WDR58 Antibody (1F6) - Azide and BSA Free

Western Blot: WDR58 Antibody (1F6) [H00079228-M01] - WDR58 monoclonal antibody (M01), clone 1F6 Analysis of WDR58 expression in A-431.

Immunohistochemistry-Paraffin: WDR58 Antibody (1F6) [H00079228-M01] - Analysis of monoclonal antibody to WDR58 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.

Western Blot: WDR58 Antibody (1F6) [H00079228-M01] - WDR58 monoclonal antibody (M01), clone 1F6. Analysis of WDR58 expression in PC-12.

Western Blot: WDR58 Antibody (1F6) [H00079228-M01] - Analysis of THOC6 expression in transfected 293T cell line by THOC6 monoclonal antibody (M01), clone 1F6.Lane 1: THOC6 transfected lysate (Predicted MW: 37.5 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: WDR58 Antibody (1F6) [H00079228-M01] - Detection limit for recombinant GST tagged WDR58 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, IHC
• Clone: 1F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

WDR58 Antibody (1F6) - Azide and BSA Free Summary

• Immunogen: WDR58 (NP_077315, 71 a.a. ~ 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TFQAHDGPVYSMVSTDRHLLSAGDGEVKAWLWAEMLKKGCKELWRRQPPYRTSLEVPEINALLLVPKENSLILAGGDCQLHTMDLETGTFTRVLRGHTDYIHCLALRERS
• Specificity: WDR58 - WD repeat domain 58
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: THOC6
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for WDR58 Antibody (1F6) - Azide and BSA Free

• fSAP35WDR58
• Functional spliceosome-associated protein 35
• MGC2655
• THO complex 6 homolog (Drosophila)
• THO complex subunit 6 homolog
• WD repeat domain 58
• WD repeat-containing protein 58

Background

WDR58 is a component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with splicedmRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Bindsto mRNA upstream of the exon-ju

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WDR58 Antibody (H00079228-M01)(2)

Publications using H00079228-M01

• Katahira J, Okuzaki D, Inoue H et al. Human TREX component Thoc5 affects alternative polyadenylation site choice by recruiting mammalian cleavage factor I. Nucleic Acids Res. 2013-05-17 [PMID: 23685434]
• Katahira J, Inoue H, Hurt E, Yoneda Y. Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA. EMBO J. 2009-03-04 [PMID: 19165146]

Bioinformatics

• Gene Symbol: THOC6
• Entrez:
  • 79228(Human)
• Uniprot:
  • NP_077315(Human)