SSX4 Antibody (3E10) - Azide and BSA Free

Western Blot: SSX4 Antibody (3E10) [H00006759-M02] - Western Blot detection against Immunogen (36.52 KDa) .

Immunocytochemistry/Immunofluorescence: SSX4 Antibody (3E10) [H00006759-M02] - Analysis of monoclonal antibody to SSX4 on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: SSX4 Antibody (3E10) [H00006759-M02] - Detection limit for recombinant GST tagged SSX4 is approximately 3ng/ml as a capture antibody.

Sandwich ELISA: SSX4 Antibody (3E10) [H00006759-M02] - Detection limit for recombinant GST tagged SSX4 is 0.03 ng/ml as a capture antibody.

Sandwich ELISA: SSX4 Antibody (3E10) [H00006759-M02] - Detection limit for recombinant GST tagged SSX4 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SSX4 Antibody (3E10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: SSX4 (NP_005627, 91 a.a. - 188 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RNQVERPQMTFGSLQRIFPKIMPKKPAEEENGLKEVPEASGPQNDGKQLCPPGNPSTLEKINKTSGPKRGKHAWTHRLRERKQLVVYEEISDPEEDDE
• Specificity: SSX4 - synovial sarcoma, X breakpoint 4
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SSX4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SSX4 Antibody (3E10) - Azide and BSA Free

• Cancer/testis antigen 5.4
• CT5.4SSX4B
• MGC119056
• MGC12411
• protein SSX4
• SSX4A
• synovial sarcoma, X breakpoint 4

Background

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. Chromosome Xp11 contains a segmental duplication resulting in two identical copies of synovial sarcoma, X breakpoint 4, SSX4 and SSX4B, in tail-to-tail orientation. This gene, SSX4, represents the more telomeric copy. Two transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SSX4
• Entrez:
  • 6759(Human)
• OMIM:
  • 300326(Human)
• Uniprot:
  • NP_005627(Human)