Zinc finger protein 639 Antibody (PCRP-ZNF639-2B2)

Immunocytochemistry/Immunofluorescence: Zinc finger protein 639 Antibody (PCRP-ZNF639-2B2) [NBP3-13995] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Zinc finger protein 639 Antibody (PCRP-ZNF639-2B2) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Flow Cytometry: Zinc finger protein 639 Antibody (PCRP-ZNF639-2B2) [NBP3-13995] - Flow cytometric analysis of PFA-fixed HeLa cells. Zinc finger protein 639 antibody (PCRP-ZNF639-2B2) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Protein Array: Zinc finger protein 639 Antibody (PCRP-ZNF639-2B2) [NBP3-13995] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Zinc finger protein 639 Antibody (PCRP-ZNF639-2B2).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IP, MA
• Clone: PCRP-ZNF639-2B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Zinc finger protein 639 Antibody (PCRP-ZNF639-2B2) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14131)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant human fragment (aa 406-485) of Zinc finger protein 639 (Uniprot: Q9UID6)
• Localization: Nucleus
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZNF639
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA: For coating, order antibody without BSA
  
  Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
• Theoretical MW: 56.05 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react in Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Zinc finger protein 639 Antibody (PCRP-ZNF639-2B2)

• ANC_2H01
• ZASC1ANC-2H01
• zinc finger amplified in esophageal squamous cell carcinomas 1
• zinc finger protein 639
• Zinc finger protein ANC_2H01
• Zinc finger protein ZASC1,6230400O18Rik

Background

Zinc finger protein 639 binds DNA and may function as a transcriptional repressor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZNF639
• Uniprot:
  • Q9UID6(Human)