Stomatin Antibody

Western Blot: Stomatin Antibody [NBP2-26195] - Staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Stomatin Antibody [NBP2-26195] - Staining of Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Stomatin Antibody Summary

• Description: Novus Biologicals Goat Stomatin Antibody (NBP2-26195) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Stomatin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence EKRHTRDSEAQR-C corresponding to N-Terminus according to NP_004090.4, NP_937837.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_004090.4; NP_937837.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: STOM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 30 kDa band observed in human erythrocytes, spleen, bone marrow and lung lysates (calculated MW of 31.7 kDa band according to NP_004090.4). IHC-P: Human liver shows membrane staining of hepatocytes and of sinusoidal lining cells.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Stomatin Antibody

• BND7EPB7
• EPB72erythrocyte band 7 integral membrane protein
• erythrocyte membrane protein band 7.2 (stomatin)
• Protein 7.2b
• Stomatin

Background

Thought to regulate cation conductance. May regulate ACCN1 and ACCN3 gating

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Stomatin Antibody (NBP2-26195)(1)

Publication using NBP2-26195

• Mrowczynska L, Salzer U, Perutkova S et al. Echinophilic proteins stomatin, sorcin, and synexin locate outside gangliosideM1 (GM1) patches in the erythrocyte membrane. Biochem Biophys Res Commun 2010-10-22 [PMID: 20858460]

Bioinformatics

• Gene Symbol: STOM
• Entrez:
  • 2040(Human)