ZNF445 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ZNF445 Antibody [NBP1-80598] - Immunofluorescent staining of human cell line SiHa shows localization to nucleus, cytosol & mitochondria.

Immunohistochemistry-Paraffin: ZNF445 Antibody [NBP1-80598] - Staining of human kidney shows strong granular cytoplasmic positivity in tubule cells.

ChIP-Exo-Seq composite graph for Anti-ZNF445 (NBP1-80598) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• Reactivity: Hu
• Applications: ICC/IF, IHC, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ZNF445 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ZNF445 Antibody - BSA Free (NBP1-80598) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-ZNF445 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: QPKGNPVAAPTGDDLQSKTNKFILNQEPLEEAETLAVSSGCPATSVSEGIGLRESFQQKSRQKDQCENPIQVRVKKEETNFSHRTGKDSEVSGSNSLDLKHVTYLRVSGRKESLKHGCGKHFRMSSHHYDYKKYGK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZNF445
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation-exo-Seq
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ZNF445 Protein (NBP1-80598PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ZNF445 Antibody - BSA Free

• zinc finger protein 168
• zinc finger protein 445
• zinc finger protein with KRAB and SCAN domains 15
• ZKSCAN15
• ZNF168

Background

ZNF445 may be involved in transcriptional regulation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZNF445 Antibody (NBP1-80598)(1)

Publication using NBP1-80598

• Gratuze M, Schlachetzki JCM, D'Oliveira Albanus R et al. TREM2-independent microgliosis promotes tau-mediated neurodegeneration in the presence of ApoE4 Neuron 2022-11-04 [PMID: 36368315] (IHC-Fr, Mouse)
  
  Details:
  Dilution used in WB 1:1000

Bioinformatics

• Gene Symbol: ZNF445
• Entrez:
  • 353274(Human)
• Uniprot:
  • P59923(Human)