Troponin I Type 3 (cardiac) Antibody (M155)

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Product Details

Summary
Reactivity Mu, Rt, CaSpecies Glossary
Applications WB, ELISA, IA, IHC, IHC-P, IP
Clone
M155
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Troponin I Type 3 (cardiac) Antibody (M155) Summary

Immunogen
Free human cardiac troponin and/or native troponin complex.
Epitope
27-43 aa
Specificity
Reacts with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (in the presence of 5 mM EDTA). Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Does not cross-react with skeletal muscle.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNNI3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunoassay
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Cross-reacts with Mouse, Rat and Dog. Expected to cross-react with Human due to sequence identity. May also react with Bovine and Porcine. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for Troponin I Type 3 (cardiac) Antibody (M155)

  • Cardiac troponin I
  • CMD1FF
  • CMD2A
  • CMH7MGC116817
  • cTnI
  • familial hypertrophic cardiomyopathy 7
  • RCM1
  • TNNC1troponin I, cardiac
  • troponin I type 3 (cardiac)
  • troponin I, cardiac muscle

Background

In clinical studies of new drugs, new methods of surgery are carried out on experimental animals. The effects of the new technologies on cardiac function and on cardiac myocyte viability can be important. The majority of the anti-cardiac Troponin I MAbs have wide specificity and recognize not only human cardiac Troponin I but also cardiac Troponin I from other species. Several combinations of MAbs were tested and could be used for quantitative detection of cardiac Troponin I in blood of different species. An assay utilizing MAb M155 as a capture and MAb 19C7 as detection gives equal response with antigens from different animal species. Other MAb pairs, with epitopes located in different parts of troponin molecule, could also be used for the same purpose.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics Tool for Troponin I Type 3 (cardiac) Antibody (NB110-8459)

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Blogs on Troponin I Type 3 (cardiac).

Troponin I Type 3 (TnI, cardiac)
Troponin I is part of a heteromeric thin filament regulatory complex that governs skeletal and cardiac muscle contraction. Troponin I is composed of three subunits: troponin I (TnI), troponin T (TnT), and troponin C (TnC), and each of these components...  Read full blog post.

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Bioinformatics

Gene Symbol TNNI3
Entrez