TIP60 Antibody (3F9)

Western Blot: TIP60 Antibody (3F9G7) [NB110-87055] - Analysis using Tip60 mouse mAb against truncated Tip60 recombinant protein.

Immunohistochemistry-Paraffin: TIP60 Antibody (3F9G7) [NB110-87055] - Analysis of human liver carcinoma (A), recturn carcinoma (B), normal medulla tissue (C) and normal interbrain tissues (D), showing nuclear localization using Tip60 mouse mAb with DAB staining.

Product Discontinued

TIP60 Antibody (3F9) Summary

• Immunogen: Purified recombinant fragment of human TIP60 expressed in E. Coli.
• Specificity: TIP60 (3F9)
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: KAT5
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is useful for: Western Blot: 1:500- 1: 2,000 IHC-P: 1:500- 1:2000 ELISA: Proposed dilution 1:10,000.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  TIP60 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for TIP60 Antibody (3F9)

• cPLA2
• ESA1EC 2.3.1.48
• Histone acetyltransferase HTATIP
• histone acetyltransferase KAT5
• HIV-1 Tat interactive protein
• HIV-1 Tat interactive protein, 60kDa
• HTATIP
• HTATIP1
• HTATIPcPLA2 interacting protein
• K(lysine) acetyltransferase 5,60 kDa Tat-interactive protein
• K-acetyltransferase 5
• KAT5
• Lysine acetyltransferase 5
• PLIPTIP
• Tat interacting protein, 60kDa
• TIP60
• TIP60cPLA(2)-interacting protein

Background

HTATIP (HIV-1 Tat interacting protein TIP60, about 60kDa) belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. In addition to the growing number of post-translational histone modifications regulating chromatin structure, cells can also exchange canonical histones with variant histones that can directly or indirectly modulate chromatin structure. There are five major variants of histone H2A: canonical H2A (most abundant), H2A.X, MacroH2A, H2ABbd and H2A.Z. Histone H2A.Z, the most conserved variant across species, functions as both a positive and negative regulator of transcription and is important for chromosome stability. Several homologous protein complexes, such as SWR-C, TIP60 and SRCAP (mammals), have been shown to catalyze the ATP-dependent exchange of H2A.Z for H2A in the nucleosome. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KAT5
• Entrez:
  • 10524(Human)
• Uniprot:
  • Q92993(Human)