Tryptase epsilon/BSSP-4/PRSS22 Antibody (246703) [Biotin]

• Reactivity: Mu
• Applications: ELISA(Det)
• Clone: 246703
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Biotin
• Concentration: LYOPH

Tryptase epsilon/BSSP-4/PRSS22 Antibody (246703) [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Tryptase epsilon /BSSP‑4
  Ala33-Ser306
  Accession # Q9ER10
• Specificity: Detects mouse Tryptase epsilon /BSSP‑4 in ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: PRSS22
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Mouse Tryptase epsilon/BSSP-4 Antibody (Catalog # MAB20592)
  ELISA Detection: Mouse Tryptase epsilon/BSSP-4 Biotinylated Antibody (Catalog # BAM20591)
  Standard: Recombinant Mouse Tryptase epsilon/BSSP-4 (Catalog # 2059-SE)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tryptase epsilon/BSSP-4/PRSS22 Antibody (246703) [Biotin]

• BSP-2
• BSSP4
• BSSP-4
• hBSSP-4
• MGC9599
• prosemin
• protease, serine S1 family member 22
• protease, serine, 22
• PRSS22
• serine protease 22
• serine protease 26
• SP001LA
• Tryptase epsilon
• UNQ302/PRO343

Background

Tryptase epsilon , brain specific serine protease 4 (BSSP-4) and brain serine protease 2 (BSP-2) are different names given for the same serine protease that is encoded by the PRSS22 gene (1‑3). Initially identified having brain-specific expression, mouse Tryptase epsilon is preferentially expressed in epithelium-rich tissues such as the lung and eye, which is similar to its human counterpart (3). The mouse protein is synthesized with a signal peptide (amino acid residues 1 to 32), a pro peptide (residues 33 to 49) and a mature chain (residues 50 to 306) corresponding to the serine protease domain.

1. Wong, G.W. et al. (2001) J. Biol. Chem. 276:49169.
2. Davies, B.J. et al. (1998) J. Biol. Chem. 273:23004.
3. Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRSS22
• Uniprot:
  • Q9ER10()