DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder." class="big_lightbox">
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder." alt="CD7 was detected in immersion fixed paraffin-embedded sections of human T-cell Acute Lymphoblastic Lymphoma using Mouse Anti-Human CD7, Monoclonal Antibody (Catalog # NBP3-20452) at 0.2ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog #
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder." class="big_thumb" />
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder." class="big_lightbox">
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder." class="big_thumb" />
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder.​" class="big_lightbox">
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder.​" class="big_thumb" />
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder.​" class="big_lightbox">
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder.​" class="big_thumb" />
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder.​" class="big_lightbox">
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder.​" class="big_thumb" />
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="CD7 was detected in immersion fixed paraffin-embedded sections of human T-cell Acute Lymphoblastic Lymphoma using Mouse Anti-Human CD7, Monoclonal Antibody (Catalog # NBP3-20452) at 0.2ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />
![Western Blot: CD7 Antibody (5B10E3) [NBP2-37368] - Western blot analysis using CD7 mAb against HEK293 (1) and CD7 (AA: 72-175)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD7-Antibody-5B10E3-Western-Blot-NBP2-37368-img0003.jpg "Western Blot: CD7 Antibody (5B10E3) [NBP2-37368] - Western blot analysis using CD7 mAb against HEK293 (1) and CD7 (AA: 72-175)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: CD7 Antibody (5B10E3) [NBP2-37368] - Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using CD7 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD7-Antibody-5B10E3-Immunohistochemistry-NBP2-37368-img0004.jpg)
![Western Blot: CD7 Antibody (5B10E3) [NBP2-37368] - Western blot analysis using CD7 mAb against human CD7 recombinant protein. (Expected MW is 36.6 kDa)](http://images.novusbio.com/fullsize/CD7-Antibody-5B10E3-Western-Blot-NBP2-37368-img0002.jpg)
![ELISA: CD7 Antibody (5B10E3) [NBP2-37368] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CD7-Antibody-5B10E3-ELISA-NBP2-37368-img0001.jpg)
![Imaging Mass Cytometry: CD7 Antibody (5B10E3) [NBP2-37368] - FFPE human tonsil tissue, CD7 conjugated to 173Yb. IMC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD7-Antibody-5B10E3-Imaging-Mass-Cytometry-NBP2-37368-img0005.jpg)
![Western Blot: CD7 Antibody (4D4F8) [NBP2-37367] - Analysis using CD7 mAb against HEK293 (1) and CD7 (AA: 72-175)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD7-Antibody-4D4F8-Western-Blot-NBP2-37367-img0003.jpg "Western Blot: CD7 Antibody (4D4F8) [NBP2-37367] - Analysis using CD7 mAb against HEK293 (1) and CD7 (AA: 72-175)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: CD7 Antibody (4D4F8) [NBP2-37367] - Analysis of lung cancer tissues using CD7 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD7-Antibody-4D4F8-Immunohistochemistry-NBP2-37367-img0005.jpg)
![Flow Cytometry: CD7 Antibody (4D4F8) [NBP2-37367] - Analysis of Jurkat cells using CD7 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CD7-Antibody-4D4F8-Flow-Cytometry-NBP2-37367-img0004.jpg)
![Western Blot: CD7 Antibody (4D4F8) [NBP2-37367] - Analysis using CD7 mAb against human CD7 recombinant protein. (Expected MW is 36.6 kDa).](http://images.novusbio.com/fullsize/CD7-Antibody-4D4F8-Western-Blot-NBP2-37367-img0002.jpg)
![ELISA: CD7 Antibody (4D4F8) [NBP2-37367] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CD7-Antibody-4D4F8-ELISA-NBP2-37367-img0001.jpg)
![Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/CD7-Antibody-MEM-186-Flow-Cytometry-NB500-326-img0004.jpg "Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) PerCP-CyTM5.5.")
![Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) APC.](http://images.novusbio.com/fullsize/CD7-Antibody-MEM-186-Flow-Cytometry-NB500-326-img0001.jpg)
![Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - [NB500-326-Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) FITC.](http://images.novusbio.com/fullsize/CD7-Antibody-MEM-186-Flow-Cytometry-NB500-326-img0002.jpg)
![Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) PE-CyTM7.](http://images.novusbio.com/fullsize/CD7-Antibody-MEM-186-Flow-Cytometry-NB500-326-img0003.jpg)
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # 
![Western Blot: CD7 Antibody [NBP2-32097] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD7 antibody NBP2-32097 used for IP at 6 ug per reaction. For blotting immunoprecipitated CD7, NBP2-32097 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CD7-Antibody-Western-Blot-NBP2-32097-img0002.jpg "Western Blot: CD7 Antibody [NBP2-32097] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD7 antibody NBP2-32097 used for IP at 6 ug per reaction. For blotting immunoprecipitated CD7, NBP2-32097 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry: CD7 Antibody [NBP2-32097] - Sample: FFPE section of human stomach cancer infiltrating lymphocytes. Antibody: Affinity purified rabbit anti- CD7 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/CD7-Antibody-Immunohistochemistry-NBP2-32097-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CD7 Antibody [NBP2-32097] - Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD7 antibody NBP2-32097 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CD7-Antibody-Western-Blot-NBP2-32097-img0001.jpg)
![Immunohistochemistry-Paraffin: CD7 Antibody (015) [NBP2-89748] - Staining of human CD7 in human tonsil with rabbit monoclonal antibody (1:200). Positive staining was localized to membrane of T cells.](http://images.novusbio.com/fullsize/CD7-Antibody-015-Immunohistochemistry-Paraffin-NBP2-89748-img0001.jpg "Immunohistochemistry-Paraffin: CD7 Antibody (015) [NBP2-89748] - Staining of human CD7 in human tonsil with rabbit monoclonal antibody (1:200). Positive staining was localized to membrane of T cells.")
![Immunohistochemistry-Paraffin: CD7 Antibody (015) [NBP2-89748] - Staining of human CD7 in human lymphoid node with NBP2-89748 (1:200). Positive staining was localized to membrane of T cells.](http://images.novusbio.com/fullsize/nbp2-89748_rabbit-monoclonal-cd7-antibody-015-23520251125445.png)
![Western Blot: CD7 Antibody (3A1E) [NBP2-81050] - Western Blot using anti-CD7 antibody (3A1E) [NBP2-81050]. Jurkat cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 3A1E [NBP2-81050] at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified CD7 is 25.4kDa, but this protein is glycosylated at several residues. NBP2-81050 successfully detected CD7 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/CD7-Antibody-3A1E-Western-Blot-NBP2-81050-img0002.jpg "Western Blot: CD7 Antibody (3A1E) [NBP2-81050] - Western Blot using anti-CD7 antibody (3A1E) [NBP2-81050]. Jurkat cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 3A1E [NBP2-81050] at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified CD7 is 25.4kDa, but this protein is glycosylated at several residues. NBP2-81050 successfully detected CD7 in Jurkat cell lysate.")
![Immunocytochemistry/Immunofluorescence: CD7 Antibody (3A1E) [NBP2-81050] - Immunofluorescence staining of fixed Jurkat cells with anti-CD7 antibody (3A1E) [NBP2-81050]. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 3A1E [NBP2-81050] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81050, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/CD7-Antibody-3A1E-Immunocytochemistry-Immunofluorescence-NBP2-81050-img0001.jpg)
