| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56340SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1669SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22152SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-126-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-03993SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NB100-56343AFP488 | |||
| Alexa Fluor Plus 555 | NB100-56343AFP555 | |||
| Alexa Fluor Plus 594 | NB100-56343AFP594 | |||
| Alexa Fluor Plus 647 | NB100-56343AFP647 | |||
| Alexa Fluor Plus 680 | NB100-56343AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56343SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44032 | |||
| BSA Free | NBP2-52937-25ul | |||
![Western Blot: HDAC1 Antibody [NB100-56340] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HDAC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human HDAC1 Polyclonal Antibody (Catalog # NB100-56340) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 65 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Knockout-Validated-NB100-56340-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NB100-56340] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HDAC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human HDAC1 Polyclonal Antibody (Catalog # NB100-56340) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 65 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NB100-56340] - Analysis of HDAC1 in HEK293 cell lysate with this antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NB100-56340-img0002.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NB100-56340] - Human testis using HDAC1 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NB100-56340-img0005.jpg)
![Simple Western: HDAC1 Antibody [NB100-56340] - Simple Western lane view shows a specific band for HDAC1 in 0.5 mg/ml of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Simple-Western-NB100-56340-img0003.jpg)
![Western Blot: HDAC3 Antibody [NB100-1669] - Analysis of HDAC3 in HeLa cell lysate with anti-HDAC3 this antibody.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NB100-1669-img0002.jpg "Western Blot: HDAC3 Antibody [NB100-1669] - Analysis of HDAC3 in HeLa cell lysate with anti-HDAC3 this antibody.")
![Immunocytochemistry/Immunofluorescence: HDAC3 Antibody [NB100-1669] - HDAC3 preferably binds to nuclear Htt with long Qs. HDAC3 associates exclusively with nuclear inclusion bodies. E3 or N3 cells were fixed and stained with anti-HDAC3 antibodies and visualized by Alexa 546 conjugated secondary antibodies. Arrowheads: inclusion bodies with no HDAC3 signals associated. Arrows: HDAC3 signal-associated inclusion bodies. Bar=20 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111277), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1669-img0008.jpg)
![Immunohistochemistry-Paraffin: HDAC3 Antibody [NB100-1669] - Analysis human colon cancer using 1:50 conc. of HDAC3 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of HDAC3 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Immunohistochemistry-Paraffin-NB100-1669-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC3 Antibody [NB100-1669] - HDAC3 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue). An antibody dilution of 1:10 was used. Image objective 40x.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1669-img0004.jpg)
![Simple Western: HDAC3 Antibody [NB100-1669] - Lane view shows a specific band for HDAC3 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Simple-Western-NB100-1669-img0005.jpg)
![Western Blot: HDAC5 Antibody [NBP2-22152] - LNCaP cells with normal medium and with androgen depletion. Image from verified customer review.](http://images.novusbio.com/fullsize/HDAC5-Antibody-Western-Blot-NBP2-22152-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: HDAC5 Antibody [NBP2-22152] - LNCaP cells with normal medium and with androgen depletion. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HDAC5 Antibody [NBP2-22152] - HDAC5 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/HDAC5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22152-img0001.jpg)
![Immunohistochemistry: HDAC5 Antibody [NBP2-22152] - IHC analysis of HDAC5 in mouse pancreas.](http://images.novusbio.com/fullsize/HDAC5-Antibody-Immunohistochemistry-NBP2-22152-img0002.jpg)
![Western Blot: HDAC5 Antibody [NBP2-22152] - WB analysis of HDAC5 in 1. HeLa, 2. Ntera 2, 3. HepG2 and 4. MCF7 cell lysates.](http://images.novusbio.com/fullsize/HDAC5-Antibody-Western-Blot-NBP2-22152-img0003.jpg)
![Western Blot: HDAC3 Antibody - BSA Free [NB500-126] - Cell line lysates were separated on SDS-PAGE and probed with 1:5,000 Rabbit Anti-HDAC3. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. HEK-293T; 2. HeLa; 3. K562; 4. COS-7; 5. CHO; 6. NIH-3T3; 7. Neuro-2a; 8. PC-12; 9. NRK](http://images.novusbio.com/fullsize/HDAC3-Antibody---BSA-Free-Western-Blot-NB500-126-img0006.jpg "Western Blot: HDAC3 Antibody - BSA Free [NB500-126] - Cell line lysates were separated on SDS-PAGE and probed with 1:5,000 Rabbit Anti-HDAC3. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. HEK-293T; 2. HeLa; 3. K562; 4. COS-7; 5. CHO; 6. NIH-3T3; 7. Neuro-2a; 8. PC-12; 9. NRK")
![Immunocytochemistry/Immunofluorescence: HDAC3 Antibody - BSA Free [NB500-126] - HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 1:200 Rabbit Anti-HDAC3. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.](http://images.novusbio.com/fullsize/HDAC3-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB500-126-img0004.jpg)
![Immunohistochemistry: HDAC3 Antibody - BSA Free [NB500-126] - Formalin-fixed, paraffin-embedded section of lymph node stained with 1:500 Anti-Histone Deacetylase 3 (HDAC3) antibody produced in Rabbit followed by Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat and by ExtrAvidin(R)-Peroxidase](http://images.novusbio.com/fullsize/HDAC3-Antibody---BSA-Free-Immunohistochemistry-NB500-126-img0005.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0029.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Rat brain. Green: HDAC1 antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0024.jpg)
![Chromatin Immunoprecipitation: HDAC1 Antibody [NBP1-31294] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of HDAC1 antibody The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Chromatin-Immunoprecipitation-NBP1-31294-img0004.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Sample: 1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of HDAC1 protein by 2. 5 ug of HDAC1 antibody 10% SDS-PAGE gel.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0012.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-31294] - Analysis of A431, using HDAC1 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31294-img0003.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Sample: Paraffin-embedded mouse liver. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0019.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Rat brain. HDAC1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0020.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Mouse liver. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0021.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Mouse colon. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0022.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-91020242041272.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420134061.jpg)
![Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody [NBP1-31294] - HDAC1 antibody detects HDAC1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HDAC1 stained by HDAC1 antibody (NBP1-31294) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420131216.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-31294] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using HDAC1 antibody (NBP1-31294) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-91020242001098.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Sample (30 ug of whole cell lysate) A: zebrafish eye 10% SDS PAGE NBP1-31294 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420115068.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-31294] - HDAC1 antibody detects Hdac1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. HDAC1 antibody (NBP1-31294) dilution: 1:100.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420115022.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-91020242021910.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC11 Antibody [NBP2-16789] - Sox2 gene expression is regulated by Gli1 and HDAC11. Double immunofuorescence assay with Gli1 and HDAC11 showed co-localization of both in A549 (upper panel) and H1650 (lower panel) cells. DAPI was used to stain the nucleus of the cells. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-020-61295-6) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HDAC11-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16789-img0009.jpg "Immunocytochemistry/Immunofluorescence: HDAC11 Antibody [NBP2-16789] - Sox2 gene expression is regulated by Gli1 and HDAC11. Double immunofuorescence assay with Gli1 and HDAC11 showed co-localization of both in A549 (upper panel) and H1650 (lower panel) cells. DAPI was used to stain the nucleus of the cells. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-020-61295-6) licensed under a CC-BY license.")
![Immunohistochemistry: HDAC11 Antibody [NBP2-16789] - HDAC11 expression in human lung tumor tissues and cells and its correlation with patient prognosis. Elevated HDAC11 staining is seen in NSCLC tissue and its metastatic sites as compared to the normal human lung tissue in TMA. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-020-61295-6) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HDAC11-Antibody-Immunohistochemistry-NBP2-16789-img0010.jpg)
![Simple Western: HDAC11 Antibody [NBP2-16789] - 1:25 dilution against the indicated concentration of protein lysate of human SVG-A cells. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HDAC11-Antibody-Simple-Western-NBP2-16789-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC11 Antibody [NBP2-16789] - HDAC11 silencing decreases expression of downstream targets and abrogates self-renewal of CSCs. Depletion of HDAC11 using two IPTG inducible shRNA shows a significant decrease in Sox2, YAP1 and Gli1 protein expression as compared to IPTG treated control shRNA. The glycolysis pathway targets HK2 and PDK2 also show reduced protein expression in the absence of HDAC11. No change is observed in PDK1 expression; the full images of the western blots are provided as Supplementary Fig. 4. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-020-61295-6) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HDAC11-Antibody-Knockdown-Validated-NBP2-16789-img0011.jpg)
![Western Blot: HDAC11 Antibody [NBP2-16789] - HDAC11 silencing decreases expression of downstream targets & abrogates self-renewal of CSCs. (A) Depletion of HDAC11 using two IPTG inducible shRNA shows a significant decrease in Sox2, YAP1 & Gli1 protein expression as compared to IPTG treated control shRNA. The glycolysis pathway targets HK2 & PDK2 also show reduced protein expression in the absence of HDAC11. No change is observed in PDK1 expression; the full images of the western blots are provided as Supplementary Fig. 4. (B) Quantitation of the western blot band intensities using ImageJ analysis. (C) Sphere formation assay with SP cells from shHDAC11 H1650 cells treated with IPTG show abrogation of self-renewal ability in the absence of HDAC11. (D) Quantitation of sphere formation assay reveal that IPTG treated SP cells from two different clones of HDAC11 shRNA containing H1650 form fewer spheres as compared to IPTG untreated or control shRNA containing cells. (E,F) Real time PCR analysis of H1650 cells with two different HDAC11 shRNA show a decrease in mRNA expression of Sox2, HK2, PDK1, PDK2 with IPTG treatment. No significant change was observed in Oct4 & Nanog expression. The depletion of HDAC11 was confirmed using RT-PCR. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32170113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16789_rabbit-polyclonal-hdac11-antibody-310202416114794.jpg)
![Western Blot: HDAC9 Antibody [NBP2-03993] - Analysis of HDAC-9 in human HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) and mouse RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 0.5 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HDAC9-Antibody-Western-Blot-NBP2-03993-img0004.jpg "Western Blot: HDAC9 Antibody [NBP2-03993] - Analysis of HDAC-9 in human HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) and mouse RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 0.5 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: HDAC6 Antibody [NB100-56343] - Analysis of HDAC-6 in NIH-3T3 cell lysate with this antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NB100-56343-img0002.jpg "Western Blot: HDAC6 Antibody [NB100-56343] - Analysis of HDAC-6 in NIH-3T3 cell lysate with this antibody.")
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NB100-56343] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-HDAC6 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56343-img0004.jpg)
![Simple Western: HDAC6 Antibody [NB100-56343] - Lane view shows a specific band for HDAC6 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Simple-Western-NB100-56343-img0003.jpg)
![Immunohistochemistry-Paraffin: HDAC3 Antibody [NBP1-31196] - SW480 xenograft, using HDAC3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Immunohistochemistry-Paraffin-NBP1-31196-img0008.jpg "Immunohistochemistry-Paraffin: HDAC3 Antibody [NBP1-31196] - SW480 xenograft, using HDAC3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Chromatin Immunoprecipitation: HDAC3 Antibody [NBP1-31196] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of HDAC3 antibody The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Chromatin-Immunoprecipitation-NBP1-31196-img0006.jpg)
![Western Blot: HDAC3 Antibody [NBP1-31196] - Analysis of on: A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 10% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP1-31196-img0009.jpg)
![Western Blot: HDAC3 Antibody [NBP1-31196] - Sample: 1000 ug 293T whole cell lysate/extract A. 50 ug 293T whole cell lysate/extract, B. Control with 2 ug of preimmune rabbit IgG, C. Immunoprecipitation of HDAC3 protein by 2 ug of HDAC3 antibody 10% SDS-PAGE gel.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP1-31196-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC3 Antibody [NBP1-31196] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP1-31196-img0011.jpg)
![Western Blot: HDAC3 Antibody [NBP1-31196] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP1-31196-img0019.jpg)
![Western Blot: HDAC3 Antibody [NBP1-31196] -Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-31196_rabbit-polyclonal-hdac3-antibody-western-blot-168202492658..jpg)
![Western Blot: HDAC3 Antibody [NBP1-31196] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-31196_rabbit-polyclonal-hdac3-antibody-western-blot-168202492824..jpg)
![Western Blot: HDAC3 Antibody [NBP1-31196] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term (NBP1-31196) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31196_rabbit-polyclonal-hdac3-antibody-910202420122079.jpg)
![HDAC3 antibody [C3], C-term detects HDAC3 protein at nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HDAC3 protein stained by HDAC3 antibody [C3], C-term (NBP1-31196) diluted at 1:400.Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-31196_rabbit-polyclonal-hdac3-antibody-immunocytochemistry-immunofluorescence-141120251838416.jpg)
![HDAC3 antibody [C3], C-term detects HDAC3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HDAC3 protein stained by HDAC3 antibody [C3], C-term (NBP1-31196) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-31196_rabbit-polyclonal-hdac3-antibody-immunocytochemistry-immunofluorescence-1411202518373815.jpg)
![Western Blot: HDAC3 Antibody [NBP2-16791] - HDAC3 antibody detects HDAC3 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP2-16791-img0015.jpg "Western Blot: HDAC3 Antibody [NBP2-16791] - HDAC3 antibody detects HDAC3 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted by 1:1000.")
![Immunohistochemistry-Paraffin: HDAC3 Antibody [NBP2-16791] - Immunohistochemical analysis of paraffin-embedded OVCA, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Immunohistochemistry-Paraffin-NBP2-16791-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC3 Antibody [NBP2-16791] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP2-16791-img0004.jpg)
![Western Blot: HDAC3 Antibody [NBP2-16791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP2-16791-img0014.jpg)
![HDAC3 antibody detects HDAC3 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HDAC3 protein stained by HDAC3 antibody (NBP2-16791) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-16791_rabbit-polyclonal-hdac3-antibody-immunocytochemistry-immunofluorescence-261120251962718.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M14), clone 5C11. Lane 1: HDAC1 transfected lysatE (55.1 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0016.jpg "Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M14), clone 5C11. Lane 1: HDAC1 transfected lysatE (55.1 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of monoclonal antibody to HDAC1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Immunocytochemistry-Immunofluorescence-H00003065-M14-img0009.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Immunohistochemistry-Paraffin-H00003065-M14-img0010.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0012.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0013.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0014.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0015.jpg)
![ELISA: HDAC1 Antibody (5C11) [H00003065-M14] - Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-ELISA-H00003065-M14-img0008.jpg)
![Proximity Ligation Assay: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of protein-protein interactions between NFKB1 and HDAC1. HeLa cells were stained with anti-NFKB1 rabbit purified polyclonal 1:1200 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Proximity-Ligation-Assay-H00003065-M14-img0011.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Analysis in human rectum and skeletal muscle tissues using NBP2-52937 antibody. Corresponding HDAC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Immunohistochemistry-Paraffin-NBP2-52937-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Analysis in human rectum and skeletal muscle tissues using NBP2-52937 antibody. Corresponding HDAC1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: HDAC1 Antibody (CL0510) [NBP2-52937] - analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HDAC1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Western-Blot-NBP2-52937-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of U-251 cells using the Anti-HDAC1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Immunocytochemistry-Immunofluorescence-NBP2-52937-img0010.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Immunohistochemistry-Paraffin-NBP2-52937-img0015.jpg)
![Western Blot: HDAC1 Antibody (CL0510) [NBP2-52937] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Western-Blot-NBP2-52937-img0008.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human rectum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Immunohistochemistry-Paraffin-NBP2-52937-img0012.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Immunohistochemistry-Paraffin-NBP2-52937-img0013.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Immunohistochemistry-Paraffin-NBP2-52937-img0014.jpg)