Syntaxin 5 Antibody [Alexa Fluor® 532]

• Reactivity: Hu, Rt
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 532

Syntaxin 5 Antibody [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human Syntaxin 5 isoform 2
  Met55-Thr300
  Accession # Q13190
• Specificity: Detects human Syntaxin 5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) Syntaxin 7, rhSyntaxin 8, and rhSytaxin 12 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Syntaxin 5 Antibody [Alexa Fluor® 532]

• SED5
• STX5
• STX5ASED5
• Syntaxin 5
• syntaxin 5A
• syntaxin-5

Background

Syntaxin 5 (STX5) is a predicted 41‑42 kDa member of the syntaxin family of proteins. It is a t‑SNARE that is widely expressed (but not in striated muscle), and embedded in either the cis‑Golgi network (a 37 kDa form) or the ER (the 46 kDa form). STX5 is involved in the regeneration and maintenance of the Golgi apparatus after mitosis. Human STX5 is a type IV single‑pass transmembrane protein (very long cytoplasmic N‑terminus) that is 355 amino acids (aa) in length. It contains an ER retrieval signal (Arg4Lys5Arg6), a t‑SNARE domain (aa 263‑325) with a coiled‑coil region (aa 287‑318), and a C‑terminal transmembrane sequence. The 35 kDa short form exhibits an alternate start site at Met55. Two other potential splice variants show either the Met55, or a Met97 start site coupled to a 19 aa substitution for aa 303‑355. Over aa 55‑300, human STX5 shares 94% aa identity with mouse STX5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.