| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44370 | |||
| Azide and BSA Free | NBP2-34394-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47738-0.1mg | |||
| IHC-Prediluted | NBP2-48082 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2384SS | |||
![Western Blot: IKK beta Antibody [NBP1-32258] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody diluted at 1:500.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Western-Blot-NBP1-32258-img0008.jpg "Western Blot: IKK beta Antibody [NBP1-32258] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: IKK beta Antibody [NBP1-32258] - analysis of A431, using IKK beta antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32258-img0002.jpg)
![Immunohistochemistry-Paraffin: IKK beta Antibody [NBP1-32258] - Paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-32258-img0003.jpg)
![Western Blot: IKK beta Antibody [NBP1-32258] - IKK beta antibody detects IKK beta protein by western blot analysis. A. 30 ug 293T whole cell extract. B. 30 ug whole cell extract of DDDDK-human IKBKB-transfected 293T cells. 7.5 % SDS-PAGE. IKK beta antibody dilution: 1:5000](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Western-Blot-NBP1-32258-img0006.jpg)
![Western Blot: IKK beta Antibody [NBP1-32258] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Western-Blot-NBP1-32258-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (SPM115 + SPM116) [NBP2-34347] - FFPE human breast carcinoma stained with pan cytokeratin monoclonal antibody cocktial (SPM115+SPM116).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-SPM115-+-SPM116-Immunohistochemistry-Paraffin-NBP2-34347-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (SPM115 + SPM116) [NBP2-34347] - FFPE human breast carcinoma stained with pan cytokeratin monoclonal antibody cocktial (SPM115+SPM116).")
![Flow Cytometry: Cytokeratin, pan Antibody (SPM115 + SPM116) [NBP2-34347] - Flow Cytometric analysis of human Cytokeratin, pans on HeLa cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cytokeratin, pan Monoclonal Antibody (SPM115 + SPM116).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-SPM115-+-SPM116-Flow-Cytometry-NBP2-34347-img0005.jpg)
![Flow (Intracellular): Cytokeratin, pan Antibody (SPM115 + SPM116) [NBP2-34347] - An intracellular stain was performed on HeLa cells with Cytokeratin, pan Antibody (SPM115 + SPM116) NBP2-34394APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin. Image using the Allophycocyanin format of this antibody.](http://images.novusbio.com/fullsize/nbp2-34347_mouse-monoclonal-cytokeratin-pan-antibody-spm115-spm116-17720241003222.jpg)
![Western Blot: Cullin 5 Antibody [NBP1-22970] - Whole cell lysate (10 ug) from NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cul5 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Cullin-5-Antibody-Western-Blot-NBP1-22970-img0008.jpg "Western Blot: Cullin 5 Antibody [NBP1-22970] - Whole cell lysate (10 ug) from NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cul5 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: Cullin 5 Antibody [NBP1-22970] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Cul5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Cullin-5-Antibody-Immunohistochemistry-NBP1-22970-img0006.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Cullin 5 Antibody [NBP1-22970] - by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50mcg) cells. Antibodies: Affinity purified rabbit anti-Cul5 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. Cul5 was also immunoprecipitated by another rabbit anti-Cul5 antibody, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/Cullin-5-Antibody-Western-Blot-NBP1-22970-img0002.jpg)
![Western Blot: Cullin 5 Antibody [NBP1-22970] - Whole cell lysate (50 ug) from HEK293T, LNCaP, and Hep-G2 cellsprepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cul5 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Cullin-5-Antibody-Western-Blot-NBP1-22970-img0007.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.](http://images.novusbio.com/fullsize/RelA-NFkB p65 [p Ser529] Antibody-Western Blot-NBP1-77808-img0005.jpg "Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.")
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - pS529 shows phospho p65 staining in carcinoma cells. western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS529 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0003.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 (NBP1-77808) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the western blot using a chemi-luminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0004.jpg)
![Immunohistochemistry-Paraffin: c-Myc Antibody (9E10.3) [NBP2-45144] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (9E10.3).](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-3-Immunohistochemistry-Paraffin-NBP2-45144-img0001.jpg "Immunohistochemistry-Paraffin: c-Myc Antibody (9E10.3) [NBP2-45144] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (9E10.3).")
![Simple Western: c-Myc Antibody (9E10.3) [NBP2-45144] - Simple Western lane view shows a specific band for c-Myc in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-3-Simple-Western-NBP2-45144-img0002.jpg)
![Simple Western: c-Myc Antibody (9E10.3) [NBP2-45144] - Electropherogram image of the corresponding Simple Western lane. c-Myc antibody was used at 50 ug/ml dilution of HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-3-Simple-Western-NBP2-45144-img0005.jpg)
![Simple Western: c-Myc Antibody (9E10.3) [NBP2-45144] - Lane view shows a specific band for c-Myc in 0.2 mg/mL of HeLa lysate and antibody at 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 74 kDa. Image reported by internal validation.](http://images.novusbio.com/fullsize/nbp2-45144_mouse-monoclonal-c-myc-antibody-9e10-3-1772024959410.jpg)
![Western Blot: MCM3 Antibody [NB100-289] - Detection of Human MCM3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM3 antibody NB100-289 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NB100-289-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM3 Antibody [NB100-289] - Detection of Human MCM3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM3 antibody NB100-289 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: MCM3 Antibody [NB100-289] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-MCM3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM3-Antibody-Immunohistochemistry-NB100-289-img0010.jpg)
![Western Blot: MCM3 Antibody [NB100-289] - RIPA extract (50 ug) from HeLa cells. Antibody used at 0.2 ug/ml.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NB100-289-img0002.jpg)
![Western Blot: Rabbit Polyclonal MCM3 Antibody [NB100-289] - Whole cell lysates from MDA-MB-231 and SUM159 cells were loaded with 50 ug/lane. 10% SDS-PAGE. MCM3 Antibody (NB100-289) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-289_rabbit-polyclonal-mcm3-antibody-western-blot-2122024155814..jpg)
![Western Blot: PDK-1 Antibody [NB100-2384] - Detection of PDK-1 in human heart lysate using NB 100-2384 at 0.5 ug/ml.](http://images.novusbio.com/fullsize/PDK-1-Antibody-Western-Blot-NB100-2384-img0005.jpg "Western Blot: PDK-1 Antibody [NB100-2384] - Detection of PDK-1 in human heart lysate using NB 100-2384 at 0.5 ug/ml.")
![Immunohistochemistry-Paraffin: PKC beta Antibody [NBP2-19846] - Rat brain. PKC beta antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PKC-beta-Antibody-Immunohistochemistry-Paraffin-NBP2-19846-img0005.jpg "Immunohistochemistry-Paraffin: PKC beta Antibody [NBP2-19846] - Rat brain. PKC beta antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![<b>Biological Strategies Validation. </b>Western Blot: PKC beta Antibody [NBP2-19846] - Human neuroblastoma cells untreated or treated with various drugs. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/PKC-beta-Antibody-Western-Blot-NBP2-19846-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: PKC beta Antibody [NBP2-19846] - PKC beta antibody detects PKC beta protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PKC beta stained by PKC beta antibody (NBP2-19846) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-19846_rabbit-polyclonal-pkc-beta-antibody-91020242041255.jpg)
![Western Blot: PKC beta Antibody [NBP2-19846] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC beta antibody (NBP2-19846) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19846_rabbit-polyclonal-pkc-beta-antibody-910202420134042.jpg)
![Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha-Actinin 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Western-Blot-NBP1-32578-img0013.jpg "Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha-Actinin 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody [NBP1-32578] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-32578-img0007.jpg)
![Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - A:NIH-3T3.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Western-Blot-NBP1-32578-img0008.jpg)
![Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody diluted at a dilution of 1:1000.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Western-Blot-NBP1-32578-img0011.jpg)
![Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha-Actinin 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Western-Blot-NBP1-32578-img0012.jpg)
![Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Whole zebrafish extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha-Actinin 1 antibody [N2N3] (NBP1-32578) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp1-32578_rabbit-polyclonal-alpha-actinin-1-antibody-91020242063315.jpg)
![Immunohistochemistry: alpha-Actinin 1 Antibody [NBP1-32578] - alpha-Actinin 1 antibody [N2N3] detects alpha-Actinin 1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. alpha-Actinin 1 antibody [N2N3] (NBP1-32578) dilution: 1:50.](http://images.novusbio.com/fullsize/nbp1-32578_rabbit-polyclonal-alpha-actinin-1-antibody-91020242091449.jpg)
![Western Blot: BIRC6 Antibody [NB300-264] - Detection of Human and Mouse BIRC6 by Western Blot. Samples: Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIRC6 antibody NB300-264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Western-Blot-NB300-264-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: BIRC6 Antibody [NB300-264] - Detection of Human and Mouse BIRC6 by Western Blot. Samples: Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIRC6 antibody NB300-264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: BIRC6 Antibody [NB300-264] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BIRC6/Apollon used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB Coutnerstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-Paraffin-NB300-264-img0008.jpg)
![Immunoprecipitation: BIRC6 Antibody [NB300-264] - Detection of human BIRC6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BIRC6 antibody NB300-264 (lot 2) used for IP at 6 ug per reaction. BIRC6 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated BIRC6, NB300-264 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunoprecipitation-NB300-264-img0013.jpg)
![Western Blot: Aldolase A Antibody [NB600-915] - Analysis using Biotin conjugate of NB600-915. 300 ul aliquots of whole anti-aldolase antiserum used to precipitate varying amounts of purified aldolase & precipitates with controls; compared by SDS-PAGE & Western blot. 1. Purified aldolase 2. 300 ul antiserum with no antigen (negative control) 3. 300 ul antiserum with 100 ul aldolase (2.5 mg/ml) 4. 300 ul antiserum with 200 ul aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum & an equal volume of aldolase antigen in PBS incubated 24 hrs at 4C, centrifuged for 6 mins at 13K RPM, washed once with PBS, centrifuged & dissolved in 60 ul 0.1 N NaOH. 90 ul of PBS was added, sample was divided in 2 portions, & an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer added. The reduced samples were boiled for five mins, & all samples were run at 140 V for 45 mins on a 4-20% tris/glycine gradient gel. Gel was stained, destained & imaged.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Western-Blot-NB600-915-img0010.jpg "Western Blot: Aldolase A Antibody [NB600-915] - Analysis using Biotin conjugate of NB600-915. 300 ul aliquots of whole anti-aldolase antiserum used to precipitate varying amounts of purified aldolase & precipitates with controls; compared by SDS-PAGE & Western blot. 1. Purified aldolase 2. 300 ul antiserum with no antigen (negative control) 3. 300 ul antiserum with 100 ul aldolase (2.5 mg/ml) 4. 300 ul antiserum with 200 ul aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum & an equal volume of aldolase antigen in PBS incubated 24 hrs at 4C, centrifuged for 6 mins at 13K RPM, washed once with PBS, centrifuged & dissolved in 60 ul 0.1 N NaOH. 90 ul of PBS was added, sample was divided in 2 portions, & an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer added. The reduced samples were boiled for five mins, & all samples were run at 140 V for 45 mins on a 4-20% tris/glycine gradient gel. Gel was stained, destained & imaged.")
![Western Blot: Aldolase A Antibody [NB600-915] - Figure. Immunoblotting. IgG purified antibody to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4 degrees C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Western-Blot-NB600-915-img0003.jpg)
![Western Blot: Aldolase A Antibody [NB600-915] - IgG purified antibody to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4 C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE).](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Western-Blot-NB600-915-img0009.jpg)
![Immunoprecipitation: Aldolase A Antibody [NB600-915] - Analysis using the Biotin conjugate of NB600-915. Detection of 300 ul of anti aldolase antiserum and an equal volume of varied amounts (diluted from a stock solution of ~2.5 ug/ml) of purified aldolase in PBS. Precipitation of aldolase was confirmed.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Immunoprecipitation-NB600-915-img0005.jpg)
![Western Blot: Aldolase A Antibody [NB600-915] - Twenty one more markedly oxidized proteins identified in 6‐week‐old PLP‐tg66/66 mice in the spinal cord. All non‐mitochondrial oxidized proteins tested by Western blot in the spinal cord are normally expressed. Relative protein level expressed as a percentage of control and in reference to gamma ‐tubulin as a loading marker (n = 6/genotype). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29027761/) licensed under a CC-BY license](http://images.novusbio.com/fullsize/nb600-915_goat-polyclonal-aldolase-a-antibody-2552023153959.jpg)
![Western Blot: EGFR Antibody [NB100-596] - NPM1 knockdown decreases EGF expression. NPM1 controls activation of the EGF/EGFR pathway downstream effectors. Proteins, extracted from shScr and shNPM1 LNCaP cells cultured in RPMI 1640 10%FBS, were electrophoresed by SDS-PAGE. Transferred membranes were immunoblotted with indicated antibodies. Histograms show the band quantification reported to the beta-actin level. Blots are representative of three independent experiments with consistent results. Data are representative of at least three independent experiments and are expressed as the mean +/- SD. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0096293), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EGFR-Antibody-Western-Blot-NB100-596-img0016.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR Antibody [NB100-596] - NPM1 knockdown decreases EGF expression. NPM1 controls activation of the EGF/EGFR pathway downstream effectors. Proteins, extracted from shScr and shNPM1 LNCaP cells cultured in RPMI 1640 10%FBS, were electrophoresed by SDS-PAGE. Transferred membranes were immunoblotted with indicated antibodies. Histograms show the band quantification reported to the beta-actin level. Blots are representative of three independent experiments with consistent results. Data are representative of at least three independent experiments and are expressed as the mean +/- SD. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0096293), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: EGFR Antibody [NB100-596] - Section of human placenta. Antibody: Affinity purified rabbit anti-EGFR used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NB100-596-img0013.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: EGFR Antibody [NB100-596] - EGF R/ErbB1 Antibody [NB100-596] - Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody:Affinity purified rabbit anti-EGFR antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/EGFR-Antibody-Western-Blot-NB100-596-img0015.jpg)
![Western Blot: EGFR Antibody [NB100-596] - Detection of Human EGFR on HeLa whole cell lysate using NB100-596.](http://images.novusbio.com/fullsize/EGFR-Antibody-Western-Blot-NB100-596-img0005.jpg)
![Western Blot: EGFR Antibody [NB100-596] - Detection of Human EGFR by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EGFR antibody NB100-596 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/EGFR-Antibody-Western-Blot-NB100-596-img0014.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: EGFR Antibody [NB100-596] - NPM1 knockdown in prostate cancer cells reduces proliferation and migration capacities by inhibiting the EGF/EGFR pathway activity.(a) NPM1 down-regulation inhibits EGF induced ERK1/2 pathway activity. shScr and shNPM1 LNCaP cells were treated with 100 nM EGF and phosphorylation of the EGF/EGFR pathway effectors was analyzed using Western Blotting. Histograms show the band quantification reported to the beta-actin level. The blot is representative of three independent experiments with consistent results. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0096293), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EGFR-Antibody-Western-Blot-NB100-596-img0017.jpg)