Triadin Antibody (GE 4.90)

Immunohistochemistry-Frozen: Triadin Antibody (GE 4.90) [NB300-623] - Mouse breast muscle.

Product Discontinued

Triadin Antibody (GE 4.90) Summary

• Immunogen: Purified rabbit fast skeletal muscle triadin.
• Specificity: Triadin (GE 4.90)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRDN
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Frozen 1:50
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Triadin Antibody (GE 4.90)

• dJ166D18.1 (triadin)
• TDN
• triadin
• TRISK

Background

The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR's) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyR's), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, Triadin (MW 95 kDa) transmits electrochemical signals to the SR through direct interaction with both DHPR's and RyR's. Though its exact role in this signaling process is unclear, triadin has been shown to co-localize with both DHPR and RYR at the junctional face of the terminal cisternae.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRDN
• Entrez:
  • 76757(Mouse)
  • 59299(Rat)