Human VIPR1/VPAC1 ELISA Kit (Colorimetric)

ELISA: Human VIPR1/VPAC1 ELISA Kit (Colorimetric) [NBP2-82391] - Samples were spiked with high concentrations of Human VIPR1/VPAC1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human VIPR1/VPAC1 ELISA Kit (Colorimetric) [NBP2-82391] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL

Human VIPR1/VPAC1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human VIPR1/VPAC1 in samples. No Significant cross-reactivity or interference between Human VIPR1/VPAC1 and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: CV% < 5.11
• Intra-Assay: CV% < 4.94
• Spike Recovery: 95-111%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: VIPR1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human VIPR1/VPAC1 ELISA Kit (Colorimetric)

• FLJ41949
• HVR1
• II
• PACAP type II receptor
• PACAP-R2
• RDC1
• type II
• V1RG
• vasoactive intestinal peptide receptor 1
• vasoactive intestinal polypeptide receptor 1
• VIP R1
• VIP receptor, type I
• VIPR
• VIPR1
• VPAC1
• VPAC1R

Background

The Vasoactive Intestinal Polypeptide Receptor 1 (VPAC1) is a member of a family of three G-protein coupled receptors which mediate VIP (vasoactive intestinal peptide; a small neuropeptide) and PACAP (pituitary adenlyate cyclase activating peptide) function in a variety of tissues. Together, the VPAC1 and VPAC2 receptors compose a subtype of the VIP receptor. Expression of the VIP and PACAP receptors has been linked to the presence of human tumors. Vasoactive intestinal peptide is involved in smooth muscle relaxation, exocrine and endocrine secretion, and water and ion flux in lung and intestinal epithelia. Its actions are effected through integral membrane receptors associated with a guanine nucleotide binding protein which activates adenylate cyclase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for VIPR1/VPAC1 ELISA Kit (NBP2-82391). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: VIPR1