USP22 Antibody (JU63-27)

Western Blot: USP22 Antibody (JU63-27) [NBP2-75702] - Analysis of USP22 on Hela (1) and HepG2 (2) cell lysate using anti-USP22 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: USP22 Antibody (JU63-27) [NBP2-75702] - Staining USP22 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow Cytometry: USP22 Antibody (JU63-27) [NBP2-75702] - Analysis of Hela cells with USP22 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: USP22 Antibody (JU63-27) [NBP2-75702] - Staining USP22 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: USP22 Antibody (JU63-27) [NBP2-75702] - Staining USP22 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

USP22 Antibody (JU63-27) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human USP22 aa 321-370 / 525. (SwissProt: Q9UPT9 Human)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: USP22
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:1000-1:5000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for USP22 Antibody (JU63-27)

• Deubiquitinating enzyme 22
• EC 3.1.2.15
• EC 3.4.19.12
• KIAA1063ubiquitin carboxyl-terminal hydrolase 22
• KIAA1064
• ubiquitin specific peptidase 22
• ubiquitin specific peptidase 3-like
• ubiquitin specific protease 22
• Ubiquitin Thioesterase 22
• Ubiquitin thiolesterase 22
• ubiquitin-specific processing protease 22
• Ubiquitin-specific-processing protease 22
• USP22
• USP3L

Background

Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP22