AP2A2 Antibody

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Western Blot: AP2A2 Antibody [NBP2-15417] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE, antibody diluted at 1:500.

Product Details

Summary
Reactivity Mu, Hu, Rt, Bv, Ch, XpSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.42 mg/ml

Order Details

AP2A2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Adaptin 2. The exact sequence is proprietary.
Localization
Cell membrane; Membrane; coated pit; Peripheral membrane protein; Cytoplasmic side; Cell membrane; Membrane; coated pit
Predicted Species
Human (100%), Rat (99%), Xenopus (98%), Bovine (98%), Chicken (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AP2A2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (98%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.42 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AP2A2 Antibody

  • 100 kDa coated vesicle protein C
  • Adapter-related protein complex 2 alpha-2 subunit
  • adaptin, alpha B
  • Adaptor protein complex AP-2 subunit alpha-2
  • adaptor-related protein complex 2, alpha 2 subunit
  • alpha2-adaptin
  • Alpha-adaptin C
  • alpha-adaptin C; Huntingtin interacting protein J
  • AP-2 complex subunit alpha-2
  • Clathrin assembly protein complex 2 alpha-C large chain
  • clathrin-associated/assembly/adaptor protein, large, alpha 2
  • DKFZP564D1864
  • HIP9ADTAB
  • Huntingtin yeast partner J
  • Huntingtin-interacting protein 9
  • Huntingtin-interacting protein J
  • HYPJCLAPA2
  • KIAA0899HIP-9
  • Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit

Background

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol AP2A2
Uniprot

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