AP2A2 Antibody

Images

 
Western Blot: AP2A2 Antibody [NBP2-21648] - Affinity Purified anti-AP2A antibody shows detection of a band just below 100 kDa corresponding to Human AP2A1 in a various preparations. Lane 1: HeLa nuclear extract, Lane 2: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

AP2A2 Antibody Summary

Immunogen
Synthetic peptide corresponding aa 3-14 of Human AP2A1 and AP2A2 proteins. Protein accession number 27477041.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AP2A2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:5000-1:20000
  • Immunoprecipitation 1:100
Application Notes
This purified antibody has been tested for use in IP, ELISA and Western Blot. Expect a band approximately 104-107 kDa in size corresponding to AP2A1 or AP2A2 proteins by western blotting in the appropriate cell lysate or extract. Multiple isoforms of each subunit exist for these proteins. The immunogen sequence is found in both AP2A1 and AP2A2. This antibody recognizes a band of approximately 100 kDa in multiple human cell lines and specific reactivity can be blocked with the immunizing peptide.
Control Peptide
AP2 alpha Protein (NBP1-96941)

Reactivity Notes

Reactive in Human A BLAST analysis was used to suggest reactivity with AP2A1 and AP2A2 protein from Chimpanzee, Orangutan, Mouse, Rat, Chicken and Canine based on 100% identity for the immunogen sequence.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for AP2A2 Antibody

  • 100 kDa coated vesicle protein C
  • Adapter-related protein complex 2 alpha-2 subunit
  • adaptin, alpha B
  • Adaptor protein complex AP-2 subunit alpha-2
  • adaptor-related protein complex 2, alpha 2 subunit
  • alpha2-adaptin
  • Alpha-adaptin C
  • alpha-adaptin C; Huntingtin interacting protein J
  • AP-2 complex subunit alpha-2
  • Clathrin assembly protein complex 2 alpha-C large chain
  • clathrin-associated/assembly/adaptor protein, large, alpha 2
  • DKFZP564D1864
  • HIP9ADTAB
  • Huntingtin yeast partner J
  • Huntingtin-interacting protein 9
  • Huntingtin-interacting protein J
  • HYPJCLAPA2
  • KIAA0899HIP-9
  • Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol AP2A2
Uniprot