| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54551-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44974 | |||
| Azide and BSA Free | NBP2-34751-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33240 | |||
![Western Blot: Nucleophosmin Antibody (FC82291) [NB600-1030] - Monoclonal Anti-B23 antibody produced in mouse](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-FC82291-Western-Blot-NB600-1030-img0001.jpg "Western Blot: Nucleophosmin Antibody (FC82291) [NB600-1030] - Monoclonal Anti-B23 antibody produced in mouse")
![Immunofluorescence: NPM1 Antibody (FC82291) [NB600-1030] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:500. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/NPM1-Antibody-FC82291-Immunofluorescence-NB600-1030-img0002.jpg)
![Immunoblotting: NPM1 Antibody (FC82291) [NB600-1030] - Cell line lysates were separated on SDS-PAGE and probed with 0.5 ug/mL Monoclonal Anti-B23 Clone: FC82291 Lanes 1. HeLa 2. JURKAT 3. COS7 4. NIH-3T3 5. RAT2 6. CHO 7. MDBK 8. MDCK](http://images.novusbio.com/fullsize/NPM1-Antibody-FC82291-Immunoblotting-NB600-1030-img0008.jpg)
![Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214] - Samples: Whole cell lysate (15 ug) from HEK293T, K-562, Jurkat, HeLa, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody NB100-214 (lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Western-Blot-NB100-214-img0003.jpg "Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214] - Samples: Whole cell lysate (15 ug) from HEK293T, K-562, Jurkat, HeLa, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody NB100-214 (lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214] - Samples: Whole cell lysate (50 ug) from TCMK-1 and BIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody NB100-214 (lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Western-Blot-NB100-214-img0002.jpg)
![Immunoprecipitation: Blooms Syndrome Protein Blm Antibody [NB100-214] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BLM antibody (NB100-214, lot 4) used for IP at 6 ug per reaction. BLM was also immunoprecipitated by a previous lot of this antibody (lot 3). For blotting immunoprecipitated BLM, NB100-214 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Immunoprecipitation-NB100-214-img0001.jpg)
![Western Blot: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Western Blot Analysis (A) Recombinant Protein (B) human Stomach lysate Using E-Cadherin Monoclonal Antibody (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Western-Blot-NBP2-53354-img0006.jpg "Western Blot: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Western Blot Analysis (A) Recombinant Protein (B) human Stomach lysate Using E-Cadherin Monoclonal Antibody (CDH1/1525).")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Confocal Immunofluorescence of MCF-7 cells E-Cadherin Antibody (CDH1/1525). labeled with CF488 (Green); Red Dot is used to label the nuclei.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunocytochemistry-Immunofluorescence-NBP2-53354-img0007.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-fixed, paraffin-embedded human breast adenocarcinoma stained with E-Cadherin Mouse Monoclonal Antibody (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0009.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with E-Cadherin Monoclonal Antibody (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0001.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-fixed, paraffin-embedded human Skin stained with E-Cadherin Monoclonal Antibody (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0002.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-paraffin human Placenta stained with E-Cadherin MAb (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0004.jpg)
![Protein Array: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Analysis of Protein Array containing more than 19,000 full-length human proteins using E-Cadherin Antibody (CDH1/1525).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Protein-Array-NBP2-53354-img0008.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Human breast cancer tissue stained fro E-cadherin (white) and counterstained with DAPI (blue). Alexa Fluor 750 version of antibody used. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-53354_mouse-monoclonal-e-cadherin-antibody-cdh1-1525-17720241044616.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Western-Blot-NB100-68210-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209.")
![<b>Independent Antibodies Validation. </b>Simple Western: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. Validation of interactions between GFP-E2F4 and DP-1, Host Cell Factor 1/HCFC1, YEATS2, LIN54, and LIN9, by co-immunoprecipitation followed by immunoassay, in mouse ES cells and human RPE cells. Transcriptional activators (Host Cell Factor 1/HCFC1 and YEATS2) preferentially bind to E2F4 in mouse ES cells while members of the DREAM repressor complex (LIN54 and LIN9) bind preferentially to E2F4 in RPE cells. Molecular weights (kDa) are indicated on the left side (one experiment shown of at least two experiments) Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10901-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Simple-Western-NB100-68210-img0005.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. a Schematic representation of the affinity purification-mass spectrometry approach to identify the E2F4 interactome. b Silver stain of eluted fractions from RPE cells and mouse ES cells . Slices of gel were subjected to mass spectrometry, & the indicated protein bands were identified by analyzing the proteins enriched in each slice. c GO terms for cellular components enriched in the list of mouse ES cell-specific candidate interactors. d Validation of interactions between GFP-E2F4 & DP-1, HCFC1, YEATS2, LIN54, & LIN9, by co-immunoprecipitation followed by immunoassay, in mouse ES cells & human RPE cells. Transcriptional activators (HCFC1 & YEATS2) preferentially bind to E2F4 in mouse ES cells while members of the DREAM repressor complex (LIN54 & LIN9) bind preferentially to E2F4 in RPE cells. Molecular weights (kDa) are indicated on the left side (one experiment shown of at least two experiments) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31270324), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68210_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415541972.jpg)
![Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody [NBP2-16814] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hexokinase II stained by Hexokinase II antibody diluted at 1:2000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16814-img0017.jpg "Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody [NBP2-16814] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hexokinase II stained by Hexokinase II antibody diluted at 1:2000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse muscle. Hexokinase II stained by Hexokinase II antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0022.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - U87 xenograft. Hexokinase II antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Hexokinase-II-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0003.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse muscle. Hexokinase II antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0005.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat heart. Hexokinase II antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0007.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat stomach. Hexokinase II antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0008.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat colon. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0012.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat kidney. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0013.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse heart. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0014.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat muscle. Hexokinase II stained by Hexokinase II antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0015.jpg)
![Western Blot: HSP27 Antibody (SPM252) [NBP2-32865] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (SPM252).](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Western-Blot-NBP2-32865-img0005.jpg "Western Blot: HSP27 Antibody (SPM252) [NBP2-32865] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (SPM252).")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (SPM252) [NBP2-32865] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunocytochemistry-Immunofluorescence-NBP2-32865-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 Mouse Monoclonal Antibody (SPM252).](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunohistochemistry-Paraffin-NBP2-32865-img0006.jpg)
![Flow Cytometry: HSP27 Antibody (SPM252) [NBP2-32865] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (SPM252).followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Flow-Cytometry-NBP2-32865-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-paraffin breast carcinoma (20X) stained with HSP27 MAb (SPM252)](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunohistochemistry-Paraffin-NBP2-32865-img0002.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-paraffin breast carcinoma (10X) stained with HSP27 MAb (SPM252))](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunohistochemistry-Paraffin-NBP2-32865-img0001.jpg)
![Immunohistochemistry: Plasminogen Antibody [NBP2-19859] - Expression of plasminogen/plasmin (NBP2-19859) in normal and PCOS mouse ovaries. Representative histological images are from normal (control) (Ai, Aiii) or PCOS ovaries (Aii, Aiv) stained with an anti-plasminogen/plasmin antibody. The scale bar represents 500 um for Ai, Aii and 100 um for Aiii, Aiv. Right: Top: Negative control for plasminogen/plasmin on mouse liver; Middle: Graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for plasminogen/plasmin and plasminogen alone between normal and PCOS ovaries for 6 replicates of each experimental group (mean +/- SD) and Bottom: Negative control for plasminogen alone on mouse liver Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/s12958-019-0472-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Immunohistochemistry-NBP2-19859-img0013.jpg "Immunohistochemistry: Plasminogen Antibody [NBP2-19859] - Expression of plasminogen/plasmin (NBP2-19859) in normal and PCOS mouse ovaries. Representative histological images are from normal (control) (Ai, Aiii) or PCOS ovaries (Aii, Aiv) stained with an anti-plasminogen/plasmin antibody. The scale bar represents 500 um for Ai, Aii and 100 um for Aiii, Aiv. Right: Top: Negative control for plasminogen/plasmin on mouse liver; Middle: Graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for plasminogen/plasmin and plasminogen alone between normal and PCOS ovaries for 6 replicates of each experimental group (mean +/- SD) and Bottom: Negative control for plasminogen alone on mouse liver Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/s12958-019-0472-0) licensed under a CC-BY license.")
![Western Blot: Plasminogen Antibody [NBP2-19859] - Plasminogen antibody detects PLG protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract7. 5 % SDS-PAGEPlasminogen antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NBP2-19859-img0001.jpg)
![Western Blot: Plasminogen Antibody [NBP2-19859] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NBP2-19859-img0002.jpg)
![Western Blot: Plasminogen Antibody [NBP2-19859] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NBP2-19859-img0003.jpg)
![Immunohistochemistry-Paraffin: Plasminogen Antibody [NBP2-19859] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Immunohistochemistry-Paraffin-NBP2-19859-img0004.jpg)
![Immunohistochemistry-Paraffin: Plasminogen Antibody [NBP2-19859] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Immunohistochemistry-Paraffin-NBP2-19859-img0005.jpg)
![Immunohistochemistry-Paraffin: Plasminogen Antibody [NBP2-19859] - Paraffin-embedded rat prostate Plasminogen antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Immunohistochemistry-Paraffin-NBP2-19859-img0008.jpg)
![Western Blot: Lamin A + C Antibody [NBP2-25153] - Stripe blot of crude HeLa cell extract. Note two strong and clean bands at 74kDa and 65kDa, corresponding to Lamin A and C.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NBP2-25153-img0001.jpg "Western Blot: Lamin A + C Antibody [NBP2-25153] - Stripe blot of crude HeLa cell extract. Note two strong and clean bands at 74kDa and 65kDa, corresponding to Lamin A and C.")
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-25153] - HeLa cells stained with NBP2-25153 (red) counterstained with monoclonal antibody to Hsp27, NBP2-25149 (green) and DNA (blue). NBP2-25153 reveals strong nuclear lamina staining, while NBP2-25149 reveals strong cytoplasmic staining. Since both DNA (blue) and Lamin A + C (red) are associated with the nuclear compartment, this region appears crimson in this image.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25153-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: APC Antibody [NBP2-15422] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: APC protein stained by APC antibody [C3], C-term diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody NBP2-43837 diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/APC-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15422-img0005.jpg "Immunocytochemistry/Immunofluorescence: APC Antibody [NBP2-15422] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: APC protein stained by APC antibody [C3], C-term diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody NBP2-43837 diluted at 1:1000. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: APC Antibody [NBP2-15422] - Human colon cancer. APC stained by APC antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, 20 min.](http://images.novusbio.com/fullsize/APC-Antibody-Immunohistochemistry-Paraffin-NBP2-15422-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: APC Antibody [NBP2-15422] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/APC-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15422-img0001.jpg)
![Immunohistochemistry-Paraffin: APC Antibody [NBP2-15422] - Rat colon. APC antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/APC-Antibody-Immunohistochemistry-Paraffin-NBP2-15422-img0006.jpg)
![Western Blot: APC Antibody [NBP2-15422] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with APC antibody [C3], C-term (NBP2-15422) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15422_rabbit-polyclonal-apc-antibody-910202420115060.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) unboiled HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with APC antibody [C3], C-term (NBP2-15422) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15422_rabbit-polyclonal-apc-antibody-western-blot-2611202518381518.jpg)
![Western Blot: Xrn1 Antibody [NB500-191] - Detection of human and mouse XRN1 by western blot. Samples:Whole cell lysate (15 ug) from GaMG, HEK293T, RKO, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN1 antibody NB500-191 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Xrn1-Antibody-Western-Blot-NB500-191-img0010.jpg "Western Blot: Xrn1 Antibody [NB500-191] - Detection of human and mouse XRN1 by western blot. Samples:Whole cell lysate (15 ug) from GaMG, HEK293T, RKO, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN1 antibody NB500-191 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation: Xrn1 Antibody [NB500-191] - Detection of human XRN1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XRN1 antibody NB500-191 (lot NB500-191-4) used for IP at 6 ug per reaction. XRN1 was also immunoprecipitated by a previous lot of this antibody (lot NB500-191-3). For blotting immunoprecipitated XRN1, NB500-191 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Xrn1-Antibody-Immunoprecipitation-NB500-191-img0011.jpg)
![Western Blot: RB1 Antibody (172C1094) - (cleaved) [NB100-56598] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and RB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human RB-1 Monoclonal Antibody (Catalog # NB100-56598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for RB-1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/RB1-Antibody-172C1094-cleaved-Knockdown-Validated-NB100-56598-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: RB1 Antibody (172C1094) - (cleaved) [NB100-56598] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and RB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human RB-1 Monoclonal Antibody (Catalog # NB100-56598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for RB-1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in HL60 cells were treated with camptothecin for 24 hours.](http://images.novusbio.com/fullsize/cleaved-Retinoblastoma-1-Antibody-172C1094-Western-Blot-NB100-56598-img0005.jpg)
![Immunohistochemistry: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in sections of primary human breast tumors. Image courtesy of Dr. Macleod, The Ben May Institute for Cancer Research, and The University of Chicago.](http://images.novusbio.com/fullsize/cleaved-Retinoblastoma-1-Antibody-172C1094-Immunohistochemistry-NB100-56598-img0004.jpg)
![Western Blot: MCM5 Antibody [NB100-79778] - Detection of Human MCM5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM5 antibody NB100-79778 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MCM5-Antibody-Western-Blot-NB100-79778-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM5 Antibody [NB100-79778] - Detection of Human MCM5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM5 antibody NB100-79778 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MCM5 Antibody [NB100-79778] - Human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- MCM5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM5-Antibody-Immunohistochemistry-NB100-79778-img0010.jpg)