| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24716SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56080SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-26297SS | |||
| Azide and BSA Free | NBP2-80867 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-68133SS | |||
| Azide and BSA Free | NBP2-80866 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60735SS | |||
| Azide and BSA Free | NBP2-80831 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54790SS | |||
| Azide and BSA Free | NBP2-80644 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22116SS | |||
| Azide and BSA Free | NBP2-80689 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05936SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-21678 | |||
| DyLight 488 | NBP1-77701 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47777-0.1mg | |||
| IHC-Prediluted | NBP2-48207 | |||
![Western Blot: MTSS1 Antibody [NBP2-24716] - Analysis of MTSS1 in human testis lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse and 4) rat testis lysate using NBP2-24716 at 2 ug/ml, 4 ug/ml and 2 ug/ml, respectively.](http://images.novusbio.com/fullsize/MTSS1-Antibody-Western-Blot-NBP2-24716-img0005.jpg "Western Blot: MTSS1 Antibody [NBP2-24716] - Analysis of MTSS1 in human testis lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse and 4) rat testis lysate using NBP2-24716 at 2 ug/ml, 4 ug/ml and 2 ug/ml, respectively.")
![Immunohistochemistry-Paraffin: MTSS1 Antibody [NBP2-24716] - Analysis of human colon using this antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/MTSS1-Antibody-Immunohistochemistry-Paraffin-NBP2-24716-img0004.jpg)
![Western Blot: Bad Antibody [NB100-56080] - Analysis of Bad in Daoy whole cell lysate using anti-Bad antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Bad-Antibody-Western-Blot-NB100-56080-img0002.jpg "Western Blot: Bad Antibody [NB100-56080] - Analysis of Bad in Daoy whole cell lysate using anti-Bad antibody. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Bad Antibody [NB100-56080] - Immunofluorescence microscopy of BAD using NB100-56080 at 1:2000. Du145 human prostate carcinoma cells were cultured without (A) or with (B) and (C) 1 uM of the triphosphatase inhibitor thapsigargin (THG) for 12 hr. A and B, staining with BAD antibody, followed by a FITC-conjugated secondary antibody. C, staining with a mitochondrial marker (antibody to mitochondrial Hsp60), followed by a rhodamine-conjugated secondary antibody. THG induces Ca2+ release from internal stores which can promote apoptosis. BAD staining was located diffusely throughout the cytoplasm of untreated cells (A), and localized to the mitochondria in treated cells (B).](http://images.novusbio.com/fullsize/Bad-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56080-img0003.jpg)
![Immunohistochemistry-Paraffin: Bad Antibody [NB100-56080] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human liver using 1:2000 conc. of Bad antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Bad was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Bad-Antibody-Immunohistochemistry-Paraffin-NB100-56080-img0004.jpg)
![Western Blot: Nbs1 Antibody (7E4A2) [NBP2-26297] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human Polyclonal Nbs1 Antibody [NBP2-26297] followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody [HAF018]. Specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Knockout-Validated-NBP2-26297-img0015.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Nbs1 Antibody (7E4A2) [NBP2-26297] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human Polyclonal Nbs1 Antibody [NBP2-26297] followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody [HAF018]. Specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis of Hela cells using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] (green). Blue: DRAQ5 fluorescent DNA dye [NBP2-81125]. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/NBS1-Antibody-7E4A2-Immunocytochemistry-Immunofluorescence-NBP2-26297-img0008.jpg)
![Immunohistochemistry: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis of paraffin-embedded rectum cancer tissues using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] with DAB staining.](http://images.novusbio.com/fullsize/NBS1-Antibody-7E4A2-Immunohistochemistry-NBP2-26297-img0011.jpg)
![Flow Cytometry: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis of Nbs1 in Hela cells using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] (green) and negative control (red).](http://images.novusbio.com/fullsize/NBS1-Antibody-7E4A2-Flow-Cytometry-NBP2-26297-img0009.jpg)
![Immunohistochemistry-Paraffin: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis of Nbs1 in paraffin-embedded cervical cancer tissues using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] with DAB staining.](http://images.novusbio.com/fullsize/NBS1-Antibody-7E4A2-Immunohistochemistry-NBP2-26297-img0010.jpg)
![ELISA: Nbs1 Antibody (7E4A2) [NBP2-26297] - ELISA analysis using Nbs1 Antibody (7E4A2) [NBP2-26297].](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-ELISA-NBP2-26297-img0014.jpg)
![Simple Western: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis using Nbs1 Antibody (7E4A2) [NBP2-26297]. Molecular Weight observed at: 115 kDa.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Simple-Western-NBP2-26297-img0017.jpg)
![Western Blot: Napsin-A Antibody (TMU-Ad02) [NB110-68133] - Analysis of NAPSA expression in human lung using NB110-68133.](http://images.novusbio.com/fullsize/Napsin-A-Antibody-TMU-Ad02-Western-Blot-NB110-68133-img0009.jpg "Western Blot: Napsin-A Antibody (TMU-Ad02) [NB110-68133] - Analysis of NAPSA expression in human lung using NB110-68133.")
![Immunohistochemistry-Paraffin: Napsin-A Antibody (TMU-Ad02) [NB110-68133] - Analysis of NAPSA on human lung adenocarcinoma using NB110-68133.](http://images.novusbio.com/fullsize/Napsin-A-Antibody-TMU-Ad02-Immunohistochemistry-Paraffin-NB110-68133-img0008.jpg)
![Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Knockout-Validated-NBP2-60735-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Simple Western: LC3B Antibody (1251B) [NBP2-60735] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours, loaded at 0.2 mg/mL. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human/Mouse/Rat anti-LC3B Monoclonal Antibody (1251B) [Catalog # NBP2-60735]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Simple-Western-NBP2-60735-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- [Catalog # NBP2-60735] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunocytochemistry-Immunofluorescence-NBP2-60735-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Western-Blot-NBP2-60735-img0010.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody (1251B) [NBP2-60735] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251B) [Catalog # NBP2-60735] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB (3,3'Diaminobenzidine) reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This LC3 antibody generated a diffused staining in all cell types (except the endothelial cells of blood vessels) and the signal was strongest in a subset of neuronal cells. A few cells depicted LC3 puncta formation/dotted staining in apparently autophagic cells.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunohistochemistry-Paraffin-NBP2-60735-img0011.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were either (A) untreated or (B) treated with 50uM chloroquine for 24 hours. An intracellular stain was performed with anti-LC3B (1251B) antibody [Catalog # NBP2-60735] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Flow-Intracellular-NBP2-60735-img0009.jpg)
![Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CD36 Monoclonal antibody (catalog number NBP2-54790) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). A specific band was detected for CD36/SR-B3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Western-Blot-NBP2-54790-img0023.jpg "Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CD36 Monoclonal antibody (catalog number NBP2-54790) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). A specific band was detected for CD36/SR-B3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: CD36 Antibody (1283D) [NBP2-54790] - CD36 was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human CD36/SR-B3 Monoclonal Antibody for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM) HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocyte membranes.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Immunohistochemistry-Paraffin-NBP2-54790-img0002.jpg)
![Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Total protein from human skin and adipose tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-CD36 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Western-Blot-NBP2-54790-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD36 Antibody (1283D) [NBP2-54790] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-CD36 (1283D) at 5.0 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective..](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Immunocytochemistry-Immunofluorescence-NBP2-54790-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - 3T3-L1 adipocytes were pretreated and treated with ML348 (10 uM) for 1 h, followed by BSA or oleate treatment for another 1 h. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD36-Antibody-1283D-BSA-Free-Western-Blot-NBP2-54790-img0024.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - LYN phosphorylates DHHC5 & is required for the endocytosis of FAs.a 3T3-L1 adipocytes pretreated with serum-free medium for 4 h & PP2 (15 μM) for 1 h, followed by treatment with BSA or oleate (100 μM) for 5 min. Cells harvested & immunoprecipitated with anti-DHHC5 antibody to detect phosphorylation of Tyr91. b 3T3-L1 adipocytes transduced with indicated shRNA set up & treated with oleate for 5 min. Cells harvested to detect Tyr91 phosphorylation as in (a). c On day 0, HEK293T cells set up at 2.5 × 105 cells per 6-cm dish. On day 2, cells transfected with 0.5 μg of WT, Y91E, or Y91F of DHHC5-Flag/pCDH-puro and/or 0.5 μg LYN/pCDNA3.3. On day 3, cells harvested & immunoprecipitated with anti-Flag M2 beads to detect phosphorylation of DHHC5 (pY). Control & LYN knockdown adipocytes pretreated & treated with BSA or oleate (100 μM) for 1 h, followed by immunostaining (d), surface biotinylation (e), & Acyl-RAC (f) assays. Control & CD36 knockdown 3T3-L1 adipocytes pretreated & treated with oleate (100 μM) for indicated time (g) or 5 min (h). Cells harvested & subjected to immunoprecipitation of LYN to detect phosphorylation of LYN (Y396). i, j WT & Cd36−/− SVFs set up & subjected to FA uptake as in Fig. 3h, i, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 20 cells. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. k WT & Cd36−/− adipocytes set up & subjected to 3H-oleate uptake as in Fig. 3j, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 3samples. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. These experiments repeated at least twice. Source data provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-3102024161365.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - LYN phosphorylates DHHC5 & is required for the endocytosis of FAs.a 3T3-L1 adipocytes pretreated with serum-free medium for 4 h & PP2 (15 μM) for 1 h, followed by treatment with BSA or oleate (100 μM) for 5 min. Cells harvested & immunoprecipitated with anti-DHHC5 antibody to detect phosphorylation of Tyr91. b 3T3-L1 adipocytes transduced with indicated shRNA set up & treated with oleate for 5 min. Cells harvested to detect Tyr91 phosphorylation as in (a). c On day 0, HEK293T cells set up at 2.5 × 105 cells per 6-cm dish. On day 2, cells transfected with 0.5 μg of WT, Y91E, or Y91F of DHHC5-Flag/pCDH-puro and/or 0.5 μg LYN/pCDNA3.3. On day 3, cells harvested & immunoprecipitated with anti-Flag M2 beads to detect phosphorylation of DHHC5 (pY). Control & LYN knockdown adipocytes pretreated & treated with BSA or oleate (100 μM) for 1 h, followed by immunostaining (d), surface biotinylation (e), & Acyl-RAC (f) assays. Control & CD36 knockdown 3T3-L1 adipocytes pretreated & treated with oleate (100 μM) for indicated time (g) or 5 min (h). Cells harvested & subjected to immunoprecipitation of LYN to detect phosphorylation of LYN (Y396). i, j WT & Cd36−/− SVFs set up & subjected to FA uptake as in Fig. 3h, i, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 20 cells. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. k WT & Cd36−/− adipocytes set up & subjected to 3H-oleate uptake as in Fig. 3j, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 3samples. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. These experiments repeated at least twice. Source data provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114783.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416121990.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-31020241612242.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114740.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114741.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - CD36-mediated caveolar endocytosis transports FAs into cells.a–c 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated PacFA (50 μM) for 20 min, followed by UV crosslinking on ice for 30 min. a, b Cells were subjected to click chemistry using an N3-Alexa Fluro 488, & immunostained with anti-CD36 antibody. Colocalization of PacFA & CD36 was quantified from 24 cells over three independent experiments & plotted in (b). The value represents mean ± SEM. c Cells were lysed & subjected to click chemistry assay using N3-biotin. PacFA-labeled proteins were captured with streptavidin beads & subjected to western blot using anti-CD36 & anti-FABP4 antibodies. d WT, Cav1−/−, Cd36−/−, & Cav1−/−;Cd36−/− SVFs were isolated & differentiated into adipocytes & treated with 100 μM 3H-oleate (specific activity, 2268 dpm/nmol) for 1 h. Lipid fractions were extracted from the cells & subjected to scintillation counting. The radioactive counting was normalized to protein content. Each value represents mean ± SEM obtained from three samples. Two-sided Student’s t test was performed between WT & each of the knockout cells. These experiments were repeated twice. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416113520.jpg)
![Western Blot: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Western blot analysis using Cytokeratin 19 mouse mAb against T47D (1), MCF-7 (2), HepG2 (3) and SW620 (4) cell lysate.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-Western-Blot-NBP2-22116-img0003.jpg "Western Blot: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Western blot analysis using Cytokeratin 19 mouse mAb against T47D (1), MCF-7 (2), HepG2 (3) and SW620 (4) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunofluorescence analysis of HepG2 cells using Cytokeratin 19 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-Immunocytochemistry-Immunofluorescence-NBP2-22116-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using Cytokeratin 19 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-Immunohistochemistry-Paraffin-NBP2-22116-img0007.jpg)
![Western Blot: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Western blot analysis using Cytokeratin 19 mAb against human Cytokeratin 19 (AA: 115-269) recombinant protein. (Expected MW is 43.1 kDa)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-Western-Blot-NBP2-22116-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using Cytokeratin 19 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-Immunohistochemistry-Paraffin-NBP2-22116-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using Cytokeratin 19 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-Immunohistochemistry-Paraffin-NBP2-22116-img0006.jpg)
![ELISA: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-ELISA-NBP2-22116-img0001.jpg)
![Western Blot: p73 Antibody [NB100-420] - Whole cell lysate (50 ug) from HEK293T, HeLa, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p73 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/p73-Antibody-Western-Blot-NB100-420-img0003.jpg "Western Blot: p73 Antibody [NB100-420] - Whole cell lysate (50 ug) from HEK293T, HeLa, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p73 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Western Blot: p73 Antibody [NB100-420] - Detection of Human p73 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-p73 antibody NB100-420 used for WB at 1 ug/ml (A and B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/p73-Antibody-Western-Blot-NB100-420-img0002.jpg)
![Western Blot: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Human stomach lysate.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody---BSA-Free-Western-Blot-NBP1-05936-img0011.jpg "Western Blot: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Human stomach lysate.")
![Immunocytochemistry/Immunofluorescence: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - AGR2 antibody was tested in MCF7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05936-img0012.jpg)
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Representative immunohistological analysis of CD163 (scavenger receptor present in macrophages), AGR2, and TMED2 in non-inflamed colonic biopsies from healthy controls and patients with active or quiescent CD. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/10.15252/emmm.201810120) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody---BSA-Free-Immunohistochemistry-NBP1-05936-img0014.jpg)
![Western Blot: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Western blot analysis (left panel) and quantification (right panel) of AGR2 intracellular expression upon TMED2 overexpression. Data are representative of four independent experiments. Tubulin (TUB) was used as a loading control. The graph represents average signal normalized on reporter protein expression +/- SD. The Mann-Whitney statistical test was used. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31040128/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AG-2-AGR2 Antibody - BSA Free-Western Blot-NBP1-05936-img0015.jpg)
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Detection of AGR2 in mouse prostate.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody---BSA-Free-Immunohistochemistry-NBP1-05936-img0009.jpg)
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Detection of AGR2 in mouse kidney.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody---BSA-Free-Immunohistochemistry-NBP1-05936-img0010.jpg)
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - B. Analysis of AGR2 expression in the proximal colon of WT and TMED2 hypomorph mice. C.Semi-quantitative analysis of AGR2 expression in the proximal colon of WT (blank bars) and TMED2 hypomorph mice (black bars) (n = 6). The graph represents average IHC signal +/- SD. For statistical analyses, the Mann-Whitney test was used. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/10.15252/emmm.201810120) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody---BSA-Free-Immunohistochemistry-NBP1-05936-img0013.jpg)
![Western Blot: BRCA1 Antibody (8F7) [NBP1-41186] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BRCA1-Antibody-8F7-Western-Blot-NBP1-41186-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (8F7) [NBP1-41186] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: BRCA1 Antibody (8F7) [NBP1-41186] - Paraffin-embedded Breast ca, using BRCA1 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/BRCA1-Antibody-8F7-Immunohistochemistry-NBP1-41186-img0002.jpg)
![Western Blot: BRCA1 Antibody (8F7) [NBP1-41186] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382 was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp1-41186_mouse-monoclonal-brca1-antibody-8f7-western-blot-81202411325..jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A431 cells. Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (alpha-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT pS473 at the cell periphery.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0011.jpg "Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A431 cells. Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (alpha-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT pS473 at the cell periphery.")
![Flow Cytometry: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis using the DyLight 488 conjugate of AKT1 phospho Ser473 antibody. Image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Flow-Cytometry-NBP1-69923-img0021.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT1 [p Ser473] antibody (17F6.B11). A: Lane 1) PDGF stimulated NIH 3T3 cells [10ul]. Lane 2) NIH 3T3 cells [10ul]. Lane 3) Hela whole cell lysate [10ul] (weak signal). B: Lane 4) GST negative control protein [100ng]. Lane 5) GST negative control protein [25ng]. Lane 6) AKT 1 recombinant protein [100ng]. Lane 7) AKT 1 recombinant protein [25ng].Block: 5% BSA overnight at 4C.Primary antibody: Monoclonal anti-AKT antibody used at 1:1000 for overnight at 4C.Secondary antibody: HRP Conjugated goat anti-mouse 1:25K for 45 min at RT.Detection: TMB for 20 minutes, rinsed with deionized water, dried and scanned on conventional flatbed scanner.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0045.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - High resolution STED immunofluorescence nanoscopy of AKT11 [p Ser473] antibody (17F6.B11). Tissue: A431 cells. The merge images (A) and at high magnification (B) show phosphorylated AKT1 colocalized with the distal microtubules. Fixation: 4% paraformaldehyde for 5 min and after washes blocked with 10% NGS/0.2% Triton X-100 for 30 min.Antigen retrieval: serum deprivation for 12 h.Primary antibody: AKT1 pS473 antibody at 10 ug/mL and alpha-tubulin (cyan) at 1.4 ug/mL for 1 h at RT.Secondary antibody: Atto 647N anti-Mouse IgG (ATTO TEC GmbH), and DyLight(TM)488 anti-Rabbit IgG were used at 1.0 ug/mL for 1h at RT for indirect detection.Localization: AKT1 pS473 is in the cytoplasm and also organized at the periphery of the cell.Staining: AKT1 pS473 as red signal with bis-benzimide (blue) nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0040.jpg)
![Immunohistochemistry: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunohistochemistry of AKT11 [p Ser473] antibody (17F6.B11). Tissue: human prostate tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: AKT1 pS473 antibody at 20 ug/mL for 1 h at RT.Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT.Localization: AKT1 pS473 is nuclear and occasionally cytoplasmic.Staining: AKT1 pS473 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-NBP1-69923-img0046.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunoblotting analysis. Representative images of the immunoblot analysis for (A) Akt, (B) p53 (C) AMPK, and (D) LC3 at 24 h of incubation with or without 20 mM dG are shown. beta-actin was used as loading control. Densitometry analysis was performed and the Akt-P/Akt ratio was calculated. The results are the mean +/- SEM of three independent experiments. * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/19/7/2115) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0037.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT11 [p Ser473] antibody (17F6.B11). Lane 1: untreated NIH/3T3 cell lysates. Lane 2: detects phosphorylated AKT1 (indicated by arrowhead at ~56 kDa) on PDGF stimulated NIH/3T3 cell lysates. Load: 10 ug per lane.Primary antibody: AKT1 pS473 antibody at 1:10,000 in TBS with 0.05% Tween-20 with 1% BSA, for 1 h at 4 C.Secondary antibody: HRP conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 1 h at 4 C with FemtoMax(TM) enhanced chemiluminescent reagent.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0042.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT11 [p Ser473] antibody (17F6.B11). Lane 1: A431 cells. Lane 2: A431 cells stimulated for 15 min with EGF. Load: 35 ug per lane.Primary antibody: AKT1pS473 antibody at 1:400 for overnight at 4C.Secondary antibody: DyLight(TM)649 Conjugated Anti-AKT1 pS473 Monoclonal Antibody at 1:10,000 for 45 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4C.Predicted/Observed size: 56kDa. Other band(s): none.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0043.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT1 [p Ser473] antibody (17F6.B11). Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated AKT11, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated phosphorylated AKT11 (red band). Load: 10 ug per lane.Primary antibody: rabbit anti-AKT1 (pan) and mouse anti-AKT1 pS473 specific antibodies at 1:400 for overnight at 4C.Secondary antibody: DyLight(TM) 549 conjugated anti-rabbit IgG (green) and DyLight(TM) 649 conjugated anti-mouse IgG (red) secondary antibodies at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0044.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of DyLight 488 conjugate of NBP1-69923. This image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0035.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923]](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0038.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunofluorescence Microscopy of AKT11 [p Ser473] antibody (17F6.B11) using STED nanoscopy to evaluate AKT1 activation and migration. Tissue: A431 cells. Antigen retrieval: Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins.A massive increase in AKT1-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin.Staining: Panel A shows STED data (AKT1-pS473, red channel) collected simultaneously with confocal signal (a-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT1 is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT1pS473 at the cell periphery.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0039.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of Biotin conjugate of NBP1-69923. 20 ug/mL for 1 h at RT Secondary antibody: Streptavidin Conj. HRP 10 ug/ml Localization: nuclear and occasionally cytoplasmic Staining: antibody as precipitated red signal with a hematoxylin purple nuclear count](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-Paraffin-NBP1-69923-img0034.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of FFPE prostate tissue using AKT1 phospho Ser473 antibody (left panel). Negative control (right panel). Antigen retrieval with heat and pressure in citrate buffer pH 6.2. AKT1 phospho Ser473 antibody at 20 ug/mL. Secondary antibody Streptavidin-HRP at 10 ug/mL. Hematoxylin nuclear counterstain (purple). Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-Paraffin-NBP1-69923-img0036.jpg)
![Western Blot of Mouse Anti-AKTpS473 antibody. Lane 1: A431 cells. Lane 2: A431 cells stimulated for 15 min with EGF. Load: 35 ug per lane. Primary antibody: AKTpS473 antibody at 1:400 for overnight at 4C. Secondary antibody: DyLight(TM)649 Conjugated Anti-AKT1 [p Ser473] Antibody (17F6.B11) at 1:10,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4C. Predicted/Observed size: 56kDa. Other band(s): none.](http://images.novusbio.com/fullsize/nbp1-69923_mouse-monoclonal-akt1-p-ser473-antibody-17f6-b11-255202313136.jpg)
![Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MUC18/1130) [NBP2-44512] - Human Tonsil stained with MUC18 Monoclonal Antibody (MUC18/1130)](http://images.novusbio.com/fullsize/MCAM-CD146-Antibody-MUC18-1130-Immunohistochemistry-Paraffin-NBP2-44512-img0003.jpg "Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MUC18/1130) [NBP2-44512] - Human Tonsil stained with MUC18 Monoclonal Antibody (MUC18/1130)")
![Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MUC18/1130) [NBP2-44512] - Human Melanoma stained with MUC18 Monoclonal Antibody (MUC18/1130)](http://images.novusbio.com/fullsize/MCAM-CD146-Antibody-MUC18-1130-Immunohistochemistry-Paraffin-NBP2-44512-img0001.jpg)
![Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MUC18/1130) [NBP2-44512] - Human Melanoma stained with MUC18 Monoclonal Antibody (MUC18/1130)](http://images.novusbio.com/fullsize/MCAM-CD146-Antibody-MUC18-1130-Immunohistochemistry-Paraffin-NBP2-44512-img0002.jpg)
![Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MUC18/1130) [NBP2-44512] - CD146 was stained in both human brain and pancreatic tissues. CD146 expression was high in pancreatic tissue, yet low in brain tissue as shown by the brown coloration. Image from a verified customer review.](http://images.novusbio.com/fullsize/MCAM-CD146-Antibody-MUC18-1130-Immunohistochemistry-Paraffin-NBP2-44512-img0006.jpg)
![Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MUC18/1130) [NBP2-44512] - CD146 Staining of Normal Human Tissues. Image from a verified customer review.](http://images.novusbio.com/fullsize/MCAM-CD146-Antibody-MUC18-1130-Immunohistochemistry-Paraffin-NBP2-44512-img0007.jpg)