry-Paraffin: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Normal biopsies of deparaffinized Human prostate tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/TIMP-1-Antibody-F31-P2-A5-Immunohistochemistry-Paraffin-NB100-74551-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Analysis of TIMP1 (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TIMP1 Monclonal Antibody (F31 P2 A5) at a dilution of 1:100 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.](http://images.novusbio.com/fullsize/TIMP-1-Antibody-F31-P2-A5-Immunocytochemistry-Immunofluorescence-NB100-74551-img0004.jpg)
![Immunohistochemistry-Paraffin: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Cancer biopsies of deparaffinized Human breast carcinoma tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/TIMP-1-Antibody-F31-P2-A5-Immunohistochemistry-Paraffin-NB100-74551-img0007.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0008.jpg "Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.")
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunohistochemistry-Paraffin-NBP2-22203-img0004.jpg)
![Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Flow-Cytometry-NBP2-22203-img0001.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Analysis using MAPK3 mAb against human ERK1 (AA: 9-143) recombinant protein. (Expected MW is 40.8 kDa)](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0006.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis using ERK1 mouse mAb against Hela (1), Jurkat (2), RAW264.7 (3), HEK293 (4), K562 (5), NIH/3T3 (6), Cos7 (7) and PC-12 (8) cell lysate.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0007.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of paraffin-embedded bladder cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunohistochemistry-Paraffin-NBP2-22203-img0003.jpg)
![ELISA: ERK1 Antibody (1E5) [NBP2-22203] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-ELISA-NBP2-22203-img0005.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - CRYAB antibody was tested in SHSH5Y cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2519-img0004.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Detection of CryAB using NB 100-2519 (0.5ug/ml). Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB100-2519-img0005.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Chk1 Antibody [NB100-464] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Chk1-Antibody-Immunohistochemistry-Paraffin-NB100-464-img0029.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-464] - Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-464-img0032.jpg)
![Immunoprecipitation: Chk1 Antibody [NB100-464] - Detection of human Chk1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 (lot 2) used for IP at 6 ug/mg lysate. Chk1 was also immunoprecipitated by a previous lot (lot 1) of this antibody. For blotting immunoprecipitated Chk1, NB100-464 (lot 2) was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Chk1-Antibody-Immunoprecipitation-NB100-464-img0033.jpg)
![Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells.(G) WB for indicated proteins in clones indicated on the top. Darker exposure of ORC2 blots is shown at the bottom. Ponceau S stains all proteins on the blot & also indicates equal loading of lanes. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-464_rabbit-polyclonal-chk1-antibody-310202415371972.jpg)
![Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC1 in HCT116 p53-/- cells.(A) Strategy for insertion of a blasticidin gene & poly A site after first methionine of ORC1 in the second exon. (B) PCR on genomic DNA of indicated clones. WT: HCT116 p53-/- & ORC1+/+. ORC1 Knockout clones, B14, B48 & BP32 have an insert on both alleles of ORC1 as indicated by the absence of 0.6 kb PCR product. (C) Western blot for indicated proteins in clones indicated on the top. Darker exposure of the ORC1 blots is shown at the bottom. Ponceau S stains all proteins on the blot & also indicates equal loading of lanes. (D) Immunoblot of soluble & chromatin-associated proteins in the clones indicated at the top. For each panel, all the lanes are from the same blot & exposure. (E) Input cell lysate & immunoprecipitates of ORC1 immunoblotted for ORC1. Darker exposure of the top blots is shown in the middle. Tubulin in the cell lysate or the IgG band in the immunoprecipitate serves as loading control.DOI://dx.doi.org/10.7554/eLife.19084.005 Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-464_rabbit-polyclonal-chk1-antibody-310202415525253.jpg)
![Western Blot: HDAC6 Antibody [NB100-56343] - Analysis of HDAC-6 in NIH-3T3 cell lysate with this antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NB100-56343-img0002.jpg "Western Blot: HDAC6 Antibody [NB100-56343] - Analysis of HDAC-6 in NIH-3T3 cell lysate with this antibody.")
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NB100-56343] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-HDAC6 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56343-img0004.jpg)
![Simple Western: HDAC6 Antibody [NB100-56343] - Lane view shows a specific band for HDAC6 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Simple-Western-NB100-56343-img0003.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NBP1-32075-img0025.jpg "Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500")
![Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-32075] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PCNA protein stained by PCNA antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32075-img0026.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody diluted at a dilution of 1:2500.](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NBP1-32075-img0020.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug NIH-3T3 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NBP1-32075-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-32075] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PCNA protein stained by PCNA antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32075-img0014.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Mouse testis. PCNA antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-32075-img0013.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Human colon. PCNA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-32075-img0016.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Human cervical carcinoma. PCNA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-32075-img0017.jpg)
![Immunoprecipitation: PCNA Antibody [NBP1-32075] - Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 ug of PCBP2 antibody. Western blot analysis was performed using PCBP2 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunoprecipitation-NBP1-32075-img0023.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody (NBP1-32075) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32075_rabbit-polyclonal-pcna-antibody-910202420131282.jpg)
![Western Blot: APP Antibody [NBP1-76910] - Analysis of APP in mouse brain tissue lysate with APP antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/APP-Antibody-Western-Blot-NBP1-76910-img0012.jpg "Western Blot: APP Antibody [NBP1-76910] - Analysis of APP in mouse brain tissue lysate with APP antibody at (A) 0.5 and (B) 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: APP Antibody - BSA Free [NBP1-76910] - Immunofluorescence of APP in Rat Brain cells with APP antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-2711202419451253.jpg)
![Immunocytochemistry/ Immunofluorescence: APP Antibody - BSA Free [NBP1-76910] - Immunofluorescence of APP in mouse brain tissue with APP Antibody at 20 ug/mL. <br><br>Green: APP antibody <br> Red: Phylloidin staining<br> Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-2711202419541571.gif)
![Immunohistochemistry: APP Antibody - BSA Free [NBP1-76910] - Immunohistochemistry of APP in mouse brain tissue with APP Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-2711202419251561.gif)
![Immunohistochemistry: APP Antibody - BSA Free [NBP1-76910] - Immunohistochemical staining of rat brain using APP antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-271120241932669.jpg)
![Western Blot: Lamin A + C Antibody [NB100-56649] - Analysis of human Lamin A + C in 15 ug of HeLa cell lysate using NB100-56649 at 1:1000.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NB100-56649-img0003.jpg "Western Blot: Lamin A + C Antibody [NB100-56649] - Analysis of human Lamin A + C in 15 ug of HeLa cell lysate using NB100-56649 at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NB100-56649] - Lamin A/C antibody was tested in A431 cells at 1:100 against DyLight 488 (Green). Alpha tubulin and nuclei were counterstained against DyLight 568 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56649-img0005.jpg)
![Flow (Intracellular): Lamin A + C Antibody [NB100-56649] - Intracellular staining of HeLa cells (1 x 10^6 cells/mL) with Lamin A+C antibody (orange) stained at 1:1000. Detected with a GtxRb DyLight 488 secondary. Shown with the secondary only control (blue).](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Flow-Intracellular-NB100-56649-img0004.jpg)
![Western Blot: DLL3 Antibody [NBP2-24669] - Analysis of DLL3 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide and 3) T98G cell lysate using DLL3 antibody at 3 and 5 ug/mL respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/DLL3-Antibody-Western-Blot-NBP2-24669-img0001.jpg "Western Blot: DLL3 Antibody [NBP2-24669] - Analysis of DLL3 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide and 3) T98G cell lysate using DLL3 antibody at 3 and 5 ug/mL respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: DLL3 Antibody [NBP2-24669] - Human brain tissue probed with an isotype control (top) and DLL3 antibody (bottom) at 5 ug/mL.](http://images.novusbio.com/fullsize/DLL3-Antibody-Immunohistochemistry-Paraffin-NBP2-24669-img0002.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19437] - Human colon cancer. mtTFA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP2-19437-img0007.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19437] - Human esophagus cancer. mtTFA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP2-19437-img0006.jpg)
![Western Blot: mtTFA Antibody [NBP2-19437] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with mtTFA antibody (NBP2-19437) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19437_rabbit-polyclonal-mttfa-antibody-910202420115071.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: mtTFA Antibody [NBP2-19437] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19437_rabbit-polyclonal-mttfa-antibody-1610202419414569.jpg)
![mtTFA antibody detects mtTFA protein at mito by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: mtTFA stained by mtTFA antibody (NBP2-19437) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19437_rabbit-polyclonal-mttfa-antibody-immunocytochemistry-immunofluorescence-261120251831541.jpg)
![Western Blot: ALDH1L1 Antibody (4A12) [NBP2-50045] - Analysis of rat and mouse tissue lysates using mouse ALDH1L1 mAb, dilution 1:5000 (Green): [1] protein standard (Red), rat tissue lysates: [2] heart, [3] liver, [4] kidney, [5] lung, [6] brain, and [7] spinal cord; Also mouse tissue lysates: [8] brain, and [9] spinal cord. The band at 100kDa corresponds to ALDH1L1 protein. In line with published work, ALDH1L1 is a major protein of liver and CNS tissue.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-4A12-Western-Blot-NBP2-50045-img0006.jpg "Western Blot: ALDH1L1 Antibody (4A12) [NBP2-50045] - Analysis of rat and mouse tissue lysates using mouse ALDH1L1 mAb, dilution 1:5000 (Green): [1] protein standard (Red), rat tissue lysates: [2] heart, [3] liver, [4] kidney, [5] lung, [6] brain, and [7] spinal cord; Also mouse tissue lysates: [8] brain, and [9] spinal cord. The band at 100kDa corresponds to ALDH1L1 protein. In line with published work, ALDH1L1 is a major protein of liver and CNS tissue.")
![Immunocytochemistry/Immunofluorescence: ALDH1L1 Antibody (4A12) [NBP2-50045] - Analysis of mixed cortical neuron-glial cell culture from an E20 rat stained with mouse ALDH1L1 mAb, dilution 1:1,000 (Green), and costained with chicken Vimentin pAb, dilution 1:5000 (Red). DAPI staining of nuclear DNA (Blue). The ALDH1L1 antibody produces strong cytoplasmic staining of astrocytic glial cells, while the Vimentin antibody labels the intermediate filament cytoskeleton in fibroblasts and other glial cells. Some cells, presumably developing astrocytes, are stained by both ALDH1L1 and vimentin and so appear yellow.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-4A12-Immunocytochemistry-Immunofluorescence-NBP2-50045-img0005.jpg)