AP2A2 Antibody

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Western Blot: AP2A2 Antibody [NBP1-68948] - Human Lung lysate, concentration 0.2-1 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

AP2A2 Antibody Summary

Immunogen
Synthetic peptides corresponding to AP2A2 (adaptor-related protein complex 2, alpha 2 subunit) The peptide sequence was selected from the C terminal of AP2A2 (NP_036437). Peptide sequence LAKLKKKKGPSTVTDLEDTKRDRSVDVNGGPEPAPASTSAVSTPSPSADL.
Specificity
This product is specific to Subunit or Isofrom: alpha-2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AP2A2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2-1 ug/ml
Theoretical MW
104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for AP2A2 Antibody

  • 100 kDa coated vesicle protein C
  • Adapter-related protein complex 2 alpha-2 subunit
  • adaptin, alpha B
  • Adaptor protein complex AP-2 subunit alpha-2
  • adaptor-related protein complex 2, alpha 2 subunit
  • alpha2-adaptin
  • Alpha-adaptin C
  • alpha-adaptin C; Huntingtin interacting protein J
  • AP-2 complex subunit alpha-2
  • Clathrin assembly protein complex 2 alpha-C large chain
  • clathrin-associated/assembly/adaptor protein, large, alpha 2
  • DKFZP564D1864
  • HIP9ADTAB
  • Huntingtin yeast partner J
  • Huntingtin-interacting protein 9
  • Huntingtin-interacting protein J
  • HYPJCLAPA2
  • KIAA0899HIP-9
  • Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit

Background

AP2A2 is a component of the adaptor protein complex 2 (AP-2). AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol AP2A2
Entrez
Uniprot