AP2A2 Antibody - BSA Free

Western Blot: AP2A2 Antibody [NBP2-21648] - Affinity Purified anti-AP2A antibody shows detection of a band just below 100 kDa corresponding to Human AP2A1 in a various preparations. Lane 1: HeLa nuclear extract, Lane 2: HeLa, Lane 3: 293, Lane 4: A431 and Lane 5: Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 ug/ml of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A. Approx. 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP2A antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection using a 60-sec exposure time.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free

AP2A2 Antibody - BSA Free Summary

• Description: This affinity purified antibody is directed against human AP2A2. The product was affinity purified from antiserum by immunoaffinity purification
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified AP2A2 Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near aa 1-25 of Human AP2A1 and AP2A2 proteins. (Uniprot: O95782)
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AP2A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:20000
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:2000
• Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 104-107 kDa in size corresponding to AP2A1 or AP2A2 proteins by western blotting in the appropriate cell lysate or extract. Multiple isoforms of each subunit exist for these proteins. The immunogen sequence is found in both AP2A1 and AP2A2. This antibody recognizes a band of approximately 100 kDa in multiple human cell lines and specific reactivity can be blocked with the immunizing peptide.
• Control Peptide:

  AP2 alpha Protein (NBP1-96941)

Reactivity Notes

A BLAST analysis was used to suggest reactivity with AP2A21 and AP2A22 protein from human, chimpanzee, orangutan, mouse, rat, chicken and dog based on 100% homology for the immunogen sequence. Cross reactivity with AP2A2 proteins from other sources is not known.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for AP2A2 Antibody - BSA Free

• 100 kDa coated vesicle protein C
• Adapter-related protein complex 2 alpha-2 subunit
• adaptin, alpha B
• Adaptor protein complex AP-2 subunit alpha-2
• adaptor-related protein complex 2, alpha 2 subunit
• alpha2-adaptin
• Alpha-adaptin C
• alpha-adaptin C; Huntingtin interacting protein J
• AP-2 complex subunit alpha-2
• Clathrin assembly protein complex 2 alpha-C large chain
• clathrin-associated/assembly/adaptor protein, large, alpha 2
• DKFZP564D1864
• HIP9ADTAB
• Huntingtin yeast partner J
• Huntingtin-interacting protein 9
• Huntingtin-interacting protein J
• HYPJCLAPA2
• KIAA0899HIP-9
• Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit

Background

AP2A2 is a component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transportvia transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat componentsand appear to be involved in carg

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AP2A2
• Uniprot:
  • O95782(Human)