| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-171-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-30141SS | |||
| Azide and BSA Free | NBP2-80769 | |||
CTS013). Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Histone H2AX was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human Phospho-Histone H2AX (S139) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2288) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2288-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36468SS | |||
| Azide and BSA Free | NBP2-80770 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56346SS | |||
![Detection of Human and Mouse Histone H2AX [p Ser139] by Western Blot. Samples: Nuclear extract (50 ug) from human HEK293, human melanoma (G361), mouse wildtype embryonic fibroblasts (+/+) or mouse H2AX knockout embryonic fibroblasts (-/-). Antibody: Affinity purified rabbit Histone H2AX [p Ser139] antibody NB100-384 used at 0.1 ug/ml. Detection: Chemiluminescence with 30 second exposure. (NCS, neocarzinostatin - 200 ng/ml, 30 min). Bands appear at an observed molecular weight of ~15 kDa.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Western-Blot-NB100-384-img0012.jpg "<b>Genetic Strategies Validation. </b>Detection of Human and Mouse Histone H2AX [p Ser139] by Western Blot. Samples: Nuclear extract (50 ug) from human HEK293, human melanoma (G361), mouse wildtype embryonic fibroblasts (+/+) or mouse H2AX knockout embryonic fibroblasts (-/-). Antibody: Affinity purified rabbit Histone H2AX [p Ser139] antibody NB100-384 used at 0.1 ug/ml. Detection: Chemiluminescence with 30 second exposure. (NCS, neocarzinostatin - 200 ng/ml, 30 min). Bands appear at an observed molecular weight of ~15 kDa.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Samples: Neocarzinostatin treated asynchronous HeLa cells (left) and untreated asynchronous HeLa cells (right) . Antibody: Affinity purified rabbit Histone H2AX [p Ser139] used at a dilution of 1:5,000 (0.2ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight 594 used at a dilution of 1:100.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-384-img0009.jpg)
![Simple Western lane view shows a specific band for Histone H2AX [p Ser139] in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Simple-Western-NB100-384-img0014.jpg)
![<b>Biological Strategies Validation. </b>gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-NB100-384-img0024.jpg](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-NB100-384-img0024.jpg)
![gamma-H2AX-[p-Ser139]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-384-img0022.jpg](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-384-img0022.jpg)
![<b>Biological Strategies Validation. </b>Samples: Nuclear extract from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-). Antibody: Affinity purified rabbit Histone H2AX [p Ser139] antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Band appears at an observed molecular weight of ~17 kDa.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Western-Blot-NB100-384-img0016.jpg)
![<b>Biological Strategies Validation. </b>Detection of human Histone H2AX [p Ser139] by western blot. Samples: Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM EPE for 4 hours (+) or mock treated (-). Antibody: Affinity purified rabbit Histone H2AX [p Ser139] antibody NB100-384 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds. Band appears at an observed molecular weight of ~18 kDa.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Western-Blot-NB100-384-img0019.jpg)
![FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbi Histone H2AX [p Ser139] antibody used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-Paraffin-NB100-384-img0026.jpg)
![<b>Biological Strategies Validation. </b>Analysis of Histone H2AX [p Ser139] in EPE Treated Jurkat Cells. Cells were treated for 3 hrs in 5ug/ml etoposide, fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 million cells were stained with 0.5 ug anti-KLH or anti-H2AX NB100-384 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- etosposide treated, anti-KLH; Red- untreated, anti-Histone H2AX [p Ser139]; Blue- etoposide treated , anti-Histone H2AX [p Ser139].](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Flow-Cytometry-NB100-384-img0008.jpg)
![FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit Histone H2AX [p Ser139] antibody used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-Paraffin-NB100-384-img0025.jpg)
![Electropherogram image(s) of corresponding Simple Western lane view. Histone H2AX [p Ser139] antibody was used at 5 ug/ml dilution on Jurkat lysate(s).](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Simple-Western-NB100-384-img0015.jpg)
![Immunohistochemistry: Rabbit Polyclonal Histone H2AX [p Ser139] Antibody - Histone H2AX Antibody on mouse cancer tissue. H2AX (Gray) and H2BGFP(Green). Primary antibody dilution: 1:1000 in a 10um slice.](http://images.novusbio.com/fullsize/antibody/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-immunohistochemistry-16102023102936..png)
![Western Blot: Histone H2AX [p Ser139] Antibody [NB100-384] - ATM & NF-kappa B activation are downregulated in Ercc1-/ delta mice heterozygous for Atm. (A) Livers were collected at 12 weeks of age from WT, Ercc1-/ delta & Ercc1-/ delta Atm+/- mic (n=3 per genotype) & lysates analyzed by western blot for activation of ATM & its downstream effectors. (B) Same liver lysates were used to measure phosphorylation of p65 & I kappa B alpha . (C) Western blot analysis of livers from 16-week-old WT, Ercc1-/ delta & Ercc1-/ delta Atm+/-mice (n=3 per genotype) probed for activation of ATM. GAPDH was used as a loading control. (D) Same liver lysates used to measure activation of NF-kappa B. (E) Fourteen-week-old livers from Ercc1-/ delta & Ercc1-/ delta p65+/- mice (n=3 per genotype) were analyzed by western blot for activation of ATM (F) & NF-kappa B. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32201398), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-310202415371991.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-384] - Immunostaining of gamma H2AX, WT1 & 5mC in patients with IgA nephropathy & controls. Examples of PAS staining & immunostaining with gamma H2AX (green) & WT1 (red), pATM & 5mC in glomeruli of IgA nephropathy & controls. (A) A control kidney sample of 44-year-old female, (B) 65-year-old male of IgA nephropathy without podocytopathic features & (C) 55-year-old male of IgA nephropathy with podocytopathic features. Arrows indicate gamma H2AX & WT1 double-positive cells. Scale bars: 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31937846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-3102024153972.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-384] - UHRF1 deficiency resulted in impaired meiotic recombination & defective pachynema.a Double immunofluorescence of SYCP3 (green) & DMC1 (red) in testicular spread preparations. b, c The number of DMC1 foci in zygotene stage (b) & pachytene stage (c). d Immunostaining for SYCP3 (red) & gamma H2AX (green). e The percentage of abnormal gamma H2AX foci in the pachytene stage. f Immunostaining for SYCP3 (red) & MLH1 (green). g The number of MLH1 foci in pachynema. h Immunostaining for SYCP3 (red) & H1t (green). i The percentage of spermatocytes with H1T staining. ***p ≤ 0.001; *p ≤ 0.05. Scale bar, 5 μm in a, d, f, h. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32081844), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-310202415384166.jpg)
![Western Blot: Histone H2AX [p Ser139] Antibody [NB100-384] - mTORC1/2 activity prevents Cisplatin-induced cell death in MCF-10A cells. (A) Western blot displaying effects on mTOR signaling during a dose escalation of PP242 treatment in MCF-10A cells; (B) Western blot displaying effects of mTOR signaling on a dose escalation of cisplatin treatment in MCF-10A cells; (C) Western blot displaying effects on mTOR signaling & cell death during non-treated, Cisplatin, PP242, & Cisplatin + PP242-treated MCF-10A cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31771139), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-310202415304284.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-384] - Immunostaining of gamma H2AX, WT1 & 5mC in patients with IgA nephropathy & controls. Examples of PAS staining & immunostaining with gamma H2AX (green) & WT1 (red), pATM & 5mC in glomeruli of IgA nephropathy & controls. (A) A control kidney sample of 44-year-old female, (B) 65-year-old male of IgA nephropathy without podocytopathic features & (C) 55-year-old male of IgA nephropathy with podocytopathic features. Arrows indicate gamma H2AX & WT1 double-positive cells. Scale bars: 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31937846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-310202415371942.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-384] - Immunostaining of gamma H2AX, WT1 & 5mC in patients with IgA nephropathy & controls. Examples of PAS staining & immunostaining with gamma H2AX (green) & WT1 (red), pATM & 5mC in glomeruli of IgA nephropathy & controls. (A) A control kidney sample of 44-year-old female, (B) 65-year-old male of IgA nephropathy without podocytopathic features & (C) 55-year-old male of IgA nephropathy with podocytopathic features. Arrows indicate gamma H2AX & WT1 double-positive cells. Scale bars: 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31937846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-31020241534358.jpg)
![Western Blot: Histone H2AX [p Ser139] Antibody [NB100-384] - ATM & NF-kappa B activation are downregulated in Ercc1-/ delta mice heterozygous for Atm. (A) Livers were collected at 12 weeks of age from WT, Ercc1-/ delta & Ercc1-/ delta Atm+/- mic (n=3 per genotype) & lysates analyzed by western blot for activation of ATM & its downstream effectors. (B) Same liver lysates were used to measure phosphorylation of p65 & I kappa B alpha . (C) Western blot analysis of livers from 16-week-old WT, Ercc1-/ delta & Ercc1-/ delta Atm+/-mice (n=3 per genotype) probed for activation of ATM. GAPDH was used as a loading control. (D) Same liver lysates used to measure activation of NF-kappa B. (E) Fourteen-week-old livers from Ercc1-/ delta & Ercc1-/ delta p65+/- mice (n=3 per genotype) were analyzed by western blot for activation of ATM (F) & NF-kappa B. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32201398), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-3102024153565.jpg)
![Western Blot: Histone H2AX [p Ser139] Antibody [NB100-384] - Pharmacologic inhibition of ATM rescues oxidative stress-induced senescence by suppressing ATM- & NEMO-mediated NF-kappa B activation. (A) Representative images of primary WT & Ercc1-/- MEFs were induced to undergo senescence by serial passaging at 20% oxygen. At passage 5, MEFs were grown in the presence or absence of KU-55933 (10 μM) for 72 hrs. Senescence was determined by SA-beta gal staining. Images were obtained at the magnification of 10x. (B) Quantitation of the percent SA-beta gal positive cells. Graph represents the mean +/- s.e.m. of three independent experiments. Student’s t-test, ***p <0.001, ****p <0.0001. (C) Passage 5 Ercc1-/- MEFs treated with vehicle or KU-55933 (10 μM) for 72 hours were collected & levels of p21 & p16INK4a were determined by western blotting. (D) Passage 5 Ercc1-/- MEFs were treated with KU-55933 (10 μM) for 72 hours & whole cell lysate (CL) & nuclear extracts (NE) were analyzed by immunoblotting for expression of proteins involved in the DNA damage response. (E) Whole cell lysate (CL) & nuclear extract (NE) were extracted from Ercc1-/- MEFs treated with 10 μM of KU-55933 for analysis of nuclear NEMO & p65. GAPDH was used as a loading control of total proteins & LaminA/C as a loading control of nuclear protein. (F) Passage 5 WT & Ercc1-/- MEFs transfected with a NF-kappa B-luciferase reporter construct were cultured in the presence or absence of KU-55933 (10 μM) & were collected for luciferase assays after 72 hours. (G) qRT-PCR analysis of mRNA expression in passage 5 WT & Ercc1-/- MEFs treated with or without of KU-55933 (10 μM) for 72 hrs. P values were determined using a Student’s t-test. *p<0.05, **p<0.01, ***p <0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32201398), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-310202415525231.jpg)
![Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-384] - Orally administered lemongrass & white tea extract reduce tumor size in lymphoma xenograft model in immunocompromised miceImmunocompromised mice were subcutaneously injected with cancerous cells & tumors were allowed to establish. Treatments occurred every other day & the studied compound or the equivalent vehicle control administered orally for three weeks. (A, B) The tumors were photographed before & after extraction from the animals. (C) Tumor volume & mass were measured two times per week. (D) Immunohistochemistry analysis of sectioned tumor tissues from the lymphoma study. Each section was subjected to the specified antibody followed by a biotinylated secondary antibody. Detection was done using a DAB Peroxidase HRP Substrate Kit (brown) followed by Hematoxylin counterstaining (purple). Images were obtained using inverted bright field microscopy. Sectioning results are representative of three individual tumors. Scale bar is 50 microns. Statistical analysis using One-Way ANOVA. *p < 0.05 vs tumor volume of the control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22502), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-310202416572.jpg)
![Western Blot: Histone H2AX [p Ser139] Antibody [NB100-384] - CPT induces transcription- & proteasome-dependent apoptosis in quiescent WI38 hTERT cells. (A & B) Serum-starved cells were treated with DMSO or FLV (1 μM) for 1 h before the addition of DMSO or CPT (25 μM) for 16 & 24 h. (A) Percentages of cells that remained attached to culture flasks (means ± SD of quadruplicates). (B) Cell survival was analyzed by a CellTiter-Blue assay (means ± SD of triplicates). (C–G) Western blot of the indicated proteins in serum-starved cells treated for 1 h with DMSO or with FLV (1 μM) (C), lactacystin (10 μM) (D), veliparib (5 μM) or olaparib (10 μM) (E), ATMi (10 μM) (F) or DNA-PKi (10 μM) (G) before the addition of DMSO (‘-’, 24 h in panels C & F) or CPT (25 μM) for the indicated times. Data shown are representatives from two to three experiments. PARPCL: cleaved PARP. H2AX & alpha Tubulin were the loading controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26578593), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-310202415533820.jpg)
![Western Blot: Histone H2AX [p Ser139] Antibody [NB100-384] - Inhibition of DNA-PK prevents monoubiquitination of H2AX & H2A in CPT-treated quiescent WI38 hTERT cells. (A–E) Serum-starved cells were treated with DMSO or DNA-PKi (10 μM) for 1 h before the addition of DMSO (untreated) or CPT (25 μM) for 1 h. (A) Western blot of gamma H2AX. +Ub1 indicates gamma H2AX monoubiquitinated. The top panel shows quantification of Ub1-gamma H2AX normalized to alpha Tubulin (means ± SEM, n = 4). **P < 0.01. (B & C) Cells were pre-extracted with CSK buffer before co-staining for Ub-H2A (red) & 53BP1 phosphorylated on S1778 (p53BP1) (green). (B) Representative pictures. Images were merged to determine colocalization (yellow). The large Ub-H2AX focus at the periphery of the nuclei of untreated & CPT-treated cells may marks the inactive X chromosome as reported (91). (C) Percentages of nuclei with at least 5 Ub-H2A foci (means ± SEM, n = 3, 100 nuclei were analyzed for each treatment in each experiment). ***P < 0.001. (D & E) Cells were co-stained for ubiquitinated proteins (FK2, red) & gamma H2AX (green). (D) Representative pictures. Images were merged to determine colocalization (yellow). (E) Number of FK2 foci per nucleus from one representative experiment (76–111 nuclei were analyzed for each treatment) out of three. ****P < 0.0001. In the microscopic images, nuclear contours, identified by DAPI staining (blue in the merge images at bottom), are indicated by dashed lines. Bars: 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26578593), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-310202415541922.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-384] - The production of DSBs depends on Top1 degradation in CPT-treated quiescent cells. (A–C) Serum-starved WI38 hTERT cells were co-transfected with siRNAs against cullin 3 & cullin 4B or against a control sequence & then treated with DMSO (−CPT) or 25 μM CPT (+CPT) for 1 h. (A & B) Western blotting of the indicated proteins. alpha Tubulin: loading control. (C) Number of gamma H2AX foci per nucleus from one representative experiment (246–348 nuclei were analyzed for each treatment) out of three. ***P < 0.001. (D & E) Serum-starved WI38 hTERT cells were treated with DMSO or MG132 (50 μM) for 1 h before exposure to 0.8 Gy IR. One hour post-irradiation, cells were co-stained for gamma H2AX (green) & 53BP1 (red). (D) Representative pictures. (E) Number of gamma H2AX foci per nucleus from one representative experiment (162–180 nuclei were analyzed for each treatment) out of three. Ns: not significant. (F & G) U2OS EV28 cells were treated with DMSO or MG132 (10 μM) for 1 h before the addition of ethanol (untreated) or 300 nM 4-hydroxitamoxifen (4OHT) for 4 h to express AsiSI in the nucleus (42). (F) Representative pictures of cells co-stained for gamma H2AX (green) & 53BP1 (red). (G) ChIP analysis using an anti-gamma H2AX antibody (black) or a non-immune antibody (IgG, gray). Enrichment was assessed by QPCR amplification using primers proximal to two AsiSI sites located inside two genes (Gene I: SFRS6, Gene II: CCD47) & primers distal to an AsiSI site (Control). Enrichment was normalized to the maximum recovery for each experiment (means ± SEM, n = 3). Ns: not significant; *P < 0.05. In the microscopic images, nuclear contours, identified by DAPI staining (not shown), are indicated by dashed lines. Bars: 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26578593), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-310202415525243.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-384] - Inhibition of DNA-PK prevents monoubiquitination of H2AX & H2A in CPT-treated quiescent WI38 hTERT cells. (A–E) Serum-starved cells were treated with DMSO or DNA-PKi (10 μM) for 1 h before the addition of DMSO (untreated) or CPT (25 μM) for 1 h. (A) Western blot of gamma H2AX. +Ub1 indicates gamma H2AX monoubiquitinated. The top panel shows quantification of Ub1-gamma H2AX normalized to alpha Tubulin (means ± SEM, n = 4). **P < 0.01. (B & C) Cells were pre-extracted with CSK buffer before co-staining for Ub-H2A (red) & 53BP1 phosphorylated on S1778 (p53BP1) (green). (B) Representative pictures. Images were merged to determine colocalization (yellow). The large Ub-H2AX focus at the periphery of the nuclei of untreated & CPT-treated cells may marks the inactive X chromosome as reported (91). (C) Percentages of nuclei with at least 5 Ub-H2A foci (means ± SEM, n = 3, 100 nuclei were analyzed for each treatment in each experiment). ***P < 0.001. (D & E) Cells were co-stained for ubiquitinated proteins (FK2, red) & gamma H2AX (green). (D) Representative pictures. Images were merged to determine colocalization (yellow). (E) Number of FK2 foci per nucleus from one representative experiment (76–111 nuclei were analyzed for each treatment) out of three. ****P < 0.0001. In the microscopic images, nuclear contours, identified by DAPI staining (blue in the merge images at bottom), are indicated by dashed lines. Bars: 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26578593), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-31020241682137.jpg)
![Western Blot: Histone H2AX [p Ser139] Antibody [NB100-384] - DDR & NF-kappa B are activated concomitantly in senescent MEFs & aged tissues. (A) Immunoblot detection of p-p65 & total p65 in liver tissue from 16-week-old WT (n=3) & Ercc1-/ delta (n=3) mice. (B) Immunoblot detection of phosphorylation of ATM & downstream targets gamma H2AX & p21 in liver from 16-week-old WT & Ercc1-/ delta mice. (C) Immunoblot detection of phosphorylation of NF-kappa B & I kappa B alpha in liver lysates from 3, 12 & 24 month-old WT mice. n=3 mice per group. (D) Immunoblot detection of p-ATM, ATM & p21 in the same liver lysates. (E) Immunoblot detection of DDR effectors in nuclear extracts from passage 5 WT & Ercc1-/- MEFs, grown at 20% oxygen. (F) Level of NF-kappa B activation is higher in Ercc1-/- MEFs compared to WT MEFs at passage 5, as measured by Immunoblot detection of p-p65 & total p65 in WT & Ercc1-/- MEFs at passage 5 after culturing in 20% oxygen. (G) Representative images of immunofluorescent detection of p65 & NEMO in passage 4 WT & Ercc1-/- MEFs grown at 20% oxygen. Blue: DAPI staining; Green: p65 (top panel) or NEMO (bottom panel). Images were taken at the magnification of 60x. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32201398), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-3102024165718.jpg)
![Western Blot: Histone H2AX [p Ser139] Antibody [NB100-384] - DDR & NF-kappa B are activated concomitantly in senescent MEFs & aged tissues. (A) Immunoblot detection of p-p65 & total p65 in liver tissue from 16-week-old WT (n=3) & Ercc1-/ delta (n=3) mice. (B) Immunoblot detection of phosphorylation of ATM & downstream targets gamma H2AX & p21 in liver from 16-week-old WT & Ercc1-/ delta mice. (C) Immunoblot detection of phosphorylation of NF-kappa B & I kappa B alpha in liver lysates from 3, 12 & 24 month-old WT mice. n=3 mice per group. (D) Immunoblot detection of p-ATM, ATM & p21 in the same liver lysates. (E) Immunoblot detection of DDR effectors in nuclear extracts from passage 5 WT & Ercc1-/- MEFs, grown at 20% oxygen. (F) Level of NF-kappa B activation is higher in Ercc1-/- MEFs compared to WT MEFs at passage 5, as measured by Immunoblot detection of p-p65 & total p65 in WT & Ercc1-/- MEFs at passage 5 after culturing in 20% oxygen. (G) Representative images of immunofluorescent detection of p65 & NEMO in passage 4 WT & Ercc1-/- MEFs grown at 20% oxygen. Blue: DAPI staining; Green: p65 (top panel) or NEMO (bottom panel). Images were taken at the magnification of 60x. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32201398), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-31020241682193.jpg)
![Western Blot: Histone H2AX [p Ser139] Antibody [NB100-384] - Induction of ubiquitin/proteasome-dependent DSBs in CPT-treated quiescent WI38 hTERT cells. (A–F) Serum-starved cells were treated with DMSO (1 h) or with MG132 (50 μM, 1 h), lactacystin (10 μM, 1 h), bortezomib (1 μM, 4 h), G5 (1.5 μM, 0.5 h) or Pyr-41 (9 μM, 0.5 h) before the addition of DMSO (untreated) or 25 μM CPT for 1 h & then co-stained for gamma H2AX (green) & 53BP1 (red) or analyzed by Western blot. ‘-’ in panels C & F means cells treated with DMSO. (A & D) Representative pictures. Nuclear contours, identified by DAPI staining (not shown), are indicated by dashed lines. Bars: 10 μm. (B & E) Number of gamma H2AX foci per nucleus from two independent experiments (147–153 nuclei were analyzed for each treatment). ****P < 0.0001. (C & F) Western blot of gamma H2AX. alpha Tubulin: loading control. Dashed lines indicate that intervening wells have been spliced out. The top panels show quantification of gamma H2AX normalized to alpha Tubulin (means ± SEM, n = 4 in panel C, n = 3 in panel F). ***P < 0.001; **P < 0.01. (G & H) Detection of DSBs by neutral Comet assays in serum-starved cells treated with DMSO or MG132 (25 μM) for 1 h before the addition of DMSO (untreated) or CPT for 1 h (7.5 μM for experiments (Exp) I & II; 5 & 7.5 μM for Exp III). (G) Representative pictures of nuclei from Exp I. (H) Quantification of neutral Comet tail moments for three independent experiments (95–133 nuclei were analyzed for each treatment in each experiment). ***P < 0.001; ****P < 0.0001. The untreated & CPT data from Exp I are from the same experiment as that of Supplementary Figure S3D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26578593), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-310202415541946.jpg)
![Western Blot: Histone H2AX [p Ser139] Antibody [NB100-384] - Curcumin Analogs Induce Apoptosis in Cancerous Cells by Several Pathways. (a) E6-1 (Jurkat), dominant negative FADD (dnFADD) Jurkat, & overexpressing BCL-2 Jurkat were treated for 48 hours then stained for Annexin V & PI (b) E6-1 cells were plated & treated with or without the broad spectrum caspase inhibitor ZVAD(oMe)-FMK for 48 hours. Cells were stained for Annexin V & PI. Results were obtained using image-based cytometry with the Y-axis representative of percent of cells positive for Annexin V (green), PI (red), Annexin V & PI (yellow), or negative for both Annexin V & PI (blue). Values are expressed as a mean ± SD from three independent experiments. (c) E6-1 cells were treated for 24 hours with or without the broad spectrum caspase inhibitor ZVAD(oMe)-FMK & the studied compounds, lysed & subjected to Western blot analysis. (d) NHF & NCM460 cells were treated for 48 hours & 72 hours respectively, lysed & subjected to Western blot analysis. Bands were visualized with a chemiluminescence reagent. Images are representative of three independent experiments. Statistical calculations were performed using Two-Way ANOVA multiple comparison. *p < 0.05 vs % viable of Control (DMSO); #p < 0.05 vs % viable cells for groups without Z-VAD(oMe)FMK. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28439094), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-31020241612653.jpg)
![Western Blot: Histone H2AX [p Ser139] Antibody [NB100-384] - Curcumin Analogs Induce Apoptosis in Cancerous Cells by Several Pathways. (a) E6-1 (Jurkat), dominant negative FADD (dnFADD) Jurkat, & overexpressing BCL-2 Jurkat were treated for 48 hours then stained for Annexin V & PI (b) E6-1 cells were plated & treated with or without the broad spectrum caspase inhibitor ZVAD(oMe)-FMK for 48 hours. Cells were stained for Annexin V & PI. Results were obtained using image-based cytometry with the Y-axis representative of percent of cells positive for Annexin V (green), PI (red), Annexin V & PI (yellow), or negative for both Annexin V & PI (blue). Values are expressed as a mean ± SD from three independent experiments. (c) E6-1 cells were treated for 24 hours with or without the broad spectrum caspase inhibitor ZVAD(oMe)-FMK & the studied compounds, lysed & subjected to Western blot analysis. (d) NHF & NCM460 cells were treated for 48 hours & 72 hours respectively, lysed & subjected to Western blot analysis. Bands were visualized with a chemiluminescence reagent. Images are representative of three independent experiments. Statistical calculations were performed using Two-Way ANOVA multiple comparison. *p < 0.05 vs % viable of Control (DMSO); #p < 0.05 vs % viable cells for groups without Z-VAD(oMe)FMK. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28439094), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-31020241683932.jpg)
![gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-NB100-2280-img0022.jpg](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-NB100-2280-img0022.jpg "gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-NB100-2280-img0022.jpg")
![Simple Western: Histone H2AX [p Ser139] Antibody [NB100-2280] - Simple Western lane view shows a specific band for Histone H2AX [p Ser139] in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Simple-Western-NB100-2280-img0008.jpg)
![gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-NB100-2280-img0021.jpg](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-NB100-2280-img0021.jpg)
![Immunohistochemistry-Paraffin: Histone H2AX [p Ser139] Antibody [NB100-2280] - Detection of human Histone H2AX [p Ser139] antibody by immunohistochemistry. Sample: FFPE section of human seminoma. Antibodies: Affinity purified rabbit Histone H2AX [p Ser139] antibody used at a dilution of 1:500. Detection: DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-Paraffin-NB100-2280-img0023.jpg)
![Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Detection of Mouse Histone H2AX [p Ser139] by Immunohistochemistry. Sample: FFPE section of mouse colon carcinoma CT26. Antibodies: Affinity purified rabbit Histone H2AX [p Ser139] antibody. Detection: DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-NB100-2280-img0018.jpg)
![Simple Western: Histone H2AX [p Ser139] Antibody [NB100-2280] - Electropherogram image(s) of corresponding Simple Western lane view. Histone H2AX [p Ser139] antibody was used at 1:20 dilution on Jurkat lysate(s).](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Simple-Western-NB100-2280-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-2280] - Treatment with anti-thyroid drug protected RPE & photoreceptors from oxidative damage induced by NaIO3.RPE & retinal oxidative damage were evaluated by immunofluorescence labeling of p-gamma H2AX & 8-OHdG on the RPE whole mounts & retinal sections at 3 days post-NaIO3 injection. a Shown are representative images of p-gamma H2AX immunofluorescence labeling on the RPE whole mounts. b Shown are representative images of p-gamma H2AX & 8-OHdG immunofluorescence labeling on the retinal sections, & corresponding quantitative analysis for p-gamma H2AX labeling. ONL, outer nuclear layer; INL, inner nuclear layer. Data represented the mean ± SEM for 5 mice per group (*p < 0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31932580), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-310202415175243.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-2280] - Treatment with anti-thyroid drug protected RPE & photoreceptors from oxidative damage induced by NaIO3.RPE & retinal oxidative damage were evaluated by immunofluorescence labeling of p-gamma H2AX & 8-OHdG on the RPE whole mounts & retinal sections at 3 days post-NaIO3 injection. a Shown are representative images of p-gamma H2AX immunofluorescence labeling on the RPE whole mounts. b Shown are representative images of p-gamma H2AX & 8-OHdG immunofluorescence labeling on the retinal sections, & corresponding quantitative analysis for p-gamma H2AX labeling. ONL, outer nuclear layer; INL, inner nuclear layer. Data represented the mean ± SEM for 5 mice per group (*p < 0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31932580), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-310202415171325.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-2280] - RES rescued DXR-induced apoptosis through DNA-damage-P63-Caspase3 pathway in mouse oocytes. (A) Representative immunofluorescence images showing the expression of gamma -H2AX in mouse oocytes. Green, gamma -H2AX, Blue, DNA, Bar = 20 μm. (B) The relative immunofluorescence intensity of gamma -H2AX was measured in control, DXR-treated & RES-supplemented oocytes. Experiments were repeated at least 3 times with more than 30 oocytes examined for each group. Data were presented as means ± S.E.M of three independent experiments. **means P < 0.01, *** means P < 0.001. (C) Protein levels of gamma -H2AX, P63 & Active-Caspase3 were examined by Western blotting in control, DXR-treated & RES-supplemented oocytes. GAPDH was used as a loading control. The clean backgrounds for the active-Caspase-3, gamma -H2AX & GAPDH is due to the exposure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32352929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-3102024165725.jpg)
![Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Hepatic activation of FOXO3 induces oxidative damage & Akt activation. a Expression of Bcl2l11 (Bim), Sesn2 & Sesn3 by qPCR in livers from 9-week-old control & FOXO3CAHep transgenic mice (n = 4). b Representative IHC staining for 8-OHdG (bar = 100 μm) & quantification of 8-OHdG-positive cells as a percentage of total hepatocyte cells in livers of patients in area of small cells (SC) & large cells (LC). c Representative IHC staining for phospho-gamma H2AX (bar = 100 μm) & quantification of phospho-gamma H2AX-positive cells as a percentage of total hepatocyte cells in livers of patients in area of small cells & large cells. d Expression of Msh2 by qPCR (upper panel), Western blot analysis for MSH2 (middle panel) & densitometric quantification (lower panel) in livers from 9-week-old control & FOXO3CAHep transgenic mice (n = 4). e Western blot analysis in livers from 9-week-old control & FOXO3CAHep mice for serine-473 phospho-Akt, with Akt & GAPDH as loading control (left panel), as well as densitometric quantification (right panel). f Representative IHC staining for phospho-Akt in livers from 9-week-old control & FOXO3CAHep mice (bar = 100 μm). g Western blot analysis in livers from 9-week-old control & FOXO3CAHep mice for Rictor & GAPDH as loading control (left panel), as well as densitometric quantification (right panel) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31488102), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-310202415541940.jpg)
![Western Blot: Histone H2AX [p Ser139] Antibody [NB100-2280] - RES rescued DXR-induced apoptosis through DNA-damage-P63-Caspase3 pathway in mouse oocytes. (A) Representative immunofluorescence images showing the expression of gamma -H2AX in mouse oocytes. Green, gamma -H2AX, Blue, DNA, Bar = 20 μm. (B) The relative immunofluorescence intensity of gamma -H2AX was measured in control, DXR-treated & RES-supplemented oocytes. Experiments were repeated at least 3 times with more than 30 oocytes examined for each group. Data were presented as means ± S.E.M of three independent experiments. **means P < 0.01, *** means P < 0.001. (C) Protein levels of gamma -H2AX, P63 & Active-Caspase3 were examined by Western blotting in control, DXR-treated & RES-supplemented oocytes. GAPDH was used as a loading control. The clean backgrounds for the active-Caspase-3, gamma -H2AX & GAPDH is due to the exposure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32352929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-31020241554196.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-2280] - Oocyte-specific deletion of Gsk-3 beta disrupted early folliculogenesis in mice. aGsk-3 beta efficiently & specifically deleted in the oocytes in cKO mouse ovary. Immunofluorescence staining for GSK-3 beta (green) & DDX4 (red) on 1 dpp ovary. The nucleus stained by Hoechst (blue). b Control & cKO ovaries at the indicated developmental stages. Oocytes stained w/ DDX4 (green). The nucleus stained by Hoechst (blue). c Statistical analysis showed that the total number of primordial follicle decreased significantly in 7 dpp cKO ovaries (Additional file 8: Individual data values). d Apoptotic cells increased in 1 dpp cKO ovaries compared w/ the control ovaries. TUNEL signals (green) marked apoptotic cells. The nucleus stained by Hoechst (blue). e DSBs persisted in the oocytes of 1 dpp cKO ovaries. The sections stained w/ gamma -H2AX (green) & DDX4 (red). The nucleus stained by Hoechst (blue). f Ectopic RAD51 expression in the oocytes of 1 dpp cKO ovaries. The sections stained w/ RAD51 (green) & DDX4 (red). The nucleus stained by Hoechst (blue). g, h beta -catenin accumulated in the cytoplasm & translocated into nucleus of the oocytes in cKO mice. g The sections stained w/ beta -catenin (green) & DDX4 (red). The nucleus stained by Hoechst (blue). Arrowheads indicated oocytes showing nuclear beta -catenin accumulation. h The statistic analysis demonstrated that the percentage of oocytes showing beta -catenin accumulation per section increased significantly in cKO mice (Additional file 8: Individual data values). The data are presented as mean ± s.d. The asterisk (*) denotes a statistically significant difference between the control & treatment groups. *P < 0.05, **P < 0.01, & ***P < 0.001 (t test). Scale bars, 200 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30866939), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-3102024165746.jpg)
![Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - IDO1 inhibitor enhances the efficacy of radiotherapy in vivo. 1 × 105 of SiHa tumorsphere cells were subcutaneously injected to nude mice for tumor growth. After the tumor volume reached 50 mm3, the mice were divided into four groups of non-treated (Ctrl), INCB-024360 treated (INCB), radiotherapy (Rx), or INCB-024360 plus radiotherapy (INCB + Rx). For the INCB or INCB + Rx group, mice were injected once with 50 mg/kg INCB-024360 intraperitoneally before radiotherapy. For the Rx or INCB + Rx group, mice received 2 Gy radiation per day for total 10 Gy. Mice were sacrificed at day 30 after the last radiation treatment & the xenografted tumors were taken out for picturing (A) & weighting (B). The expression of Ki-67 or p-gamma H2AXser139 was determined by paraffin section followed by immunohistochemical staining (C). The inserted bars indicated 50 μM. The quantification results were performed by TissueFAX software (D). * p < 0.05; ** p < 0.01. The experiments were repeated two times & data from one experiment were presented. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32545442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-31020241612641.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-2280] - Premature upregulation of TAp63 in GSK-3 beta -inhibited ovary. a Expression pattern of gamma -H2AX in fetal & neonatal mouse ovary in vivo. Mouse ovaries from 13.5 dpc, 15.5 dpc, 17.5 dpc, & 1 dpp immunostained for gamma -H2AX (green) & DDX4 (red). Nucleus stained by Hoechst (blue). gamma -H2AX displayed intensive expression in germ cell nucleus from 15.5 to 17.5 dpc. b Expression pattern of TAp63 in fetal & neonatal mouse ovary in vivo. Mouse ovaries from 13.5 dpc, 15.5 dpc, 17.5 dpc, 18.5 dpc, & 1 dpp immunostained for TAp63 (green) & DDX4 (red). Nucleus stained by Hoechst (blue). TAp63 protein located w/in somatic cells in fetal ovary & began to express in germ cell nucleus from 18.5 dpc afterward. c–e TAp63 expression upregulated in fetal ovary & displayed premature localization w/in oocyte nucleus following GSK-3 beta inhibition. Before examination, ovaries of 14.5 dpc cultured in vitro w/ DMSO or BIO for 3 days. c qRT-PCR analysis of TAp63 mRNA level following GSK-3 beta inhibition (normalized to beta -actin) (Additional file 8: Individual data values). d WB analysis of TAp63 protein level following GSK-3 beta inhibition. GAPDH used as internal control. e Immunofluorescence staining for TAp63 (green) & DDX4 (red). The nucleus stained by Hoechst (blue). TAp63 showed premature expression w/in oocyte nucleus following GSK-3 beta inhibition (arrowhead). f qRT-PCR results showed that relative mRNA expression level of p21, Bad, & Noxa increased significantly in GSK-3 beta -inhibiting ovaries (Additional file 8: Individual data values). data are presented as mean ± s.d. The asterisk (*) denotes a statistically significant difference between the control & treatment groups. *P < 0.05, **P < 0.01, & ***P < 0.001 (t test). Scale bars, 200 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30866939), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-31020241612651.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - Analysis of HeLa, COS, C6, and K562 cell lysate using histone H3 antibody [NB500-171] antibody at 1:100. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0013.jpg "Western Blot: Histone H3 Antibody [NB500-171] - Analysis of HeLa, COS, C6, and K562 cell lysate using histone H3 antibody [NB500-171] antibody at 1:100. Observed molecular weight is ~17 kDa.")
![Chromatin Immunoprecipitation: Histone H3 Antibody [NB500-171] - Chromatin from one million formaldehyde cross-linked HeLa cells was precipitated using 2 ug of NB500-171 and 25 uL of magnetic IgG beads, using standard ChIP methods. A similar sample containing no antibody (No Ab) was included as a negative control. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin. Representative target genes from active, inactive, and heterochromatic regions of the genome show amplification, indicative of the presence of Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Chromatin-Immunoprecipitation-NB500-171-img0016.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE mouse testis.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0017.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Histone H3 Antibody [NB500-171] - MDA-MB-231 cells were treated with vehicle (V) or paclitaxel (P). Cytosolic and nuclear lysates were prepared, and immunoblot assay was performed. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0020.jpg)
![Immunohistochemistry: Histone H3 Antibody [NB500-171] - Analysis of Histone H3 in human breast cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-NB500-171-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB500-171 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB500-171 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - Immunofluorescence staining to detect histone H3 in human blood. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0023.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Analysis of Histone H3 antibody on Mouse pancreas tissue. Histone H3 antibody dilution 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0025.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - (1) HeLa, (2) S. cerevisiae whole cell lysates, (3) Histones purified from HeLa cells. Theoretical molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0014.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - Total histone H3 levels in MCF-7 and HCT-116 cells. Observed molecular weight is ~17 kDa. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 at 5 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 at 5 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0019.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE rat pancreas.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0009.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE rat colon.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0010.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE mouse brain.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0007.jpg)
![Western Blot: Histone H3 Antibody - BSA Free [NB500-171] - p53 but not ATM is required for chronic gamma -radiation-induced histone reductions. (a) Western blots of histone proteins in wild-type, TP53−/− & ATM−/− RPE-1 cells following exposures to 20 mGy/h chronic radiation for 7 days. (b) ATM activation, as shown by auto-phosphorylation on S1981, in response to chronic radiation in primary fibroblasts. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-171_rabbit-polyclonal-histone-h3-antibody-31020241539715.jpg)
![Western Blot: Histone H3 Antibody - BSA Free [NB500-171] - Reduced histone levels in senescent cells is induced in vitro by different means, & in vivo from aged donors. (a) Phase-contrast images of primary fibroblasts induced into senescence by chronic gamma -radiation, oncogene over-expression or exhaustive replication (replicative senescence), & DNA damage from a single acute 4 Gy X-ray dose at an early time-point (1 hour) as a control for DNA damage without senescence. Scale bars 200 µm. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a & b) & adult donors. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-171_rabbit-polyclonal-histone-h3-antibody-31020241534368.jpg)
![Western Blot: Histone H3 Antibody - BSA Free [NB500-171] - Reduced histone levels in senescent cells is induced in vitro by different means, & in vivo from aged donors. (a) Phase-contrast images of primary fibroblasts induced into senescence by chronic gamma -radiation, oncogene over-expression or exhaustive replication (replicative senescence), & DNA damage from a single acute 4 Gy X-ray dose at an early time-point (1 hour) as a control for DNA damage without senescence. Scale bars 200 µm. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a & b) & adult donors. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-171_rabbit-polyclonal-histone-h3-antibody-310202415291940.jpg)
![Western Blot: Histone H3 Antibody - BSA Free [NB500-171] - Chronic gamma -radiation reduces histone levels. (a) Western blot analyses of histone H2AX & gamma H2AX in primary fibroblasts exposed to various dose-rates of chronic gamma -radiation for 7 days. Cells irradiated with a single acute dose of 4 Gy X-ray were included as control. (b) Effect of chronic gamma -irradiation on H2AX levels in three different isogenic primary cell types from a different donor to that used in (a) at the same dose rates as in (a). (c) Immunoblots of other histones in chronically irradiated primary fibroblasts. (d) All significant histone level changes detected by SILAC LC-MS/MS protein analyses of samples from primary fibroblasts exposed or mock-exposed to chronic gamma -radiation for 7 days. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-171_rabbit-polyclonal-histone-h3-antibody-310202415392548.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0012.jpg "Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.")
![Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Histone 3 was detected in immersion fixed paraffin-embedded sections of human colon cancer using anti-human mouse monoclonal antibody (Catalog # NBP1-30141) at 1:1000 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Immunohistochemistry-Paraffin-NBP1-30141-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 [Trymethyl Lys9] (6F12-H4) at 2 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (green) at a 1:500 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Immunocytochemistry-Immunofluorescence-NBP1-30141-img0011.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 (NBP1-30141) using HeLa nuclear lysate [NB800-PC9]. Observed molecular weight ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of A549 cells (fixed with methanol) using Histone H3 [Trimethyl Lys9] antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Immunocytochemistry-Immunofluorescence-NBP1-30141-img0014.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Used to perform ChIP on HeLa cells.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Chromatin-Immunoprecipitation-NBP1-30141-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - H3K9Me3 analysis of MCF7 and HCT116. Theorectical molecular weight is ~15 kDa. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0008.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 K9-me3 in HeLa histone lysates. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0004.jpg)
![Histone H3 [Trimethyl Lys9] (6F12-H4) was detected in immersion fixed MCF7 human breast cancer cell line using Mouse anti- Histone H3 [Trimethyl Lys9] (6F12-H4) Protein G Purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NBP1-30141R) (red) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/antibody/nbp1-30141_mouse-monoclonal-histone-h3-trimethyl-lys9-antibody-6f12-h4-immunocytochemistry-immunofluorescence-2382024134224..jpg)
![Simple Western: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Simple Western lane view shows a specific band for Histone H2AX [p Ser139] in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Simple-Western-NB100-74435-img0012.jpg "Simple Western: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Simple Western lane view shows a specific band for Histone H2AX [p Ser139] in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Staining of Phospho-H2AX in A549 cells. Cells were treated with 50 uM EPE for 1 hour (left panel) or with vehicle (right control: 0.1% DMSO in media).](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0009.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Staining using NB100-74435, treatment with paraquat and iron induces MnSOD and Phosphorylation of H2AX in RAW 264.7 macrophages. Cells were treated for 20 hours with paraquat (500 uM) and iron (200 ug/ml) and stained with anti-Phospho-H2AX antibody.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0008.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Western blot analysis of Jurkat cells (Lane 1) and Jurkat cells treated with staurosporine (Lane 2) blotted with Histone H2AX [p Ser139] antibody (3F2). Bands indicate an observed molecular weight of ~16 kDa.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Western-Blot-NB100-74435-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - STED image of MDA-MB-231 cells after 2 Gy irradiation. Blue channel: DAPI, window level [0 255]. Green channel: secondary antibody Alexa Fluor 488, window level [140 255]. Scale bar: 20 um. Image from verified customer review.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Immunofluorescence analysis using Histone H2AX [p Ser139] antibody (3F2).](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - IF image of phosphorylated H2AX in gamma irradiated Hela cells.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0015.jpg)
![Simple Western: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Electropherogram image(s) of corresponding Simple Western lane view. Histone H2AX [p Ser139] antibody (3F2) was used at 10 ug/ml dilution on Jurkat lysate(s).](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Simple-Western-NB100-74435-img0013.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Evidence of DNA damage & oxidative stress in islets from HFD treated mice. (a) Representative immunofluorescent staining of gamma H2AX, insulin, & DAPI for islets from each treatment group. (b) Representative immunofluorescent staining of 4-hydroxynonenal (4-HNE), insulin, & DAPI for islets from each treatment group. (c) Representative immunofluorescent staining of nitrotyrosine, insulin, & DAPI for islets from each treatment group. (d–g) qRT-PCR analysis of total islet RNA for antioxidant genes, including Gpx1, SOD1, Nrf2, & Ppargc1 alpha . (n = 3) *p < 0.05, **p < 0.01. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-03869-5), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74435_mouse-monoclonal-histone-h2ax-p-ser139-antibody-3f2-31020241534396.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Citrulline 2, Citrulline 8, Citrulline 17] Antibody [NB100-57135] - HL60 cells treated with DMSO and Calcimycin Green: (at 1:1000 Blue Hoechst)](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-57135-img0004.jpg "<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H3 [Citrulline 2, Citrulline 8, Citrulline 17] Antibody [NB100-57135] - HL60 cells treated with DMSO and Calcimycin Green: (at 1:1000 Blue Hoechst)")
![<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [Citrulline 2, Citrulline 8, Citrulline 17] Antibody [NB100-57135] - Histone H3/Citrulline (R2,R8,R17) at 1:10000 dilution. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Citrulline-2-Citrulline-8-Citrulline-17]-Antibody-Western-Blot-NB100-57135-img0003.jpg)
![Immunohistochemistry: Rabbit Polyclonal Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody [NB100-57135] - Analysis of Histone H3 on mouse brain tissue. Nucleus (Blue, DAPI) ; H3cit (green). Hemorrhagic stroke model FxFr ; 10 µm thickness 1:400 Secondary antibody AF488. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-57135_rabbit-polyclonal-histone-h3-antibody-immunohistochemistry-20320259417..png)
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody [NB100-638] - Asynchronous wild-type (+/+) and H2AX knockout (-/-) mouse embryonic fibroblasts. Antibody used at 2 ug/ml.](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-Immunocytochemistry-Immunofluorescence-NB100-638-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Histone H2AX Antibody [NB100-638] - Human 293T and wild-type (+/+) or H2AX knockout (-/-) mouse embryonic fibroblasts. Antibody used at the indicated concentrations.](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-Western-Blot-NB100-638-img0004.jpg)
![Western Blot: Histone H2AX Antibody [NB100-638] - Knockdown of CAPER induces DSB proteins ATM & H2AX & leads to apoptosis in MDA-MB-231 cells(A) H2AX phosphorylation on ser139 is significantly increased (upper right quadrant population) in MDA-MB-231 cells after CAPER knockdown as represented by Muse Cell Analyzer plots (3-fold, p < 0.001, n = 3). (B) The increase in gamma H2AX was also validated through western blot analysis (2.5-fold, p < 0.01, n = 3). ATM phosphorylation on serine1981 is significantly upregulated after knockdown of CAPER expression (5-fold, p < 0.05, n = 3). (C) CAPER knockdown resulted in an increased level of caspase-3/7 activation through decreasing live cells (1.2-fold, p < 0.001, n = 3), while increasing apoptotic (7.5-fold, p < 0.001, n = 3), apoptotic/dead (6.5-fold, p < 0.001, n = 3), & dead (3-fold, p < 0.05, n = 3) cell populations. Interestingly, MDA-MB-231 cells expressing CAPER knockdown displayed no significant changes in any of the phases of the cell cycle (p = NS, n = 8, for G1, S & G2/M phases) compared to CTL shRNA (Figure 7C right panels). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30100993), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-638_rabbit-polyclonal-histone-h2ax-antibody-3102024155370.jpg)
HAF008). A specific band was detected for Phospho-Histone H2AX (S139) at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using 
NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for 
![Chromatin Immunoprecipitation: Histone H3 Antibody (1B1-B2) [NBP2-36468] - Chromatin from one million formaldehyde cross-linked HeLa cells was precipitated using 2 ug of NBP2-36468 and 25 ul of magnetic IgG beads, using standard ChIP methods. A similar sample containing no antibody was included as a negative control. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin. Representative target genes from active, inactive and heterochromatic regions of the genome show amplification, indicative of the presence of Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-1B1-B2-Chromatin-Immunoprecipitation-NBP2-36468-img0003.jpg "Chromatin Immunoprecipitation: Histone H3 Antibody (1B1-B2) [NBP2-36468] - Chromatin from one million formaldehyde cross-linked HeLa cells was precipitated using 2 ug of NBP2-36468 and 25 ul of magnetic IgG beads, using standard ChIP methods. A similar sample containing no antibody was included as a negative control. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin. Representative target genes from active, inactive and heterochromatic regions of the genome show amplification, indicative of the presence of Histone H3.")
![Western Blot: Histone H3 Antibody (1B1-B2) [NBP2-36468] - Western blot image of Anti-Histone H3 antibody (clone 1B1-B2). Whole cell protein from HeLa (lane 1), NIH-3T3 (lane 2) and PC12 (lane 3) were separated by 4-15% SDS-PAGE and transfered to PVDF membrane. The membrane was probed with purified Anti-Histone H3 antibody at 2 ug/ml and detected with an HRP conjugated anti-mouse secondary using chemiluminescence. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-1B1-B2-Western-Blot-NBP2-36468-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody (1B1-B2) [NBP2-36468] - Analysis of Histone H3 in human breast cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-1B1-B2-Immunohistochemistry-Paraffin-NBP2-36468-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody (1B1-B2) [NBP2-36468] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Histone H3 (1B1-B2) NBP2-36468 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-1B1-B2-Immunocytochemistry-Immunofluorescence-NBP2-36468-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Histone H3 Antibody (1B1-B2) [NBP2-36468] - Analysis in Hela cells using siRNA to knockdown Pol II, controls are GAPDH and Histone H3. Theoretical molecular weight of Histone H3 is 15 kDa. Image from a verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-1B1-B2-Western-Blot-NBP2-36468-img0009.jpg)
![Flow Cytometry: Histone H3 Antibody (1B1-B2) - BSA Free [NBP2-36468] - Analysis using Alexa Fluor (R) 647 conjugate of NBP2-36468. Jurkat cells stained with Alexa Fluor 647 conjugated Histone H3 antibody (red) or isotype control (blue). Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/nbp2-36468_mouse-monoclonal-histone-h3-antibody-1b1-b2-17720241044623.jpg)
![Western Blot: Histone H2a Antibody [NB100-56346] - Histone H2A in MCF-7 and HCT-116 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H2a-Antibody-Western-Blot-NB100-56346-img0004.jpg "Western Blot: Histone H2a Antibody [NB100-56346] - Histone H2A in MCF-7 and HCT-116 cells. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Histone H2a Antibody [NB100-56346] - Human breast probed with Histone H2A antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Histone-H2a-Antibody-Immunohistochemistry-Paraffin-NB100-56346-img0006.jpg)
![Western Blot: Histone H2a Antibody [NB100-56346] - Analysis of Histone H2A in human PBMC lysate probed with Histone H2A antibody.](http://images.novusbio.com/fullsize/Histone-H2a-Antibody-Western-Blot-NB100-56346-img0002.jpg)
![Western Blot: Histone H2AX Antibody [NB100-383] - Whole cell lysate (50 ug) from HEK293T cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-Western-Blot-NB100-383-img0043.jpg "Western Blot: Histone H2AX Antibody [NB100-383] - Whole cell lysate (50 ug) from HEK293T cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: Histone H2AX Antibody [NB100-383] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-H2AX (NB100-383). Detection: DAB](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-Immunohistochemistry-NB100-383-img0040.jpg)
![Western Blot: Histone H2AX Antibody [NB100-383] - Detection of mouse H2AX by western blot. Samples: Whole cell lysate (50 ug) from F9 and L1210 cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody A300-083A (lot A300-083A-5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-Simple-Western-NB100-383-img0039.jpg)
![Simple Western: Histone H2AX Antibody [NB100-383] - Simple Western lane view shows a specific band for H2AX in 0.2 mg/ml of HEK lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-Simple-Western-NB100-383-img0031.jpg)
![Simple Western: Histone H2AX Antibody [NB100-383] - Electropherogram image(s) of corresponding Simple Western lane view. H2AX antibody was used at 10 ug/ml dilution on HEK lysate(s).](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-Simple-Western-NB100-383-img0032.jpg)
![Western Blot: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - Whole extract of HeLa cell treated with nocodazole was separated on SDS-PAGE and probed with Monoclonal Anti-phospho-Histone H3 (pSer28), Clone: HTA28. The antibody was developed using Goat Anti-Rat IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. Antibody dilution 0.5 ug/mL2. Antibody dilution 1 ug/mL3. Antibody dilution 2 ug/mL](http://images.novusbio.com/fullsize/Histone-H3-p-Ser28-Antibody-HTA28-Western-Blot-NB600-1168-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - Whole extract of HeLa cell treated with nocodazole was separated on SDS-PAGE and probed with Monoclonal Anti-phospho-Histone H3 (pSer28), Clone: HTA28. The antibody was developed using Goat Anti-Rat IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. Antibody dilution 0.5 ug/mL2. Antibody dilution 1 ug/mL3. Antibody dilution 2 ug/mL")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 5 ug/mL Monoclonal Anti-phospho-Histone H3 (pSer28), Clone: HTA28. The antibody was developed using Goat Anti-Rat IgG, FITC conjugate (green). Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/Histone-H3-p-Ser28-Antibody-HTA28-Immunocytochemistry-Immunofluorescence-NB600-1168-img0007.jpg)
![Flow Cytometry: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - FACS profile of human leukemic cells with Anti-phospho-Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-p-Ser28-Antibody-HTA28-Flow-Cytometry-NB600-1168-img0006.jpg)
