Stomatin Antibody (33F5) - BSA Free

Western Blot: Stomatin Antibody (33F5) [NBP2-42660] - The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STOM antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STOM antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: STOM Transfected 293T cell lysate

Immunocytochemistry/Immunofluorescence: Stomatin Antibody (33F5) [NBP2-42660] - Analysis of STOM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human STOM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 33F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Stomatin Antibody (33F5) - BSA Free Summary

• Immunogen: Recombinant human Stomatin (55-288aa) purified from E. coli
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: STOM
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Stomatin Antibody (33F5) - BSA Free

• BND7EPB7
• EPB72erythrocyte band 7 integral membrane protein
• erythrocyte membrane protein band 7.2 (stomatin)
• Protein 7.2b
• Stomatin

Background

Thought to regulate cation conductance. May regulate ACCN1 and ACCN3 gating

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STOM
• Uniprot:
  • NP_004090(Human)