Human Troponin I Type 3 (cardiac) ELISA Kit (Colorimetric) Summary
| Specificity |
No cross reactivity to similar molecules (cardiac troponin T and cardiac troponin C). There is slight cross-reactivity to muscle troponin I. |
| Standard Curve Range |
0.38 - 25 ng/ml |
| Sensitivity |
<0.38 ng/ml |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
Low Concentration %CV - 0.4%
Medium Concentration %CV 1 - 1.4%
Medium Concentration %CV 2 - 3.3%
High - 6.1% |
| Intra-Assay |
Low Concentration %CV - 6.0%
Medium Concentration %CV 1 - 5.9%
Medium Concentration %CV 2 - 8.9%
High - 3.7% |
| Spike Recovery |
Recovery on addition is 98.3~104.2 % (mean 100.8%)
Recovery on dilution is 99.3~103.9 % (mean 100.7 %) |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
TNNI3 |
Applications/Dilutions
Reactivity Notes
No cross reactivity to similar molecules (cardiac troponin T and cardiac troponin C). There is slight cross-reactivity to muscle troponin I.
Packaging, Storage & Formulations
Kit Components
|
Components
|
- AV-HRP
- Dilution Buffers
- Microtiter Plate
- Protein Standard
- Secondary Antibody
- Stop Solution
- TMB Substrate
- Washing Buffer
|
Alternate Names for Human Troponin I Type 3 (cardiac) ELISA Kit (Colorimetric)
Background
Equally reactive with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. (In the presence of 5 mM EDTA). Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation. No cross-reaction with skeletal muscle troponin I.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Troponin I Type 3 (cardiac) ELISA Kit (NBP2-62177). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.