| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97601SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75978SS | |||
| BSA Free | NBP2-75978 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86064-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-30626-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89033-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47698-0.1mg | |||
| IHC-Prediluted | NBP2-48132 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36567SS | |||
| Azide and BSA Free | NBP2-80653 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71602 | |||
![Immunohistochemistry-Frozen: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Staining of endogenous NLRP3/NALP3 in epithelial layer of human tonsil (frozen section) using NLRP3/NALP3 (human), mAb (Nalpy3-b). Method: 5uM frozen section of tissue are dried and fixed with aceton. Tissue is washed with PBS and incubated with NLRP3/NALP3 (human), mAb (Nalpy3-b).](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Nalpy3-b-Immunohistochemistry-Frozen-NBP1-97601-img0002.jpg "Immunohistochemistry-Frozen: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Staining of endogenous NLRP3/NALP3 in epithelial layer of human tonsil (frozen section) using NLRP3/NALP3 (human), mAb (Nalpy3-b). Method: 5uM frozen section of tissue are dried and fixed with aceton. Tissue is washed with PBS and incubated with NLRP3/NALP3 (human), mAb (Nalpy3-b).")
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Sterol-resistant SCAP overexpression induces the activation of NLRP3/NALP3 (NBP1-97601) infammasomes in VSMCs. Representative immunoblots for NLRP3/NALP3, Procaspase-1, cleaved caspase-1, IL-1 beta, and IL-18 in SCAP-overexpressing VSMCs. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-2552023153958.jpg)
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free [NBP1-97601] - Sterol-resistant SCAP overexpression in VSMCs causes inflammation & lipid deposition in the aortas of SCAPD443N mice. (A & B) Representative images of an aortic root stained with Oil Red O in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding. (C) Quantification of plaque areas in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding (n=5). (D) Plasma levels of TC, TG, LDL-C & HDL-C in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding (n=5). (E) mRNA expression of proin?ammatory cytokines (IL-1 beta , IL-6, IL-18, & TNF-alpha ) as measured by qRT-PCR (n=4). (F) mRNA expression of pro-chemokine cytokines (MCP-1, MIP-1 alpha , MIP-1 beta , & CX3CL1) as measured by qRT-PCR (n=4). (G) mRNA expression of ICAM-1 & VCAM-1 in aortas as measured by qRT-PCR (n=4). (H) Representative immunoblots for NLRP3 proteins in SCAP+/+ mice & SCAPD443N mice (n=3). (I) Representative images of immuno?uorescence staining of NLRP3 proteins in the aortas of SCAPD443N mice & SCAP+/+ mice (n=5). The data are presented as the means±SD of 3-5 independent experiments. *P<0.05, vs. the SCAP+/+ group. Statistical significance was calculated for the biological replicates by 2-tailed Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-31020241612244.jpg)
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free [NBP1-97601] - Sterol-resistant SCAP overexpression induces the activation of NLRP3 in?ammasomes in VSMCs. (A) Representative immunoblots for NLRP3, Procaspase-1, cleaved caspase-1, IL-1 beta , & IL-18 in SCAP-overexpressing VSMCs. (B) Expression of IL-1 beta & IL-18 in VSMC supernatants as measured by ELISA. (C) Immunoblotting of immunoprecipitation with anti-SCAP or anti-NLRP3 in SCAP-overexpressing VSMCs. (D) Representative images of immuno?uorescence staining of NLRP3 (green) or SCAP (green) with Golgi(red) in control & SCAP-overexpressing VSMCs. (E) Representative immunoblot for NLRP3, Pro-caspase-1, cleaved caspase-1, IL-1 beta , & IL-18 in SCAP-overexpressing VSMCs treated with lycorine for 4 h. (F) Expression of IL-1 beta & IL-18 in VSMC supernatants as measured by ELISA. (G) Representative images of the immuno?uorescence staining of NLRP3 (green) or SCAP (green) with Golgi (red) in SCAP VSMCs after treatment with lycorine for 4 h. The data are presented as the means±SD of 3 independent experiments. *P<0.05, vs the control group. Statistical significance was calculated for to the biological replicates by 2-tailed Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-31020241612193.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in RH-35 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0009.jpg "Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in RH-35 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Immunofluorescence analysis of paraffin-embedded human stomach cancer tissue labeling alpha-smooth muscle Actin (washed with PBS. And then probed with the primary antibody alpha smooth muscle Actin (red) at 1/1,000 dilution and ALDH2 (green) at 1/100 dilution overnight at 4 , washed with PBS. iFluor(TM) 488 conjugate-Goat anti-Mouse IgG and iFluor(TM) 647 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0012.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of Jurkat cells with alpha-Smooth Muscle Actin Antibody (SY02-64) at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Flow-Cytometry-NBP2-67440-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in A431 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in A549 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in HepG2 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of paraffin-embedded human gastric carcinoma tissue using alpha-Smooth Muscle Actin Antibody (SY02-64). Counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0005.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of paraffin-embedded human liver tissue using alpha-Smooth Muscle Actin Antibody (SY02-64). Counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0006.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of paraffin-embedded human tonsil tissue using alpha-Smooth Muscle Actin Antibody (SY02-64). Counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0007.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Immunofluorescence analysis of paraffin-embedded human Kidney tissue labeling alpha smooth muscle Actin (washed with PBS. And then probed with the primary antibody alpha smooth muscle Actin (red) at 1/1,000 dilution overnight at 4 , washed with PBS. iFluor(TM) 647 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0011.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of alpha smooth muscle Actin on different lysates with Rabbit anti-alpha smooth muscle Actin antibody at 1/1,000 dilution.Lane 1: Hela-si NT cell lysate (10 ug/Lane)Lane 2: Hela-si alpha smooth muscle Actin cell lysate (10 ug/Lane)Predicted band size: 42 kDaObserved band size: 42 kDaExposure time: 5 seconds;4-20% SDS-PAGE gel. was shown to specifically react with alpha smooth muscle Actin in Hela-si NT cells. Weakened band was observed when Hela-si alpha smooth muscle Actin sample was tested. Hela-si NT and Hela-si alpha smooth muscle Actin samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67440_rabbit-monoclonal-alpha-smooth-muscle-actin-antibody-sy02-64-western-blot-2372024113558..jpg)
![Western Blot: MKL2 Antibody [NBP1-46209] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MKL2 antibody NBP1-46209 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MKL2 was also immunoprecipitated by rabbit anti-MKL2 antibody NBP1-46208, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).](http://images.novusbio.com/fullsize/MKL2-Antibody-Western-Blot-NBP1-46209-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: MKL2 Antibody [NBP1-46209] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MKL2 antibody NBP1-46209 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MKL2 was also immunoprecipitated by rabbit anti-MKL2 antibody NBP1-46208, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: MKL2 Antibody [NBP1-46209] - Section of human prostate carcinoma and linitis plastica stomach cancer . Antibody: Affinity purified rabbit anti-MKL2 used at a dilution of 1:200 (1ug/ml) and 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MKL2-Antibody-Immunohistochemistry-NBP1-46209-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat trigeminal at 1 : 500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-98844-img0018.jpg "Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat trigeminal at 1 : 500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained appearing in blue.")
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat brain at 1:500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-98844-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat olfactory bulb at 1:500 dilution using Rabbit antibody to TRPC1: whole serum, DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-98844-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat brain at 1:500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-98844-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat brain at 1:500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-98844-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat trigeminal at 1:500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-98844-img0017.jpg)
![Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Western-Blot-NBP2-80765-img0001.jpg "Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80765-img0002.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - HIF-1 alpha (clone 2443C) was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) at 1.0 ug/mL for 1 hour at room temperature followed by incubation with the](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80765-img0003.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Simple-Western-NBP2-80765-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Knockout-Validated-NBP2-80765-img0004.jpg)
![Immunohistochemistry-Paraffin: BPGM Antibody [NBP1-86064] - Analysis in human placenta and liver tissues using NBP1-86064 antibody. Corresponding BPGM RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BPGM-Antibody-Immunohistochemistry-Paraffin-NBP1-86064-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BPGM Antibody [NBP1-86064] - Analysis in human placenta and liver tissues using NBP1-86064 antibody. Corresponding BPGM RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BPGM Antibody [NBP1-86064] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/BPGM-Antibody-Immunohistochemistry-Paraffin-NBP1-86064-img0017.jpg)
![Immunohistochemistry-Paraffin: BPGM Antibody [NBP1-86064] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/BPGM-Antibody-Immunohistochemistry-Paraffin-NBP1-86064-img0014.jpg)
![Immunohistochemistry-Paraffin: BPGM Antibody [NBP1-86064] - Staining of human urinary bladder shows weak to moderate cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/BPGM-Antibody-Immunohistochemistry-Paraffin-NBP1-86064-img0015.jpg)
![Immunohistochemistry-Paraffin: BPGM Antibody [NBP1-86064] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/BPGM-Antibody-Immunohistochemistry-Paraffin-NBP1-86064-img0016.jpg)
![Western Blot: APOA1BP Antibody [NBP2-30626] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-NAXE antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/APOA1BP-Antibody-Western-Blot-NBP2-30626-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: APOA1BP Antibody [NBP2-30626] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-NAXE antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Immunohistochemistry-Paraffin: APOA1BP Antibody [NBP2-30626] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-NAXE antibody NBP2-30626 (A) shows similar protein distribution across tissues to independent antibody NBP2-30943 (B).](http://images.novusbio.com/fullsize/APOA1BP-Antibody-Immunohistochemistry-Paraffin-NBP2-30626-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: APOA1BP Antibody [NBP2-30626] - Staining of human cell line A549 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/APOA1BP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30626-img0004.jpg)
![Immunohistochemistry-Paraffin: APOA1BP Antibody [NBP2-30626] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/APOA1BP-Antibody-Immunohistochemistry-Paraffin-NBP2-30626-img0011.jpg)
![Immunohistochemistry-Paraffin: APOA1BP Antibody [NBP2-30626] - Staining of human kidney.](http://images.novusbio.com/fullsize/APOA1BP-Antibody-Immunohistochemistry-Paraffin-NBP2-30626-img0008.jpg)
![Immunohistochemistry-Paraffin: APOA1BP Antibody [NBP2-30626] - Staining of human testis.](http://images.novusbio.com/fullsize/APOA1BP-Antibody-Immunohistochemistry-Paraffin-NBP2-30626-img0009.jpg)
![Immunohistochemistry-Paraffin: APOA1BP Antibody [NBP2-30626] - Staining of human lymph node.](http://images.novusbio.com/fullsize/APOA1BP-Antibody-Immunohistochemistry-Paraffin-NBP2-30626-img0010.jpg)
![Western Blot: Apolipoprotein L1 Antibody [NBP1-89033] - Analysis in human cell lines A-431 and MCF-7 using anti-APOL1 antibody. Corresponding APOL1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Apolipoprotein-L1-Antibody-Western-Blot-NBP1-89033-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Apolipoprotein L1 Antibody [NBP1-89033] - Analysis in human cell lines A-431 and MCF-7 using anti-APOL1 antibody. Corresponding APOL1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.")
![Simple Western: Apolipoprotein L1 Antibody [NBP1-89033] - Simple Western lane view shows a specific band for APOL1 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Apolipoprotein-L1-Antibody-Simple-Western-NBP1-89033-img0005.jpg)
![Simple Western: Apolipoprotein L1 Antibody [NBP1-89033] - Electropherogram image(s) of corresponding Simple Western lane view. Apolipoprotein L1 antibody was used at 1:30 dilution on RT-4 and U-251MG sp lysates(s).](http://images.novusbio.com/fullsize/Apolipoprotein-L1-Antibody-Simple-Western-NBP1-89033-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Immunofluorescence Analysis of HeLa cells labeled with alpha-Smooth Muscle Actin Antibody (ACTA/791) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunocytochemistry-Immunofluorescence-NBP2-44463-img0008.jpg "Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Immunofluorescence Analysis of HeLa cells labeled with alpha-Smooth Muscle Actin Antibody (ACTA/791) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Rat Lung stained with alpha-Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44463-img0004.jpg)
![Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (ACTA2/791) NBP2-47698PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Flow-Intracellular-NBP2-44463-img0007.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Human Angiosarcoma stained with alpha-Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44463-img0001.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Human Colon Carcinoma stained with alpha-Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44463-img0002.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Human Leiomyosarcoma stained with alpha-Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44463-img0003.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - An intracellular stain was performed on HepG2 cells with alpha-Smooth Muscle Actin Antibody (ACTA2/791) NBP2-47698PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Flow-Cytometry-NBP2-44463-img0006.jpg)
![Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - A. 30 ug PC-12 whole cell lysate/extract 7.5% SDS-PAGE tPA antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/t-Plasminogen-Activator-tPA-Antibody-Western-Blot-NBP2-20648-img0019.jpg "Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - A. 30 ug PC-12 whole cell lysate/extract 7.5% SDS-PAGE tPA antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Colon carcinoma. tPA antibody dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/t-Plasminogen-Activator-tPA-Antibody-Immunohistochemistry-Paraffin-NBP2-20648-img0021.jpg)
![Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Sample (30 ug of whole cell lysate) A: 293T 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/t-Plasminogen-Activator-tPA-Antibody-Western-Blot-NBP2-20648-img0017.jpg)
![Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Human hepatoma. tPA antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/t-Plasminogen-Activator-tPA-Antibody-Immunohistochemistry-Paraffin-NBP2-20648-img0020.jpg)
![Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with t-Plasminogen Activator/tPA antibody (NBP2-20648) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20648_rabbit-polyclonal-t-plasminogen-activator-tpa-antibody-91020242011476.jpg)
![Western Blot: CD63 Antibody (7G4.2E8) [NBP2-36567] - Western blot analysis of mouse CD63 on recombinant mouse CD63 and mouse kidney lysate.](http://images.novusbio.com/fullsize/CD63-Antibody-7G4-2E8-Western-Blot-NBP2-36567-img0002.jpg "Western Blot: CD63 Antibody (7G4.2E8) [NBP2-36567] - Western blot analysis of mouse CD63 on recombinant mouse CD63 and mouse kidney lysate.")
![Flow Cytometry: CD63 Antibody (7G4.2E8) [NBP2-36567] - Intracellular staining of mouse CD63 in Flow cytometry using 2.0 ug of antibody per 1 million cells. Isotype control was rat IgG2a lambda.](http://images.novusbio.com/fullsize/CD63-Antibody-7G4-2E8-Flow-Cytometry-NBP2-36567-img0003.jpg)
![Western Blot: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ACAA2-Antibody-OTI1C10-Western-Blot-NBP2-46477-img0009.jpg "Western Blot: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ACAA2-Antibody-OTI1C10-Immunohistochemistry-NBP2-46477-img0007.jpg)
![Western Blot: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAA2.](http://images.novusbio.com/fullsize/ACAA2-Antibody-OTI1C10-Western-Blot-NBP2-46477-img0008.jpg)
![Western Blot: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of extracts (10ug) from 7 Human tissues ( 1: Uterus; 2: Breast; 3: Brain; 4: Liver; 5: Ovary; 6: Thyroid gland; 7: colon)](http://images.novusbio.com/fullsize/ACAA2-Antibody-OTI1C10-Western-Blot-NBP2-46477-img0010.jpg)
![Immunohistochemistry: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ACAA2-Antibody-OTI1C10-Immunohistochemistry-NBP2-46477-img0003.jpg)
![Immunohistochemistry: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ACAA2-Antibody-OTI1C10-Immunohistochemistry-NBP2-46477-img0004.jpg)
![Immunohistochemistry: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ACAA2-Antibody-OTI1C10-Immunohistochemistry-NBP2-46477-img0005.jpg)
![Immunohistochemistry: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ACAA2-Antibody-OTI1C10-Immunohistochemistry-NBP2-46477-img0006.jpg)
![Immunofluorescence: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ACAA2.](http://images.novusbio.com/fullsize/ACAA2-Antibody-OTI1C10-Immunofluorescence-NBP2-46477-img0001.jpg)
![Immunofluorescence: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/ACAA2-Antibody-OTI1C10-Immunofluorescence-NBP2-46477-img0002.jpg)