Tryptase alpha/TPS1 Antibody (AA1)

Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (AA1) [NBP1-40202] - Analysis of anti-TPSAB1 / Mast Cell Tryptase antibody with human lung at concentration 5 ug/ml.

Product Discontinued

Tryptase alpha/TPS1 Antibody (AA1) Summary

• Immunogen: Human mast cell tryptase purified from human lung tissue.
• Specificity: Recognizes human mast cell tryptase, both alpha and beta isoforms. The antibody is an excellent marker for mast cells and does not bind to any other cell type in Immunohistochemistry. Species cross-reactivity: feline, canine and monkey.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPSAB1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Tryptase alpha/TPS1 Antibody (AA1)

• TPS1
• TPS2
• TPSAB1
• TPSB1
• tryptase alpha/beta 1

Background

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tryptase alpha/TPS1 Antibody (NBP1-40202)(1)

Publication using NBP1-40202

• Galiazzo G, Giancola F, Stanzani A et al. Localization of cannabinoid receptors CB1, CB2, GPR55, and PPARalpha in the canine gastrointestinal tract. Histochem Cell Biol 2018-06-07 [PMID: 29882158]

Bioinformatics

• Gene Symbol: TPSAB1
• Entrez:
  • 7177(Human)
• Uniprot:
  • Q15661(Human)