THAP10 Antibody

Western Blot: THAP10 Antibody [NBP1-86226] - Analysis in control (vector only transfected HEK293T lysate) and THAP10 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: THAP10 Antibody [NBP1-86226] - THAP10 localizes in the nuclei and inhibits proliferation but promotes differentiation of t(8;21) AML cells ANuclear localization of THAP10 in NIH3T3 cells. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/10.15252/emmm.201607180), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: THAP10 Antibody [NBP1-86226] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Immunocytochemistry/Immunofluorescence: THAP10 Antibody [NBP1-86226] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

THAP10 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: HSEAAPGPVSCTRPRAGKQAAASQITCENELVQTQPHADNPSNTVTSVPTHCEEGPVHKSTQISLKRPRHRSVGIQAKVKAFGKRLCNATTQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: THAP10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:100 - 1:250
• Application Notes: ICC/IF reported in scientific literature (PMID: 28539478). For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  THAP10 Protein (NBP1-86226PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for THAP10 Antibody

• THAP domain containing 10
• THAP domain-containing protein 10

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for THAP10 Antibody (NBP1-86226)(1)

Publication using NBP1-86226

• Li Y, Ning Q, Shi J et al. A novel epigenetic AML1-ETO/THAP10/miR-383 mini-circuitry contributes to t(8;21) leukaemogenesis.. EMBO Mol Med 2017-05-24 [PMID: 28539478] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: THAP10