| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92458-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12692-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99239-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-721-100ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1545-0.025ml | |||
![Western Blot: AP2A2 Antibody [NBP2-92458] - Western blot analysis of extracts of various cell lines, using AP2A2 antibody (NBP2-92458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/AP2A2-Antibody-Western-Blot-NBP2-92458-img0003.jpg "Western Blot: AP2A2 Antibody [NBP2-92458] - Western blot analysis of extracts of various cell lines, using AP2A2 antibody (NBP2-92458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: AP2A2 Antibody [NBP2-92458] - Western blot analysis of extracts of SH-SY5Y cells, using AP2A2 antibody (NBP2-92458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/AP2A2 Antibody-Western Blot-NBP2-92458-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: AP2A2 Antibody - Azide and BSA Free [NBP2-92458] - Immunofluorescence analysis of NIH/3T3 cells using AP2A2 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92458_rabbit-polyclonal-ap2a2-antibody-42202514275253.jpg%3Ft=1690979160)
![Immunocytochemistry/ Immunofluorescence: AP2A2 Antibody - Azide and BSA Free [NBP2-92458] - Immunofluorescence analysis of HeLa cells using AP2A2 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92458_rabbit-polyclonal-ap2a2-antibody-42202514251886.jpg%3Ft=1690979177)
![Western Blot: AP2A2 Antibody [NBP3-12692] - Lane A: RT-4 Whole Cell LysateLane B: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:104 kDaObserved band size:104 kDa](http://images.novusbio.com/fullsize/AP2A2-Antibody-Western-Blot-NBP3-12692-img0001.jpg "Western Blot: AP2A2 Antibody [NBP3-12692] - Lane A: RT-4 Whole Cell LysateLane B: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:104 kDaObserved band size:104 kDa")
![Immunohistochemistry-Paraffin: AP2A2 Antibody [NBP3-12692] - Staining of human AP2A2 in human rectum with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/AP2A2-Antibody-Immunohistochemistry-Paraffin-NBP3-12692-img0003.jpg)
![Immunohistochemistry-Paraffin: AP2A2 Antibody [NBP3-12692] - Staining of human AP2A2 in human testis with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/AP2A2-Antibody-Immunohistochemistry-Paraffin-NBP3-12692-img0002.jpg)
![Immunoprecipitation: AP2A2 Antibody [NBP3-12692] - Lane A:0.5 mg RT-4 Whole Cell Lysate4 uL anti-AP2A2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-AP2A2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 104 kDaObserved band size :104 kDa](http://images.novusbio.com/fullsize/AP2A2-Antibody-Immunoprecipitation-NBP3-12692-img0004.jpg)
![Western Blot: AP2A2 Antibody (6D5F5) [NBP3-33317] - Western blot analysis of various lysates using AP2A2 Rabbit mAb at1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33317_rabbit-ap2a2-mab-6d5f5-101220241214473.jpg "Western Blot: AP2A2 Antibody (6D5F5) [NBP3-33317] - Western blot analysis of various lysates using AP2A2 Rabbit mAb at1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: AP2A2 Antibody [NBP2-21648] - Affinity Purified anti-AP2A antibody shows detection of a band just below 100 kDa corresponding to Human AP2A1 in a various preparations. Lane 1: HeLa nuclear extract, Lane 2: HeLa, Lane 3: 293, Lane 4: A431 and Lane 5: Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 ug/ml of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A. Approx. 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP2A antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection using a 60-sec exposure time.](http://images.novusbio.com/fullsize/AP2A2 Antibody-Western Blot-NBP2-21648-img0002.jpg "Western Blot: AP2A2 Antibody [NBP2-21648] - Affinity Purified anti-AP2A antibody shows detection of a band just below 100 kDa corresponding to Human AP2A1 in a various preparations. Lane 1: HeLa nuclear extract, Lane 2: HeLa, Lane 3: 293, Lane 4: A431 and Lane 5: Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 ug/ml of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A. Approx. 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP2A antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection using a 60-sec exposure time.")
![Western Blot: AP2A2 Antibody [NBP2-15417] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/AP2A2-Antibody-Western-Blot-NBP2-15417-img0001.jpg "Western Blot: AP2A2 Antibody [NBP2-15417] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE, antibody diluted at 1:500.")
![Western Blot: AP2A2 Antibody [NBP1-68948] - Human Lung lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/AP2A2-Antibody-Western-Blot-NBP1-68948-img0002.jpg "Western Blot: AP2A2 Antibody [NBP1-68948] - Human Lung lysate, concentration 0.2-1 ug/ml.")
![Western Blot: AP2A2 Overexpression Lysate (Adult Normal) [NBL1-07578] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AP2A2.](http://images.novusbio.com/fullsize/AP2A2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07578-img0002.jpg "Western Blot: AP2A2 Overexpression Lysate (Adult Normal) [NBL1-07578] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AP2A2.")
![Western Blot: AP2A2 Antibody [NBP2-99239] - Anti-AP2A2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: HeLa Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 104 kDa. Observed band size: 120 kDa](http://images.novusbio.com/fullsize/AP2A2-Antibody-Western-Blot-NBP2-99239-img0001.jpg "Western Blot: AP2A2 Antibody [NBP2-99239] - Anti-AP2A2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: HeLa Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 104 kDa. Observed band size: 120 kDa")
![Immunocytochemistry/Immunofluorescence: alpha Adaptin Antibody (AC1-M11) [NB300-721] - Shows immunolocalization of alpha-adaptin in NRK cells.](http://images.novusbio.com/fullsize/alpha-Adaptin-Antibody-AC1-M11-Immunocytochemistry-Immunofluorescence-NB300-721-img0006.jpg "Immunocytochemistry/Immunofluorescence: alpha Adaptin Antibody (AC1-M11) [NB300-721] - Shows immunolocalization of alpha-adaptin in NRK cells.")
![Immunohistochemistry-Paraffin: alpha Adaptin Antibody (AC1-M11) [NB300-721] - Immunohistochemistry on deparaffinized human heart tissue](http://images.novusbio.com/fullsize/alpha-Adaptin-Antibody-AC1-M11-Immunohistochemistry-Paraffin-NB300-721-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Adaptin Antibody (AP6) [NB600-1545] - alpha Adaptin (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/alpha-Adaptin-Antibody-AP6-Immunocytochemistry-Immunofluorescence-NB600-1545-img0003.jpg "Immunocytochemistry/Immunofluorescence: alpha Adaptin Antibody (AP6) [NB600-1545] - alpha Adaptin (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunocytochemistry/Immunofluorescence: alpha Adaptin Antibody (AP6) [NB600-1545] - alpha Adaptin (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/alpha-Adaptin-Antibody-AP6-Immunocytochemistry-Immunofluorescence-NB600-1545-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Adaptin Antibody (AP6) [NB600-1545] - alpha Adaptin (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/alpha-Adaptin-Antibody-AP6-Immunocytochemistry-Immunofluorescence-NB600-1545-img0002.jpg)