Product Search for Beta Tubulin: Novus Biologicals

MAB1195

beta-III Tubulin Antibody (TuJ-1) [Unconjugated] - Neuron-Specific

(8 Reviews)

100 μg

MAB1195

MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules." class="big_lightbox">

MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules." alt="A172 cells were stained with Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules." class="big_thumb" />

HAF018). A specific band was detected for beta -III Tubulin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human brain (cerebellum) tissue, human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and mouse brain (stem) tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Neuron-specific beta -III Tubulin Monoclonal Antibody (Catalog # MAB1195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

HAF018). A specific band was detected for beta -III Tubulin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

AF2594). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta-III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and for GFAP using the NorthernLights 493-conjugated Anti-Sheep IgG Secondary Antibody (green; NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

beta -III Tubulin was detected in rat cortical neurons using 5 µg/mL neuron-specific Mouse Anti-Neuron-specific beta -III Tubulin Monoclonal Antibody (Catalog # MAB1195). GFAP was detected in rat astrocytes using 10 µg/mL Human GFAP Antigen Affinity-purified Polyclonal Antibody (<a class=

AF2594). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta-III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and for GFAP using the NorthernLights 493-conjugated Anti-Sheep IgG Secondary Antibody (green; NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

beta ‑III Tubulin was detected in immersion fixed differentiated human neural progenitor cells using Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (clone TuJ-1) (Catalog # MAB1195) for 3 hours at room temperature. Cells were stained (green) and counterstained (red). View our protocol for <a class=

Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NSC001) using 5 µg/mL neuron-specific Mouse Anti-Neuron-specific beta -III Tubulin Monoclonal Antibody (Catalog # MAB1195) and 10 µg/mL Rat Nestin Antigen Affinity-purified Polyclonal Antibody (AF2736). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta-III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and for Nestin using the NorthernLights 493-conjugated Anti-Goat IgG Secondary Antibody (green; NL003). Tissue was counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

beta -III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # <a class=

NSC001) using 5 µg/mL neuron-specific Mouse Anti-Neuron-specific beta -III Tubulin Monoclonal Antibody (Catalog # MAB1195) and 10 µg/mL Rat Nestin Antigen Affinity-purified Polyclonal Antibody (AF2736). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta-III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and for Nestin using the NorthernLights 493-conjugated Anti-Goat IgG Secondary Antibody (green; NL003). Tissue was counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

beta -III Tubulin was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Neuron-specific beta -III Tubulin Monoclonal Antibody (Catalog # MAB1195) at 8 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Simple Western lane view shows lysates of rat cortical neurons, loaded at 0.2 mg/mL. A specific band was detected for beta ‑III Tubulin at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

beta ‑III Tubulin was detected in immersion fixed SH‑SY5Y human neuroblastoma cells (Positive) & absent in HEL 92.1.7 human erythroleukemic cells (Negative) using Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195) at 0.5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

Representative immunocytochemistry demonstrating the expression of neuronal, synaptic, glutamatergic, GABAergic, and glial markers in cultures 21 days after plating. Neuronal cultures were immunostained against beta -III Tubulin (A–F); SNAP-25 (A); SV2 isoform A–C (A,B); polysialo gangliosides GD1a and GT1b/2b (C) Syn1 (D,E); glutamatergic neurons (VGLUT2, D); GABAergic neurons (GAD1, E) and glial cells (GFAP, F). Shown also are DAPI nuclear staining and the merged images. Scale bar is 10 μm (A–E), respectively, 25 μm for the bottom panel (F). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28280466), licensed under a CC-BY license. Not internally tested by R&D Systems.

Neuronal markers expressed by iPSC-NSCs after differentiation and maturation on PEG hydrogels.(a) beta III-tubulin (red), GAD 65/67 (green), and DAPI (nuclei, blue). Single channel grayscale images from (a) are shown for (b) beta III-tubulin and (c) GAD 65/67. (d) VGLUT2 (red), MAP2 (green), and DAPI (nuclei, blue). Single channel grayscale images from (d) are shown for (e) VGLUT2 and (f) MAP2. (g) beta III-tubulin (red), GABA (green), and DAPI (nuclei, blue). Single channel grayscale images from (g) are shown for (h) beta III-tubulin and (i) GABA. (j) MNX1/HB9 (green) and DAPI (nuclei, blue). Single channel grayscale images from (j) are shown for (k) MNX1/HB9 and (l) DAPI. (m) beta III-tubulin (red), Synapsophysin (green), and DAPI (nuclei, blue). Single channel grayscale images from (m) are shown for (n) beta III-tubulin and (o) Synapsophysin. (p) Synapsin-1 (green) and DAPI (nuclei, blue). Single channel grayscale images from (p) are shown for (q) Synapsin-1 and (r) DAPI. Scale Bars: 200 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26411797), licensed under a CC-BY license. Not internally tested by R&D Systems.

I alpha I maintains pluripotency and differentiation capacity in long-term culture.Immunofluorescence staining of H207 hES cell line for (a) stem cell markers Oct4 (green), Sox2 (green), Nanog (red) and SSEA-4 (green) after 16 passages in E8:I alpha I, and for the three germ layers after 4 weeks of differentiation through embryoid body (EB) formation: mesoderm with alpha smooth muscle actin (SMA, green), ectoderm with Nestin (red) and beta -III-tubulin (green) and endoderm with alpha-fetoprotein (AFP, green) and PDGF-receptor (red), and (b) after endoderm directed differentiation for endoderm markers Sox7 (red), Sox17 (green) and HNF3 beta (green) and stem cell markers Oct4 (green) and Nanog (red), as well as negative control with secondary antibodies. All samples were co-stained with DAPI (Blue) for nuclei detection. Scale bars show 100 μm, white boxes show close-up images. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms12170), licensed under a CC-BY license. Not internally tested by R&D Systems.

Characterization of NSCs and cortical neurons derived from FKRP‐ and CRISPR/Cas9 corrected‐iPSCsA, BRepresentative images of NSCs derived from FKRP‐ and corrected‐iPSC lines expressing SOX1, SOX2, and nestin.C, DQuantification of percentage of SOX1+ (C) and SOX2+ (D) cells in culture. The efficiency of neural induction is more than 99% in FKRP‐ and corrected‐iPSC (5D17, 5D23, and 3B17) lines. Data are mean ± s.d. n = 4 technical replicates.E, FFKRP‐ and corrected‐NSC lines can be further differentiated to cortical neural progenitor cells, expressing PAX6, OTX2, and vimentin.G–IQuantification of percentage of PAX6+ (G) and OTX2+ (H) cells in culture. About 91‐98% of cells derived from FKRP, 5D17, 5D23, and 3B17 NSC lines express PAX6 (G). About 93‐96% of cells derived from FKRP, 5D17, 5D23, and 3B17 NSC lines express OTX2 (H). Of the OTX2+ population, about 60‐67% cells are also Ki67+ cycling progenitors (I). Data are mean ± s.d. n = 4 technical replicates.J, KGlutamatergic projection neurons derived from FKRP and corrected (5D17, 5D23, and 3B17) progenitor cells. The vast majority of neurons contain vGlut1+ punctae in their neurites (labeled by Tuj1). Right panels are enlarged images from the insets of left panels.Data information: Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31566294), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of NSUN2 in the Human Developing Brain and NES Cells(A) DAPI-stained human embryo (6 weeks of gestation) marked for prosencephalon, mesencephalon, and rhombencephalon. Region in square is magnified in (B). Scale bar, 1 mm.(B) Prosencephalon labeled for NSUN2 and SOX1. Region in squares are magnified in (b′) and (b″). Arrows indicate NSUN2-positive cells. Scale bar, 100 μm.(C–F) Bright-field image (C) and immunofluorescence (D–F) of AF22 (upper panels) and Sai1 (lower panels) cells labeled for Nestin (D), SOX2 (E), and beta III-tubulin (F). Scale bar, 50 μm.(G and H) NES cells co-labeled for NSUN2 and Nestin (NES) (G) or SOX1 (H).(I) Differentiation protocol.(J–L) Differentiated AF22 and Sai1 cells (day 15) labeled for Nestin (NES; J), SOX2 (K), and beta III-tubulin (L). Scale bars: 50 μm.(M) Western blot for NSUN2, beta III-tubulin (TUBB3), GFAP, SOX2, and Nestin during differentiation (days). alpha -Tubulin served as loading control.Nuclei are counterstained with DAPI (A, B, D–F, J–L). Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213671116302764), licensed under a CC-BY license. Not internally tested by R&D Systems.

Functional glycosylation of alpha ‐dystroglycan and characterization of dystroglycanopathy patient‐specific iPSCsCurrent model of the core M3 functional glycan structure on alpha ‐dystroglycan and enzymes involved in its synthesis. ECM ligands, such as laminins, bind to the Xyl‐GlucA disaccharide repeats (IIH6 epitope). Man, mannose; GlcNAc, N‐acetylglucosamine; GalNAc, N‐acetylgalactosamine; Rbo5P, ribitol‐5‐phosphate; Xyl, xylose; GlcA, glucuronic acid.Representative images of immunostaining demonstrate that FKRP‐iPSCs express specific pluripotency‐associated markers, including NANOG, OCT4, Tra‐1‐60, and SSEA4.FKRP‐iPSCs have a normal karyotype.In vitro differentiation of FKRP‐iPSCs to cells representing ectoderm ( beta ‐III Tubulin, Tuj1), mesoderm (SMA, smooth muscle actin), and endoderm (AFP, alpha ‐fetoprotein).Data information: Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31566294), licensed under a CC-BY license. Not internally tested by R&D Systems.

Differentiation of murine skin stem cell precursors (SKP).The differentiation of SKPs was induced through the dissociation of cell aggregates into single cells followed by serum exposure and, as may be seen in the micrographs after 7 days (A), 14 days (B) and 21 days (C), these cells presented several elongated extensions. The mRNA levels of astrocyte markers (D) GFAP; oligodendrocytes (E) CNPase; and neurons (F) beta III tubulin were measured by qRT-PCR and validated at the protein level (G) by immunofluorescence microscopy, Bar = 50μm. The results are presented as the mean ± SD of values obtained in three independent experiments performed in triplicates. Statistical analyzes were performed using ANOVA analysis of variance followed by Tukey test to post-Kramer. All groups were measured versus the undifferentiated control in the shortest time of differentiation, ns = not significant; * p ≤ 0.05; ** p≤0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0140143), licensed under a CC-BY license. Not internally tested by R&D Systems.

CTCL is associated with nerve innervations in mouse lymphoma.(A) Double immunostaining for CGRP and beta –tubulin III in the back skin with lymphoma from a CTCL mouse at day 20. Dotted lines show the epidermis. Scale bar: 25 μm. (B) Immunostaining for NF200 in the tumor from a CTCL mouse at day 20. Dotted lines show the epidermis. Scale bar: 25 μm. (C) 3D reconstruction of innervated nerves in a beta –tubulin III–labeled tumor from a CTCL mouse at day 20. Scale bar: 300 μm. (D) High-magnification image of the boxed area in C. Scale bar: 150 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36520531), licensed under a CC-BY license. Not internally tested by R&D Systems.

Stabilized gamma -catenin suppresses neuronal differentiation in embryoid body assays and reduces overall tri-lineage differentiation efficiency.(A and B) Embryoid bodies were generated using control, beta -catS33A, and gamma -catS28A mESCs, and assayed for neuronal differentiation after 10 days. (A) Only WT EBs displayed immunofluorescent staining for the neuronal marker, beta -III-tubulin. (B) The transcript levels of the neuronal markers beta -III-tubulin, Nestin, Map2, Tyrosine hydroxylase, the endoderm marker alpha -Fetoprotein, and the mesoderm marker Cardiac Troponin, were reduced in EBs derived from mESCs overexpressing beta -catS33A or gamma -catS28A, as assessed by qRT-PCR analyses. Bars represent means and error bars indicate s.e.m. (n = 2). Size bar = 200 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23724138), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

All-Multi

Species Glossary

Reactivity: All-Multi

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC1195U-100UG
Alexa Fluor 405	IC1195V-100UG
Alexa Fluor 488	IC1195G-100
Alexa Fluor 532	FAB1195X-100UG
Alexa Fluor 594	IC1195T-100UG
Alexa Fluor 647	IC1195R-100UG
Alexa Fluor 700	IC1195N-100UG
Alexa Fluor 750	IC1195S-100UG
Allophycocyanin	IC1195A
Biotin	BAM1195
CoraFluor 1	FAB1195CL1
DyLight 350	FAB1195D
DyLight 405	FAB1195E
DyLight 488	FAB1195K
DyLight 550	FAB1195L
DyLight 594	FAB1195M
DyLight 650	FAB1195W
DyLight 680	FAB1195Y
DyLight 755	FAB1195Z
FITC	FAB1195F
HRP	FAB1195H
Janelia Fluor 525	FAB1195JF525
Janelia Fluor 549	FAB1195I
Janelia Fluor 646	FAB1195J
Janelia Fluor 669	FAB1195JF669
NL493	NL1195G
NL557	NL1195R
NL637	NL1195V
PE	FAB1195P
PerCP (Peridinin-chlorophyll Protein Complex)	IC1195C
mFluor Violet 450 SE	FAB1195MFV450
mFluor Violet 500 SE	FAB1195MFV500
mFluor Violet 610 SE	FAB1195MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC1195V-100UG
Carrier Free	IC1195S-100UG
Carrier Free	IC1195T-100UG
Carrier Free	IC1195U-100UG
Carrier Free	IC1195G-100
Carrier Free	IC1195R-100UG
Carrier Free	IC1195N-100UG
Carrier Free	IC1195C
Carrier Free	IC1195A
Carrier Free	NL1195R
Carrier Free	NL1195G
Carrier Free	NL1195V
Carrier Free	MAB1195-SP

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NB600-936

beta Tubulin Antibody - BSA Free

(7 Reviews)

(97 Publications)

0.1 ml

NB600-936

Western Blot: beta Tubulin Antibody [NB600-936] - Analysis of extracts from HeLa, A549 and Jurkat cells using NB600-936 Beta-tubulin antibody at 1:1000

<b>Biological Strategies Validation. </b>beta-Tubulin-Antibody-Western-Blot-NB600-936-img0020.jpg

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-beta Tubulin Antibody (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human skin using 1:2000 dilution of beta Tubulin antibody. Intense cytoplasmic staining of beta Tubulin (TUBB) protein was observed in various cells of the epidermal as well as the dermal cells [10X Magnification].

Western Blot: beta Tubulin Antibody [NB600-936] - Analysis in HeLa whole cell lysate at a 1:1,000 dilution.

beta-Tubulin-Antibody-Western-Blot-NB600-936-img0024.jpg

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - Confocal immunofluorescent analysis of C2C12 cells using beta Tubulin antibody (NB600-936, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - Analysis of beta Tubulin in mouse hippocampal primary culture. Image courtesy of product review by Lin Yi-Wen.

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-beta Tubulin Antibody (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. Strong cytoplasmic immuno-positivity of beta Tubulin (TUBB) was observed in SCC cells as well as the associated tumor stromal cells [10X Magnification].

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human brain using 1:2000 dilution of beta Tubulin antibody. The various brain cells depicted strong cytoplasmic immunoreactivity of beta Tubulin (TUBB) protein [10X Magnification].

Beta Tubulin was detected in immersion fixed A431 human skin carcinoma cell line using Rabbit anti-beta Tubulin Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NB600-936AF647) (light blue) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Beta Tubulin was detected in immersion fixed U-2 OS human osteosarcoma cell line using Rabbit anti-beta Tubulin Affinity Purified Polyclonal Antibody conjugated to DyLight 488 (Catalog # NB600-936G) (green) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Dual pathway inhibition regulates TK1 protein levels & results in greater p27 protein levels than single agents alone.Single agent PLX4032 resulted in activation of p-AKT Ser473 following 24 hours of exposure at two concentrations (100 nM, 1 µM). The addition of the dual PI3K/mTOR inhibitor BEZ235 blocks p-AKT Ser473 activation & resulted in a greater increase in p27 protein levels & diminished TK1 protein levels. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Expression of pluripotency related markers & a sphere culture of BEAS-2B under normoxia.(a) HIF-2 alpha expression was detected in the nucleus of BEAS-2B under normoxia by western blotting. Beta tubulin & TATA binding protein (TBP) were used as protein markers for the cytosol & nucleus fraction, respectively. (b) Western blotting for Oct-4. (c) For knockdown of HIF-2 alpha , BEAS-2B cells were transfected with shRNA targeting HIF-2 alpha & sh-Luc was used as control. (d) Western blotting of Nanog & a culture of BEAS-2B containing floating spheres. Representative result from two or three experiments was presented. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27373565), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Expression of AAV transgene before & after experimental autoimmune uveoretinitis (EAU) induction. (A) B10.RIII mice were evaluated by funduscopy one month after an intravitreal injection of AAV delivering sGFP-TatM013v5 or secreted GFP (sGFP). As a control we evaluated mice that received an intravitreal injection of saline. Diffuse fluorescence indicates the secretion of sGFP & sGFP-TatM013v5 in the retina. (B) Western blot from retinas harvested 14 days after IRBP immunization. Membrane was probed with anti-GFP & anti-Tubulin antibodies. Image shows expression of sGFP on both sGFP & sGFP-TatM013v5 retina lysates. (n = 2–3 retina samples from different mice per group). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31795515), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Combined V600EBRAF & mTOR inhibition results in transcriptional control of TK1 protein levels in COLO 205 cells.(A) Western blot of COLO 205 cells treated with PP242 (250 nM) & increasing PLX4032. Similar to single agent PLX4032, p-MEK, but not p-ERK, was inhibited in a PLX4032-dependent manner. Consistent with mTORC1/mTORC2 inhibition, p-AKT Ser473, but not p-AKT Thr308, was inhibited. Unlike single agent PLX4032, which resulted in concentration-dependent activation of p-rpS6, combined treatment maintained p-rpS6 levels at essentially baseline levels except at the highest PLX4032 concentration. Similarly, DUSP6 levels were inversely related to p-ERK protein levels. With combined mTOR & V600EBRAF blockade, p27 & TK1 protein levels were inversely correlated & dramatically affected by PLX4032 exposure. (B) Similarly, TK1 mRNA was significantly reduced at PLX4032 concentrations as low as 10 nM. (C) Despite elevated p27 protein levels, p27 mRNA was unaffected by combined mTOR-V600EBRAF inhibition. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - [18F]-FLT PET reflects BEZ235-dependent inhibition of PI3K/mTOR activity in PLX4720 treated COLO 205 xenografts. Xenograft-bearing mice were imaged with [18F]-FLT PET on treatment day 4. (A) [18F]-FLT uptake was diminished in the combination treatment cohort relative to vehicle (p = 0.0087), but not single agent PLX4720- or BEZ235-treated cohorts. (B) Western blot of xenograft tissue harvested immediately following imaging illustrated elevated p-ERK & p-rpS6 levels in PLX4720-treated mice. Combining PLX4032 with BEZ235 resulted in reduced p-ERK & p-rpS6 protein levels. (C) TK1 levels, as measured by IHC, were reduced only in the combination treatment group in agreement with [18F]-FLT PET. (D) Consistent with in vitro studies, diminished TK1 levels, & consequently [18F]-FLT PET, correlated with elevated p27 that was elevated only in the combination treated group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - PLX4720 exposure does not affect [18F]-FLT PET in COLO 205 xenografts, despite evidence of target inhibition & diminished [18F]-FDG uptake.(A) Representative transverse [18F]-FLT & [18F]-FDG PET images acquired after three daily treatments with vehicle or 60 mg/kg PLX4720 (tumor indicated by arrowhead). (B) Quantification of PET data illustrated similar [18F]-FLT uptake in vehicle-treated & PLX4720-treated tumors. Unlike [18F]-FLT PET, PLX4720 exposure elicited a significant reduction in [18F]-FDG uptake (p = 0.0006). (C) Western blot analysis of vehicle- & PLX4720-treated tumor tissue confirmed that PLX4720 had no effect on TK1 protein levels in agreement with [18F]-FLT PET. Target inhibition as measured by p-MEK levels was observed. However, similar to in vitro studies, PLX4720-treated COLO 205 xenografts exhibited elevated p-ERK & p-rpS6 protein levels relative to vehicle controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Deletion of Phb1 affects lipid metabolism.Western blot (a) & quantification (b) of Acetyl-CoA carboxylase (ACC) & phosphorylated ACC (p-ACC) expression at P20. N = 6–7 animals per genotype. Unpaired two-tailed t-test [p-ACC (t = 0.4627, df = 11, p = 0.021), ACC (t = 1.355, df = 11, p = 0.26)]. Western blot (c) & quantification (d) of ACC & p-ACC expression at P40. N = 6–8 animals per genotype [p-ACC (t = 0.5447, df = 12, p = 0.42), ACC (t = 1.153, df = 12, p = 0.17)]. Unpaired two-tailed t-test. By RT-qPCR, we identified a significant downregulation of many enzymes involved with lipid biosynthesis at both P20 (e) & P40 (f): sterol regulatory element-binding protein 1 (Srebp1), 3-hydroxy-3-methylglutaryl-CoA reductase (Hmgcr), ATP citrate lyase (Acly), fatty acid synthase (FASN), acetyl-CoA carboxylase 2 (ACC2), N = 5 animals per genotype. Unpaired two-tailed t-test P20 [Srebp1 (t = 3.26, df = 8, p = 0.012), Hmgcr (t = 7.63, df = 8, p = 0.000061), Acly (t = 4.418, df = 8, 0.0022), FASN (t = 4.109, df = 8, p = 0.0034), ACC2 (t = 3.408, df = 8, p = 0.0092)]; P40 [Srebp1 (t = 7.551, df = 8, p = 0.000066), Hmgcr (t = 5.091, df = 8, p = 0.00094), Acly (t = 4.934, df = 8, p = 0.0011), FASN (t = 7.186, df = 8, p = 0.000094), ACC2 (t = 2.697, df = 8, p = 0.027)]. Data are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. n.s. non-significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34078899), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - TK1 protein levels do not reflect p-ERK attenuation following inhibition of V600EBRAF inhibition in COLO 205 cells.COLO 205 cells were collected 48 hours of PLX 4032 exposure at 10 nM, 100 nM, 500 nM, 1 µM, or 5 µM. (A) Western blot analysis demonstrated target inhibition of p-MEK despite increased p-ERK levels. PI3K-mTOR signaling was elevated in a PLX 4032-dependent manner as exhibited by a steady rise in p-rpS6 levels. The ERK-phosphatase DUSP6 decreased in conjunction with mTOR signaling & was inversely proportional to p-ERK levels. A slight increase in p27 levels were observed concomitantly with only modest changes in TK1 levels, except at the highest dose of PLX4032. (B) Decreased TK1 mRNA levels were observed at all drug concentrations above 10 nM (p<0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Beta Tubulin was detected in immersion fixed NIH-3T3 Mouse fibroblast cell line using Rabbit anti-beta Tubulin Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NB600-936AF647) (light blue) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ce, Ch, ChHa, I, In, Pm, Xp, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ce, Ch, ChHa, I, In, Pm, Xp, Ze

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-936AF350
Alexa Fluor 405	NB600-936AF405
Alexa Fluor 488	NB600-936AF488
Alexa Fluor 532	NB600-936AF532
Alexa Fluor 594	NB600-936AF594
Alexa Fluor 647	NB600-936AF647
Alexa Fluor 700	NB600-936AF700
Alexa Fluor 750	NB600-936AF750
Alexa Fluor Plus 405	NB600-936AFP405
Alexa Fluor Plus 488	NB600-936AFP488
Alexa Fluor Plus 555	NB600-936AFP555
Alexa Fluor Plus 594	NB600-936AFP594
Alexa Fluor Plus 647	NB600-936AFP647
Alexa Fluor Plus 680	NB600-936AFP680
Allophycocyanin	NB600-936APC
Biotin	NB600-936B
CoraFluor 1	NB600-936CL1
DyLight 350	NB600-936UV
DyLight 405	NB600-936V
DyLight 488	NB600-936G
DyLight 550	NB600-936R
DyLight 594	NB600-936DL594
DyLight 650	NB600-936C
DyLight 680	NB600-936FR
DyLight 755	NB600-936IR
FITC	NB600-936F
HRP	NB600-936H
Janelia Fluor 525	NB600-936JF525
Janelia Fluor 549	NB600-936JF549
Janelia Fluor 646	NB600-936JF646
Janelia Fluor 669	NB600-936JF669
PE	NB600-936PE
PerCP	NB600-936PCP
mFluor Violet 450 SE	NB600-936MFV450
mFluor Violet 500 SE	NB600-936MFV500
mFluor Violet 610 SE	NB600-936MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-936SS

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NB100-56459

beta Tubulin Antibody - BSA Free

(11 Publications)

0.1 mg

NB100-56459

Simple Western: beta Tubulin Antibody [NB100-56459] - Image shows a specific band for beta Tubulin in 0.5 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Reactivity:

Hu, Mu, Rt, Bv, Ca, Ch, Fe, Pm, Pm, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Fe, Pm, Pm, Xp

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5810A

Alt Catalog# IMG-5810A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56459SS

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NBP2-37830

beta Tubulin Antibody (BT7R) - Azide and BSA Free

(1 Publication)

100 μg

NBP2-37830

Immunohistochemistry-Paraffin: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis showing staining in the cytoskeleton of human colon carcinoma (right) compared with a negative control without primary antibody (left).

Flow (Intracellular): beta Tubulin Antibody (BT7R) [NBP2-37830] - An intracellular stain was performed on HeLa cells with beta Tubulin Antibody (BT7R) NBP2-37830PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.

Western Blot: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis of mouse brain tissue lysate loaded at 10 ug/lane and detected with 0.25 ug/ml of NBP2-37830.

Western Blot: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis of 8ug of mouse embryonic stem cell lysate onto an SDS-PAGE gel.

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis of Beta-Tubulin (red) in HEK293T cells. Cells fixed in 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a Beta-Tubulin monoclonal antibody at a dilution of 1:100 overnight at 4C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 microscope at 40X magnification. Data courtesy of the Innovators Program.

Immunohistochemistry-Paraffin: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis of Beta-Tubulin showing staining in the cytoskeleton of paraffin-embedded mouse colon tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Beta-Tubulin loading control antibody diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Immunohistochemistry-Paraffin: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis showing staining in the cytoskeleton of paraffin-embedded human lung adenocarcinoma (right) compared with a negative control without primary antibody (left).

Flow Cytometry: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis of Beta Tubulin in NIH-3T3 cells (green) compared to an isotype control (blue).

Flow Cytometry: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis of Beta Tubulin in Hela cells (green) compared to an isotype control (blue).

Flow Cytometry: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis of Beta Tubulin in CEM cells (green) compared to an isotype control (blue).

Reactivity:

Hu, Mu, Rt, Av, Ch, Pm, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Av, Ch, Pm, Rb

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-37830AF350
Alexa Fluor 405	NBP2-37830AF405
Alexa Fluor 488	NBP2-37830AF488
Alexa Fluor 532	NBP2-37830AF532
Alexa Fluor 594	NBP2-37830AF594
Alexa Fluor 647	NBP2-37830AF647
Alexa Fluor 700	NBP2-37830AF700
Alexa Fluor 750	NBP2-37830AF750
Alexa Fluor Plus 405	NBP2-37830AFP405
Alexa Fluor Plus 488	NBP2-37830AFP488
Alexa Fluor Plus 555	NBP2-37830AFP555
Alexa Fluor Plus 594	NBP2-37830AFP594
Alexa Fluor Plus 647	NBP2-37830AFP647
Alexa Fluor Plus 680	NBP2-37830AFP680
Allophycocyanin	NBP2-37830APC
Biotin	NBP2-37830B
CoraFluor 1	NBP2-37830CL1
DyLight 350	NBP2-37830UV
DyLight 405	NBP2-37830V
DyLight 488	NBP2-37830G
DyLight 550	NBP2-37830R
DyLight 594	NBP2-37830DL594
DyLight 650	NBP2-37830C
DyLight 680	NBP2-37830FR
DyLight 755	NBP2-37830IR
FITC	NBP2-37830F
HRP	NBP2-37830H
Janelia Fluor 525	NBP2-37830JF525
Janelia Fluor 549	NBP2-37830JF549
Janelia Fluor 646	NBP2-37830JF646
Janelia Fluor 669	NBP2-37830JF669
PE	NBP2-37830PE
PerCP	NBP2-37830PCP
mFluor Violet 450 SE	NBP2-37830MFV450
mFluor Violet 500 SE	NBP2-37830MFV500
mFluor Violet 610 SE	NBP2-37830MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-37830-0.025mg

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NB120-11312

beta Tubulin Antibody (SAP.4G5)

(2 Publications)

0.1 ml

NB120-11312

Immunohistochemistry-Paraffin: beta Tubulin Antibody (SAP.4G5) [NB120-11312] - Staining of formalin-fixed, paraffin-embedded human testis sections with Monoclonal Anti-beta-Tubulin I, Clone: SAP.4G5 using biotin/ExtrAvidin(R)-Peroxidase.

Immunohistochemistry-Paraffin: beta Tubulin Antibody (SAP.4G5) [NB120-11312] - Staining of formalin-fixed, paraffin-embedded human fallopian tube sections with Monoclonal Anti-beta-Tubulin I, Clone: SAP.4G5 using biotin/ExtrAvidin(R)-Peroxidase.

Characterization of p2TK2-SW2-Tet-J-E-hctB-flag.A) Schematic of the Te-riboJ-E-hctB-flag construct. HctB expression is controlled by an aTc inducible promoter, riboJ insulator and the riboE riboswitch. B) Anti-flag western blot of Cos-7 cells infected with L2-Tet-J-E-hctB-flag comparing expression of theophylline treated and untreated cultures. Cells were induced with 0.5 mM theophylline, 30ng/ml aTc, both aTc and theophylline or vehicle only at 16 hpi and proteins were harvested at 30 hpi. HctB-flag expression was only detected in the samples induced with both aTc and theophylline. C) Confocal micrographs of Cos-7 cells infected with L2-Tet-riboJ-E-hctB-flag, induced with 0.5 mM theophylline, 30ng/ml aTc, both aTc and theophylline or vehicle only at 16 hpi and fixed and stained with DAPI (blue) for confocal microscopy at 30 hpi. The flag tag was stained with a primary antibody to the flag and an alexa 488 anti-mouse secondary antibody (green). Size bar = 10 μm. D) Production of infectious progeny was determined using a reinfection assay. Cos-7 cells were infected with L2-Tet-J-E-hctB-flag and the production of infectious progeny was determined at 48 hpi after induction with 0.5 mM theophylline, 30ng/ml aTc, both aTc and theophylline or vehicle only at 16 hpi. Asterisks denote p-values < 0.05. Error bars = SEM. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35085261), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Characterization of p2TK2-SW2-nprom-E-pgp4-flag.A) Schematic of the nprom-E-pgp4-flag construct consisting of the native pgp4 promoter, the riboE riboswitch (rsE), and the pgp4 ORF with an inframe 3x flag tag. B) Anti-flag western blot of Cos-7 cells infected with L2-nprom-E-pgp4-flag comparing expression of theophylline treated and untreated cultures. Cells were induced or not with 0.5mM theophylline at 16 hpi and proteins were harvested at 30 hpi. A beta -tubulin I western blot served as a loading control. C) Confocal micrographs of Cos-7 cells infected with L2-nprom-E-pgp4-flag, induced or not with 0.5 mM theophylline at 16 hpi and fixed and stained with DAPI to detect DNA. The flag tag was detected using a primary antibody to the tag and an alexa 488 anti-mouse secondary antibody (green). Size bar = 10 μm. D) Cos-7 cells were infected with L2-nprom-E-pgp4-flag and the production of infectious progeny was determined at 48 hpi after 0.5 mM theophylline induction or vehicle only. E) Iodine staining of glycogen in the inclusion of Cos-7 cells infected with L2-nprom-E-pgp4-flag after 0.5 mM theophylline induction at 16 hpi or vehicle only. Arrows indicate the location of the chlamydial inclusions. Asterisk denotes p-value < 0.05. Error bars = SEM. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35085261), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Bv, Ca, Ch, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Xp

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NB120-7792

beta Tubulin Antibody (TU-06) - BSA Free

(1 Review)

(2 Publications)

0.1 mg

NB120-7792

Reactivity:

Hu, Mu, Rt, Po, At, Ch, Eu, Fi, Pl

Species Glossary

Reactivity: Hu, Mu, Rt, Po, At, Ch, Eu, Fi, Pl

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-81064

beta Tubulin Antibody (S11B) - Chimeric - Azide and BSA Free

0.2 mg

NBP2-81064

Immunohistochemistry-Paraffin: beta Tubulin Antibody (S11B) [NBP2-81064] - Immunohistochemical staining of human cerebral cortex tissue using [NBP2-81064]. Anti-Beta Tubulin staining of paraffin embedded human cerebral cortex tissue using the rabbit-chimeric version of S11B [NBP2-81064]. Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-81064 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of neuronal cell bodies and their processes may be observed. Recommended concentration, 2-4 ug/ml.

Flow Cytometry: beta Tubulin Antibody (S11B) [NBP2-81064] - Flow-cytometry using the anti-Beta Tubulin (S11B) [NBP2-81064]. MCF-7 (A) and HeLa (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of S11B (NBP2-81064, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-81064AF350
Alexa Fluor 405	NBP2-81064AF405
Alexa Fluor 488	NBP2-81064AF488
Alexa Fluor 532	NBP2-81064AF532
Alexa Fluor 594	NBP2-81064AF594
Alexa Fluor 647	NBP2-81064AF647
Alexa Fluor 700	NBP2-81064AF700
Alexa Fluor 750	NBP2-81064AF750
Alexa Fluor Plus 405	NBP2-81064AFP405
Alexa Fluor Plus 488	NBP2-81064AFP488
Alexa Fluor Plus 555	NBP2-81064AFP555
Alexa Fluor Plus 594	NBP2-81064AFP594
Alexa Fluor Plus 647	NBP2-81064AFP647
Alexa Fluor Plus 680	NBP2-81064AFP680
Allophycocyanin	NBP2-81064APC
Biotin	NBP2-81064B
CoraFluor 1	NBP2-81064CL1
DyLight 350	NBP2-81064UV
DyLight 405	NBP2-81064V
DyLight 488	NBP2-81064G
DyLight 550	NBP2-81064R
DyLight 594	NBP2-81064DL594
DyLight 650	NBP2-81064C
DyLight 680	NBP2-81064FR
DyLight 755	NBP2-81064IR
FITC	NBP2-81064F
HRP	NBP2-81064H
Janelia Fluor 525	NBP2-81064JF525
Janelia Fluor 549	NBP2-81064JF549
Janelia Fluor 646	NBP2-81064JF646
Janelia Fluor 669	NBP2-81064JF669
PE	NBP2-81064PE
PerCP	NBP2-81064PCP
mFluor Violet 450 SE	NBP2-81064MFV450
mFluor Violet 500 SE	NBP2-81064MFV500
mFluor Violet 610 SE	NBP2-81064MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-81064-0.025mg

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NBP3-15439

beta Tubulin Antibody (4E9G8)

100 ul

NBP3-15439

Western Blot: beta Tubulin Antibody (4E9G8) [NBP3-15439] - Analysis of various lysates using beta -Tubulin Rabbit mAb at 1:5000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 20s.

Western Blot: beta Tubulin Antibody (4E9G8) [NBP3-15439] - Analysis of lysates from 293F cells, using beta -Tubulin Rabbit mAb at 1:1000-1:30000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry-Paraffin: beta Tubulin Antibody (4E9G8) [NBP3-15439] -Analysis of paraffin-embedded Rat kidney tissue using beta -Tubulin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Western Blot: beta Tubulin Antibody (4E9G8) [beta Tubulin] - Western blot analysis of various lysates using beta Tubulin Rabbit mAb at 1:5000 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 20s.

Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (4E9G8) [beta Tubulin] - Immunofluorescence analysis of U2OS cells using beta Tubulin Rabbit mAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation: beta Tubulin Antibody (4E9G8) [beta Tubulin] - Immunoprecipitation analysis of 300 ug extracts of 293T cells using 3 ug beta Tubulin antibody . Western blot was performed from the immunoprecipitate using beta Tubulin antibody at a dilution of 1:1000.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NB600-1514

beta Tubulin Antibody (D66)

(3 Publications)

0.1 ml

NB600-1514

Western Blot: beta Tubulin Antibody (D66) [NB600-1514] - beta Tubulin antibody Total cell extract of PC-12 was separated on SDS-PAGE. The antibody was developed with Goat Anti-Mouse IgG, alkaline phosphatase conjugate and a chemiluminescent substrate. Lanes 1. 0.5 ug/mL antibody 2. 1 ug/mL antibody

beta-Tubulin-Antibody-D66-Western-Blot-NB600-1514-img0002.jpg

Reactivity:

Hu, Mu, Rt, Bv, Ca, Ch, Pm, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Pm, Rb

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-15584

beta Tubulin Antibody

0.1 ml

NBP2-15584

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NBP2-15584] - HeLa xenograft, using beta Tubulin antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

Western Blot: beta Tubulin Antibody [NBP2-15584] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:1000.

Western Blot: beta Tubulin Antibody [NBP2-15584] - Sample (30 ug of whole cell lysate) A: NIH-3T3, B: JC, C: BCL-1, D: C2C12, E: Raw264. 7 10% SDS PAGE NBP2-15584 diluted at 1:1000 .

Reactivity:

Hu, Mu, Po, Bv, Ch, Pm, RM, Xp

Species Glossary

Reactivity: Hu, Mu, Po, Bv, Ch, Pm, RM, Xp

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-32081

beta Tubulin Antibody (A1-A4-R)

100 ul

NBP3-32081

Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (A1-A4-R) [NBP3-32081] - Immunocytochemistry analysis of HeLa cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (NBP3-32081) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (NBP3-32081) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow Cytometry: beta Tubulin Antibody (A1-A4-R) [NBP3-32081] - Flow cytometric analysis of HeLa cells labeling beta Tubulin.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32081, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-50047

beta Tubulin Antibody (4E4) - BSA Free

(2 Publications)

0.1 ml

NBP2-50047

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-50047AF350
Alexa Fluor 405	NBP2-50047AF405
Alexa Fluor 488	NBP2-50047AF488
Alexa Fluor 532	NBP2-50047AF532
Alexa Fluor 594	NBP2-50047AF594
Alexa Fluor 647	NBP2-50047AF647
Alexa Fluor 700	NBP2-50047AF700
Alexa Fluor 750	NBP2-50047AF750
Alexa Fluor Plus 405	NBP2-50047AFP405
Alexa Fluor Plus 488	NBP2-50047AFP488
Alexa Fluor Plus 555	NBP2-50047AFP555
Alexa Fluor Plus 594	NBP2-50047AFP594
Alexa Fluor Plus 647	NBP2-50047AFP647
Alexa Fluor Plus 680	NBP2-50047AFP680
Allophycocyanin	NBP2-50047APC
Biotin	NBP2-50047B
CoraFluor 1	NBP2-50047CL1
DyLight 350	NBP2-50047UV
DyLight 405	NBP2-50047V
DyLight 488	NBP2-50047G
DyLight 550	NBP2-50047R
DyLight 594	NBP2-50047DL594
DyLight 650	NBP2-50047C
DyLight 680	NBP2-50047FR
DyLight 755	NBP2-50047IR
FITC	NBP2-50047F
HRP	NBP2-50047H
Janelia Fluor 525	NBP2-50047JF525
Janelia Fluor 549	NBP2-50047JF549
Janelia Fluor 646	NBP2-50047JF646
Janelia Fluor 669	NBP2-50047JF669
PE	NBP2-50047PE
PerCP	NBP2-50047PCP
mFluor Violet 450 SE	NBP2-50047MFV450
mFluor Violet 500 SE	NBP2-50047MFV500
mFluor Violet 610 SE	NBP2-50047MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-50047SS

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

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beta Tubulin Antibody (4E9G8)

beta Tubulin Antibody (D66)

beta Tubulin Antibody

beta Tubulin Antibody (A1-A4-R)

beta Tubulin Antibody (4E4) - BSA Free