WDR45 Antibody - BSA Free

Western Blot: WDR45 Antibody [NBP3-18255] - Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 40 kDa WDR45 protein using Rabbit Anti-WDR45 Polyclonal Antibody (NBP3-18255). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WDR45 Polyclonal Antibody (NBP3-18255) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 40 kDa.

Immunocytochemistry/Immunofluorescence: WDR45 Antibody [NBP3-18255] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDR45 Polyclonal Antibody (NBP3-18255). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDR45 Polyclonal Antibody (NBP3-18255) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) WDR45 Antibody (D) Composite.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

WDR45 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit WDR45 Antibody - BSA Free (NBP3-18255) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide from the C-terminal of Human WDR45(aa. 312-322)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WDR45
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Application Notes: Optimal dilutions for assays should be determined by the user.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for WDR45 Antibody - BSA Free

• JM5
• WD repeat domain 45
• WD repeat domain phosphoinositide-interacting protein 4
• WD repeat-containing protein 45
• WD45 repeat protein interacting with phosphoinositides 4
• WDRX1
• WDRXI4
• WIPI-4
• X-linked 1

Background

WDR45 encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene has a pseudogene at chromosome 4q31.3. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity and full-length nature of some variants have not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WDR45