U2AF1L4 Antibody

Western Blot: U2AF1L4 Antibody [NBP1-06996] - Analysis of U2AF1L4 in Human Cerebellum lysate (35ug protein in RIPA buffer) using NBP1-06996 at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

U2AF1L4 Antibody Summary

• Description: Novus Biologicals Goat U2AF1L4 Antibody (NBP1-06996) is a polyclonal antibody validated for use in WB and ELISA. Anti-U2AF1L4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EDGERAVAELSNR corresponding to internal region according to NP_659424.2.
• Epitope: internal region
• Specificity: This antibody is expected to recognise both reported isoforms (NP_001035515.1 and NP_659424.2). No cross-reactivity with U2AF1 expected.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: U2AF1L4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 20 kDa band observed in human brain (cerebellum) and lung lysates (calculated MW of 21.3 kDa band according to NP_001035515.1).
• Control:

  U2AF1L4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for U2AF1L4 Antibody

• FLJ35525
• MGC33901
• splicing factor U2AF 26 kDa subunit
• U2 auxiliary factor 26
• U2 small nuclear RNA auxiliary factor 1-like 3
• U2 small nuclear RNA auxiliary factor 1-like 4
• U2 small nuclear RNA auxiliary factor 1-like protein 3
• U2 small nuclear RNA auxiliary factor 1-like protein 4
• U2(RNU2) small nuclear RNA auxiliary factor 1-like 3
• U2(RNU2) small nuclear RNA auxiliary factor 1-like protein 3
• U2AF1L3
• U2AF1L3V1
• U2AF1-like protein 3
• U2AF1RS3
• U2AF1-RS3
• U2af26

Background

RNA-binding protein that function as a pre-mRNA splicing factor. Plays a critical role in both constitutiveand enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required foraccurate 3'-splice site selection. Acts by enhancing the binding of U2AF2 to weak pyrimidine tracts. Also participatesin the regulation of alternative pre-mRNA splicing. Activates exon 5 skipping of PTPRC during T cell activation; anevent reversed by GFI1. Binds to RNA at the AG dinucleotide at the 3'-splice site

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for U2AF1L4 Antibody (NBP1-06996)(1)

Publication using NBP1-06996

• Chen F, Ji C, Dou T et al. Cloning and characterization of a novel splice variant of human U2AF1L3 gene. DNA Seq 2006-08-01 [PMID: 17312947]

Bioinformatics

• Gene Symbol: U2AF1L4
• Entrez:
  • 199746(Human)
  • 233073(Mouse)
  • 361542(Rat)
• Uniprot:
  • NP_001035515.1(Human)