Product Search for CD7: Novus Biologicals

AF1062

PDGFR alpha Antibody [Unconjugated]

(4 Reviews)

100 μg

AF1062

HAF017). Specific bands were detected for PDGF Ra at approximately 160-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse uterus tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). Specific bands were detected for PDGF Ra at approximately 160-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse uterus tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for PDGF Ra at approximately 160-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

PDGF Ra was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of mesenchymal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

PDGF Ra was detected in immersion fixed frozen sections of mouse embryo using 15 µg/mL Goat Anti-Mouse PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of mesenchymal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

221-AA) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (AR002. Proliferation elicited by Recombinant Human PDGF-AA (250 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062). The ND₅₀ is typically 0.2-1.6 µg/mL." class="big_lightbox">

221-AA) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (AR002. Proliferation elicited by Recombinant Human PDGF-AA (250 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062). The ND₅₀ is typically 0.2-1.6 µg/mL." class="big_thumb" />

Extracellularly applied recombinant human a-syn PFFs induced cytoplasmic a-syn-immunoreactive inclusions in primary BCAS1(+) cell cultures. Immunostaining of oligodendroglial cells incubated with 1?µM a-syn PFFs for 24?h from days 3 (upper) and 4 (lower) after differentiation induction showing the ubiquitous development of thioflavin S-labeled inclusions in PDGFRa(+) cells and BCAS1(+) cells. In contrast, few BCAS1(-)/MBP(+) cells developed thioflavin S-labeled inclusions. Scale bar?=?50?µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32727582), licensed under a CC-BY licence.

Rspo3 mRNAs are localized on telopodes that extend away from the cell bodies of the mouse VTTs. VTTs are marked by Lgr5 mRNA (red dots), Rspo3 mRNA (green dots) is localized away from the cell body, PDGFRa antibody mark VTTs cell bodies and telopodes. Scale bar–10?µm, in inset, green arrows point to Rspo3 mRNAs (green dots) localized on PDGFRa telopodes (blue). Telocyte cell body is marked by white dashed line. inset Scale bar–5?µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32321913), licensed under a CC-BY licence.

OPC expresses leptin receptors. Representative images of mouse spinal cord sections, which were double-labeled for LepRb (green) in combination with PDGFRa (red). Spinal cord sections were obtained 7 days after LPC injection; the graph shows quantification (n?=?3). P?=?0.007573 (LepRb flox vs intact CKO), 0.0108779 (LepRb flox vs LPC CKO), ANOVA with Tukey’s post-hoc test. Scale bar: 25?µm. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep40397), licensed under a CC-BY licence.

LPC injection does not enhance leptin expression in the CNS. Representative images of LepRb (green) expression in combination with PDGFRa, GFAP NeuN, and CD11b (red) in the mouse spinal cord with or without LPC injection. Spinal cord sections were obtained 3 days after LPC injection. Graph indicates the relative intensity of leptin protein expression in indicated cell type (n?=?3). P?=?0.287452 (PDGFRa), 0.181059 (GFAP), 0.199972 (NeuN), Student’s t-test, n.s. indicates no significant difference. *P?<?0.05, **P?<?0.01, error bars represent SEM. Scale bar: 25?µm. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep40397), licensed under a CC-BY licence.

PDGFRa driven mouse brain tumor model. Example of early stage tumor growth, as revealed by IHC for proliferation marker Ki67 and PDGFRa. Note high density of Ki67+ proliferating cells in tumor area, increased expression level of PDGFRa, and invasive migration of tumor cells through corpus callosum into contralateral hemisphere. Scale bar: 50 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25683249), licensed under a CC-BY licence.

af1062_mouse-pdgf-r-alpha-affinity-purified-polyclonal-ab-212024165018.jpg

Endogenous leptin sustains spontaneous OPC proliferation. Representative images of mouse spinal cord sections, which were prepared 7 days (left panels) and 14 days (right panels) after LPC injection and double labeled for BrdU in combination with PDGFRa (upper panels), GSTp (upper panels) and olig2 (lower panels). BrdU was administrated during 3–7 days after LPC injection; the graph shows quantification (n?=?5–8). P?=?0.042915 (PDGFRa and BrdU labeled cells), 0.013560 (Olig2 and BrdU labeled cells 7 days after injection), 0.012111 (GSTp and BrdU labeled cells), 0.009797 (Olig2 and BrdU labeled cells 14 days after injection), Student’s t-test. Scale bar: 50?µm. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep40397), licensed under a CC-BY licence.

Etv5 is necessary and sufficient for proliferation and cell fate bias downstream of Cic loss. Cic-null mouse NSCs (CicnullEmpty), Cic-null mouse NSCs with dominant negative Etv5 (CicnullDN-Etv5), and Cic-wildtype mouse NSCs overexpressing Etv5 (Etv5 overpression) were grown in lineage-directed culture conditions and assessed for their ability to differentiate to oligodendrocytes as determined by immunostaining for Olig2, Pdgfra, and Mbp. Scale bar: 10?µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31043608), licensed under a CC-BY licence.

Transplantation of CD11b/LIF transgenic BMCs reduces the numbers of FAPs in dystrophic muscle but does not affect phenotype. To quantify the number of FAPs, transgenic mouse muscle sections were co-labeled with antibodies to PDGFRa (red) and CD31, CD45 (green). Arrowheads indicate FAPs (CD31-CD45-PDGFRa+). Bar?=?50?µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31243277), licensed under a CC-BY licence.

Lgr5 is expressed abundantly in mouse villus tip telocytes. d) Lgr5 mRNA (red dots) expressed in PDGFRa+ VTTs that co-express Bmp4 mRNA (green dots). Scale bar–10?µm. Red arrows point to Lgr5 and Bmp4 double positive cells. e) Blow up of the region boxed in d). Scale bar–5?µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32321913), licensed under a CC-BY licence.

PDGFR alpha driven brain tumors display features of high grade glioma.(a–g) Histopathological analysis of tumor areas by H&E staining shows a high concentration of mitotic figures (a, arrows), high cellularity and nuclear atypia (b), perineuronal satellitosis (c; N, neuronal nuclei), perivascular growth (d), intrafascicular growth (e), subarachnoid spreading (f), and areas of incipient necrosis (g; arrows point to pyknotic nuclei). (h–k) IF labeling of brain tumor sections for cell type specific markers. Nuclei labeled with DAPI are shown in blue. Tumor cells with high PDGFR alpha expression were highly proliferative, as seen by proliferation marker Ki67 (h), and express the OPC cell lineage markers Olig2, Sox2, Sox10, and Ng2, as well as the neural stem cell marker Nestin (i–k). Tumor cells were negative for immunosignal of astroglial marker GFAP, mature oligodendrocyte marker APC-CC1, and neuronal marker NeuN (l–n). Scale bars: 10 μm (a–g), 20 μm (h–n). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25683249), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Genetic Strategies Validation. </b>PDGFR alpha driven brain tumor model.(a) Schematic diagram of PDGFR alpha J/K knock-in alleles. ATG, start codon; SA, splice acceptor; STOP, PGK-neo cassette. (b) Kaplan-Meier survival curves of 4 mouse mutant cohorts with brain tumors. Mice generally succumbed to subcutaneous fibrosarcomas, and brain tumors were detected by histological analysis. (c) Example of early stage tumor growth, as revealed by IHC for proliferation marker Ki67 and PDGFR alpha . Note high density of Ki67+ proliferating cells in tumor area, increased expression level of PDGFR alpha , and invasive migration of tumor cells through corpus callosum into contralateral hemisphere. (d) H&E staining of an advanced brain tumor growth (asterisk in tumor centre, dashed line demarcates expansion). Scale bars: 50 μm (c, d). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25683249), licensed under a CC-BY license. Not internally tested by R&D Systems.

LPC injection does not enhance leptin expression in the CNS.(a) Representative images of MBP expression in a mouse spinal cord 14 days after LPC injection are shown; the graph shows quantification of the demyelinating area in the dorsal spinal cord (n = 3–4). P = 0.001542, Student’s t-test. (b) Representative images of NeuN expression in a mouse spinal cord 14 days after LPC injection; the graph shows quantification of the density of NeuN-positive cells in the spinal cord (n = 3). P = 0.299940, Student’s t-test, n.s. indicates no significant difference. (c) Quantification of leptin protein expression in indicated organs. Tissues were obtained from the mice 3 days after LPC injection (n = 3 for control, 4 for LPC injection). P = 0.318966 (adipose tissue), 0.10446 (brain stem), 0.332281 (cerebellum), 0.345245 (liver), 0.453104 (kidney), 0.098135 (heart), 0.335722 (lung), 0.236771 (muscle), 0.44662 (spleen), 0.465966 (stomach). Student’s t-test. n.s. indicates no significant difference. (d) Quantification of spinal cord leptin protein 3 days after LPC injection (n = 3 for control, 4 for LPC injection). P = 0.026865, Student’s t-test. (e) Quantification of spinal cord leptin mRNA 3 days after LPC injection (n = 6). P = 0.324930, Student’s t-test, n.s. indicates no significant difference. (f) Representative images of LepRb (green) expression in combination with PDGFR alpha , GFAP NeuN, and CD11b (red) in the mouse spinal cord with or without LPC injection. Spinal cord sections were obtained 3 days after LPC injection. Graph indicates the relative intensity of leptin protein expression in indicated cell type (n = 3). P = 0.287452 (PDGFR alpha ), 0.181059 (GFAP), 0.199972 (NeuN), Student’s t-test, n.s. indicates no significant difference. *P < 0.05, **P < 0.01, error bars represent SEM. Scale bars; 100 μm for (a and b), 25 μm for (f). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep40397), licensed under a CC-BY license. Not in

<b>Biological Strategies Validation. </b>Leptin promotes OPC proliferation.(a) Representative image of cultured OPCs stained with antibodies against LepRb (green) and PDGFR alpha (red). Scale bar: 25 μm. (b) Relative BrdU incorporation into the OPC obtained from the brain (left graph) and spinal cord (right graph). Cells were treated with recombinant leptin for 48 h (n = 4). (Left graph) P = 0.005993 (control vs 10 ng/mL), 0.045616 (control vs 100 ng/mL), (Right graph) P = 0.004456 (control vs 10 ng/mL), 0.017859 (control vs 100 ng/mL). (c) Relative BrdU incorporation into the OPC after leptin stimulation (10 ng/ml) with U0126 (20 μM), a MEK inhibitor (n = 4 for brain OPCs, n = 3 for spinal cord OPCs). (Left graph) P = 0.019753 (control vs leptin), 0.039433 (leptin vs leptin + U0126), (Right graph) P = 0.045545 (control vs leptin), 0.04486 (leptin vs leptin + U0126). (d) Representative images of western blotting (upper panels) and quantitative analysis of ERK phosphorylation (lower graph) are shown. OPCs were treated with leptin (10 ng/ml) under indicated periods (n = 3). P = 0.006352 (2 min), 0.016571 (5 min), 0.017675 (10 min), 0.024100 (15 min), 0.081342 (30 min). *P < 0.05, **P < 0.01, ANOVA with Tukey’s post-hoc test. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep40397), licensed under a CC-BY license. Not internally tested by R&D Systems.

Extracellularly applied recombinant human alpha -syn PFFs induced cytoplasmic alpha -syn-immunoreactive inclusions in primary BCAS1(+) cell cultures. a Confocal images of BCAS1(+) cells, which were incubated with 1 μM alpha -syn PFFs for 24 h from day 4 after differentiation induction, showing the intracellular inclusions labeled with both anti-alpha -syn antibody and thioflavin S. Scale bar = 5 μm. b Immunostaining of oligodendroglial cells incubated with 1 μM alpha -syn PFFs for 24 h from days 3 (upper) and 4 (lower) after differentiation induction showing the ubiquitous development of thioflavin S-labeled inclusions in PDGFR alpha (+) cells and BCAS1(+) cells. In contrast, few BCAS1(−)/MBP(+) cells developed thioflavin S-labeled inclusions. Scale bar = 50 μm. c The percentages of oligodendroglial cells containing thioflavin S-labeled inclusions were compared between BCAS1(−)/PDGFR alpha (+) cells and BCAS1(+)/PDGFR alpha (+) cells (upper, performed on day 3), and between BCAS1(+)/MBP(+) cells and BCAS1(−)/MBP(+) cells (lower, performed on day 4). N = 4, respectively, independent culture, Mann–Whitney, p* < 0.05 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32727582), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Transplantation of CD11b/LIF transgenic BMCs reduces the numbers of FAPs in dystrophic muscle but does not affect phenotype. a QPCR analysis shows that TA muscles from LIF BMT/mdx recipients have reduced Pdgfra gene expression. N = 7 or 8 for WT BMT/mdx and LIF BMT/mdx data sets, respectively, * indicates significantly different from WT BMT/mdx recipients at P < 0.05. P-values based on two-tailed t-test. For all histograms in the figure, the bars indicate mean ± sem. b To quantify the number of FAPs, muscle sections were co-labeled with antibodies to PDGFR alpha (red) and CD31, CD45 (green). Arrowheads indicate FAPs (CD31-CD45-PDGFR alpha +). Bar = 50 μm. c Fewer FAPs (CD31-CD45-PDGFR alpha +) in TA cross-sections of LIF BMT/mdx recipients compared to WT BMT/mdx recipients. N = 5 for each data set. d There was no detectible change in phenotype of PDGFR alpha + cells assayed for co-expression of the fibrogenic marker HSP47. e FACS plots demonstrating strategy for sorting FAPs (Hoechst + CD11b-CD31-CD45-PDGFR alpha +). Fibroblasts derived from FAPs were stimulated with LIF (10 ng/ml) and/or TGF beta 1 (10 ng/ml) for 3 h (f–h) or 3 days (i–k) and assayed by QPCR for Ctgf (f, i), Fn1 (g, j), and Col1a1 (h, k). N = 4 technical replicates for each data set. Significant findings were verified with biological replicates of cells sorted from independent donors. * Indicates significantly different from control cultures, # indicates significantly different from TGF beta 1 treated cultures, and Φ indicates significantly different from LIF-treated cultures at P < 0.05. P-values based on ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31243277), licensed under a CC-BY license. Not internally tested by R&D Systems.

Lgr5 is expressed abundantly in villus tip telocytes.a smFISH of Lgr5, DAPI in blue, Scale bar–20 µm. b Blow up of villus tip, Scale bar–10 µm. In a, b thin white arrows point at autofluorescent blobs. c blow up of crypt, Scale bar–10 µm. Red arrows in b–c point to Lgr5 positive cells. dLgr5 mRNA (red dots) expressed in PDGFRa+ VTTs that co-express Bmp4 mRNA (green dots). Scale bar–10 µm. Red arrows point to Lgr5 and Bmp4 double positive cells. e Blow up of the region boxed in d. Scale bar–5 µm. fLgr5 mRNA concentrations in VTTs are comparable to those in Lgr5+ crypt base columnar cells (n = 25 cells examined over 2 mice for each region). Boxes show 25–75 percentiles of the smFISH expression, horizontal red lines are medians. Whiskers, extend to the most extreme data point within 1.5× the interquartile range (IQR) from the box; g) Rspo3 mRNAs are localized on telopodes that extend away from the cell bodies of the VTTs. VTTs are marked by Lgr5 mRNA (red dots), Rspo3 mRNA (green dots) is localized away from the cell body, PDGFRa antibody mark VTTs cell bodies and telopodes. Scale bar–10 µm, in inset, green arrows point to Rspo3 mRNAs (green dots) localized on PDGFRa telopodes (blue). Telocyte cell body is marked by white dashed line. inset Scale bar–5 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32321913), licensed under a CC-BY license. Not internally tested by R&D Systems.

OPC expresses leptin receptors.(a) Representative images of spinal cord sections, which were double-labeled for LepRb (green) in combination with PDGFR alpha (red). Spinal cord sections were obtained 7 days after LPC injection; the graph shows quantification (n = 3). P = 0.007573 (LepRb flox vs intact CKO), 0.0108779 (LepRb flox vs LPC CKO), ANOVA with Tukey’s post-hoc test. (b) Relative expression of leptin receptors mRNA in PDGFR alpha -positive OPC obtained from the brain of PDGFR alpha -creERT:: Lepr flox/flox mice and +/+::Lepr flox/flox mice (n = 5,6). P = 0.005878 (LepRa), 0.010306 (LepRb), 0.001535 (LepRc), 0.003169 (LepRd), 0.030459 (LepRe), Student’s t-test. (c) Representative images of spinal cord sections which were double labeled for BrdU in combination with PDGFR alpha (left panels) and GSTπ (right panels). Sections were prepared 7 days (left panels) and 14 days (right panels) after LPC injection. BrdU was administrated during 3–7 days after LPC injection; the graph shows quantification (n = 5–8). P = 0.029791(PDGFR alpha and BrdU labeled cells), 0.028870 (GSTπ and BrdU labeled cells), Student’s t-test. (d) Representative images of PDGFR alpha expression in the intact spinal cord of PDGFR alpha -creERT:: Lepr flox/flox mice and +/+::Lepr flox/flox mice; the graph shows quantification (n = 3–4). P = 0.404999, Student’s t-test, n.s. indicates no significant difference. (e) Representative images of APC expression in the intact spinal cord of PDGFR alpha -creERT:: Lepr flox/flox mice and +/+::Lepr flox/flox mice; the graph shows quantification (n = 3). P = 0.495667, Student’s t-test, n.s. indicates no significant difference. (f) Representative spinal cord section of PDGFR alpha -creERT:: Lepr flox/flox mice, which were prepared 14 days after LPC injection and stained with MBP; the graph shows quantification of the demyelinating area in the dorsal spinal cord (n = 7 for control, 10 for CKO). P = 0.030688, Student’s t-test. (g) Representative spinal cord sections which were labeled for CD11b. Sections were

Endogenous leptin sustains spontaneous OPC proliferation.(a) Representative images of spinal cord sections, which were prepared 7 days (left panels) and 14 days (right panels) after LPC injection and double labeled for BrdU in combination with PDGFR alpha (upper panels), GSTπ (upper panels) and olig2 (lower panels). BrdU was administrated during 3–7 days after LPC injection; the graph shows quantification (n = 5–8). P = 0.042915 (PDGFR alpha and BrdU labeled cells), 0.013560 (Olig2 and BrdU labeled cells 7 days after injection), 0.012111 (GSTπ and BrdU labeled cells), 0.009797 (Olig2 and BrdU labeled cells 14 days after injection), Student’s t-test. (b) Representative spinal cord sections, which were prepared 14 days after LPC injection and stained with MBP are shown; the graph shows quantification (n = 6). P = 0.025243, Student’s t-test. (c) Representative images of spinal cord section, which were prepared 7 days after LPC injection and labeled for CD11b; the graph shows quantification (n = 4). P = 0.213763, Student’s t-test, n.s. indicates no significant difference. *P < 0.05, **P < 0.01, error bars represent SEM. Scale bar: 50 μm for high magnification images in a, 100 μm for others. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep40397), licensed under a CC-BY license. Not internally tested by R&D Systems.

F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

3T3-L1 cells were stained with Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062, filled histogram) or isotype control antibody (Catalog # 4-001-A, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

PDGFR alpha and ciliary axonemes early in development. (A) High-resolution microscopy and (B,C) 3D IHC reconstructions of 15 µm wildtype tissue show phosphorylated PDGFR alpha (red) present along ciliary axonemes (acetylated tubulin, green) (arrowheads highlighted in C) at E13. By P0, the receptor localizes to the endocardium where cilia are absent (arrows). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33805717), licensed under a CC-BY license. Not internally tested by R&D Systems.

Impact of LPC surgery on mice subjected to running wheel. (A) Workflow and representative eriochrome cyanine-stained sections with lesion in the ventrolateral white matter delineated by dashed lines. Figure was drawn using BioRender. (B) Representative images of LPC lesion (left) and contralateral normal appearing white matter (right) 4 days post injury stained for mature oligodendrocytes (CC1) in red, OPCs (PDGFR alpha ) in green, oligodendrocyte lineage cells (OLIG2) in white, and DNA (DAPI) in blue. (C) Representative images of LPC lesion 4 days post injury stained for myelin and myelin debris (MBP) in red, axons (NFH) in green, and astrocytes (GFAP) in white, and DNA (DAPI) in blue. In both (B) and (C), the lesion is outlined by the irregular dashed line while the non-involved contralateral site is denoted by the rectangle dashed line. Scale bar represents 100 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33790323), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reduced number of MSCs at the periodontitis site in aged mice. Immunofluorescence images show the number of platelet-derived growth factor receptor alpha (PDGFR alpha )+ MSCs (red) in the furcation area in young and aged mice. Cell nuclei were stained with DAPI (blue). Bar: 100 µm. The graph shows the quantitative analysis indicating that the number of PDGFR alpha + Mesenchymal stem cells (MSCs) is decreased in aged mice, more prominently at day-10 after ligation. The bar graph represents the mean ± standard deviation of at least three independent samples. * p < 0.05, ** p < 0.01, *** p < 0.001, two-way ANOVA, Tukey test (n = 3). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33143068), licensed under a CC-BY license. Not internally tested by R&D Systems.

Nuclear entry of SIRT2 in OPCs during remyelination is impaired in the aged mice in vivo.a Primary culture of various types of cells from P0 rat cortex. b qRT-PCR of seven members of sirtuins in primary cultured OPCs and mature oligodendrocytes from the cortex of P0 rat (n = 3). c qRT-PCR of sirt2 in primary cultured various types of cells from the cortex of P0 rat (n = 3). d–f Immunofluorescence and quantification of SIRT2+ cells in the cortex of mice at different ages (n = 3). Scale bar, 10 μm (d), 50 μm (upper panel images of f), 5 μm (lower panel images of f). g–h Immunofluorescence of SIRT2 in the cortex of marmosets at postnatal day 3 (P3, g) or age of 8 years (h). Scale bar, 50 μm. i Immunohistochemistry of SIRT2 in the cortex of human at age of 53 years. Scale bar, 20 μm. j, k Relative SIRT2 protein level in brains of WT young (6 M) and old (18 M) mice (n = 3). l–o Immunofluorescence and quantification of SIRT2+ OPCs and nuclear SIRT2+ OPCs in corpus callosum of WT young and old mice (n = 3). NL, non-lesion, L, demyelination lesion induced by LPC at 5 dpl. Scale bar, 10 μm. All data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 by two-tailed t-test (k) or one-way ANOVA followed by Tukey’s post hoc test (m–o). In all instances ***p < 0.001. n.s. no significance. In (k), **p = 0.004; in (m), **p = 0.002 (L-Young vs. L-Old); in (n), **p = 0.007 (L-Young vs. L-Old). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35264567), licensed under a CC-BY license. Not internally tested by R&D Systems.

Primary goat anti-mouse PDGFRa (1:300) with Donkey anti-goat AF647 (1:500) on adult mouse pancreas. Image from a verified customer review.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1062U-100UG
Alexa Fluor 405	AF1062V-100UG
Alexa Fluor 488	FAB1062G
Alexa Fluor 532	AF1062X-100UG
Alexa Fluor 594	AF1062T-100UG
Alexa Fluor 647	AF1062R-100UG
Alexa Fluor 700	AF1062N-100UG
Alexa Fluor 750	AF1062S-100UG
Alexa Fluor Plus 405	AF1062AFP405-100UG
Alexa Fluor Plus 488	AF1062AFP488-100UG
Alexa Fluor Plus 555	AF1062AFP555-100UG
Alexa Fluor Plus 594	AF1062AFP594-100UG
Alexa Fluor Plus 647	AF1062AFP647-100UG
Alexa Fluor Plus 680	AF1062AFP680-100UG
Allophycocyanin	FAB1062A
Biotin	BAF1062
CoraFluor 1	AF1062CL1
DyLight 350	AF1062D
DyLight 405	AF1062E
DyLight 488	AF1062K
DyLight 550	AF1062L
DyLight 594	AF1062M
DyLight 650	AF1062W
DyLight 680	AF1062Y
DyLight 755	AF1062Z
FITC	AF1062F
HRP	AF1062H
Janelia Fluor 525	AF1062JF525
Janelia Fluor 549	AF1062I
Janelia Fluor 646	AF1062J
Janelia Fluor 669	AF1062JF669
PerCP	AF1062C
Phycoerythrin	FAB1062P
mFluor Violet 450 SE	AF1062MFV450
mFluor Violet 500 SE	AF1062MFV500
mFluor Violet 610 SE	AF1062MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB1062P
Carrier Free	FAB1062A
Carrier Free	FAB1062G
Carrier Free	AF1062-SP

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NBP2-37368

CD7 Antibody (5B10E3) - BSA Free

(1 Review)

0.1 ml

NBP2-37368

Western Blot: CD7 Antibody (5B10E3) [NBP2-37368] - Western blot analysis using CD7 mAb against human CD7 recombinant protein. (Expected MW is 36.6 kDa)

ELISA: CD7 Antibody (5B10E3) [NBP2-37368] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Imaging Mass Cytometry: CD7 Antibody (5B10E3) [NBP2-37368] - FFPE human tonsil tissue, CD7 conjugated to 173Yb. IMC image submitted by a verified customer review.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-37368-0.025ml

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AF7579

CD7 Antibody

(2 Publications)

100 μg

AF7579

HAF016). A specific band was detected for CD7 at approximately 35-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and human peripheral blood lymphocytes (PBL). PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human CD7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7579) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for CD7 at approximately 35-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and human peripheral blood lymphocytes (PBL). PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human CD7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7579) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD7 at approximately 35-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

FAB100A) and either (A) Sheep Anti-Human CD7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7579) or (B) Sheep IgG Control (Catalog # 5-001-A) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126)." class="big_lightbox">

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # <a class=

FAB100A) and either (A) Sheep Anti-Human CD7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7579) or (B) Sheep IgG Control (Catalog # 5-001-A) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126)." class="big_thumb" />

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

CD7 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Sheep Anti-Human CD7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7579) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # <a class=

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CD7 was detected in immersion fixed paraffin-embedded sections of human thymus using Sheep Anti-Human CD7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7579) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF7579U-100UG
Alexa Fluor 405	AF7579V-100UG
Alexa Fluor 488	AF7579G-100UG
Alexa Fluor 532	AF7579X-100UG
Alexa Fluor 594	AF7579T-100UG
Alexa Fluor 647	AF7579R-100UG
Alexa Fluor 700	AF7579N-100UG
Alexa Fluor 750	AF7579S-100UG
Alexa Fluor Plus 405	AF7579AFP405-100UG
Alexa Fluor Plus 488	AF7579AFP488-100UG
Alexa Fluor Plus 555	AF7579AFP555-100UG
Alexa Fluor Plus 594	AF7579AFP594-100UG
Alexa Fluor Plus 647	AF7579AFP647-100UG
Alexa Fluor Plus 680	AF7579AFP680-100UG
Allophycocyanin	AF7579A
Biotin	AF7579B
CoraFluor 1	AF7579CL1
DyLight 350	AF7579D
DyLight 405	AF7579E
DyLight 488	AF7579K
DyLight 550	AF7579L
DyLight 594	AF7579M
DyLight 650	AF7579W
DyLight 680	AF7579Y
DyLight 755	AF7579Z
FITC	AF7579F
HRP	AF7579H
Janelia Fluor 525	AF7579JF525
Janelia Fluor 549	AF7579I
Janelia Fluor 646	AF7579J
Janelia Fluor 669	AF7579JF669
PE	AF7579P
PerCP	AF7579C
mFluor Violet 450 SE	AF7579MFV450
mFluor Violet 500 SE	AF7579MFV500
mFluor Violet 610 SE	AF7579MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF7579-SP

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NBP2-37367

CD7 Antibody (4D4F8) - BSA Free

0.1 ml

NBP2-37367

Flow Cytometry: CD7 Antibody (4D4F8) [NBP2-37367] - Analysis of Jurkat cells using CD7 mouse mAb (green) and negative control (red).

Western Blot: CD7 Antibody (4D4F8) [NBP2-37367] - Analysis using CD7 mAb against human CD7 recombinant protein. (Expected MW is 36.6 kDa).

ELISA: CD7 Antibody (4D4F8) [NBP2-37367] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-37367-0.025ml

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NB500-326

CD7 Antibody (MEM-186) - BSA Free

0.1 mg

NB500-326

Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - [NB500-326-Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) FITC.

Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) PE-CyTM7.

Separation of human CD7 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in Analysis (surface staining) of human peripheral whole blood stained using anti-human CD7 (MEM-186) purified antibody (concentration in sample 0,33 ug/ml, GAM APC).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB500-326AF350
Alexa Fluor 405	NB500-326AF405
Alexa Fluor 488	NB500-326AF488
Alexa Fluor 532	NB500-326AF532
Alexa Fluor 594	NB500-326AF594
Alexa Fluor 647	NB500-326AF647
Alexa Fluor 700	NB500-326AF700
Alexa Fluor 750	NB500-326AF750
Alexa Fluor Plus 405	NB500-326AFP405
Alexa Fluor Plus 488	NB500-326AFP488
Alexa Fluor Plus 555	NB500-326AFP555
Alexa Fluor Plus 594	NB500-326AFP594
Alexa Fluor Plus 647	NB500-326AFP647
Alexa Fluor Plus 680	NB500-326AFP680
Allophycocyanin	NB500-326APC
Allophycocyanin/Cy7	NB500-326APCCY7
Biotin	NB500-326B
CoraFluor 1	NB500-326CL1
DyLight 350	NB500-326UV
DyLight 405	NB500-326V
DyLight 488	NB500-326G
DyLight 550	NB500-326R
DyLight 594	NB500-326DL594
DyLight 650	NB500-326C
DyLight 680	NB500-326FR
DyLight 755	NB500-326IR
FITC	NB500-326F
HRP	NB500-326H
Janelia Fluor 525	NB500-326JF525
Janelia Fluor 549	NB500-326JF549
Janelia Fluor 646	NB500-326JF646
Janelia Fluor 669	NB500-326JF669
PE	NB500-326PE
PE/Atto594	NB500-326PEATT594
PE/Cy5.5	NB500-326PECY55
PE/Cy7	NB500-326PECY7
PerCP	NB500-326PCP
mFluor Violet 450 SE	NB500-326MFV450
mFluor Violet 500 SE	NB500-326MFV500
mFluor Violet 610 SE	NB500-326MFV610

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NBP3-20452

CD7 Antibody (rCD7/6972)

100 μg

NBP3-20452

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox">

CD7 was detected in immersion fixed paraffin-embedded sections of human T-cell Acute Lymphoblastic Lymphoma using Mouse Anti-Human CD7, Monoclonal Antibody (Catalog # NBP3-20452) at 0.2ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="CD7 was detected in immersion fixed paraffin-embedded sections of human T-cell Acute Lymphoblastic Lymphoma using Mouse Anti-Human CD7, Monoclonal Antibody (Catalog # NBP3-20452) at 0.2ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox">

CD7 was detected in immersion fixed paraffin-embedded sections of human appendix using Mouse Anti-Human CD7, Monoclonal Antibody (Catalog # NBP3-20452) at 0.2ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox">

CD7 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CD7, Monoclonal Antibody (Catalog #NBP3-20452) at 0.2ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox">

CD7 was detected in immersion fixed paraffin-embedded sections of human thymus using Mouse Anti-Human CD7, Monoclonal Antibody (Catalog #NBP3-20452) at 0.2ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox">

CD7 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD7, Monoclonal Antibody (Catalog #NBP3-20452) at 0.2ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

Formalin-fixed, paraffin-embedded human tonsil stained with CD7 antibody (rCD7/6972). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human lymph node stained with CD7 antibody (rCD7/6972). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-20944AF350
Alexa Fluor 405	NBP3-20944AF405
Alexa Fluor 488	NBP3-20944AF488
Alexa Fluor 532	NBP3-20944AF532
Alexa Fluor 594	NBP3-20944AF594
Alexa Fluor 647	NBP3-20944AF647
Alexa Fluor 700	NBP3-20944AF700
Alexa Fluor 750	NBP3-20944AF750
Alexa Fluor Plus 405	NBP3-20944AFP405
Alexa Fluor Plus 488	NBP3-20944AFP488
Alexa Fluor Plus 555	NBP3-20944AFP555
Alexa Fluor Plus 594	NBP3-20944AFP594
Alexa Fluor Plus 647	NBP3-20944AFP647
Alexa Fluor Plus 680	NBP3-20944AFP680
Allophycocyanin	NBP3-20944APC
Biotin	NBP3-20944B
CoraFluor 1	NBP3-20944CL1
DyLight 350	NBP3-20944UV
DyLight 405	NBP3-20944V
DyLight 488	NBP3-20944G
DyLight 550	NBP3-20944R
DyLight 594	NBP3-20944DL594
DyLight 650	NBP3-20944C
DyLight 680	NBP3-20944FR
DyLight 755	NBP3-20944IR
FITC	NBP3-20944F
HRP	NBP3-20944H
Janelia Fluor 525	NBP3-20944JF525
Janelia Fluor 549	NBP3-20944JF549
Janelia Fluor 646	NBP3-20944JF646
Janelia Fluor 669	NBP3-20944JF669
PE	NBP3-20944PE
PerCP	NBP3-20944PCP
mFluor Violet 450 SE	NBP3-20944MFV450
mFluor Violet 500 SE	NBP3-20944MFV500
mFluor Violet 610 SE	NBP3-20944MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-20944

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MAB7579

CD7 Antibody (848438) [Unconjugated]

100 μg

MAB7579

FAB100A) and either (A) Mouse Anti-Human CD7 Monoclonal Antibody (Catalog # MAB7579) or (B) Mouse IgG_2BFlow Cytometry Isotype Control (MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B)." class="big_lightbox">

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (<a class=

FAB100A) and either (A) Mouse Anti-Human CD7 Monoclonal Antibody (Catalog # MAB7579) or (B) Mouse IgG_2BFlow Cytometry Isotype Control (MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B)." alt="Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A) and either (A) Mouse Anti-Human CD7 Monoclonal Antibody (Catalog # MAB7579) or (B) Mouse IgG_2BFlow Cytometry Isotype Control (MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B)." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB7579U-100UG
Alexa Fluor 405	FAB7579V-100UG
Alexa Fluor 488	FAB7579G-100UG
Alexa Fluor 532	FAB7579X
Alexa Fluor 594	FAB7579T-100UG
Alexa Fluor 647	FAB7579R-100UG
Alexa Fluor 700	FAB7579N-100UG
Alexa Fluor 750	FAB7579S-100UG
Alexa Fluor Plus 405	FAB7579AFP405-100UG
Alexa Fluor Plus 488	FAB7579AFP488-100UG
Alexa Fluor Plus 555	FAB7579AFP555-100UG
Alexa Fluor Plus 594	FAB7579AFP594-100UG
Alexa Fluor Plus 647	FAB7579AFP647-100UG
Alexa Fluor Plus 680	FAB7579AFP680-100UG
Allophycocyanin/Cy7	FAB7579APCCY7
Biotin	FAB7579B
Biotin	FAB7579B-100
CoraFluor 1	FAB7579CL1
DyLight 350	FAB7579D
DyLight 405	FAB7579E
DyLight 488	FAB7579K
DyLight 550	FAB7579L
DyLight 594	FAB7579M
DyLight 650	FAB7579W
DyLight 680	FAB7579Y
DyLight 755	FAB7579Z
FITC	FAB7579F
HRP	FAB7579H
Janelia Fluor 525	FAB7579JF525
Janelia Fluor 549	FAB7579I
Janelia Fluor 646	FAB7579J
Janelia Fluor 669	FAB7579JF669
PE/Atto594	FAB7579PEATT594
PE/Cy5.5	FAB7579PECY55
PE/Cy7	FAB7579PECY7
PerCP	FAB7579C
mFluor Violet 450 SE	FAB7579MFV450
mFluor Violet 500 SE	FAB7579MFV500
mFluor Violet 610 SE	FAB7579MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB7579-SP
Carrier Free	FAB7579G-100UG
Carrier Free	FAB7579V-100UG
Carrier Free	FAB7579U-100UG
Carrier Free	FAB7579T-100UG
Carrier Free	FAB7579S-100UG
Carrier Free	FAB7579R-100UG
Carrier Free	FAB7579N-100UG
Carrier Free	FAB7579B
Carrier Free	FAB7579B-100

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NBP2-32097

CD7 Antibody - BSA Free

100 ul

NBP2-32097

<b>Biological Strategies Validation. </b>Western Blot: CD7 Antibody [NBP2-32097] - Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD7 antibody NBP2-32097 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-32097AF350
Alexa Fluor 405	NBP2-32097AF405
Alexa Fluor 488	NBP2-32097AF488
Alexa Fluor 532	NBP2-32097AF532
Alexa Fluor 594	NBP2-32097AF594
Alexa Fluor 647	NBP2-32097AF647
Alexa Fluor 700	NBP2-32097AF700
Alexa Fluor 750	NBP2-32097AF750
Alexa Fluor Plus 405	NBP2-32097AFP405
Alexa Fluor Plus 488	NBP2-32097AFP488
Alexa Fluor Plus 555	NBP2-32097AFP555
Alexa Fluor Plus 594	NBP2-32097AFP594
Alexa Fluor Plus 647	NBP2-32097AFP647
Alexa Fluor Plus 680	NBP2-32097AFP680
Allophycocyanin	NBP2-32097APC
Biotin	NBP2-32097B
CoraFluor 1	NBP2-32097CL1
DyLight 350	NBP2-32097UV
DyLight 405	NBP2-32097V
DyLight 488	NBP2-32097G
DyLight 550	NBP2-32097R
DyLight 594	NBP2-32097DL594
DyLight 650	NBP2-32097C
DyLight 680	NBP2-32097FR
DyLight 755	NBP2-32097IR
FITC	NBP2-32097F
HRP	NBP2-32097H
Janelia Fluor 525	NBP2-32097JF525
Janelia Fluor 549	NBP2-32097JF549
Janelia Fluor 646	NBP2-32097JF646
Janelia Fluor 669	NBP2-32097JF669
PE	NBP2-32097PE
PerCP	NBP2-32097PCP
mFluor Violet 450 SE	NBP2-32097MFV450
mFluor Violet 500 SE	NBP2-32097MFV500
mFluor Violet 610 SE	NBP2-32097MFV610

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10214-CD

Recombinant Cynomolgus Monkey CD7 His-tag Protein, CF

50 μg

10214-CD

7559-ST) is immobilized at 0.5 µg/mL, Recombinant Cynomolgus Monkey CD7 His-tag (Catalog # 10214-CD) binds with an ED₅₀ of 8-40 ng/mL." class="big_lightbox">

When Recombinant Human SECTM1 Fc Chimera (Catalog # <a class=

7559-ST) is immobilized at 0.5 µg/mL, Recombinant Cynomolgus Monkey CD7 His-tag (Catalog # 10214-CD) binds with an ED₅₀ of 8-40 ng/mL." alt="When Recombinant Human SECTM1 Fc Chimera (Catalog # 7559-ST) is immobilized at 0.5 µg/mL, Recombinant Cynomolgus Monkey CD7 His-tag (Catalog # 10214-CD) binds with an ED₅₀ of 8-40 ng/mL." class="big_thumb" />

2 μg/lane of Recombinant Cynomolgus Monkey CD7 His-tag (Catalog # 10214-CD)was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 37-44 kDa and 30-40 kDa, respectively.

Reactivity:

Pm-Cm

Species Glossary

Reactivity: Pm-Cm

Applications:

Applications:

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NBP2-89748

CD7 Antibody (15) - Azide and BSA Free

100 ul

NBP2-89748

Reactivity:

Hu, Mu(-), Rt(-)

Species Glossary

Reactivity: Hu, Mu(-), Rt(-)

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-89748AF350
Alexa Fluor 405	NBP2-89748AF405
Alexa Fluor 488	NBP2-89748AF488
Alexa Fluor 532	NBP2-89748AF532
Alexa Fluor 594	NBP2-89748AF594
Alexa Fluor 647	NBP2-89748AF647
Alexa Fluor 700	NBP2-89748AF700
Alexa Fluor 750	NBP2-89748AF750
Alexa Fluor Plus 405	NBP2-89748AFP405
Alexa Fluor Plus 488	NBP2-89748AFP488
Alexa Fluor Plus 555	NBP2-89748AFP555
Alexa Fluor Plus 594	NBP2-89748AFP594
Alexa Fluor Plus 647	NBP2-89748AFP647
Alexa Fluor Plus 680	NBP2-89748AFP680
Allophycocyanin	NBP2-89748APC
Biotin	NBP2-89748B
CoraFluor 1	NBP2-89748CL1
DyLight 350	NBP2-89748UV
DyLight 405	NBP2-89748V
DyLight 488	NBP2-89748G
DyLight 550	NBP2-89748R
DyLight 594	NBP2-89748DL594
DyLight 650	NBP2-89748C
DyLight 680	NBP2-89748FR
DyLight 755	NBP2-89748IR
FITC	NBP2-89748F
HRP	NBP2-89748H
Janelia Fluor 525	NBP2-89748JF525
Janelia Fluor 549	NBP2-89748JF549
Janelia Fluor 646	NBP2-89748JF646
Janelia Fluor 669	NBP2-89748JF669
PE	NBP2-89748PE
PerCP	NBP2-89748PCP
mFluor Violet 450 SE	NBP2-89748MFV450
mFluor Violet 500 SE	NBP2-89748MFV500
mFluor Violet 610 SE	NBP2-89748MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-89748-50ul

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NBP2-81050

CD7 Antibody (3A1E) - Chimeric - Azide and BSA Free

0.2 mg

NBP2-81050

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-81050AF350
Alexa Fluor 405	NBP2-81050AF405
Alexa Fluor 488	NBP2-81050AF488
Alexa Fluor 532	NBP2-81050AF532
Alexa Fluor 594	NBP2-81050AF594
Alexa Fluor 647	NBP2-81050AF647
Alexa Fluor 700	NBP2-81050AF700
Alexa Fluor 750	NBP2-81050AF750
Alexa Fluor Plus 405	NBP2-81050AFP405
Alexa Fluor Plus 488	NBP2-81050AFP488
Alexa Fluor Plus 555	NBP2-81050AFP555
Alexa Fluor Plus 594	NBP2-81050AFP594
Alexa Fluor Plus 647	NBP2-81050AFP647
Alexa Fluor Plus 680	NBP2-81050AFP680
Allophycocyanin	NBP2-81050APC
Biotin	NBP2-81050B
CoraFluor 1	NBP2-81050CL1
DyLight 350	NBP2-81050UV
DyLight 405	NBP2-81050V
DyLight 488	NBP2-81050G
DyLight 550	NBP2-81050R
DyLight 594	NBP2-81050DL594
DyLight 650	NBP2-81050C
DyLight 680	NBP2-81050FR
DyLight 755	NBP2-81050IR
FITC	NBP2-81050F
HRP	NBP2-81050H
Janelia Fluor 525	NBP2-81050JF525
Janelia Fluor 549	NBP2-81050JF549
Janelia Fluor 646	NBP2-81050JF646
Janelia Fluor 669	NBP2-81050JF669
PE	NBP2-81050PE
PerCP	NBP2-81050PCP
mFluor Violet 450 SE	NBP2-81050MFV450
mFluor Violet 500 SE	NBP2-81050MFV500
mFluor Violet 610 SE	NBP2-81050MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-81050-0.025mg

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NBP2-44799

CD7 Antibody (B-F12)

0.1 mg

NBP2-44799

Western blot analysis of HepG2 cell lysates using CD7 Antibody (B-F12).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

PDGFR alpha Antibody [Unconjugated]

CD7 Antibody (5B10E3) - BSA Free

CD7 Antibody

CD7 Antibody (4D4F8) - BSA Free

CD7 Antibody (MEM-186) - BSA Free

CD7 Antibody (rCD7/6972)

CD7 Antibody (848438) [Unconjugated]

CD7 Antibody - BSA Free

Recombinant Cynomolgus Monkey CD7 His-tag Protein, CF

CD7 Antibody (15) - Azide and BSA Free

CD7 Antibody (3A1E) - Chimeric - Azide and BSA Free

CD7 Antibody (B-F12)