AP2A2 Antibody - BSA Free

Western Blot: AP2A2 Antibody [NBP2-15417] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE, antibody diluted at 1:500.

• Reactivity: Mu, Hu, Rt, Bv, Ch, Xp
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

AP2A2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit AP2A2 Antibody - BSA Free (NBP2-15417) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human AP2A2 protein. The exact sequence is proprietary.
• Localization: Cell membrane; Membrane; coated pit; Peripheral membrane protein; Cytoplasmic side; Cell membrane; Membrane; coated pit
• Predicted Species: Human (100%), Rat (99%), Chicken (98%), Xenopus (98%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AP2A2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 104 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (98%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for AP2A2 Antibody - BSA Free

• 100 kDa coated vesicle protein C
• Adapter-related protein complex 2 alpha-2 subunit
• adaptin, alpha B
• Adaptor protein complex AP-2 subunit alpha-2
• adaptor-related protein complex 2, alpha 2 subunit
• alpha2-adaptin
• Alpha-adaptin C
• alpha-adaptin C; Huntingtin interacting protein J
• AP-2 complex subunit alpha-2
• Clathrin assembly protein complex 2 alpha-C large chain
• clathrin-associated/assembly/adaptor protein, large, alpha 2
• DKFZP564D1864
• HIP9ADTAB
• Huntingtin yeast partner J
• Huntingtin-interacting protein 9
• Huntingtin-interacting protein J
• HYPJCLAPA2
• KIAA0899HIP-9
• Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit

Background

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: AP2A2
• Uniprot:
  • O94973(Human)