TAF6L 293T Cell Transient Overexpression Lysate

TAF6L transfected lysate.

Lane 1: TAF6L transfected lysate (50.6 KDa) Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB

TAF6L 293T Cell Transient Overexpression Lysate Summary

• Description: Plasmid: pCMV-TAF6L full-length
• Specificity: TAF6L 293T Cell Transient Overexpression Lysate(Denatured)
• Gene: TAF6L

Applications/Dilutions

• Dilutions:
  • Western Blot
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

• Type: 293T Cell Transient Overexpression
• Protein State: Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-TAF6L antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TAF6L 293T Cell Transient Overexpression Lysate

• FLJ11136
• p300/CBP-associated factor (PCAF)-associated factor 65
• PAF65-alpha
• PAF65AMGC4288
• PCAF-associated factor 65 alpha
• PCAF-associated factor 65-alpha
• TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDasubunit 6L
• TAF6-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associatedfactor, 65 kD
• TAF6-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associatedfactor, 65kDa

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a protein that is a component of the PCAF histone acetylase complex and structurally similar to one of the histone-like TAFs, TAF6. The PCAF histone acetylase complex, which is composed of more than 20 polypeptides some of which are TAFs, is required for myogenic transcription and differentiation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: TAF6L