![Western Blot: Stomatin Antibody (33F5) [NBP2-42660] - The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STOM antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STOM antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: STOM Transfected 293T cell lysate](http://images.novusbio.com/fullsize2/Stomatin-Antibody-(33F5)-Western-Blot-NBP2-42660-img0001.jpg "Western Blot: Stomatin Antibody (33F5) [NBP2-42660] - The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STOM antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STOM antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: STOM Transfected 293T cell lysate")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 33F5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant human Stomatin (55-288aa) purified from E. coli |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | STOM |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 10% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Western Blot: SLP-2 Antibody (OTI1F5) [NBP2-45464] - SLP-2 Antibody (1F5) [NBP2-45464] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLP-2.](http://images.novusbio.com/images2/SLP-2-Antibody-(OTI1F5)-Western-Blot-NBP2-45464-img0015.jpg)
![Immunohistochemistry: SLP-2 Antibody (1F5) [NBP2-45464] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/images2/SLP-2-Antibody-(1F5)-Immunohistochemistry-NBP2-45464-img0001.jpg)
![Immunohistochemistry: SLP-2 Antibody (1F5) [NBP2-45464] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/images2/SLP-2-Antibody-(1F5)-Immunohistochemistry-NBP2-45464-img0002.jpg)
![Western Blot: Glut1 Antibody [NB110-39113] - Glut1 in human brain lysate (55 kDa). Antibody at 1:500. Western blot image submitted by a verified customer review.](http://images.novusbio.com/images2/Glut1-Antibody-Western-Blot-NB110-39113-img0016.jpg)
![Flow Cytometry: Glut1 Antibody [NB110-39113] - An intracellular stain was performed on HepG2 with NB110-39113 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/images2/Glut1-Antibody-Flow-Cytometry-NB110-39113-img0018.jpg)
![Western Blot: Glut1 Antibody [NB110-39113] - PrP-mediated increase in IC iron downregulates glucose transporters in the brain, neuroretina, and the liver: A similar evaluation of retinal lysates shows upregulation of Glut1 in PrP-/- relative to Tg40 PrP samples (lanes 1 & 3). Iron overloading downregulates Glut1 in Tg40 PrP samples relative to untreated controls, but has minimal effect on similarly treated PrP-/- samples (lanes 2 & 4). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-018-24786-1) licensed under a CC-BY licence.](http://images.novusbio.com/images2/Glut1-Antibody-Western-Blot-NB110-39113-img0019.jpg)
![Western Blot: Stomatin-like protein 1 Antibody [NBP1-92455] - Analysis in control (vector only transfected HEK293T lysate) and STOML1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/Stomatin-like-protein-1-Antibody-Western-Blot-NBP1-92455-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Stomatin-like protein 1 Antibody [NBP1-92455] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.](http://images.novusbio.com/images2/Stomatin-like-protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92455-img0006.jpg)
![Immunohistochemistry-Paraffin: Stomatin-like protein 1 Antibody [NBP1-92455] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.](http://images.novusbio.com/images2/Stomatin-like-protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-92455-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: STOML3 Antibody [NBP1-84832] - Staining of human cell line AF22 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/images2/STOML3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84832-img0001.jpg)
![Western Blot: STOML3 Antibody [NBP1-84832] - Analysis in control (vector only transfected HEK293T lysate) and STOML3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/STOML3-Antibody-Western-Blot-NBP1-84832-img0002.jpg)
![Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/images2/Caveolin-1-Antibody-(7C8)-Flow-Cytometry-NB100-615-img0011.jpg)
![Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.](http://images.novusbio.com/images2/Caveolin-1-Antibody-(7C8)-Flow-Cytometry-NB100-615-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Caveolin-1 [7C8] conjugated to Alexa Fluor 488 (NB100-615AF488) at 20 ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/Caveolin-1-Antibody-(7C8)-Immunocytochemistry-Immunofluorescence-NB100-615-img0013.jpg)
![Western Blot: RUVBL1 Antibody [NBP2-20245] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody diluted at 1:1000.](http://images.novusbio.com/images2/RUVBL1-Antibody-Western-Blot-NBP2-20245-img0013.jpg)
![Western Blot: RUVBL1 Antibody [NBP2-20245] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody diluted at 1:2500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/images2/RUVBL1-Antibody-Western-Blot-NBP2-20245-img0014.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Human colon carcinoma, using RUVBL1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/images2/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP2-20245-img0002.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for Stomatin Antibody (NBP2-42660)Discover more about diseases related to Stomatin Antibody (NBP2-42660).
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PTMs for Stomatin Antibody (NBP2-42660)Learn more about PTMs related to Stomatin Antibody (NBP2-42660).
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![Immunocytochemistry/Immunofluorescence: Stomatin Antibody (33F5) [NBP2-42660] - Analysis of STOM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human STOM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize2/Stomatin-Antibody-(33F5)-Immunocytochemistry-Immunofluorescence-NBP2-42660-img0002.jpg "Immunocytochemistry/Immunofluorescence: Stomatin Antibody (33F5) [NBP2-42660] - Analysis of STOM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human STOM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).")
![Simple Western: Flotillin-1 Antibody [NBP1-79022] - Simple Western lane view shows lysates of Exosome Standards (A549 cell line) (NBP3-11645), exosome standards (HT29 cell line) (NBP3-11685), and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL), loaded at 0.2 mg/mL. A specific band was detected for Flotillin-1 at approximately 51 kDa (as indicated) using 1:250 ug/mL of Flotillin-1 antibody (NBP1-79022). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/images2/Flotillin-1-Antibody-Simple-Western-NBP1-79022-img0003.jpg)
![Western Blot: Flotillin-1 Antibody [NBP1-79022] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope.](http://images.novusbio.com/images2/Flotillin-1-Antibody-Western-Blot-NBP1-79022-img0002.jpg)
![Western Blot: Flotillin 2 Antibody [NBP1-30881] - 293T whole cell extracts and 293T exosome extract (2.6 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 2 antibody [C3], C-term (NBP1-30881). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/images2/Flotillin-2-Antibody-Western-Blot-NBP1-30881-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Flotillin 2 Antibody [NBP1-30881] - Analysis of paraformaldehyde-fixed HeLaS3, using antibody at 1:100 dilution.](http://images.novusbio.com/images2/Flotillin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30881-img0004.jpg)
![Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP1-30881] - Human lung cancer, using Flotillin-2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/images2/Flotillin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-30881-img0006.jpg)
![Flow Cytometry: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of 293T cells with NPHS2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/images2/Podocin-NPHS2-Antibody-(JB51-33)-Flow-Cytometry-NBP2-75624-img0001.jpg)
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of human kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/Podocin-NPHS2-Antibody-(JB51-33)-Immunohistochemistry-Paraffin-NBP2-75624-img0002.jpg)
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of mouse kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/Podocin-NPHS2-Antibody-(JB51-33)-Immunohistochemistry-Paraffin-NBP2-75624-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SRI Antibody [NBP2-24479] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-SRI at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/SRI-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24479-img0003.jpg)
![Immunohistochemistry-Paraffin: SRI Antibody [NBP2-24479] - Analysis of a FFPE tissue section of human small intestine using 1:200 dilution of TRIM41 antibody (NBP2-24479). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Cytosplasmic staing of the glandular cells was observed.](http://images.novusbio.com/images2/SRI-Antibody-Immunohistochemistry-Paraffin-NBP2-24479-img0004.jpg)
![Western Blot: SR1 Antibody [NBP2-24479] - Analysis of human Sorcin in human brain lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain using this antibody.](http://images.novusbio.com/images2/SR1-Antibody-Western-Blot-NBP2-24479-img0002.jpg)
