ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody - BSA Free

Western Blot: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody [NBP2-98478] - Anti-ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 44 kDa

Immunoprecipitation: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody [NBP2-98478] - ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 was immunoprecipitated using: Lane A: 0.5 mg U251MG Whole Cell Lysate 1 ul anti-ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 45 kDa

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ST3GAL2
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-2 uL/mg of lysate
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody - BSA Free

• Alpha 2,3-ST 2
• Beta-galactoside alpha-2,3-sialyltransferase 2
• beta-galactoside alpha-2,3-sialytransferase
• CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2
• EC 2.4.99.4
• EC 2.4.99.7
• Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase
• Gal-NAc6S
• sialyltransferase 4B (beta-galactosidase alpha-2,3-sialytransferase)
• Sialyltransferase 4B
• SIAT4B
• SIAT4-B
• ST3 beta-galactoside alpha-2,3-sialyltransferase 2
• ST3GAL2
• ST3GalA.2
• ST3GalA.2ST3Gal II
• ST3GALII

Background

ST3GAL2 is encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ST3GAL2