TIP60 Overexpression Lysate (Native)

Images

 
Western Blot: TIP60 Overexpression Lysate (Adult Normal) [NBL1-12125] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TIP60.

Product Details

Summary
Applications WB

Order Details

TIP60 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210591 and based on accession number NM_182709. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens K(lysine) acetyltransferase 5 (KAT5), transcript variant 3, mRNA.
Gene
KAT5

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
RIPA buffer

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
52.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for TIP60

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TIP60 Overexpression Lysate (Native)

  • cPLA2
  • ESA1EC 2.3.1.48
  • Histone acetyltransferase HTATIP
  • histone acetyltransferase KAT5
  • HIV-1 Tat interactive protein
  • HIV-1 Tat interactive protein, 60kDa
  • HTATIP1
  • HTATIPcPLA2 interacting protein
  • K(lysine) acetyltransferase 5,60 kDa Tat-interactive protein
  • K-acetyltransferase 5
  • Lysine acetyltransferase 5
  • PLIPTIP
  • Tat interacting protein, 60kDa
  • Tip60
  • TIP60cPLA(2)-interacting protein

Background

The protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]. Transcript Variant: This variant (3), also known as beta, lacks two alternate in-frame segments, compared to variant 1, resulting in a shorter protein (isoform 3), that has a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF4925
Species: Mu
Applications: WB, Simple Western, IHC
NBP2-32569
Species: Hu
Applications: IHC, IHC-P
NBP1-81586
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-43581
Species: Hu
Applications: WB, ICC/IF, IP
NB100-689
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
NB100-92066
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-24593
Species: Hu, Mu, Rt, Bv, Ca, Pm
Applications: WB, IHC-P
NBP1-47842
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
MAB18251
Species: Hu, Mu
Applications: WB, Simple Western
H00008399-D01P
Species: Hu, Rt
Applications: WB, ELISA
NBP1-89522
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-52488
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, IHC, ICC
NBP1-84168
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBL1-12125
Applications: WB

Publications for TIP60 Lysate (NBL1-12125) (0)

There are no publications for TIP60 Lysate (NBL1-12125).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TIP60 Lysate (NBL1-12125) (0)

There are no reviews for TIP60 Lysate (NBL1-12125). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TIP60 Lysate (NBL1-12125) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TIP60 Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for TIP60 Lysate (NBL1-12125)

Discover related pathways, diseases and genes to TIP60 Lysate (NBL1-12125). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TIP60 Lysate (NBL1-12125)

Discover more about diseases related to TIP60 Lysate (NBL1-12125).
 

Pathways for TIP60 Lysate (NBL1-12125)

View related products by pathway.

PTMs for TIP60 Lysate (NBL1-12125)

Learn more about PTMs related to TIP60 Lysate (NBL1-12125).
 

Research Areas for TIP60 Lysate (NBL1-12125)

Find related products by research area.

Blogs on TIP60

There are no specific blogs for TIP60, but you can read our latest blog posts.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol KAT5

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBL1-12125 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

TIP60 Recombinant Protein
H00010524-P01-2ug
TIP60 Antibody
NBP2-24613
TIP60 Antibody
NBP2-24613
TIP60 Antibody
NBP2-24613
COX-2 Antibody
NB100-689
COX-2 Antibody
NB100-689
COX-2 Antibody
NB100-689