| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50449-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43933 | |||
| BSA Free | NBP2-61617-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75786 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48532-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86064-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-30626-25ul | |||
![ELISA: Human Ceruloplasmin ELISA Kit (Colorimetric) [NBP2-60616] - Representative standard curve.](http://images.novusbio.com/fullsize/Human-Ceruloplasmin-ELISA-Kit-Colorimetric-ELISA-NBP2-60616-img0001.jpg "ELISA: Human Ceruloplasmin ELISA Kit (Colorimetric) [NBP2-60616] - Representative standard curve.")
![Western Blot: ATG5 Antibody (8L7X6) [NBP3-15854] - Western blot analysis of extracts of various cell lines, using ATG5 antibody (NBP3-15854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/ATG5-Antibody-8L7X6-Western-Blot-NBP3-15854-img0003.jpg "Western Blot: ATG5 Antibody (8L7X6) [NBP3-15854] - Western blot analysis of extracts of various cell lines, using ATG5 antibody (NBP3-15854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Western Blot: ATG5 Antibody (8L7X6) [NBP3-15854] - Western blot analysis of extracts from normal (control) and ATG5 knockout (KO) 293T cells, using ATG5 antibody (NBP3-15854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/ATG5-Antibody-8L7X6-Knockout-Validated-NBP3-15854-img0001.jpg)
![Immunoprecipitation: ATG5 Antibody (8L7X6) [NBP3-15854] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug ATG5 antibody (NBP3-15854). Western blot was performed from the immunoprecipitate using ATG5 (NBP3-15854) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/ATG5-Antibody-8L7X6-Immunoprecipitation-NBP3-15854-img0004.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-5220251726014.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Mouse colon tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517251919.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-5220251720294.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-5220251726015.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Rat colon tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517273524.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Human colon cancer tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517213515.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-522025171896.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Human colon tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517284996.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded rat colon tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517152727.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517172826.jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody (ARQ4974) [NBP3-15338] -Analysis of paraffin-embedded Human stomach using Lysozyme (LYZ) Rabbit mAb at a dilution of 1:400 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15338_rabbit-lysozyme-mab-arq4974-immunohistochemistry-paraffin-111020238055..jpg "Immunohistochemistry-Paraffin: Lysozyme Antibody (ARQ4974) [NBP3-15338] -Analysis of paraffin-embedded Human stomach using Lysozyme (LYZ) Rabbit mAb at a dilution of 1:400 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: Lysozyme Antibody (ARQ4974) [NBP3-15338] -Analysis of Lysozyme (LYZ) in paraffin-embedded mouse kidney tissue using Lysozyme (LYZ) Rabbit mAb at a dilution of 1:900 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15338_rabbit-lysozyme-mab-arq4974-immunohistochemistry-paraffin-11102023829..jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody (ARQ4974) [NBP3-15338] -Analysis of Lysozyme (LYZ) in paraffin-embedded human spleen tissue using Lysozyme (LYZ) Rabbit mAb at a dilution of 1:900 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15338_rabbit-lysozyme-mab-arq4974-immunohistochemistry-paraffin-111020238319..jpg)
![Western Blot: Lysozyme Antibody (ARQ4974) [NBP3-15338] - analysis of various lysates, using Lysozyme(LYZ) antibody at 1:20000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-15338_rabbit-lysozyme-mab-arq4974-western-blot-111020238449..jpg)
![Immunohistochemistry: Lysozyme Antibody (2Y5E4) [Lysozyme] - Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Lysozyme Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15338_rabbit-lysozyme-mab-2y5e4-52202517251934.jpg)
![Immunocytochemistry/ Immunofluorescence: Lysozyme Antibody (2Y5E4) [Lysozyme] - Confocal imaging of paraffin-embedded Mouse small intestine tissue using Lysozyme Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15338_rabbit-lysozyme-mab-2y5e4-52202517284995.jpg)
![Immunocytochemistry/ Immunofluorescence: Lysozyme Antibody (2Y5E4) [Lysozyme] - Confocal imaging of paraffin-embedded Mouse kidney tissue using Lysozyme Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15338_rabbit-lysozyme-mab-2y5e4-5220251719292.jpg)
![Immunocytochemistry/ Immunofluorescence: Lysozyme Antibody (2Y5E4) [Lysozyme] - Confocal imaging of RAW 264.7 cells using Lysozyme Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15338_rabbit-lysozyme-mab-2y5e4-52202517152741.jpg)
![Immunohistochemistry: Lysozyme Antibody (2Y5E4) [Lysozyme] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Lysozyme Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15338_rabbit-lysozyme-mab-2y5e4-52202517184591.jpg)
![Immunocytochemistry/ Immunofluorescence: Lysozyme Antibody (2Y5E4) [Lysozyme] - Confocal imaging of paraffin-embedded Mouse lung tissue using Lysozyme Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15338_rabbit-lysozyme-mab-2y5e4-52202517251993.jpg)
![Immunohistochemistry: Lysozyme Antibody (2Y5E4) [Lysozyme] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Lysozyme Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15338_rabbit-lysozyme-mab-2y5e4-52202517223971.jpg)
![Immunocytochemistry/ Immunofluorescence: Lysozyme Antibody (2Y5E4) [Lysozyme] - Immunofluorescence analysis of paraffin-embedded Human stomach tissue using Lysozyme Rabbit mAb at a dilution of 1:400 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15338_rabbit-lysozyme-mab-2y5e4-5220251719291.jpg)
![Immunohistochemistry: Lysozyme Antibody (2Y5E4) [Lysozyme] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Lysozyme Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15338_rabbit-lysozyme-mab-2y5e4-52202517273546.jpg)
![Immunoprecipitation: Integrin alpha V beta 6 Antibody (AvB6 53a.2) [NBP2-50449] - avb6 was immunoprecipitated with rat mAbs and western blotted for B6 with C19 antibody. Film was then exposed for different time exposures. The control lane was DX3puroB6 lysate. 59A, 10B2, 620W and 12D shows a very good result in revealing the double band for β6 by 5 seconds, and 53a2 shows only the upper band of B6. By 1 minute, most rat mAbs reveal a B6 immunoprecipitate. Data are from one of three separate experiments.](http://images.novusbio.com/fullsize/Integrin-alpha-V-+-beta-6-Antibody-AvB6-53a-2-Western-Blot-NBP2-50449-img0002.jpg "Immunoprecipitation: Integrin alpha V beta 6 Antibody (AvB6 53a.2) [NBP2-50449] - avb6 was immunoprecipitated with rat mAbs and western blotted for B6 with C19 antibody. Film was then exposed for different time exposures. The control lane was DX3puroB6 lysate. 59A, 10B2, 620W and 12D shows a very good result in revealing the double band for β6 by 5 seconds, and 53a2 shows only the upper band of B6. By 1 minute, most rat mAbs reveal a B6 immunoprecipitate. Data are from one of three separate experiments.")
![Western Blot: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Western blot analysis of Neuropilin-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat A](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-ST05-30-Western-Blot-NBP2-67539-img0009.jpg "Western Blot: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Western blot analysis of Neuropilin-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat A")
![Immunocytochemistry/Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Staining Neuropilin-1 in MCF-7 cells (green) using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-ST05-30-Immunocytochemistry-Immunofluorescence-NBP2-67539-img0004.jpg)
![Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of paraffin-embedded mouse kidney tissue using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-ST05-30-Immunohistochemistry-Paraffin-NBP2-67539-img0007.jpg)
![Flow Cytometry: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of Hela cells with Neuropilin-1 antibody (ST05-30) [NBP2-67539] at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-ST05-30-Flow-Cytometry-NBP2-67539-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Staining Neuropilin-1 in HUVEC cells (green) using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-ST05-30-Immunocytochemistry-Immunofluorescence-NBP2-67539-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Staining Neuropilin-1 in SHG-44 cells (green) using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-ST05-30-Immunocytochemistry-Immunofluorescence-NBP2-67539-img0003.jpg)
![Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of paraffin-embedded human kidney tissue using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-ST05-30-Immunohistochemistry-Paraffin-NBP2-67539-img0005.jpg)
![Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of paraffin-embedded human liver tissue using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-ST05-30-Immunohistochemistry-Paraffin-NBP2-67539-img0006.jpg)
![Western Blot: CYP1B1 Antibody [NBP2-50551] - Analysis of human CYP1B1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide.](http://images.novusbio.com/fullsize/CYP1B1-Antibody-Western-Blot-NBP2-50551-img0001.jpg "Western Blot: CYP1B1 Antibody [NBP2-50551] - Analysis of human CYP1B1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide.")
![Immunohistochemistry: CYP1B1 Antibody [NBP2-50551] - Analysis of human testis using this antibody.](http://images.novusbio.com/fullsize/CYP1B1-Antibody-Immunohistochemistry-NBP2-50551-img0002.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0021.jpg "Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.")
![Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody [NBP1-78007]](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78007-img0015.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Human Heart (formalin-fixed, paraffin-embedded) stained with beta Amyloid antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0024.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Mouse Brain (Lane 1) and Mouse Spinal Chord (Lane 2) were run on a 4-20% gradient gel, Blocked in 1% BSA-TBS-T 30 min RT and probed with Rb-a-Beta Amyloid 1:1000 in 1% BSA-TBS-T o/n 4C. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0010.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Lane 1: HEK293 WCL. Lane 2: Mouse Brain WCL. Lane 3: A-172 WCL. Load: 10.0ug per lane. Primary antibody: Beta Amyloid Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Conjugated Goat Anti Rabbit IgG at 1:40,000 for 30 min at RT. Block for 30 min at RT. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0018.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate.Lane 3: MCF-7 Lysate.Lane 4: Jurkat Lysate.Lane 5: A431 Lysate.Lane 6: LNCaP Lysate.Lane 7: A-172 Lysate.Lane 8: NIH/3T3 Lysate. Load: 35 ug per lane.Primary antibody: beta Amyloid antibody at 1:5,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT.Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted MW: ~40-50 kDa. Observed size: ~48 kDa for beta Amyloid.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0019.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Lane 5: Daudi Whole Cell Lysate. Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0020.jpg)
![Immunohistochemistry-Paraffin: beta Amyloid Antibody [NBP1-78007] - Human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-Paraffin-NBP1-78007-img0008.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistical detection of beta Amyloid using beta Amyloid antibody on TG APP23 mouse brain cortex frozen sections. beta Amyloid antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween/azide. Fluorescent labelled anti rabbit IgG was then added. Carl Hobbs, King`s College London, United Kingdom](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0022.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistry with beta Amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit beta Amyloid antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0023.jpg)
![ELISA: beta Amyloid Antibody [NBP1-78007] - ELISA results of purified beta Amyloid antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-ELISA-NBP1-78007-img0025.jpg)
![Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP2-61617] - Analysis in human liver and skeletal muscle tissues using NBP2-61617 antibody. Corresponding HMGCR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HMG-CoA-Reductase-HMGCR-Antibody-CL0260-Immunohistochemistry-Paraffin-NBP2-61617-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP2-61617] - Analysis in human liver and skeletal muscle tissues using NBP2-61617 antibody. Corresponding HMGCR RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP2-61617] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HMGCR antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/HMG-CoA-Reductase-HMGCR-Antibody-CL0260-Western-Blot-NBP2-61617-img0001.jpg)
![Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP2-61617] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/HMG-CoA-Reductase-HMGCR-Antibody-CL0260-Immunohistochemistry-Paraffin-NBP2-61617-img0015.jpg)
![Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP2-61617] - Staining of human breast cancer shows moderate to strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/HMG-CoA-Reductase-HMGCR-Antibody-CL0260-Immunohistochemistry-Paraffin-NBP2-61617-img0011.jpg)
![Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP2-61617] - Staining of human liver shows moderate cytoplasmic postivity in hepatocytes.](http://images.novusbio.com/fullsize/HMG-CoA-Reductase-HMGCR-Antibody-CL0260-Immunohistochemistry-Paraffin-NBP2-61617-img0012.jpg)
![Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP2-61617] - Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/HMG-CoA-Reductase-HMGCR-Antibody-CL0260-Immunohistochemistry-Paraffin-NBP2-61617-img0013.jpg)
![Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP2-61617] - Staining of human liver cancer shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/HMG-CoA-Reductase-HMGCR-Antibody-CL0260-Immunohistochemistry-Paraffin-NBP2-61617-img0014.jpg)
![Western Blot: Glut1 Antibody (GLUT1/2475) [NBP2-75784] - Western Blot Analysis of Human A431 cell lysate using Glut1 antibody.](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2475-Western-Blot-NBP2-75784-img0006.jpg "Western Blot: Glut1 Antibody (GLUT1/2475) [NBP2-75784] - Western Blot Analysis of Human A431 cell lysate using Glut1 antibody.")
![Protein Array: Glut1 Antibody (GLUT1/2475) [NBP2-75784] - Protein Array containing more than 19,000 full-length human proteins using Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2475-Protein-Array-NBP2-75784-img0005.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody (GLUT1/2475) [NBP2-75784] - Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human tongue stained with GLUT-1 Mouse Monoclonal Antibody (GLUT1/2475).](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2475-Immunohistochemistry-Paraffin-NBP2-75784-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Glut1 Antibody (GLUT1/2475) [NBP2-75784] - Immunofluorescence staining of K562 cells using Glut1Mouse Monoclonal Antibody (GLUT1/2475) followed by goat anti-Mouse IgG-CF488 (green). Nuclei are stained with Reddot.](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2475-Immunocytochemistry-Immunofluorescence-NBP2-75784-img0007.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody (GLUT1/2475) [NBP2-75784] - Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human bladder carcinoma stained with GLUT-1 Mouse Monoclonal Antibody (GLUT1/2475).](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2475-Immunohistochemistry-Paraffin-NBP2-75784-img0002.jpg)
![Flow Cytometry: Glut1 Antibody (GLUT1/2475) [NBP2-75784] - Flow Cytometric analysis of K562 cells using Glut1 antibody followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2475-Flow-Cytometry-NBP2-75784-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody [NBP2-48532] - Staining of human cell line Hep G2 shows localization to lipid droplets. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48532-img0006.jpg "Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody [NBP2-48532] - Staining of human cell line Hep G2 shows localization to lipid droplets. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Western Blot-Perilipin-2/ADFP Antibody [NBP2-48532] -Analysis in human cell lines RT-4 and U-251MG using Anti-PLIN2 antibody. Corresponding PLIN2 RNA-seq data are presented for the same cell lines.](http://images.novusbio.com/fullsize/antibody/nbp2-48532_rabbit-polyclonal-perilipin-2-adfp-antibody-western-blot-285202412135..jpg)
![Western Blot: APOA1BP Antibody [NBP2-30626] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-NAXE antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/APOA1BP-Antibody-Western-Blot-NBP2-30626-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: APOA1BP Antibody [NBP2-30626] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-NAXE antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Immunohistochemistry-Paraffin: APOA1BP Antibody [NBP2-30626] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-NAXE antibody NBP2-30626 (A) shows similar protein distribution across tissues to independent antibody NBP2-30943 (B).](http://images.novusbio.com/fullsize/APOA1BP-Antibody-Immunohistochemistry-Paraffin-NBP2-30626-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: APOA1BP Antibody [NBP2-30626] - Staining of human cell line A549 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/APOA1BP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30626-img0004.jpg)
![Immunohistochemistry-Paraffin: APOA1BP Antibody [NBP2-30626] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/APOA1BP-Antibody-Immunohistochemistry-Paraffin-NBP2-30626-img0011.jpg)
![Immunohistochemistry-Paraffin: APOA1BP Antibody [NBP2-30626] - Staining of human kidney.](http://images.novusbio.com/fullsize/APOA1BP-Antibody-Immunohistochemistry-Paraffin-NBP2-30626-img0008.jpg)
![Immunohistochemistry-Paraffin: APOA1BP Antibody [NBP2-30626] - Staining of human testis.](http://images.novusbio.com/fullsize/APOA1BP-Antibody-Immunohistochemistry-Paraffin-NBP2-30626-img0009.jpg)
![Immunohistochemistry-Paraffin: APOA1BP Antibody [NBP2-30626] - Staining of human lymph node.](http://images.novusbio.com/fullsize/APOA1BP-Antibody-Immunohistochemistry-Paraffin-NBP2-30626-img0010.jpg)