ZNF397 Antibody (OTI1F3) - Azide and BSA Free

Western Blot: ZNF397 Antibody (OTI1F3) - Azide and BSA Free [NBP2-74950] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF397 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF397.

Immunocytochemistry/Immunofluorescence: ZNF397 Antibody (OTI1F3) - Azide and BSA Free [NBP2-74950] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ZNF397.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF
• Clone: OTI1F3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ZNF397 Antibody (OTI1F3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ZNF397 Antibody (OTI1F3) - Azide and BSA Free (NBP2-01324) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ZNF397(NP_115723) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZNF397
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 30.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ZNF397 Antibody (OTI1F3) - Azide and BSA Free

• KIAA1588
• KRAB-containing zinc finger protein 317
• zinc finger protein 317

Background

The ZNF397 gene encodes a protein with a N-terminal SCAN domain, and the longer isoform contains nine C2H2-type zinc fingerrepeats in the C-terminal domain. The protein localizes to centromeres during interphase and early prophase, anddifferent isoforms can rep

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZNF397