| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33321-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47836-0.1mg | |||
| IHC-Prediluted | NBP2-48321 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47837-0.1mg | |||
| IHC-Prediluted | NBP2-48322 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42210SS | |||
| Azide and BSA Free | NBP2-80739 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56339SS | |||
![Western Blot: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Western Blot Analysis of human MCF-7 cell lysate using ER alpha/NR3A1 Antibody (ER506).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Western-Blot-NBP2-33320-img0017.jpg "Western Blot: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Western Blot Analysis of human MCF-7 cell lysate using ER alpha/NR3A1 Antibody (ER506).")
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Formalin-fixed, paraffin-embedded human Endometrium stained with ER alpha/NR3A1 Antibody (ER506).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Immunohistochemistry-Paraffin-NBP2-33320-img0015.jpg)
![Flow Cytometry: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Flow Cytometry for human ER-alpha on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER alpha/NR3A1 antibody (ER506).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Flow-Cytometry-NBP2-33320-img0018.jpg)
![Simple Western: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Simple Western lane view shows a specific band for Estrogen Receptor in 0.2 mg/ml of MCF-7 (left) and T47D (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Simple-Western-NBP2-33320-img0007.jpg)
![Simple Western: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Electropherogram image of the corresponding Simple Western lane. Estrogen Receptor antibody was used at 10 ug/ml dilution of MCF-7 and T47D lysates(s) respectively.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Simple-Western-NBP2-33320-img0009.jpg)
![Protein Array: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ER alpha/NR3A1 Antibody (ER506). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Protein-Array-NBP2-33320-img0016.jpg)
![Western Blot: Ornithine Decarboxylase Antibody (ODC1/486) [NBP2-44734] - Western Blot Analysis of PC3 cell lysate usingOrnithine Decarboxylase antibody (ODC1/486).](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-486-Western-Blot-NBP2-44734-img0005.jpg "Western Blot: Ornithine Decarboxylase Antibody (ODC1/486) [NBP2-44734] - Western Blot Analysis of PC3 cell lysate usingOrnithine Decarboxylase antibody (ODC1/486).")
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/486) [NBP2-44734] - Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with Ornithine Decarboxylase Antibody (ODC1/486).](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-486-Immunohistochemistry-Paraffin-NBP2-44734-img0004.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/486) [NBP2-44734] - Human Pancreas stained with ODC1 Monoclonal Antibody (ODC1/486)](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-486-Immunohistochemistry-Paraffin-NBP2-44734-img0001.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/486) [NBP2-44734] - Rat Pancreas stained with ODC1 Monoclonal Antibody (ODC1/486)](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-486-Immunohistochemistry-Paraffin-NBP2-44734-img0002.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/486) [NBP2-44734] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with Ornithine Decarboxylase Antibody (ODC1/486).](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-486-Immunohistochemistry-Paraffin-NBP2-44734-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - IF staining of LNCap cells using AF488 labeled Ornithine Decarboxylase Antibody (ODC1/487). (Green). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-487-Immunocytochemistry-Immunofluorescence-NBP2-44735-img0005.jpg "Immunocytochemistry/Immunofluorescence: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - IF staining of LNCap cells using AF488 labeled Ornithine Decarboxylase Antibody (ODC1/487). (Green). DAPI was used to stain the cell nuclei (blue).")
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with Ornithine Decarboxylase Antibody (ODC1/487)](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-487-Immunohistochemistry-Paraffin-NBP2-44735-img0007.jpg)
![Flow Cytometry: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - An intracellular stain was performed on K562 cells with Ornithine Decarboxylase [ODC1/487] Antibody NBP2-47837AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-487-Flow-Cytometry-NBP2-44735-img0004.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - Rat Pancreas stained with ODC1 Monoclonal Antibody (ODC1/487)](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-487-Immunohistochemistry-Paraffin-NBP2-44735-img0001.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with Ornithine Decarboxylase Antibody (ODC1/487)](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-487-Immunohistochemistry-Paraffin-NBP2-44735-img0006.jpg)
![In vitro assay: TNFSF4 Protein [NBP2-26577] - Demonstration in vitro of the potency of Fc-OX40L in comparison to an agonist antibody OX86, with anti-CD3 alone serving as a negative control for co-stimulation.](http://images.novusbio.com/fullsize/TNFSF4-Protein-In-vitro-assay-NBP2-26577-img0001.jpg "In vitro assay: TNFSF4 Protein [NBP2-26577] - Demonstration in vitro of the potency of Fc-OX40L in comparison to an agonist antibody OX86, with anti-CD3 alone serving as a negative control for co-stimulation.")
![SDS-Page: TNFSF4 Protein [NBP2-26577] - Electrophoretic analysis of Fc-mOX40L using Coomassie blue stained 4-20% reducing SDS-PAGE.](http://images.novusbio.com/fullsize/TNFSF4-Protein-SDS-Page-NBP2-26577-img0002.jpg)
![SDS-Page: Recombinant Mouse OX40 Ligand/TNFSF4 Protein [NBP2-26577] - Electrophoretic analysis of Fc-mOX40L using Coomassie blue stained 4-15% reducing SDS-PAGE.](http://images.novusbio.com/fullsize/Recombinant-Mouse-OX40-Ligand-TNFSF4-Protein-SDS-Page-NBP2-26577-img0004.jpg)
![Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - WB analysis of lysates from (A) Human Embryonic Kidney 293 / HEK293 and (B) Human Embryonic carcinoma NCCIT cell lines using Flt-3/Flk-2/CD135 antibody (clone 7E8.2C8) at 2ug/ml concentration. The antibody detected single specific bands in both samples at the expected molecular weight position.](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7E8-2C8-Western-Blot-NBP2-42210-img0001.jpg "Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - WB analysis of lysates from (A) Human Embryonic Kidney 293 / HEK293 and (B) Human Embryonic carcinoma NCCIT cell lines using Flt-3/Flk-2/CD135 antibody (clone 7E8.2C8) at 2ug/ml concentration. The antibody detected single specific bands in both samples at the expected molecular weight position.")
![Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - IHC analysis of a formalin fixed paraffin embedded tissue section of human liver cancer using Flt-3/Flk-2/CD135 antibody (clone 7E2.2C8) at 15 ug/ml concentration. The antibody generated specific cytoplasmic immunostaining of FLT3 in the cancerous cells while the tumor stroma was largely negative for the staining.](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7E8-2C8-Immunohistochemistry-Paraffin-NBP2-42210-img0003.jpg)
![Flow Cytometry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - HEK293 cells were stained (surface) with Flt-3/Flk-2/CD135 antibody (clone: 7E8.2C8; red) or isotype control (mouse IgG2 lambda; blue) and positive staining observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7E8-2C8-Flow-Cytometry-NBP2-42210-img0002.jpg)
![Western Blot: Mre11 Antibody [NB100-56339] - Western blot analysis of 293 lysate blotted with MRE11 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Mre11-Antibody-Western-Blot-NB100-56339-img0003.jpg "Western Blot: Mre11 Antibody [NB100-56339] - Western blot analysis of 293 lysate blotted with MRE11 antibody at 2 ug/ml.")
![Immunohistochemistry-Paraffin: Mre11 Antibody [NB100-56339] - Paraffin-embedded human testis using MRE11 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/Mre11-Antibody-Immunohistochemistry-Paraffin-NB100-56339-img0002.jpg)
![Western Blot: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PMN (peripheral blood mononuclear cells isolated from buffycoat; denatured, reduced) using Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum at 1: 500 dilution; blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Western-Blot-NBP1-25966-img0017.jpg "Western Blot: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PMN (peripheral blood mononuclear cells isolated from buffycoat; denatured, reduced) using Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum at 1: 500 dilution; blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.")
![Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10 min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 min at RT. Excess of blocking solution was removed and cells were then incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-25966-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20min at RT. Excess blocking solution was removed and cells were incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-25966-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20min at RT. Excess of blocking solution was removed and cells were then incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-25966-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10 min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 min at RT. Excess of blocking solution was removed and cells were then incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-25966-img0020.jpg)
![Western Blot: RIOK3 Antibody (4A12-1D2) [H00008780-M01] - RIOK3 monoclonal antibody (M01), clone 4A12-1D2. Analysis of RIOK3 expression in MCF-7.](http://images.novusbio.com/fullsize/RIOK3-Antibody-4A12-1D2-Western-Blot-H00008780-M01-img0005.jpg "Western Blot: RIOK3 Antibody (4A12-1D2) [H00008780-M01] - RIOK3 monoclonal antibody (M01), clone 4A12-1D2. Analysis of RIOK3 expression in MCF-7.")
![Western Blot: RIOK3 Antibody (4A12-1D2) [H00008780-M01] - Western Blot analysis of RIOK3 expression in Hela NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/RIOK3-Antibody-4A12-1D2-Western-Blot-H00008780-M01-img0001.jpg)
![Western Blot: RIOK3 Antibody (4A12-1D2) [H00008780-M01] - Analysis of RIOK3 expression in transfected 293T cell line by RIOK3 monoclonal antibody (M01), clone 4A12-1D2.Lane 1: RIOK3 transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RIOK3-Antibody-4A12-1D2-Western-Blot-H00008780-M01-img0006.jpg)
![Immunoprecipitation: RIOK3 Antibody (4A12-1D2) [H00008780-M01] - Analysis of RIOK3 transfected lysate using anti-RIOK3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RIOK3 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/RIOK3-Antibody-4A12-1D2-Immunoprecipitation-H00008780-M01-img0003.jpg)
![Sandwich ELISA: RIOK3 Antibody (4A12-1D2) [H00008780-M01] - Detection limit for recombinant GST tagged RIOK3 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RIOK3-Antibody-4A12-1D2-Sandwich-ELISA-H00008780-M01-img0004.jpg)
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Membrane attack complex-induced and endosome-dependent stabilization of ZFYVE21. Human umbilical vein endothelial cells (HUVECs) were treated with panel reactive antibody (PRA) sera (25% v/v in gelatin veronal buffer) (a). HUVECs were treated with MG132 (25uM). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-10041-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-H00079038-B01P-img0005.jpg "Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Membrane attack complex-induced and endosome-dependent stabilization of ZFYVE21. Human umbilical vein endothelial cells (HUVECs) were treated with panel reactive antibody (PRA) sera (25% v/v in gelatin veronal buffer) (a). HUVECs were treated with MG132 (25uM). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-10041-2), licensed under a CC-BY license.")
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Analysis of ZFYVE21 expression in transfected 293T cell line by ZFYVE21 polyclonal antibody. Lane 1: ZFYVE21 transfected lysate(25.74 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-H00079038-B01P-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Correlations of ZFYVE21 and complement in patient tissues. Western blots of human umbilical vein endothelial cells treated with either panel reactive antibody (30min, 25% v/v in gelatin veronal buffer) or LIGHT (12h, 50ng/mL in EGM2 media, a). Kidney biopsies from renal transplant patients (n=12) were stained as indicated. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-10041-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-H00079038-B01P-img0003.jpg)
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Recombinant FLAG-tagged ZFYVE21 (1ug) and/or recombinant SMURF2 (1ug) protein was incubated for 30min at room temperature and analyzed by western blot. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-10041-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-H00079038-B01P-img0004.jpg)
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Correlations of ZFYVE21 & complement in patient tissues. Western blots of human umbilical vein endothelial cells treated with either panel reactive antibody (30 min, 25% v/v in gelatin veronal buffer) or LIGHT (12 h, 50 ng/mL in EGM2 media, a). Kidney biopsies from renal transplant patients (n = 12) were stained as indicated (b). Synovial biopsies from osteoarthritis (n = 11), low-titer rheumatoid factor antibody rheumatoid arthritis (RF RA; n = 8), & high-titer RF RA (n = 8) were stained as indicated (c–e) & spatial correlations between ZFVYE21 or nuclear factor-inducing kinase (NIK) with endothelial cells (Ulex, f) & membrane attack complex (polyC9, g) were assessed using the computer software (scale bar: 200 μm). ****p < 1 × 10−5, Student’s t test. CD45 abundance was assessed (h) & correlated with NIK staining (i) via blinded scoring from three independent observers. *p < 0.05, **p < 0.001. Western blotting was repeated three times. Analysis of variance statistical analyses were performed. Representative data shown *p < 0.05, **p < 0.001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31113953), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00079038-b01p_mouse-polyclonal-zfyve21-antibody-31020241548367.jpg)
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - SMURF2-dependent degradation of vesicular PTEN. PTEN co-immunoprecipitations (co-IPs) were performed on human umbilical vein endothelial cells (HUVECs) treated for 30 min with panel reactive antibody (PRA) sera & blotted as indicated (a). Rab5 co-IPs were performed on PRA-treated HUVECs transfected with control or SMURF2 siRNA (b). Western blot analysis of whole-cell lysates of HUVECs transiently transfected with SMURF2 WT, SMURF2 DN, control siRNA, & SMURF2 siRNA (c). HUVECs transfected with control, SMURF2, or ZFYVE21 siRNA were treated with PRA & Rab5 co-IPs were performed (d). Recombinant FLAG-tagged ZFYVE21 (1 μg) and/or recombinant SMURF2 (1 μg) protein was incubated for 30 min at room temperature & analyzed by western blot (e). In vitro kinase assays were performed using various E2 ubiquitin ligases with SMURF2 protein (1 μg) as the E3 ubiquitin ligase & PTEN (1 μg) as substrate (f). In vitro kinase assays were performed using isolated SMURF2 WT or SMURF2 DN as E3 ubiquitin ligases, UbcH5c as the E2 ubiquitin ligase, & PTEN as substrate as indicated (g). HUVECs were pretreated with MG132 for 4 h or chloroquine for 30 min prior to PRA treatment at the indicated times prior to Rab5 co-IP & western blot analysis (h). Western blot analyses were repeated two to four times. Confocal microscopic studies analyzed ≥4 cells per group & was repeated three times. In vitro kinase assays were repeated twice. Representative data shown Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31113953), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00079038-b01p_mouse-polyclonal-zfyve21-antibody-310202415485117.jpg)
![Immunocytochemistry/Immunofluorescence: KIFC1 Antibody (2B9) [H00003833-M01] - Analysis of monoclonal antibody to KIFC1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/KIFC1-Antibody-2B9-Immunocytochemistry-Immunofluorescence-H00003833-M01-img0005.jpg "Immunocytochemistry/Immunofluorescence: KIFC1 Antibody (2B9) [H00003833-M01] - Analysis of monoclonal antibody to KIFC1 on HeLa cell. Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: KIFC1 Antibody (2B9) [H00003833-M01] - Analysis of monoclonal antibody to KIFC1 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/KIFC1-Antibody-2B9-Immunohistochemistry-Paraffin-H00003833-M01-img0007.jpg)
![ELISA: KIFC1 Antibody (2B9) [H00003833-M01] - Detection limit for recombinant GST tagged KIFC1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/KIFC1-Antibody-2B9-ELISA-H00003833-M01-img0009.jpg)
![Western Blot: BRAF35 Antibody (1F6) [H00010362-M01] - HMG20B monoclonal antibody (M01), clone IF6 Analysis of HMG20B expression in HeLa.](http://images.novusbio.com/fullsize/BRAF35-Antibody-1F6-Western-Blot-H00010362-M01-img0010.jpg "Western Blot: BRAF35 Antibody (1F6) [H00010362-M01] - HMG20B monoclonal antibody (M01), clone IF6 Analysis of HMG20B expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: BRAF35 Antibody (1F6) [H00010362-M01] - Analysis of monoclonal antibody to HMG20B on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/BRAF35-Antibody-1F6-Immunocytochemistry-Immunofluorescence-H00010362-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: BRAF35 Antibody (1F6) [H00010362-M01] - Analysis of monoclonal antibody to HMG20B on formalin-fixed paraffin-embedded human lung cancer. Antibody concentration 1.8 ug/ml.](http://images.novusbio.com/fullsize/BRAF35-Antibody-1F6-Immunohistochemistry-Paraffin-H00010362-M01-img0009.jpg)
![Western Blot: BRAF35 Antibody (1F6) [H00010362-M01] - Western blot analysis of HMG20B in extracts from 293T, HeLa and A549 cell using anti-HMG20B monoclonal antibody ( Cat # L013V1 ).](http://images.novusbio.com/fullsize/BRAF35-Antibody-1F6-Western-Blot-H00010362-M01-img0001.jpg)
![Western Blot: BRAF35 Antibody (1F6) [H00010362-M01] - Analysis of HMG20B in extracts from 293T, HeLa and A549 cell using anti-HMG20B monoclonal antibody.](http://images.novusbio.com/fullsize/BRAF35-Antibody-1F6-Western-Blot-H00010362-M01-img0011.jpg)