| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1604-0.05ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48575-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48504-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58884-25ul | |||
![Immunocytochemistry/Immunofluorescence: Nestin Antibody [NB100-1604] - Characterization of MSCmix and NCSCmix isolated from the bone marrow of adult Wnt1-CRE/R26R-LacZ mice. NCSCmix express Nestin (green), Sox2 (red) Scale bar = 20 um. NCSC = neural crest stem cell, MSC = mesenchymal stem cell. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/6/1/211) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1604-img0008.jpg "Immunocytochemistry/Immunofluorescence: Nestin Antibody [NB100-1604] - Characterization of MSCmix and NCSCmix isolated from the bone marrow of adult Wnt1-CRE/R26R-LacZ mice. NCSCmix express Nestin (green), Sox2 (red) Scale bar = 20 um. NCSC = neural crest stem cell, MSC = mesenchymal stem cell. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/6/1/211) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Nestin Antibody [NB100-1604] - Characterization of MSCmix and NCSCmix isolated from the bone marrow of adult Wnt1-CRE/R26R-LacZ mice. MSCmix are adherent fibroblast-like cells, do not express Sox2 c (red), slightly express Nestin (c) (green). Scale bar = 20 um. MSC mesenchymal stem cell. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/6/1/211) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1604-img0007.jpg)
![Immunohistochemistry-Frozen: Nestin Antibody [NB100-1604] - Analysis of Nestin in frozen mouse tissue using anti-Nestin antibody. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Frozen-NB100-1604-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - In vitro proliferation & differentiation of ahMNCs-Clump II. (A) ahMNCs-Clump II were propagated in the culture condition for ahMNCs. The accumulated number of cells of ahMNCs-Clump II (NS18-007TL & 008TL) in comparison with ahMNCs established using previous culture methods (NS14-010TL, NS14-011TL, & NS15-001TL). Days to 1 × 109 cells are indicated. (B) Morphologies of ahMNCs-Clump II under expansion processes are illustrated until third in vitro passage (P3). Scale bar = 50 μm. (C) After in vitro differentiation, immunofluorescence was applied to ahMNCs-Clump II. Nestin for NSCs, MAP2 & Tuj1 for neurons, & GFAP for astrocytes. Undiffer = before in vitro differentiation; Differ = after in vitro differentiation. Scale bar = 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30380605), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1604_chicken-polyclonal-nestin-antibody-310202415291944.jpg)
![Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - Representative images of nestin (A) & Tmsb4x (B) staining in the mouse adult wild-type glomerulus visualized by fluorescent microscopy. (C) Merged images showing Tmsb4x (red) & nestin (green) staining; areas of colocalization are indicated by arrows. Representative images of Tmsb4x (D) & Cd31 (E) staining in the mouse adult wild-type glomerulus visualized by fluorescent microscopy. (F) Merged images showing Tmsb4x (green) & Cd31 (red) staining. Bar = 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27575556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1604_chicken-polyclonal-nestin-antibody-310202415304248.jpg)
![Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - Dopaminergic neuron derivation from H9 stem cells & hIPSCs.Scheme showing the different steps of the DA neuron derivation protocol (A). Immunolabelings of cultured H9 (top panels) & WT hiPSCs (bottom panels) for different NSCs markers, Sox1, Pax6 & Nestin (B). Immunolabeling of cultured H9 (top panels) & WT hiPSCs (bottom panels) after 3 days of DA progenitor induction for progenitor markers Lmx1A & FoxA2 (C) & after 7 days of induction for immature neurons beta III-Tubulin+ & remaining progenitors Sox1+ (D). Immunolabelings of cultured H9 (top panels) & WT hiPSCs (bottom panels) after 21 days of induction for the neuronal marqueur MAP2 & DA neurons Tyrosine Hydroxylase (TH) (E). Analysis of gene expression levels by qRT-PCR for cultured H9 (top panels) & WT hiPSCs at different time points (NSC, 14 days & 21 days) of the culture (FB = fetal brain) (F,G). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep33377), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1604_chicken-polyclonal-nestin-antibody-31020241555236.jpg)
![Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - Neural Stem Cell derivation from WT & LRRK2 G2019S hiPSCs.Haematoxylin & eosin coloration of teratoma-encapsulated tumors generated by flank injection of WT & G2019S hiPSCs cells in Nod/Scid mice. Representative images of the different germ layers: ectoderm (immature squamous epithelium, neural rosettes), mesoderm (primitive cartilage, muscles, fat) & endoderm (primitive gut like epithelium) (A). Scheme showing the different steps of the DA neuron derivation protocol (B). Immunolabelings of cultured WT & LRRK2 G2019S hIPSCs for different NSCs markers after NSCs induction protocol: Sox1, Nestin, Pax6 & Ki67 (C). Analysis of gene expression levels by qRT-PCR for WT & LRRK2 G2019S cultures at the end of the NSCs induction protocol (D). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep33377), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1604_chicken-polyclonal-nestin-antibody-31020241554536.jpg)
![Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - PAR1 is expressed by neural stem cells in the sub-ventricular zone (SVZ) of the adult mouse brain. Photomicrographs show immunofluorescence double-labeling for PAR1 with Sox2-positive (A), or PAR1 with Nestin-positive (C) multipotent neural stem cells (NSCs) within the lateral wall of the lateral ventricle (LV). RNAscope was used to identify cells expressing both PAR1 & Sox2 (B), or Nestin (D) RNA in NSCs of the adult SVZ. Arrow indicates an example of a double-labeled cell in each case, with arrowhead indicating a singly labeled cell (Scale bar = 10 μm). Boxed area in B & D is also shown at higher magnification to visualize double-labeled cells. (E) Histogram shows expression of PAR1 RNA was high in NSCs grown as neurospheres (NS), or when plated on poly-L-lysine coated coverslips as monolayers in stem cell media containing EGF & bFGF. PAR1 RNA expression by NSC monolayers decreased by 87% when EGF & bFGF were removed from the media for 7 DIV promoting stem cell differentiation. (F) Withdrawal of EGF & bFGF to induce NSC monolayer differentiation resulted in a parallel decrease in Nestin RNA expression. (**P < 0.01, ***P < 0.001 Students t-test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29921916), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1604_chicken-polyclonal-nestin-antibody-31020241612687.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human heart muscle, kidney, liver and lymph node using Anti-NES antibody NBP2-48575 (A) shows similar protein distribution across tissues to independent antibody NBP2-48504 (B).](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0010.jpg)
![Western Blot: Nestin Antibody [NBP2-48575] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line SK-MEL-30](http://images.novusbio.com/fullsize/Nestin-Antibody-Western-Blot-NBP2-48575-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody [NBP2-48575] - Staining of human cell line AF22 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48575-img0009.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human kidney using Anti-NES antibody NBP2-48575.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0013.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0006.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human heart muscle.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0011.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0012.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human liver using Anti-NES antibody NBP2-48575.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0014.jpg)
![Western Blot: Nestin Antibody [NBP2-48504] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NES antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Nestin-Antibody-Western-Blot-NBP2-48504-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Nestin Antibody [NBP2-48504] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NES antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human liver using Anti-NES antibody NBP2-48504.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0014.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human kidney shows distinct positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0005.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0008.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0010.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human heart muscle.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody [NBP2-58884] - Staining of human cell line RH-30 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58884-img0002.jpg "Immunocytochemistry/Immunofluorescence: Nestin Antibody [NBP2-58884] - Staining of human cell line RH-30 shows localization to intermediate filaments. Antibody staining is shown in green.")
![ELISA: Human Nestin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39115] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39115_human-nestin-ready-to-use-elisa-kit-colorimetric-2110202415343960.png "ELISA: Human Nestin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39115] - Standard Curve Reference")
